SYMPOSIUM TARRAGONA 1 St / 4 Th APRIL 2019
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Improvement of Late Blight Resistance on Potato by Transgenesis and Cisgenesis
Improvement of late blight resistance on potato by transgenesis and cisgenesis Name: Tiantian Liu Registration number: 860927523040 Examiners: Prof. Evert Jacobsen & Dr. Jack Vossen Supervision: MSc Suxian Zhu & Dr. Jack Vossen Course code: PBR-80436 Thesis Plant Breeding Study specialisation: Plant Breeding and Genetic Resources Abstract This project aimed at the improvement of late blight resistance in potato by means of transgenesis and cisgenesis. In transgenesis, Rpi-mcq1 was successfully cloned into vector pBINPLUS. After single R gene transformation or by transformation of multiple different R genes, positive transformants were detected by PCR and functional assays in cisgenesis. Different transformation approaches were followed in this project in order to find out an efficient method for generating marker free plants. The results indicated that co-transformation of selection marker containing and marker free constructs is much more efficient in generating R gene positive plants than transformation of marker free constructs alone. However, several cisgenic plants were also selected from marker free transformation. Moreover, in vitro disease assay could be efficiently used as a pre-selection for positive R-gene containing regenerants. Keywords: potato; marker free; (co-)transformation; R-gene; functionality i Thesis Outline To improve the late blight resistance in potato by cisgenesis, three experiments were carried out. 1. To build up a Desiree transformants differential set, Rpi-mcq1 gene was cloned from vector pCLD04541 with the amplification primers which were designed by removing some nucleotides to reduce the length of gene from the patent (Jones et al., 2010). It was transferred into the vector pBINPLUS. 2. In order to get sufficient numbers of cisgenic plants, marker free transformation with two R genes, Rpi-sto1 and Rpi-blb3 , was carried out in previous experiments. -
The Deadlock in European GM Crop Authorisations As a Wicked Problem by Design
The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Ruth Mampuys The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Ruth Mampuys Colofon Sociology, Theory and Methodology | Erasmus School of Law | 2020 Author: Ruth Mampuys Thesis design & layout: Bart Erkamp Cover design: Matteo Bettoni The Deadlock in European GM Crop Authorisations as a Wicked Problem by Design A need for Repoliticisation of the Decision-making Process Thesis To obtain the degree of Doctor from the Erasmus University Rotterdam By command of the rector magnificus Prof.dr. F.A. van der Duijn Schouten and in accordance with the decision of the Doctorate Board. The public defence shall be held on Thursday 28 january 2021 at 15:30 hrs by Ruth Mampuys born in Enschede, the Netherlands Doctoral Committee Promotors: Prof. dr. W. van der Burg Prof. dr. F.W.A. Brom Other members: Prof. dr. A. Arcuri Prof. dr. K. Millar Prof. dr. J.E.J. Prins Copromotor: Dr. L.M. Poort CONTENTS PREFACE 1 LIST OF ABBREVIATIONS AND ACRONYMS 5 CHAPTER 1 Biotechnology governance: why, how and by whom? 9 1. Introduction 11 2. Varying definitions of biotechnology and GMOs 14 3. Recurring themes in discussions about biotechnology 17 3.1 Fundamental moral perspectives 18 3.2 Attitudes on risks/benefits 19 3.3 Broader issues 20 4. Regulatory framework for GMOs in Europe 21 4.1 Prerequisite: an environmental risk and food safety assessment 24 4.2 Regulatory decision-making: Comitology 25 4.3 Decision-making in practice 30 5. -
Lay and Scientific Categorizations of New Breeding Techniques: Implications for Food Policy and Genetically Modified Organism Legislation
1 Public Understanding of Science Archimer July 2020, Volume 29, Issue 5, Pages 524-543 https://doi.org/10.1177/0963662520929668 https://archimer.ifremer.fr https://archimer.ifremer.fr/doc/00634/74604/ Lay and scientific categorizations of new breeding techniques: Implications for food policy and genetically modified organism legislation Debucquet Gervaise 1, * , Baron Regis 2, Cardinal Mireille 3 1 AUDENCIA Business School, France 2 Unité Biotechnologies et Ressources Marines, IFREMER, Rue de l’Ile d’Yeu, France 3 Laboratoire Ecosystèmes Microbiens et Molécules Marines pour les Biotechnologies (EM3B), IFREMER, Rue de l’Ile d’Yeu, France * Corresponding author : Gervaise Dubucquet, email address : [email protected] Abstract : The rapid development of new genetic breeding techniques is accompanied by a polarized debate around their risks. Research on the public perception of these techniques lags behind scientific developments. This study tests a method for revealing laypeople’s perceptions and attitudes about different genetic techniques. The objectives are to enable laypeople to understand the key principles of new genetic breeding techniques and to permit a comparison of their modes of classification with those of scientific experts. The combined method of a free sorting task and focus groups showed that the participants distinguished the techniques that did not induce any change in DNA sequence, and applied two different logics to classify the other breeding techniques: a Cartesian logic and a naturalistic logic with a distinct set of values. The lay categorization differed substantially from current scientific categorizations of genetic breeding techniques. These findings have implications for food innovation policy and genetically modified organism legislation. Keywords : food policy, genetically modified organism regulation, genetically modified organisms, lay categorization, new breeding techniques, and public understanding Please note that this is an author-produced PDF of an article accepted for publication following peer review. -
Metabolic Engineering Using Iterative Self-Cloning to Improve Lipid
www.nature.com/scientificreports OPEN Metabolic engineering using iterative self-cloning to improve lipid productivity in Coccomyxa Received: 14 December 2017 Yuki Kasai1,2, Takuya Tsukahara1, Fukiko Ikeda1, Yoko Ide1,2,3 & Shigeaki Harayama1,2 Accepted: 26 July 2018 We previously developed a self-cloning system that introduces cDNA of the uridine monophosphate Published: xx xx xxxx synthase gene (cUMPS) of Coccomyxa sp. strain Obi as a selectable marker into uracil-auxotrophic mutants (Ura−) of the same alga. Here, we developed a Cre/loxP-based system for the removal of cUMPS fanked by directly repeated loxP sites from the Coccomyxa genome using the intracellular delivery of purifed Cre recombinase to generate an Ura− strain that was used as a host for second-round transformation using cUMPS as the selection marker. Employing this marker–gene-recycling system, Coccomyxa strains devoid of foreign DNA except the 34-bp loxP sequence, which overexpressed an acyl-(acyl-carrier-protein) thioesterase gene, and a type-2 diacylglycerol acyltransferase gene, were constructed by the sequential introduction of two expression cassettes for the respective genes. One of the resulting strains showed 1.4-fold higher lipid productivity than the wild-type strain. This method will be applicable to other eukaryotic microalgae to create marker-free transgenic strains. Increase in greenhouse gas emissions and associated global warming has led to growing interest in the develop- ment and production of biofuels. Microalgae have long been recognized as a potential source for biofuel produc- tion because of high oil content, high growth rate, and non-requirement of arable land for cultivation1. -
RHS Groet Die Gras Super Rugby – Rustenburg – Ná Jare Van Geldinsameling En Grasoppervlak Van Die A-Hokkieveld Gegroet
22 July 2016, PLATINUM WEEKLY, 50 Marais Street, Tel: 014 592 9686, Fax: 011 252 6669, E-mail: [email protected] p 15 Vodacom RHS groet die gras Super Rugby – Rustenburg – Ná jare van geldinsameling en grasoppervlak van die A-hokkieveld gegroet. skool, sê hulle is dankbaar vir die groot Week 17 borge soek, spog die Hoërskool Rustenburg Die waterbasis-astrohokkieveld het ʼn hoë borge wat hierdie projek moontlik gemaak uiteindelik met ‘n splinternuwe astrohokkieveld kunsmatige grasdigtheid met geen sand het. Sy sê nie al die bydraes is finansieel op Olimpiese spele standaarde. Die baan as vulling nie. Dit verseker die konstante nie. “Ondersteuning was ook gebied deur is die afgelope vakansie voltooi – en in ‘n beweging van die bal. Die oppervlak is ten mense wat hul tyd daaraan afgestaan het of japtrap het die ouens en hokkie bokkies hul volle besproei met water wat lei tot ʼn baie masjinerie en materiaal aangebied het vir die vernuf op díe nuwe veld kom toets. vinnige en professionele spel. projek.” Die trotse Mnr Johan Smit, RHS se Hoof, As gevolg van die afwesigheid van sand kan Die paviljoen sal mettertyd uitgebrei word en Blues vs Waratahs vertel aan die Platinum Weekly koerant dat díe die kunsmatige gras vrylik beweeg wat die parkering sal by die baan ingerig word sodat Die Blues slaag daarin om in Auckland reuse projek beslis nie sonder die toegewyde speler in staat stel om hul hokkiestok onder dit makliker deur die breë gemeenskap benut nog ‘n Aussie span die loef af te steek en ondersteuning van die gemeenskap ooit ‘n die bal te sit. -
Announcement
Announcement Total 100 articles, created at 2016-08-26 00:02 1 Donald Trump calls Hillary Clinton a 'bigot' at Mississippi rally – video (1.39/2) Republican presidential nominee Donald Trump spoke to a rally in Mississippi on Wednesday evening and called political rival Hillary Clinton ‘a bigot’ 2016-08-25 16:02 1KB www.theguardian.com 2 Trump proclaims polls with black and Hispanic voters have gone 'way up' (1.02/2) Republican presidential candidate Donald Trump told rally-goers in Tampa on Aug. 24 that "the polls with African American folks and Spanish-speaking folks, the Hispanics, Latinos, have gone way up" in the last three weeks. 2016-08-25 18:26 975Bytes www.washingtonpost.com 3 Woman Dies After Falling 40 Feet From Zip Line in Delaware Video (1.02/2) A 59-year-old woman has died after falling approximately 40 feet from a zip line at the Lums Pond State Park in Delaware. 2016-08-25 18:23 1KB abcnews.go.com 4 Turkey warns Syrian Kurds to withdraw east of Euphrates (1.02/2) Turkey threatens further intervention in northern Syria unless Kurdish-led forces withdraw east of the River Euphrates within a week. 2016-08-25 18:03 3KB www.bbc.co.uk 5 WATCH: Black Coffee's son left overwhelmed by lesson on how to take care of a lady Black Coffee's young boy appears to be overwhelmed when his dad explains to him all the things that go into taking care of a lady, saying 2016-08-26 00:01 1KB www.timeslive.co.za 6 Memorial service for Inchanga shooting victims called off to allow for peace talks A joint memorial for two victims of factional fighting in Inchanga was postponed early on Thursday. -
SCORERS - Page 1
2015 - SUPER RUGBY - SCORERS - Page 1 Tries scored by Team # Points scored by Team # Penalty Tries Various 14 Lima Sopoaga Highlanders 191 Waisake Naholo Highlanders 13 Bernard Foley Waratahs 187 TJ Perenara Hurricanes 11 Demetri Catrakilis Stormers 172 Nemani Nadolo Crusaders 9 Christian Lealiifano Brumbies 170 Boom Prinsloo Cheetahs 8 Handré Pollard Bulls 167 David Pocock Brumbies 8 Elton Jantjies Lions 152 Joe Tomane Brumbies 8 Daniel Carter Crusaders 127 Julian Savea Hurricanes 8 Mike Harris Rebels 123 Patrick Osborne Highlanders 8 Beauden Barrett Hurricanes 121 Teqele Naiyaravoro Waratahs 8 Ihaia West Blues 104 Aaron Smith Highlanders 7 Colin Slade Crusaders 100 Francois Venter Cheetahs 7 Aaron Cruden Chiefs 93 Matt Todd Crusaders 7 Patrick Lambie Sharks 93 Rob Horne Waratahs 7 Joe Pietersen Cheetahs 92 Charlie Ngatai Chiefs 6 Penalty Tries Various 70 Dillyn Leyds Stormers 6 Waisake Naholo Highlanders 65 Francois Hougaard Bulls 6 James Marshall Hurricanes 62 Lachie Turner Reds 6 Kurt Coleman Stormers 55 Samu Kerevi Reds 6 TJ Perenara Hurricanes 55 Adam Ashley-Cooper Waratahs 5 Luke Burton Force 47 Ben Smith Highlanders 5 Nemani Nadolo Crusaders 45 Bernard Foley Waratahs 5 James O'Connor Reds 44 Cornal Hendricks Cheetahs 5 Jacques-Louis Potgieter Bulls 42 Faf de Klerk Lions 5 Quade Cooper Reds 42 Israel Folau Waratahs 5 Damian McKenzie Chiefs 41 James Lowe Chiefs 5 Daniel Bowden Blues 41 Jan Serfontein Bulls 5 Boom Prinsloo Cheetahs 40 Luke Morahan Force 5 David Pocock Brumbies 40 Lwazi Mvovo Sharks 5 Joe Tomane Brumbies 40 Ma'a Nonu -
Sponsors' Brochure Sponsors
BIGGAR RFC SPONSORS’ BROCHURE SEASON 2018/2019 ONE CLUB ONE FAMILY President’s Message It is again an honour for me to be writing this year’s sponsorship message as I am writing it as the championship winning president of Biggar rugby club. It was an incredible season of success throughout the club, the Under 16 boys going to the National final at Murrayfield but on the day were narrowly beaten by Stirling County but did win the West Regional cup. Our under 18 girls again achieved a Murrayfield final and again won for a fifth year. The senior season was capped by the First XV winning their league as champions, promoting the club to National 1, for this season 2019/2020. The season could hardly have got any better, it did, Biggar rugby football club were presented with “The Club of the Year Award at the annual SRU awards dinner at the Corn Exchange. What a special finish to a fantastic season! Our club has strengthened its position as a hub in our community for numerous sports clubs. We are delighted to remain hosts to Biggar United and Law AAC at Hartreemill for training and competition. We at Biggar RFC are lucky enough to have 17 squads of both male and female players ranging from the new Biggar Bairns (rugby for 3-5-year olds) to over 35s. Many businesses and individuals from the Biggar area and the extended community support our club with their sponsorship and advertising, without this support we could not offer our children, youth and senior playing squads and other sports clubs the facilities we enjoy. -
Gmo Mod-Bio Ngt Eu-Study.Pdf
EUROPEAN COMMISSION Brussels, 29.4.2021 SWD(2021) 92 final COMMISSION STAFF WORKING DOCUMENT Study on the status of new genomic techniques under Union law and in light of the Court of Justice ruling in Case C-528/16 EN EN Contents 1. Executive summary ......................................................................................................................... 2 2. Background and objectives of the Commission study on new genomic techniques ...................... 5 2.1. Council request for a Commission study ........................................................................................... 5 2.2. Scope and objectives of the study..................................................................................................... 6 3. Methodology of the study ............................................................................................................... 7 3.1 General methodology ....................................................................................................................... 7 3.2 Targeted consultations ...................................................................................................................... 7 3.3 Overview of NGT legislation in non-EU countries ............................................................................. 8 3.4 State of the art on NGTs .................................................................................................................... 8 3.5 Overview of EU NGT research funding ............................................................................................. -
Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques
sustainability Review Effect of Organic Potato Farming on Human and Environmental Health and Benefits from New Plant Breeding Techniques. Is It Only a Matter of Public Acceptance? Daniela Pacifico * and Roberta Paris Council for Agricultural Research and Economics Analysis (CREA), Centre for Research on Industrial Crops, Via di Corticella, 133, 40128 Bologna, Italy; [email protected] * Correspondence: daniela.pacifi[email protected]; Tel.: +39-051-631-6811 Academic Editor: Gerhart U. Ryffel Received: 26 July 2016; Accepted: 13 October 2016; Published: 20 October 2016 Abstract: Organic farming practices are commonly thought to reduce the environmental impact of agriculture and to preserve the naturalness of the products. Herein, we report the effect of crop management practices on nutritional and toxicological value of potato tubers. Comparative studies are often controversial and the results are dependent on genotype and methodological approach. Targeted analysis and “omics” strategies are discussed, pointing at the nutritional aspects and the corresponding biological and molecular processes involved. Organic farming supporters still do not accept the use of genetic modification to produce new varieties suited for organic agriculture and crop improvement by genetic engineering still sparks hot debate among various scientific and social factions whose major concern is the possible existence of unintended effects both on human and world health. In this context, the advent of “new plant breeding techniques” has reignited the discussion on genetic engineering and on the compatibility of the new technologies with an eco-friendly agriculture. Could cisgenic and genome-edited potatoes be new good options for organic agriculture? We discuss how these approaches can be used to address food security challenges and to overcome specific problems based on the biological characteristics of potato tubers, producing new varieties that can improve farmers’ profit with a lower impact on public opinion. -
Match Summary
MATCH SUMMARY TEAMS Cell C Sharks vs DHL Western Province VENUE Growthpoint Kings Park DATE 14 October 2017 15:00 COMPETITION Currie Cup Premier Division FINAL SCORE 20 - 31 HALFTIME SCORE 13 - 3 TRIES 2 - 4 PLAYER OF THE MATCH SCORING SUMMARY Cell C Sharks DHL Western Province PLAYER T C P DG PLAYER T C P DG Curwin Bosch (J #10) 0 2 1 1 Jd Schickerling (J #5) 1 0 0 0 Marius Louw (J #12) 1 0 0 0 Dewaldt Duvenage (J #9) 1 0 0 0 Kobus Van Wyk (J #22) 1 0 0 0 Robert Du Preez Jnr (J #10) 2 4 1 0 LINE-UP Cell C Sharks DHL Western Province 1 Thomas Du Toit (J #1) 1 Jc Janse Van Rensburg (J #1) 2 Franco Marais (J #2) 2 Bongi Mbonambi (J #2) 3 Ross Geldenhys (J #3) 3 Wilco Louw (J #3) 4 Tyler Paul (J #4) 4 Chris Van Zyl (J #4) 5 Ruan Botha (J #5) 5 Jd Schickerling (J #5) 6 Keegan Daniel (J #6) 6 Jaco Coetzee (J #6) 7 Jacques Vermeulen (J #7) 7 Cobus Wiese (J #7) 8 Dan Du Preez (J #8) 8 Nizaam Carr (J #8) 9 Michael Claassens (J #9) 9 Dewaldt Duvenage (J #9) 10 Curwin Bosch (J #10) 10 Robert Du Preez Jnr (J #10) 11 S'bu Nkosi (J #11) 11 Dillyn Leyds (J #11) 12 Marius Louw (J #12) 12 Ruhan Nel (J #12) 13 Lukhanyo Am (J #13) 13 Huw Jones (J #13) 14 Odwa Ndungane (J #14) 14 Seabelo Senatla(dnu) (J #14) 15 Garth April (J #15) 15 Damian Willemse (J #15) RESERVES Cell C Sharks DHL Western Province 16 Chiliboy Ralepelle (J #16) 16 Ramone Samuels (J #16) 17 Juan Schoeman (J #17) 17 Caylib Oosthuizen (J #17) 18 Jean Droste (J #18) 18 Jan De Klerk (J #18) 19 Tera Mtembu (J #19) 19 Kobus Van Dyk (J #19) 20 Louis Schreuder (J #20) 20 Justin Phillips (J #20) -
2017 Agricultural Biotechnology Annual France
THIS REPORT CONTAINS ASSESSMENTS OF COMMODITY AND TRADE ISSUES MADE BY USDA STAFF AND NOT NECESSARILY STATEMENTS OF OFFICIAL U.S. GOVERNMENT POLICY Required Report - public distribution Date: 6/29/2017 GAIN Report Number: FR1714 France Agricultural Biotechnology Annual 2017 Approved By: Kate Snipes Prepared By: FAS Paris Report Highlights: Although public opinion in France is generally opposed to products derived from biotechnology, the livestock industry is dependent upon imported genetically engineered (GE) products to meet its feed needs. France has no commercial production or field trials of GE crops, but some laboratory research is being conducted in the country. The French administration is conflicted on the way innovative biotechnologies should be regulated. The seed industry and the main farm organizations have developed a detailed position in favor of innovative biotechnologies, while anti-biotech groups have conducted a few actions against them. Public awareness is low. As for animal biotechnology, it is mainly used for medical research purposes. Executive Summary: Agricultural biotechnology is a very sensitive and controversial subject in France. Anti-biotech groups actively campaign against it and they have a strong influence on public opinion, which is generally opposed to products derived from biotechnology. There is better acceptance among grain producers, animal feed compounders, and scientists. Scientists and some decision-makers express growing concern with the precedence of politics over science. In 2017, the National Assembly voted a resolution on this subject. France is active in research and uses both genetic engineering and innovative biotechnologies in labs. However, the country is more advanced in medical and industrial biotechnology than in agricultural biotechnology.