CHRISTINA V. THEODORIS

EDUCATION

Postdoctoral Research Fellow, X Shirley Liu Lab Present Department of Data Science, Dana-Farber Cancer Institute (DFCI)

Clinical Fellow, Pediatrics-Medical Genetics Residency Program Present Boston Children’s Hospital, Boston Medical Center, Harvard Medical School

MD/PhD, University of , (UCSF) 2017 Developmental and Stem Cell Biology Graduate Program, GPA: 4.0

B.S. Biology, California Institute of Technology (CIT), GPA: 4.0 2009

RESEARCH EXPERIENCE

Objective: Determining the circuitry of gene regulatory networks and their disruption in cardiac disease to identify promising targets for network-correcting therapies.

December 2020 – Present X Shirley Liu Lab, DFCI Boston, MA Postdoctoral researcher n Developing a machine learning approach to embed genes into a multi-dimensional space using large amounts of publicly available transcriptional data to predict gene function and interactions

n Techniques: bioinformatics, machine learning, network inference

August 2011 – June 2017 Deepak Srivastava Lab, , UCSF San Francisco, CA MD/PhD candidate researcher n Harnessed human induced pluripotent stem cell (iPSC)-based disease modeling to reveal the transcriptional and epigenetic mechanisms underlying gene network dysregulation and disruption of endothelial shear stress response caused by NOTCH1 haploinsufficiency in calcific aortic valve disease (CAVD)

n Developed a network-based small molecule screen in the above iPSC-based disease model using a machine learning approach to identify a promising gene network-correcting therapy for CAVD

n Determined that long telomeres protect mice from age-dependent cardiac disease caused by Notch1 haploinsufficiency and that telomere shortening dysregulates genes that physically interact with telomeres, suggesting a potential mechanism for telomere-dependent regulation of homeostatic gene expression

n Techniques: bioinformatics, network inference, TALEN genome engineering, iPSC reprogramming & differentiation, ChIP-seq, RNA-seq, Meth-seq, small molecule screening, in-situ co-IP, EMSA, etc.

February 2006 – June 2009 Eric H. Davidson Lab, CIT Pasadena, CA Undergraduate Researcher n Determined kinetics of wnt8/blimp1 subcircuit driving a spatially dynamic pattern of gene expression in early sea urchin (S. pupuratus) development and defined interactions of endomesoderm patterning genes

n Elucidated the upstream regulation governing hox11/13b gene expression by cis-regulatory analysis

n Techniques: microinjection, sea urchin embryo culturing, design/creation of reporter constructs, etc.

WWW.CHRISTINATHEODORIS.COM • [email protected]

SCIENTIFIC PUBLICATIONS

C V Theodoris, P Zhou, L Liu, Y Zhang, T Nishino, Y Huang, A Kostina, S S Ranade, C A Gifford, V Uspensky, A Malashicheva, S Ding, D Srivastava. Network-based screen in iPSC-derived cells reveals therapeutic candidate for heart valve disease. Science, 12 Feb 2021.

C V Theodoris, F Mourkioti, Y Huang, S S Ranade, L Liu, H M Blau, D Srivastava. Long Telomeres Protect Against Age-Dependent Cardiac Disease Caused by NOTCH1 Haploinsufficiency. Journal of Clinical Investigation, 20 March 2017.

E Barruet, B Morales, W Lwin, M P White, C V Theodoris, H Kim, A Urrutia, S Wong, D Srivastava, E Hsiao. The human ACVR1 R206H mutation found in fibrodysplasia ossificans progressive increases human induced pluripotent stem cell-derived endothelial cell formation and collagen production through BMP-mediated SMAD1/5/8 signaling. Stem Cell Research and Therapy, 17 August 2016.

M P White, C V Theodoris, L Liu, W J Collins, K W Blue, J H Lee, X Meng, R C Robbins, K N Ivey, D Srivastava. NOTCH1 Regulates Matrix Gla Protein and Calcification Gene Networks in Human Valve Endothel