Supplementary Material Table of Contents

Text S1. Nucleic acid sequences of the PoNi gene from A. robustus, A. contortus, P. ruminantium, C. sp. Brit4, P. sp. FS3c, and R. albus.

Text S2. Amino acid sequences of PoNi proteins from A. robustus, A. contortus, P. ruminantium, C. sp. Brit4, P. sp. FS3c, and R. albus.

Table S1. Nucleic acid sequence identities between the PoNi coding sequences, presented as a percent identity matrix.

Table S2. Amino acid sequence identities between the PoNi proteins, presented as a percent identity matrix.

Table S3. Brief summary of the top hit from BLAST searches, performed using blastp with default parameters against the non-redundant protein sequences (nr).

Figure S1. Full, expanded maximum-likelihood phylogenetic tree of fungal and bacterial PoNi proteins. The fungal proteins are shown in bold. This phylogenetic tree only includes five fungal PoNi proteins, as we could not find PoNi homologs in other fungal lineages. Shimodaira-Hasegawa approximate-likelihood ratio test (aLRT SH-like) values are provided as estimates of branch support next to the nodes. GenBank accession numbers are shown in brackets. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site.

Text S1

PoNi nucleic acid sequence in Anaeromyces robustus Retrieved from Joint Genome Institute (JGI) MycoCosm website; sequenced by Haitjema et al. 2017. scaffold_304:3496-4176 ATGAGAGACACTTTAAGAACAAAGGAATATTTTGATACTTTTATTCTTGAAGAATTAGAAGATATTAAAATGTTTGA AGATAGTATTGAGAATGGGGAAATTGAAGACGAAAGAATTAATTTTATAAAAGACGATATATTAGAAATTAAATTAG GAATAATTATAGCTAAATATTCCAGAGGAGATCCTATTTGTACAATAAAAAAAGAATTTGAAGATATGATTGATTTT TTTTGTGAATTTTGGAATGGTGAAATATATGAAGATAATCTTTGGTTTGCTTCTTTGGCCTATCTTTTAAAATTAGA TGAAGCATTGTTAAAAAGAATAAGAAATAAATTAAAAGAATCTGATACATATGATTATCTTATAGATTTTATACTTA TAGGTTTTGATGATTCTCAAGATAATTTAAAAATATCATTTCCTCGTCCTTATAAAAAATTATTAAAATGTATTAAT GGTCAAGATAGGGAAGCATTATTAAAATACTTACGAGGCTGGTATAAAGGTTGTCAAGAAAGCTCATGGTATGATAC ACACAAAATTAAAGATGATAATTTATATTTCGGATATTGGTGTTTTGAAGCAGGTGCTGTAGCTAAAAGACTTGGTT TTGAAGACGATGATTTAAAAAATAAACAATATTATCCATATGATATGATTCATTATATTGAATAA

PoNi nucleic acid sequence in Anaeromyces contortus Retrieved from GenBank (nucleotide accession: GGWR01037537.1) ATGAAAACGAGAGACACTTTAAGGTCAAAGGAATATTTTGACACTTTTATTCTTGAAGAACTAGAAGATATTAAAAT GTATGAAGATAGTATTGAGAATGGGGAAATTGAAGAAGATAGAATTGATATAATAAAAGATGATATATTGGAAATCA AATTAGGAATAATTATAGCTAAATATTCAAGAGGTGATCCTATTAATATAATAAAGAAAGAGTTTGAGGATATGATT GATTTGTTTTGTGAATATTGGATTGGTGAAATATATGAAGATAATCTTTGTTTTGCTTCTTTAGCATACCTTTTAGC ATTAGATGATACCTTATTAAATAAAATAAGAAATAAATTAAAAGAATCTGATACATATGATTATCTGATAGATTTTA TATTGGTAGGTTTTGATGATGATCAAAATGATTCAAAAATATCATTTCCTCGTCCTTATAAAAAATTGTTAAAAAGT ATAAATGGTAAAGATAGAAATGCTTTTTTGAAGTATTTACGAGGTTGGTATAAAGGTTGTCAGGAAAGCTCATGGTA CGATACACACAAAATTAAAGATGACAATATATATTTTGGGTATTGGTGCTTTGAAGCAGGTGCTATAGCTAAAAGAC TTGGTTTTAAAGACGATGATTTAAAAAATGAACAGTATTATCCATACGATATGGTTCATTTTACTGAATGA

PoNi nucleic acid sequence in Pecoramyces ruminantium Retrieved from Joint Genome Institute (JGI) MycoCosm website; sequenced by Youssef et al. 2013. c_7180000049905:1398-2081 ATGAAAATGAGAGATACTTTAAGAACAAAGGAATATTTTGACACTTTTATTCAGGAAGAATTGGAAGATATTCATAT GTTTGAAGATAGTCTTTCGAAAGGAGAAATAGAGGAGGAAAGAATCGATTCGATAAAAGATGAAATATTAGAAATTA AAATGGGAATAGTTATTGCTAGATATTCAAGAGGGGATTCGATGAATGAACTAAAACAAGAATTTGAAGAAATGATT GATAGATTTTGTGAATCATGGGACGGTGAAATCTATGAGGATAATCTTTGTTTTGCCTCTTTGGCCTATCTTTTAGG ATTAGATGATGAGCAATTGAATAGAATAAGAAATAAATTAAAAGAATCGGATACCTATGATTTTCTTATAGATTTTG TACTTGTGGGTTTTGATGATACTCTGGATGAATCAAAAATATCGTTTCCACGTCCCTATAAACAATTGTTAAAGAGT ATTAATGGTAAGGATAGAGTTGCTTTCCAGAAATATCTACGAGGTTGGTATAAAGGTTGTCAGGAAAGTTCGTGGTA TGATACTCATAAAATTGAAGATGATAATCTATATTTTGGATATTGGTGCTTTGAAGCCGGTGCTGTAGCTAAAAGAC TAGGTTTTAAAGACGATGATTTAAAAAATGAGCAATATTATCCATATGATATGGTTCATTTTACATGA

PoNi nucleic acid sequence in Caecomyces sp. Brit4 Retrieved from GenBank (nucleotide accession: GGWS01028851.1 and GGWS01028853.1) ATGAAAATGAGAGACACTTTAAGGACAAAGGAATATTTTGACAAGTTTATTCTTGAAGAACTGGAAGACATTCAAAT GTTTGAAGATAGTCTTGAGAAAGGGGAAATTGAAAAAGATAGAATCGATTCAATAAAAGACGAAGTGTTACAAATTA AAGTAGGAATAATTATAGCTAAATATTCAAGAGGTGATCCTATTGATACCATAAAGAAAGAATTTGAAGATATGATT GATTCGTTTTGTGAATCTTGGGATGGTGAAATATATGAAGATAATCTTTGTTTTGCTTCCTTGGCATATCTTTTAGG ACTAGATAATACACAGCTAAATAGGATAAGAAATAAATTAAAGGAATCTGATACATACGATTATCTTATAGATTTTG TACTTGTAGGCGTTGATGATGCTCAGGATAATTCAGAAATATCATTCCCTCGTCCTTATAAACAATTATTAAAATGT ATTAATGGTGAAGACAAATATGCTTTTAAAAAATATTTACGAGGCTGGTATAAAGGTTGTCAGGAAAGTTCATGGTA TGATACACACAAAATTGAAGATGATAATTTGTATTTTGGTTATTGGTGTTTCGAAGCAGGAGCTGTAGCCAAAAGAC TTGGTTTTAAAGATGATGATTTACAAAATGAGCAGTATTATCCATATGATATGGTTCATTTTACTGAATGA

PoNi nucleic acid sequence in Piromyces sp. FS3c Retrieved from GenBank (nucleotide accession: GGXF01056490.1) ATGAGAGATACTTTAAGAACAAAGGAATATTTTGACACTTTTATTCAGGAAGAATTGGAAGATATTCATATGTTTGA AGATAGTCTTTCGAAAGGAGAAATAGAGGAGGAAAGAATCGATTCGATAAAAGATGAAATATTAGAAATTAAAATGG GAATAGTTATTGCTAGATACTCAAGAGGGGATTCGATGAATGAACTAAAACGGGAATTTGAAGAAATGATTGATAAA TTTTGTGATTCATGGGACGGTGAAATCTATGAGGATAATCTTTGTTTTGCCTCTTTGGCCTATCTTTTAGGATTAGA TGATGAGCAATTGAATAGAATAAGAAATAAATTAAAAGAATCGGATACCTATGATTTTCTTATAGATTTTGTACTTG TGGGTTTTGATGATACTCTGGATGAATCAAAAATATCGTTTCCACGTCCCTATAAACAATTGTTAAGGAGTATTAAT GGTAAGGATAGAGTTGCTTTCCAGAAATATCTACGAGGTTGGTATAAAGGTTGTCAGGAAAGCTCGTGGTATGATAC TCATAAAATTGAAGATGACAATCTATATTTTGGATATTGGTGCTTTGAAGCCGGTGCTGTAGCTAAAAGACTAGGTT TTAAAGACGATGATTTAAAAAATGAGCATTATTATCCATATGATATGGTTCATTTTGCATGA

PoNi nucleic acid sequence in Ruminococcus albus Retrieved from GenBank (nucleotide accession: ADKM02000110.1) ATGCAAATGAGAGATAAATTAAGAAAAAAAGAATACTTCGATACATTCATAGAAGAGGAGATCGAAGACATTCAGAT GTTTGAAGACAGCCTTGCCGATGGTGAGATCGAAGAAGATAGAATTGATTCAATAAAAGACGAGATACTCCTGATAA AACTTGGTGTCATTATTGCGAGATATTCAAGATGCGATCCTATAGACGATATCAAGTCGGGATTTGAAGACATGATC GATATGTTTTGCGAATCATGGGACGGTGGTATATATGAAGATAATCTATGGTTTGCATCATTGGCTTATCTTCTGGG GCTTGACAGCGCAAAACTTGAAAAAATAAGGAAAAAATTGATGGAAAGCGACACGTATGACTATCTTATTGATTTTA TCCTATCGGGTACTGAAAGCAAGTTTGACAACAGTAAGATATCTTTCCCGCGTTCATATAAAAAACTGGTCAAAAGC ATAAATGAGAATGACAAAGAGTCGTTATTAAAATATCTGCGCGGCTGGTATAAAGGCAGTCAGGAAAGTTCCTGGTA TGATACGCACAAGATCACAGACGACAACCTTTATTACGGCTACTGGTGCTTTGATGCAGGTGCAGTTGCAAAAAGGC TTGGTCTTGAAGACAGTGACCTGCAAAATGAACAGTACTATCCTTATGATATCGTTCATTTCAGCTGA

PoNi nucleic acid sequence alignment

Text S2

Expected PoNi amino acid sequence in Anaeromyces robustus MRDTLRTKEYFDTFILEELEDIKMFEDSIENGEIEDERINFIKDDILEIKLGIIIAKYSRGDPICTIKKEFEDMIDF FCEFWNGEIYEDNLWFASLAYLLKLDEALLKRIRNKLKESDTYDYLIDFILIGFDDSQDNLKISFPRPYKKLLKCIN GQDREALLKYLRGWYKGCQESSWYDTHKIKDDNLYFGYWCFEAGAVAKRLGFEDDDLKNKQYYPYDMIHYIE

Expected PoNi amino acid sequence in Anaeromyces contortus MKTRDTLRSKEYFDTFILEELEDIKMYEDSIENGEIEEDRIDIIKDDILEIKLGIIIAKYSRGDPINIIKKEFEDMI DLFCEYWIGEIYEDNLCFASLAYLLALDDTLLNKIRNKLKESDTYDYLIDFILVGFDDDQNDSKISFPRPYKKLLKS INGKDRNAFLKYLRGWYKGCQESSWYDTHKIKDDNIYFGYWCFEAGAIAKRLGFKDDDLKNEQYYPYDMVHFTE

Expected PoNi amino acid sequence in Pecoramyces ruminantium MKMRDTLRTKEYFDTFIQEELEDIHMFEDSLSKGEIEEERIDSIKDEILEIKMGIVIARYSRGDSMNELKQEFEEMI DRFCESWDGEIYEDNLCFASLAYLLGLDDEQLNRIRNKLKESDTYDFLIDFVLVGFDDTLDESKISFPRPYKQLLKS INGKDRVAFQKYLRGWYKGCQESSWYDTHKIEDDNLYFGYWCFEAGAVAKRLGFKDDDLKNEQYYPYDMVHFT

Expected PoNi amino acid sequence in Caecomyces sp. Brit4 MKMRDTLRTKEYFDKFILEELEDIQMFEDSLEKGEIEKDRIDSIKDEVLQIKVGIIIAKYSRGDPIDTIKKEFEDMI DSFCESWDGEIYEDNLCFASLAYLLGLDNTQLNRIRNKLKESDTYDYLIDFVLVGVDDAQDNSEISFPRPYKQLLKC INGEDKYAFKKYLRGWYKGCQESSWYDTHKIEDDNLYFGYWCFEAGAVAKRLGFKDDDLQNEQYYPYDMVHFTE

Expected PoNi amino acid sequence in Piromyces sp. FS3c MRDTLRTKEYFDTFIQEELEDIHMFEDSLSKGEIEEERIDSIKDEILEIKMGIVIARYSRGDSMNELKREFEEMIDK FCDSWDGEIYEDNLCFASLAYLLGLDDEQLNRIRNKLKESDTYDFLIDFVLVGFDDTLDESKISFPRPYKQLLRSIN GKDRVAFQKYLRGWYKGCQESSWYDTHKIEDDNLYFGYWCFEAGAVAKRLGFKDDDLKNEHYYPYDMVHFA

Expected PoNi amino acid sequence in Ruminococcus albus GenBank accession (nucleotide): ADKM02000110.1; GenBank accession (protein): EGC02125.1 MQMRDKLRKKEYFDTFIEEEIEDIQMFEDSLADGEIEEDRIDSIKDEILLIKLGVIIARYSRCDPIDDIKSGFEDMI DMFCESWDGGIYEDNLWFASLAYLLGLDSAKLEKIRKKLMESDTYDYLIDFILSGTESKFDNSKISFPRSYKKLVKS INENDKESLLKYLRGWYKGSQESSWYDTHKITDDNLYYGYWCFDAGAVAKRLGLEDSDLQNEQYYPYDIVHFS PoNi amino acid sequence alignment

Supplementary Tables

Table S1. Nucleic acid sequence identities between the PoNi coding sequences, presented as a percent identity matrix. A. A. O. sp. P. C. sp. P. sp. R. albus robustus contortus strain ruminantium Brit4 FS3c C1A A. robustus 100.00% 88.36% 84.06% 84.06% 86.32% 83.70% 72.08% A. contortus 88.36% 100.00% 84.28% 84.28% 87.34% 82.97% 72.49% Pecoramyces ruminantium 84.06% 84.28% 100.00% 100.00% 84.86% 97.66% 73.39% C. sp. Brit4 86.32% 87.34% 84.86% 84.86% 100.00% 82.82% 74.09% P. sp. FS3c 83.70% 82.97% 97.66% 97.66% 82.82% 100.00% 72.81% R. albus 72.08% 72.49% 73.39% 73.39% 74.09% 72.81% 100.00%

Table S2. Amino acid sequence identities between the PoNi proteins, presented as a percent identity matrix. A. robustus A. O. sp. P. C. sp. P. sp. R. albus contortus strain ruminantium Brit4 FS3c C1A A. robustus 100.00% 84.65% 78.41% 78.41% 81.14% 77.43% 73.57% A. contortus 84.65% 100.00% 82.46% 82.46% 83.33% 80.26% 72.81% Pecoramyces 78.41% 82.46% 100.00% 100.00% 85.09% 96.48% 73.13% ruminantium C. sp. Brit4 81.14% 83.33% 85.09% 85.09% 100.00% 82.46% 75.44% P. sp. FS3c 77.43% 80.26% 96.48% 96.48% 82.46% 100.00% 71.37% R. albus 73.57% 72.81% 73.13% 73.13% 75.44% 71.37% 100.00%

Table S3. Brief summary of the top hit from BLAST searches, performed using blastp with default parameters against the non-redundant protein sequences (nr). Query (Supplementary Text Top non-AGF hit Coverage E-value Identity S2) accession Anaeromyces robustus EGC02125.1 99% 1e-115 74.55% (Ruminococcus albus) Anaeromyces contortus EGC02125.1 99% 8e-115 73.13% (Ruminococcus albus) Pecoramyces ruminantium EGC02125.1 100% 1e-118 73.13% (Ruminococcus albus) Caecomyces sp. Brit4 EGC02125.1 99% 7e-121 75.77% (Ruminococcus albus) Piromyces sp. FS3c EGC02125.1 100% 1e-115 72.00% (Ruminococcus albus)

Supplementary Figure

Figure S1. Full, expanded maximum-likelihood phylogenetic tree of fungal and bacterial PoNi proteins. The fungal proteins are shown in bold. This phylogenetic tree only includes five fungal PoNi proteins, as we could not find PoNi homologs in other fungal lineages. Shimodaira-Hasegawa approximate-likelihood ratio test (aLRT SH-like) values are provided as estimates of branch support next to the nodes. GenBank accession numbers are shown in brackets. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. Curtobacterium sp. MCBA15 009 [OII13812.1] unclassified Curtobacterium [WP_139195914.1] Curtobacterium sp. MCBA15 003 [OIH96752.1] unclassified Curtobacterium [WP_071253222.1] 0%unclassified Curtobacterium [WP_181436797.1] 0%unclassified Curtobacterium [WP_082509793.1] 0%Curtobacterium sp. MCLR17 054 [WP_111054576.1] Curtobacterium sp. Leaf154 [KQR26733.1] 99.5% Marinobacter sp. ANT B65 [WP_179397133.1] Marinobacter sp. ANT B65 [PCM44857.1] Stenotrophomonas [WP_128987823.1] 75.4% 81.1% Stenotrophomonas maltophilia [PZT34079.1] 0% Stenotrophomonas maltophilia [VUN42731.1] 0% 0%Stenotrophomonas maltophilia [VUP86785.1] 91.5% 0%Stenotrophomonas maltophilia [WP_053518192.1] 0%Stenotrophomonas maltophilia [WP_181452091.1] Stenotrophomonas maltophilia [WP_065181195.1] Stenotrophomonas maltophilia [WP_080375294.1] 96.3% 72.3% 0%Stenotrophomonas maltophilia [WP_080376014.1] 0%Stenotrophomonas maltophilia [WP_164241149.1] 92.7%Stenotrophomonas maltophilia [WP_081279711.1] Stenotrophomonas sp. MA5 [WP_128987824.1] 99.7% Pseudomonas flavescens [WP_179539610.1] 76.8% Pseudomonas punonensis [WP_083593319.1] 72.1% Pseudomonas argentinensis [WP_074885923.1] 0% Burkholderia ubonensis [WP_080424890.1] Cellvibrio sp. PSBB006 [WP_087467084.1] Ruminococcus flavefaciens [WP_075424076.1] Aeromonas aquatica [WP_081966041.1] 85.9%Aeromonas hydrophila [WP_025326625.1] Aeromonas hydrophila 4AK4 [AHE49137.1] 97.4% Aeromonas [WP_076362875.1] 0% Aeromonas bestiarum [WP_043141090.1] 0% Aeromonas bestiarum [WP_043556117.1] 0% 0% Aeromonas hydrophila [ALQ65749.1] 0% 0% Aeromonas hydrophila [KWR69527.1] 0% Aeromonas hydrophila [WP_080697997.1] 0% Aeromonas hydrophila [WP_081086224.1] 0% Aeromonas hydrophila [WP_085735176.1] 0% Aeromonas hydrophila [WP_101149233.1] 0% Aeromonas hydrophila [WP_124252870.1] 83.4%0% 0%Aeromonas hydrophila [WP_130632567.1] 0%Aeromonas sp. CA23 [WP_098983847.1] Aeromonas sp. MDS8 [WP_026459185.1] Aeromonas bivalvium [WP_084205528.1] 59.8% 92.9% Shewanella marina [WP_025822619.1] 83.3% Vibrio spartinae [WP_074371159.1] 41.9%Vibrio spartinae [SIO92516.1] 76.6% Vibrio ruber [WP_077334980.1] Caballeronia turbans [SAL32183.1] 0% 91.4% Leptothrix sp. [MBA4109028.1] 66.7% Pseudomonas plecoglossicida [WP_108481614.1] 91.5% 80.3% Pseudomonas [WP_010954842.1] 0% Pseudomonas putida [RFQ04329.1] 0% Pseudomonas putida [WP_046615642.1] 0% Pseudomonas putida [WP_047933458.1] 0% 73% Pseudomonas putida [WP_075045664.1] 0% Pseudomonas putida [WP_075045665.1] 0% Pseudomonas sp. B52017 [WP_085664657.1] 0% 0%Pseudomonas sp. FGI182 [WP_025339805.1] 0%Pseudomonas sp. FGI182 [WP_025339806.1] 0%unclassified Pseudomonas [WP_085692365.1] unclassified Pseudomonas [WP_085692364.1] 91.9%Aeromonas dhakensis [WP_010634239.1] 0% Aeromonas hydrophila [QKF99360.1] Aeromonas [WP_026139045.1] 0% Aeromonas [WP_043171966.1] 93.5% 0% Aeromonas dhakensis [OBR43527.1] 0% 0%Aeromonas dhakensis [WP_082970067.1] 0%Aeromonas dhakensis [WP_095592929.1] 0%Aeromonas dhakensis [WP_124250520.1] Aeromonas dhakensis [WP_117404253.1] 67.5% 84.5% Brachyspira aalborgi [WP_147561247.1] Brachyspira pilosicoli [WP_147731173.1] Phyllobacterium sp. 628 [QND51891.1] Prevotella pallens [WP_006046273.1] 88.4% Myroides sp. CJ210 [WP_158961574.1] 44.6% 97.3% Hanstruepera neustonica [WP_123912608.1] 77.8% Hanstruepera neustonica [PNQ74664.1] 96.8% Flavobacterium sp. ICH-30 [WP_129463241.1] Flavobacterium sp. ICH-30 [WP_129463235.1] Porphyromonas macacae [WP_036873454.1] 83.8% Empedobacter tilapiae [WP_135835376.1] 85.1% Chryseobacterium carnipullorum [WP_073331451.1] 76% Chryseobacterium vrystaatense [WP_073175726.1] Chryseobacterium sp. c4a [WP_160139984.1] 44.3% Flavobacterium sp. [RZK10513.1] unclassified Sphingobacterium [WP_108633942.1] 0% Acinetobacter bereziniae [KAF1025749.1] 0% Acinetobacter bereziniae [WP_042089375.1] 0% Acinetobacter bereziniae [WP_058971211.1] 0% 0% Acinetobacter bereziniae [WP_151712790.1] 99.7% 0% Acinetobacter bereziniae [WP_151810579.1] 0% Acinetobacter bereziniae [WP_151780952.1] 0% Acinetobacter bereziniae [WP_151805745.1] 0% 77.9%90.7%Acinetobacter bereziniae [WP_100884623.1] 0% Acinetobacter bereziniae [WP_151778812.1] 0% 86.6% Acinetobacter bereziniae [WP_151797791.1] 41.6% 0%Acinetobacter bereziniae [WP_121773973.1] Acinetobacter sp. WC-743 [ELW82319.1] 85.8%Acinetobacter bereziniae [WP_139419027.1] 89.9% 0% Acinetobacter bereziniae [WP_005030825.1] 83.9%Acinetobacter bereziniae [WP_151803939.1] Acinetobacter bereziniae [WP_004828094.1] 86.7% Sphingobacterium spiritivorum [WP_115169657.1] 89.8% Elizabethkingia anophelis [WP_035592443.1] unclassified Elizabethkingia [WP_107809418.1] 0% Chryseobacterium joostei [WP_076355355.1] 60.4% Aquimarina sp. AU58 [WP_109852727.1] Chryseobacterium sp. G0201 [WP_123984125.1] Capnocytophaga canimorsus [WP_172916107.1] 74.3% Capnocytophaga canimorsus [WP_095919891.1] 78.5% 90.6%Capnocytophaga canimorsus [WP_013996611.1] 83.9% Capnocytophaga canimorsus [WP_095917687.1] 90.8% 0%Capnocytophaga canimorsus [WP_013996609.1] Capnocytophaga canimorsus [WP_095919899.1] 0%Capnocytophaga canimorsus [WP_095917685.1] 0% Capnocytophaga canimorsus [WP_044729289.1] 64.5% Capnocytophaga canimorsus [WP_126321786.1] Saezia sanguinis [WP_126980011.1] 87.5%Gilliamella apicola [WP_081300105.1] 57.8% Gilliamella apicola [OCG48415.1] 76% 0% 75.7% unclassified Apibacter [WP_160556727.1] 75.9% 84.4% unclassified Gilliamella [WP_160409290.1] 81.1% Gilliamella intestini [WP_141683103.1] 78.3%Gilliamella intestini [WP_141683083.1] 47.6% unclassified Gilliamella [WP_160407633.1] Bacteroidales [WP_117710081.1] Bacteroides sp. An19 [WP_087248849.1] 79.7%Bacteroides uniformis [WP_117681518.1] Porphyromonas macacae [WP_115096887.1] 81.6% 95.5% 0%Phocaeicola plebeius [WP_117674138.1] Phocaeicola plebeius [WP_118027884.1] Bacteroides fragilis [WP_014298491.1] 0%Bacteroides ovatus [WP_118033011.1] 85.4% Bacteroides stercoris [WP_118940230.1] 80% 0% Phocaeicola vulgatus [WP_118240864.1] 0% Bacteroides [WP_032529710.1] 73.5% 96.7% 0%Bacteroides fragilis [WP_044300576.1] 0%Bacteroides uniformis [WP_117963672.1] 81.2%0% Bacteroides intestinalis [WP_118477366.1] Bacteroidales [WP_036617852.1] 0% Bacteroides acidifaciens [GFH87176.1] 0% Bacteroides acidifaciens [WP_024986797.1] 75.3%0%0% 83.3%0%Bacteroides acidifaciens [WP_172504057.1] 0%Parabacteroides distasonis [WP_135959935.1] Parabacteroides distasonis str. 3776 D15 i [KDS37238.1] 0% Bacteroidales [WP_117775769.1] 86.6%Parabacteroides distasonis [WP_182438230.1] Parabacteroides distasonis [RHF40976.1] Bacteroides sp. CAG189 [CCY51012.1] 0% 0%Bacteroides stercoris [WP_117741663.1] 0%Bacteroides stercoris [WP_118207153.1] 0%Escherichia coli [MZW00920.1] Parabacteroides merdae [WP_117959353.1] 91.8% 83.7%Phocaeicola barnesiae [WP_018712179.1] 80.5% Bacteroides sp. OF04-15BH [WP_118526213.1] Bacteroidales [WP_005643591.1] Bacteria [WP_036632192.1] 77.7% 95.5% 33.8%0% 0%Bacteroides fragilis [KAA4966766.1] 0%Bacteroides massiliensis [EOA53330.1] 82.9% 0%Parabacteroides merdae [WP_155151151.1] 46.1% Phocaeicola massiliensis [WP_008778754.1] 0% 0%Bacteroides sp. An269 [WP_087213559.1] 0%Bacteroides sp. An279 [WP_087407122.1] Phocaeicola salanitronis [WP_013619082.1] Bacteroidales [WP_007480240.1] 0% Bacteroidales [WP_120081184.1] 0% Bacteroides caccae [WP_119216435.1] 0% Bacteroides cellulosilyticus [WP_029427647.1] 0% 79.5% Parabacteroides distasonis [WP_146376206.1] 0% 0%Parabacteroides distasonis [WP_154397794.1] 0%Parabacteroides sp. AM44-16 [WP_122379203.1] 0%Phocaeicola vulgatus [WP_005841185.1] unclassified Bacteroides [WP_033572529.1] Prevotella buccalis [WP_004349091.1] 91.5% 0%Prevotella buccalis [WP_102696650.1] 0%Prevotella corporis [WP_060940248.1] 0%Prevotella timonensis [WP_028901404.1] 75.5% Prevotella timonensis [WP_081675930.1] 95.9% Bacteroides [WP_044300581.1] 0%Bacteroides fragilis [QCQ51121.1] 82.9% Bacteroides ovatus [WP_118419264.1] Parabacteroides merdae [WP_005643594.1] 94.3% 73.2% 81.2%Bacteroidales [WP_117610801.1] 0%Bacteroidales [WP_117674209.1] 0%Bacteroides finegoldii [WP_007754579.1] Bacteroides stercoris [WP_016661997.1] Chryseobacterium vrystaatense [WP_034743871.1] 0% Chryseobacterium kwangjuense [WP_062648135.1] 84.1%99.7% Pasteurella testudinis [WP_115306408.1] 0%Pasteurella testudinis [WP_143933423.1] 0% Pasteurella testudinis [WP_115306416.1] 91.2% Sphingobacterium sp. M46 [WP_108631748.1] Sphingobacterium sp. DR205 [WP_165306743.1] 0%Roseburia sp. CAG45 [CDC13625.1] unclassified Roseburia [WP_158561827.1] 0% Roseburia sp. [HCJ75825.1] Roseburia sp. AF22-8AC [RGG38672.1] 98.1% Roseburia sp. AF20-18LB [WP_118173328.1] 0% Roseburia sp. AF12-17LB [WP_118544503.1] 0% 0%Roseburia sp. OM04-10BH [RGI42416.1] 0%0%unclassified Roseburia [WP_118087694.1] 76.4%0%unclassified Roseburia [WP_158575684.1] unclassified Roseburia [WP_158567221.1] 91.4% Blautia sp. An46 [WP_087266982.1] 85.7% Blautia sp. An81 [WP_087416516.1] 74.3% 0% 62.7% Roseburia intestinalis [WP_118592541.1] 77.4% Firmicutes bacterium CAG424 [CDC45025.1] 80.9%Lachnospiraceae bacterium [HCI24939.1] Roseburia sp. CAG10041 57 [OLA58078.1] 85.5%Clostridia bacterium [WP_178181454.1] uncultured Roseburia sp. [SCI57545.1] 80.1% Lachnospiraceae bacterium G41 [WP_089996388.1] 94% Mobiluncus holmesii [WP_154543176.1] 26.1% Mobiluncus holmesii [WP_154543164.1] Lachnospiraceae bacterium [WP_158437537.1] 73.7% Clostridia bacterium [WP_178421434.1] 90.4% Roseburia sp. AF12-17LB [WP_118665471.1] 0% Roseburia sp. AF15-21 [WP_118639184.1] 51.3% 0% Roseburia sp. AF20-18LB [WP_118173330.1] 0% Roseburia sp. AF34-16 [WP_118177153.1] 0% unclassified Roseburia [WP_117841794.1] 0% unclassified Roseburia [WP_117947697.1] 85.4% 0% 0%unclassified Roseburia [WP_118057132.1] 0%unclassified Roseburia [WP_118544506.1] 0%uncultured Roseburia sp. [SCI57442.1] Roseburia sp. CAG45 [CDC13628.1] Edaphobacillus lindanitolerans [WP_076757601.1] 80.6%Bacillus [WP_098986288.1] 0%Bacillus cereus [SCV20581.1] 78.4%0%Bacillus cereus [WP_098560669.1] 87.2% Bacillus cereus [WP_088083915.1] 77.8%79.4%Bacillus wiedmannii [WP_097859109.1] Bacillus cereus [WP_088114010.1] 0%Bacillus wiedmannii [WP_000617446.1] Bacillus wiedmannii [WP_098656078.1] 86.4% Bacillus cereus [WP_063245377.1] 86.9%Bacillus cereus [WP_088016109.1] 80.8% 0% 0% 0%Bacillus sp. ISTL8 [WP_168897802.1] 74.6% Bacillus cereus group [WP_000943014.1] Bacillus cereus [WP_061130395.1] 76.6% Bacteria [WP_033533540.1] Lysinibacillus acetophenoni [WP_097151040.1] Mobilisporobacter senegalensis [WP_170164209.1] Ruminococcus albus [SEL29808.1] 75.6%89.1%Ruminococcus albus [WP_100084590.1] 0% Ruminococcus albus [SEL29790.1] 72.5% Ruminococcus albus 8 [EGC02125.1] 30.5% Ruminococcus sp. [HCJ41952.1] 75.2% Anaeromyces robustus 95.9% Pecoramyces ruminantium 85.5% Caecomyces sp Brit4 0% Piromyces sp FS3c 0% 96.5% Anaeromyces contortus 84.9% Ruminococcus sp. AF21-11 [WP_117878620.1] 74.7% unclassified Ruminococcus [WP_117924927.1] 100% 97.5% Ruminococcus sp. AM31-15AC [WP_118158526.1] 0% 0% 0%Clostridiales [WP_117904541.1] 0%Ruminococcus sp. AM54-1NS [WP_118160537.1] unclassified Ruminococcus [WP_117898091.1] 95.2% Ruminococcus albus [WP_013496901.1] 74.7% Ruminococcus albus [WP_037288593.1] 85.1% Ruminococcus sp. [HBI85391.1] 51.6% Ruminococcus albus [WP_028504305.1] Olsenella sp. Marseille-P4559 [WP_130810245.1 94.4% Ruminococcus sp. [HBJ45436.1] 94.8% Ruminococcus sp. [HCD40066.1] Ruminococcus callidus [WP_021680325.1] Pedobacter sp. R20-19 [WP_082468447.1] 94.4% Bacteroidetes bacterium [TAF76571.1] 75.5% Emticicia sp. CRIBPO [WP_166564913.1] 86.6% 83.2% Flavobacterium sp. T132019 [WP_180907829.1] 0% Jejuia pallidilutea [WP_042244344.1] 99.5% Collinsella vaginalis [WP_085830835.1] Collinsella vaginalis [WP_179184984.1] Nostoc sp. T09 [WP_086688279.1] 99.4% 0% Calothrix sp. NIES-2100 [WP_096604092.1] 75.6% Chloroflexia bacterium SDU3-3 [WP_152297644.1] 0% 95.4% Aphanizomenon flos-aquae MDT14a [OBQ27554.1] Betaproteobacteria bacterium UKL13-2 [AMS33119.1] 90.6% Agreia pratensis [WP_085484601.1] 87% Leucobacter sp. HDW9B [WP_166323809.1] Microlunatus sp. Gsoil 973 [WP_154689302.1] Acinetobacter sp. Marseille-Q1623 [WP_174494064.1] 100% unclassified Acinetobacter [WP_180135303.1] 75.4% 0%unclassified Acinetobacter [WP_180065545.1] 0%unclassified Acinetobacter [WP_180010880.1] 0%unclassified Acinetobacter [WP_180009469.1] unclassified Acinetobacter [WP_180005255.1] 8.6% Hymenobacter terrenus [WP_046246014.1] Hymenobacter sp. [RZK22013.1]

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