Eph Receptor A1 (EPHA1) (NM 005232) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC213689

Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone Tag: Myc-DDK Symbol: EPHA1 Synonyms: EPH; EPHT; EPHT1 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 6 Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone – RC213689

ORF Nucleotide >RC213689 representing NM_005232 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGGAGCGGCGCTGGCCCCTGGGGCTAGGGCTGGTGCTGCTGCTCTGCGCCCCGCTGCCCCCGGGGGCTC ACGCCAAGGAAGTTACTCTGATGGACACAAGCAAGGCACAGGGAGAGCTGGGCTGGCTGCTGGATCCCCC AAAAGATGGGTGGAGTGAACAGCAACAGATACTGAATGGGACACCCCTGTACATGTACCAGGACTGCCCA ATGCAAGGACGCAGAGACACTGACCACTGGCTTCGCTCCAATTGGATCTACCGCGGGGAGGAGGCTTCCC GCGTCCACGTGGAGCTGCAGTTCACCGTGCGGGACTGCAAGAGTTTCCCTGGGGGAGCCGGGCCTCTGGG CTGCAAGGAGACCTTCAACCTTCTGTACATGGAGAGTGACCAGGATGTGGGCATTCAGCTCCGACGGCCC TTGTTCCAGAAGGTAACCACGGTGGCTGCAGACCAGAGCTTCACCATTCGAGACCTTGCGTCTGGCTCCG TGAAGCTGAATGTGGAGCGCTGCTCTCTGGGCCGCCTGACCCGCCGTGGCCTCTACCTCGCTTTCCACAA CCCGGGTGCCTGTGTGGCCCTGGTGTCTGTCCGGGTCTTCTACCAGCGCTGTCCTGAGACCCTGAATGGC TTGGCCCAATTCCCAGACACTCTGCCTGGCCCCGCTGGGTTGGTGGAAGTGGCGGGGACCTGCTTGCCCC ACGCGCGGGCCAGCCCCAGGCCCTCAGGTGCACCCCGCATGCACTGCAGCCCTGATGGCGAGTGGCTGGT GCCTGTAGGACGGTGCCACTGTGAGCCTGGCTATGAGGAAGGTGGCAGTGGCGAAGCATGTGTTGCCTGC CCTAGCGGCTCCTACCGGATGGACATGGACACACCCCATTGTCTCACGTGCCCCCAGCAGAGCACTGCTG AGTCTGAGGGGGCCACCATCTGTACCTGTGAGAGCGGCCATTACAGAGCTCCCGGGGAGGGCCCCCAGGT GGCATGCACAGGTCCCCCCTCGGCCCCCCGAAACCTGAGCTTCTCTGCCTCAGGGACTCAGCTCTCCCTG CGTTGGGAACCCCCAGCAGATACGGGGGGACGCCAGGATGTCAGATACAGTGTGAGGTGTTCCCAGTGTC AGGGCACAGCACAGGACGGGGGGCCCTGCCAGCCCTGTGGGGTGGGCGTGCACTTCTCGCCGGGGGCCCG GGGGCTCACCACACCTGCAGTGCATGTCAATGGCCTTGAACCTTATGCCAACTACACCTTTAATGTGGAA GCCCAAAATGGAGTGTCAGGGCTGGGCAGCTCTGGCCATGCCAGCACCTCAGTCAGCATCAGCATGGGGC ATGCAGAGTCACTGTCAGGCCTGTCTCTGAGACTGGTGAAGAAAGAACCGAGGCAACTAGAGCTGACCTG GGCGGGGTCCCGGCCCCGAAGCCCTGGGGCGAACCTGACCTATGAGCTGCACGTGCTGAACCAGGATGAA GAACGGTACCAGATGGTTCTAGAACCCAGGGTCTTGCTGACAGAGCTGCAGCCTGACACCACATACATCG TCAGAGTCCGAATGCTGACCCCACTGGGTCCTGGCCCTTTCTCCCCTGATCATGAGTTTCGGACCAGCCC ACCAGTGTCCAGGGGCCTGACTGGAGGAGAGATTGTAGCCGTCATCTTTGGGCTGCTGCTTGGTGCAGCC TTGCTGCTTGGGATTCTCGTTTTCCGGTCCAGGAGAGCCCAGCGGCAGAGGCAGCAGAGGCAGCGTGACC GCGCCACCGATGTGGATCGAGAGGACAAGCTGTGGCTGAAGCCTTATGTGGACCTCCAGGCATACGAGGA CCCTGCACAGGGAGCCTTGGACTTTACCCGGGAGCTTGATCCAGCGTGGCTGATGGTGGACACTGTCATA GGAGAAGGAGAGTTTGGGGAAGTGTATCGAGGGACCCTGAGGCTCCCCAGCCAGGACTGCAAGACTGTGG CCATTAAGACCTTAAAAGACACATCCCCAGGTGGCCAGTGGTGGAACTTCCTTCGAGAGGCAACTATCAT GGGCCAGTTTAGCCACCCGCATATTCTGCATCTGGAAGGCGTCGTCACAAAGCGAAAGCCGATCATGATC ATCACAGAATTTATGGAGAATGGAGCCCTGGATGCCTTCCTGAGGGAGCGGGAGGACCAGCTGGTCCCTG GGCAGCTAGTGGCCATGCTGCAGGGCATAGCATCTGGCATGAACTACCTCAGTAATCACAATTATGTCCA CCGGGACCTGGCTGCCAGAAACATCTTGGTGAATCAAAACCTGTGCTGCAAGGTGTCTGACTTTGGCCTG ACTCGCCTCCTGGATGACTTTGATGGCACATACGAAACCCAGGGAGGAAAGATCCCTATCCGTTGGACAG CCCCTGAAGCCATTGCCCATCGGATCTTCACCACAGCCAGCGATGTGTGGAGCTTTGGGATTGTGATGTG GGAGGTGCTGAGCTTTGGGGACAAGCCTTATGGGGAGATGAGCAATCAGGAGGTTATGAAGAGCATTGAG GATGGGTACCGGTTGCCCCCTCCTGTGGACTGCCCTGCCCCTCTGTATGAGCTCATGAAGAACTGCTGGG CATATGACCGTGCCCGCCGGCCACACTTCCAGAAGCTTCAGGCACATCTGGAGCAACTGCTTGCCAACCC CCACTCCCTGCGGACCATTGCCAACTTTGACCCCAGGGTGACTCTTCGCCTGCCCAGCCTGAGTGGCTCA GATGGGATCCCGTATCGAACCGTCTCTGAGTGGCTCGAGTCCATACGCATGAAACGCTACATCCTGCACT TCCACTCGGCTGGGCTGGACACCATGGAGTGTGTGCTGGAGCTGACCGCTGAGGACCTGACGCAGATGGG AATCACACTGCCCGGGCACCAGAAGCGCATTCTTTGCAGTATTCAGGGATTCAAGGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 6 Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone – RC213689

Protein Sequence: >RC213689 representing NM_005232 Red=Cloning site Green=Tags(s)

MERRWPLGLGLVLLLCAPLPPGAHAKEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCP MQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRP LFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNG LAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVAC PSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSL RWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVE AQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDE ERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGEIVAVIFGLLLGAA LLLGILVFRSRRAQRQRQQRQRDRATDVDREDKLWLKPYVDLQAYEDPAQGALDFTRELDPAWLMVDTVI GEGEFGEVYRGTLRLPSQDCKTVAIKTLKDTSPGGQWWNFLREATIMGQFSHPHILHLEGVVTKRKPIMI ITEFMENGALDAFLREREDQLVPGQLVAMLQGIASGMNYLSNHNYVHRDLAARNILVNQNLCCKVSDFGL TRLLDDFDGTYETQGGKIPIRWTAPEAIAHRIFTTASDVWSFGIVMWEVLSFGDKPYGEMSNQEVMKSIE DGYRLPPPVDCPAPLYELMKNCWAYDRARRPHFQKLQAHLEQLLANPHSLRTIANFDPRVTLRLPSLSGS DGIPYRTVSEWLESIRMKRYILHFHSAGLDTMECVLELTAEDLTQMGITLPGHQKRILCSIQGFKD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Chromatograms: https://cdn.origene.com/chromatograms/mk6167_a05.zip Restriction Sites: SgfI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 6 Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone – RC213689

Plasmid Map:

ACCN: NM_005232 ORF Size: 2928 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_005232.5 RefSeq Size: 3367 bp RefSeq ORF: 2931 bp Locus ID: 2041 UniProt ID: P21709 Protein Families: Druggable Genome, Protein Kinase, Transmembrane Protein Pathways: Axon guidance MW: 107.9 kDa

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 6 Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone – RC213689

Gene Summary: This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis. [provided by RefSeq, Jul 2008]

Product images:

HEK293T cells were transfected with the pCMV6- ENTRY control (Cat# [PS100001], Left lane) or pCMV6-ENTRY EPHA1 (Cat# RC213689, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS- PAGE and immunoblotted with anti-EPHA1 (1:2000) (Cat# [TA809666]). Positive lysates [LY401608] (100ug) and [LC401608] (20ug) can be purchased separately from OriGene.

Western blot validation of overexpression lysate (Cat# [LY401608]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213689 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 5 / 6 Eph receptor A1 (EPHA1) (NM_005232) Human Tagged ORF Clone – RC213689

Coomassie blue staining of purified EPHA1 protein (Cat# [TP313689]). The protein was produced from HEK293T cells transfected with EPHA1 cDNA clone (Cat# RC213689) using MegaTran 2.0 (Cat# [TT210002]).