DOCSLIB.ORG

Osaro Erhabor

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Osaro Erhabor TRANSFUSION MEDICINE MADE EASY FOR STUDENTS OF ALLIED MEDICAL SCIENCES AND MEDICINE Authored by Osaro Erhabor and Teddy Charles Adias Transfusion Medicine Made Easy for Students of Allied Medical Sciences and Medicine Authored by: Dr Osaro Erhabor (Ph.D, CSci, FIBMS) and Dr Teddy Charles Adias (Ph.D, FIBMS) Edition 2014 Published by InTech Janeza Trdine 9, 51000 Rijeka, Croatia Copyright © 2012 InTech All chapters are distributed under the Creative Commons Attribution 3.0 license, which allows users to download, copy and build upon published articles even for commercial purposes, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. After this work has been published by InTech, authors have the right to republish it, in whole or part, in any publication of which they are the author, and to make other personal use of the work. Any republication, referencing or personal use of the work must explicitly identify the original source. Notice Statements and opinions expressed in the chapters are these of the individual contributors and not necessarily those of the editors or publisher. No responsibility is accepted for the accuracy of information contained in the published chapters. The publisher assumes no responsibility for any damage or injury to persons or property arising out of the use of any materials, instructions, methods or ideas contained in the book. Publishing Process Manager Silvia Vlase Typesetting Vladimir Sijan Cover InTech Design Team Additional hard copies can be obtained from [email protected] Transfusion Medicine Made Easy for Students of Allied Medical Sciences and Medicine Authored by: Dr Osaro Erhabor (Ph.D, CSci, FIBMS) and Dr Teddy Charles Adias (Ph.D, FIBMS) p. cm. ISBN 978-953-51-0523-7 We are IntechOpen, the world’s leading publisher of Open Access books Built by scientists, for scientists 3,800+ 116,000+ 120M+ Open access books available International authors and editors Downloads Our authors are among the 151 Top 1% 12.2% Countries delivered to most cited scientists Contributors from top 500 universities Selection of our books indexed in the Book Citation Index in Web of Science™ Core Collection (BKCI) Interested in publishing with us? Contact [email protected] Numbers displayed above are based on latest data collected. For more information visit www.intechopen.com Meet the authors Dr Osaro Erhabor is an Immuno-Haematologist, a Chartered Scientist, Registration Portfolio Verifier and a Fellow of the Institute of Biomedical Medical Science, London. He is a seasoned Biomedical Scientist and Lec- turer. He holds a Ph.D in Immuno-Haematology. He has taught best practices in Transfusion Medicine for several years to students in both the United Kingdom and Nige- ria. A recipient of several scientific awards, member of the editorial boards, an article reviewer to several scientific journals, a well published author and speaker in several international scientific conferences. His current research interest includes transfusion safety and alternatives and haema- tology of infectious diseases. Dr Teddy Charles Adias is currently the Provost of the Bayelsa State College of Health Technology, Og- bia-Town, Nigeria. He holds a Ph.D in Immuno-Haema- tology, as well as a Fellow of the Institute of Biomedical Science (FIBMS), London. He had held several Adjunct academic appointments with various Nigerian univer- sities and had taught at both post graduate and under- graduate levels for over nine years. His current research interest is focus on Transfusion immunology, safety and alternatives, and haematology of infectious diseases. Recent publications have included articles in Journals such as the Journal of Blood Medicine; Transfusion Clinque et Biologique, Pathology and Laboratory Medicine International amongst others. Contents Acknowledgements 1 1. History of Blood Transfusion 2 2. Antigen and Antibody 4 3. Blood Group Systems and ABO groups 20 4. Anticoagulation and Preservation in Transfusion 49 5. Blood Donation Testing 54 6. Apheresis Principle and Practice 59 7. Blood Component Preparation 60 8. Challenges of Blood Transfusion in Africa 66 9. Blood Donation and Donor Types 68 10. Advantages of Autologous Blood over Allogeneic Blood 72 11. Transfusion Transmissible Infectious Diseases 79 12. Complications of Blood Transfusion 83 13. Investigation of Blood Transfusion Reactions 90 14. Compatibility Testing 92 15. Red Blood Cells Alloimmunisation 100 16. HDFN and Management of Rh Negative Pregnancies 115 17. Transfusion Alternatives and Exemplary Stewardship in the Management of Blood and Blood Product 128 18. Blood Components Therapy 133 19. Management of Major Haemorrhage 143 20. Storage Conditions, Shelf Life Indication and Mode of Transfusion 147 22. Fractionated Plasma Products 158 23. Rhesus Blood Group System 162 24. Lewis Blood Group System 177 25. MNS Blood Group System 181 26. Kell Blood Group System 184 27. Duffy Blood Group System 186 28. Kidd Blood Group System 189 29. Bg Antibodies 190 32. Lutheran Blood Group System 194 33. Minor Blood Group Systems 194 34. Complement 196 35. The Antiglobulin Test 203 Blood transfusion is a field where there has been, and continue to be, significant advances 36. Good Manufacturing Practice (GMP) 217 in science, technology and most particularly governance. The aim of this book is to provide 37. Principle of Good Laboratory Practice (GLP) and Its Application in students of allied medical sciences, medicine and transfusion practitioners with a compre Transfusion 223 hensive overview of both the scientific and managerial aspects of blood transfusion. The book is intended to equip biomedical, clinical and allied medical professionals with practical tools 38. Quality Issues in Transfusion Medicine 230 to allow for an informed practice in the field of blood transfusion management. 247 39. Management Review Meetings in the Transfusion Laboratory Dr Erhabor Osaro 40. Standard Operating Procedure 249 41. Incident Reporting Procedure in Transfusion 255 42. Laboratory Techniques and Transfusion Sample Requirements 260 275 43. Principle of Informed Consent in Transfusion Medicine The authors are indebted to Prof E.K Uko and Prof E.A Usanga both of the Haematology and 44. Stem Cell Transplantation 279 blood transfusion Department of the University of Calabar in Nigeria for taking time out to review this book. We are also grateful to the publishers InTech. Our sincere thanks goes to 45. Alkaline Denaturation Test 289 members of our families and friend for the encouragement while we put this material that will improve the quality of transfusion medicine training and by extension transfusion serv 291 About the authors ice delivery particularly in Africa. We are eternally grateful to God for this opportunity to in our own little way improve the quality of transfusion medicine training offered to students of biomedical, medical and allied medical sciences. To God alone be all the glory. © 2012 Stopforth et al.; licensee InTech. This is an open access chapter distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Transfusion Medicine Made Easy for Students of Allied Medical Sciences and Medicine Dr Erhabor Osaro (Ph.D, CSci, FIBMS) Dr Adias Teddy Charles (Ph.D, FIBMS) Blood Sciences Department Royal Bolton Hospital UK Preface Blood transfusion is a field where there has been, and continue to be, significant advances in science, technology and most particularly governance. The aim of this book is to provide students of allied medical sciences, medicine and transfusion practitioners with a compre- hensive overview of both the scientific and managerial aspects of blood transfusion. The book is intended to equip biomedical, clinical and allied medical professionals with practical tools to allow for an informed practice in the field of blood transfusion management. Dr Erhabor Osaro Acknowledgements The authors are indebted to Prof E.K Uko and Prof E.A Usanga both of the Haematology and blood transfusion Department of the University of Calabar in Nigeria for taking time out to review this book. We are also grateful to the publishers InTech. Our sincere thanks goes to members of our families and friend for the encouragement while we put this material that will improve the quality of transfusion medicine training and by extension transfusion serv- ice delivery particularly in Africa. We are eternally grateful to God for this opportunity to in our own little way improve the quality of transfusion medicine training offered to students of biomedical, medical and allied medical sciences. To God alone be all the glory. © 2012© 2012 Erhabor Stopforth and etAdias, al.; licensee licensee InTech. InTech. This This isis ana paper open distributedaccess chapter under distributed the terms under of the the Creative terms Commons of the AttributionCreative LicenseCommons (http://creativecommons.org/licenses/by/3.0), Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted which use, permits distribution,unrestricted and use, reproduction distribution, in anyand medium,reproduction provided in any the medium, original provided work is properly the original cited. work is properly cited. 2 Dr Osaro Erhabor (Ph.D, CSci, FIBMS) and Dr Teddy Charles Adias (Ph.D, FIBMS) Transfusion Medicine
Load more

Recommended publications
  • Human and Mouse CD Marker Handbook Human and Mouse CD Marker Key Markers - Human Key Markers - Mouse
    Welcome to More Choice CD Marker Handbook For more information, please visit: Human bdbiosciences.com/eu/go/humancdmarkers Mouse bdbiosciences.com/eu/go/mousecdmarkers Human and Mouse CD Marker Handbook Human and Mouse CD Marker Key Markers - Human Key Markers - Mouse CD3 CD3 CD (cluster of differentiation) molecules are cell surface markers T Cell CD4 CD4 useful for the identification and characterization of leukocytes. The CD CD8 CD8 nomenclature was developed and is maintained through the HLDA (Human Leukocyte Differentiation Antigens) workshop started in 1982. CD45R/B220 CD19 CD19 The goal is to provide standardization of monoclonal antibodies to B Cell CD20 CD22 (B cell activation marker) human antigens across laboratories. To characterize or “workshop” the antibodies, multiple laboratories carry out blind analyses of antibodies. These results independently validate antibody specificity. CD11c CD11c Dendritic Cell CD123 CD123 While the CD nomenclature has been developed for use with human antigens, it is applied to corresponding mouse antigens as well as antigens from other species. However, the mouse and other species NK Cell CD56 CD335 (NKp46) antibodies are not tested by HLDA. Human CD markers were reviewed by the HLDA. New CD markers Stem Cell/ CD34 CD34 were established at the HLDA9 meeting held in Barcelona in 2010. For Precursor hematopoetic stem cell only hematopoetic stem cell only additional information and CD markers please visit www.hcdm.org. Macrophage/ CD14 CD11b/ Mac-1 Monocyte CD33 Ly-71 (F4/80) CD66b Granulocyte CD66b Gr-1/Ly6G Ly6C CD41 CD41 CD61 (Integrin b3) CD61 Platelet CD9 CD62 CD62P (activated platelets) CD235a CD235a Erythrocyte Ter-119 CD146 MECA-32 CD106 CD146 Endothelial Cell CD31 CD62E (activated endothelial cells) Epithelial Cell CD236 CD326 (EPCAM1) For Research Use Only.
    [Show full text]
  • The Membrane Complement Regulatory Protein CD59 and Its Association with Rheumatoid Arthritis and Systemic Lupus Erythematosus
    Current Medicine Research and Practice 9 (2019) 182e188 Contents lists available at ScienceDirect Current Medicine Research and Practice journal homepage: www.elsevier.com/locate/cmrp Review Article The membrane complement regulatory protein CD59 and its association with rheumatoid arthritis and systemic lupus erythematosus * Nibhriti Das a, Devyani Anand a, Bintili Biswas b, Deepa Kumari c, Monika Gandhi c, a Department of Biochemistry, All India Institute of Medical Sciences, New Delhi 110029, India b Department of Zoology, Ramjas College, University of Delhi, India c University School of Biotechnology, Guru Gobind Singh Indraprastha University, India article info abstract Article history: The complement cascade consisting of about 50 soluble and cell surface proteins is activated in auto- Received 8 May 2019 immune inflammatory disorders. This contributes to the pathological manifestations in these diseases. In Accepted 30 July 2019 normal health, the soluble and membrane complement regulatory proteins protect the host against Available online 5 August 2019 complement-mediated self-tissue injury by controlling the extent of complement activation within the desired limits for the host's benefit. CD59 is a membrane complement regulatory protein that inhibits the Keywords: formation of the terminal complement complex or membrane attack complex (C5b6789n) which is CD59 generated on complement activation by any of the three pathways, namely, the classical, alternative, and RA SLE the mannose-binding lectin pathway. Animal experiments and human studies have suggested impor- Pathophysiology tance of membrane complement proteins including CD59 in the pathophysiology of rheumatoid arthritis Disease marker (RA) and systemic lupus erythematosus (SLE). Here is a brief review on CD59 and its distribution, structure, functions, and association with RA and SLE starting with a brief introduction on the com- plement system, its activation, the biological functions, and relations of membrane complement regu- latory proteins, especially CD59, with RA and SLE.
    [Show full text]
  • Mcleod Neuroacanthocytosis Syndrome
    NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health. Pagon RA, Adam MP, Ardinger HH, et al., editors. GeneReviews® [Internet]. Seattle (WA): University of Washington, Seattle; 1993- 2017. McLeod Neuroacanthocytosis Syndrome Hans H Jung, MD Department of Neurology University Hospital Zurich Zurich, Switzerland [email protected] Adrian Danek, MD Neurologische Klinik Ludwig-Maximilians-Universität München, Germany ed.uml@kenad Ruth H Walker, MD, MBBS, PhD Department of Neurology Veterans Affairs Medical Center Bronx, New York [email protected] Beat M Frey, MD Blood Transfusion Service Swiss Red Cross Schlieren/Zürich, Switzerland [email protected] Christoph Gassner, PhD Blood Transfusion Service Swiss Red Cross Schlieren/Zürich, Switzerland [email protected] Initial Posting: December 3, 2004; Last Update: May 17, 2012. Summary Clinical characteristics. McLeod neuroacanthocytosis syndrome (designated as MLS throughout this review) is a multisystem disorder with central nervous system (CNS), neuromuscular, and hematologic manifestations in males. CNS manifestations are a neurodegenerative basal ganglia disease including (1) movement disorders, (2) cognitive alterations, and (3) psychiatric symptoms. Neuromuscular manifestations include a (mostly subclinical) sensorimotor axonopathy and muscle weakness or atrophy of different degrees. Hematologically, MLS is defined as a specific blood group phenotype (named after the first proband, Hugh McLeod) that results from absent expression of the Kx erythrocyte antigen and weakened expression of Kell blood group antigens. The hematologic manifestations are red blood cell acanthocytosis and compensated hemolysis. Allo-antibodies in the Kell and Kx blood group system can cause strong reactions to transfusions of incompatible blood and severe anemia in newborns of Kell-negative mothers.
    [Show full text]
  • Newsletteralumni News of the Newyork-Presbyterian Hospital/Columbia University Department of Surgery Volume 13, Number 1 Summer 2010
    NEWSLETTERAlumni News of the NewYork-Presbyterian Hospital/Columbia University Department of Surgery Volume 13, Number 1 Summer 2010 CUMC 2007-2009 Transplant Activity Profile* Activity Kidney Liver Heart Lung Pancreas Baseline list at year start 694 274 174 136 24 Deceased donor transplant 123 124 93 57 11 Living donor transplant 138 17 — 0 — Transplant rate from list 33% 50% 51% 57% 35% Mortality rate while on list 9% 9% 9% 15% 0% New listings 411 217 144 68 23 Wait list at year finish 735 305 204 53 36 2007-June 2008 Percent 1-Year Survival No % No % No % No % No % Adult grafts 610 91 279 86 169 84 123 89 6 100 Adult patients 517 96 262 88 159 84 116 91 5 100 Pediatric grafts 13 100 38 86 51 91 3 100 0 — Pediatric patients 11 100 34 97 47 90 2 100 0 — Summary Data Total 2009 living donor transplants 155 (89% Kidney) Total 2009 deceased donor transplants 408 (30% Kidney, 30% Liver) 2007-June 2008 adult 1-year patient survival range 84% Heart to 100% Pancreas 2007-June 2008 pediatric 1-year patient survival range 90% Heart to 100% Kidney or lung *Health Resource and Service Administration’s Scientific Registry of Transplant Recipients (SRTR) Ed Note. The figure shows the US waiting list for whole organs which will only be partially fulfilled by some 8,000 deceased donors, along with 6,600 living donors, who will provide 28,000 to 29,000 organs in 2010. The Medical Center’s role in this process is summarized in the table, and the articles that follow my note expand on this incredible short fall and its potential solutions.
    [Show full text]
  • Immuno 2014 No. 1
    Journal of Blood Group Serology and Molecular Genetics VOLUME 30, N UMBER 1, 2014 Immunohematology Journal of Blood Group Serology and Molecular Genetics Volume 30, Number 1, 2014 CONTENTS R EPORT 1 Indirect antiglobulin test-crossmatch using low-ionic-strength saline–albumin enhancement medium and reduced incubation time: effectiveness in the detection of most clinically significant antibodies and impact on blood utilization C.L. Dinardo, S.L. Bonifácio, and A. Mendrone, Jr. R EV I EW 6 Raph blood group system M. Hayes R EPORT 11 I-int phenotype among three individuals of a Parsi community from Mumbai, India S.R. Joshi C A SE R EPORT 14 Evans syndrome in a pediatric liver transplant recipient with an autoantibody with apparent specificity for the KEL4 (Kpb) antigen S.A. Koepsell, K. Burright-Hittner, and J.D. Landmark R EV I EW 18 JMH blood group system: a review S.T. Johnson R EPORT 24 Demonstration of IgG subclass (IgG1 and IgG3) in patients with positive direct antiglobulin tests A. Singh, A. Solanki, and R. Chaudhary I N M EMOR ia M 28 George Garratty, 1935–2014 Patricia A. Arndt and Regina M. Leger 30 A NNOUNCEMENTS 34 A DVERT I SEMENTS 39 I NSTRUCT I ONS FOR A UTHORS E D I TOR - I N -C H I EF E D I TOR ia L B OA RD Sandra Nance, MS, MT(ASCP)SBB Philadelphia, Pennsylvania Patricia Arndt, MT(ASCP)SBB Paul M. Ness, MD Pomona, California Baltimore, Maryland M A N AG I NG E D I TOR James P.
    [Show full text]
  • 3407 M16141436 19 3.Pdf
    Immunohematology JOURNAL OF BLOOD GROUP SEROLOGY AND EDUCATION V OLUME 19, NUMBER 3, 2003 Immunohematology JOURNAL OF BLOOD GROUP SEROLOGY AND EDUCATION V OLUME 19, NUMBER 3, 2003 CONTENTS 73 DNA analysis for donor screening of Dombrock blood group antigens J.R. STORRY, C.M.WESTHOFF,D.CHARLES-PIERRE,M.RIOS,K.HUE-ROYE,S.VEGE,S.NANCE, AND M.E. REID 77 Studies on the Dombrock blood group system in non-human primates C. MOGOS,A.SCHAWALDER,G.R. HALVERSON, AND M.E. REID 83 Murine monoclonal antibodies can be used to type RBCs with a positive DAT G.R. HALVERSON,P.HOWARD,H.MALYSKA,E.TOSSAS, AND M.E. REID 86 Rh antigen and phenotype frequencies and probable genotypes for the four main ethnic groups in Port Harcourt, Nigeria Z.A. JEREMIAH AND F.I. BUSERI 89 Antibodies detected in samples from 21,730 pregnant women S. JOVANOVIC-SRZENTIC,M.DJOKIC,N.TIJANIC,R.DJORDJEVIC,N.RIZVAN,D.PLECAS, AND D. FILIMONOVIC 93 BOOK REVIEWS S. GERALD SANDLER,MD THERESA NESTER,MD 95 COMMUNICATIONS Letter to the Editors Letter From the Editors Irregular RBC antibodies in the Ortho Dedication sera of Brazilian pregnant women 97 IN MEMORIAM BERTIL CEDEGREN,MD 98 99 ANNOUNCEMENTS ADVERTISEMENTS 103 INSTRUCTIONS FOR AUTHORS EDITOR-IN-CHIEF MANAGING EDITOR Delores Mallory, MT(ASCP)SBB Mary H. McGinniss,AB, (ASCP)SBB Rockville, Maryland Bethesda, Maryland TECHNICAL EDITOR SENIOR MEDICAL EDITOR Christine Lomas-Francis, MSc Scott Murphy, MD New York, New York Philadelphia, Pennsylvania ASSOCIATE MEDICAL EDITORS S. Gerald Sandler, MD Geralyn Meny, MD Ralph Vassallo, MD Washington, District of Columbia Philadelphia, Pennsylvania Philadelphia, Pennsylvania EDITORIAL BOARD Patricia Arndt, MT(ASCP)SBB W.
    [Show full text]
  • ABO, Rh and Kell) and Ncovid-19 Susceptibility – a Retrospective Observational Study
    Relationship between blood group phenotypes (ABO, Rh and Kell) and nCOVID-19 susceptibility – A retrospective observational study. Sudhir Bhandari SMS Medical College and Hospitals, Jaipur, Rajasthan, India Ajeet Singh Shaktawat SMS Medical College and Hospitals, Jaipur, Rajasthan, India Amit Tak ( [email protected] ) SMS Medical College and Hospitals, Jaipur, Rajasthan, India https://orcid.org/0000-0003-2509-2311 Bhoopendra Patel Government Medical College, Barmer, Rajasthan, India Jyotsna Shukla SMS Medical College and Hospitals, Jaipur, Rajasthan, India Sanjay Singhal SMS Medical College and Hospitals, Jaipur, Rajasthan, India Kapil Gupta SMS Medical College and Hospitals, Jaipur, Rajasthan, India Jitendra Gupta SMS Medical College and Hospitals, Jaipur, Rajasthan, India Shivankan Kakkar SMS Medical College and Hospitals, Jaipur, Rajasthan, India Amitabh Dube SMS Medical College and Hospitals, Jaipur, Rajasthan, India Sunita Dia Medstar Washington Hospital Center, Washington DC 20010, USA. Mahendra Dia North Carolina State University, Raleigh, NC 27695-7609, USA. Todd C Wehner North Carolina State University, Raleigh, NC 27695-7609, USA. Research Article Keywords: ABO blood grouping, coronavirus disease, COVID-19, multinomial test Page 1/13 Posted Date: July 10th, 2020 DOI: https://doi.org/10.21203/rs.3.rs-39611/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 2/13 Abstract Since the outbreak of coronavirus disease-19 research has been continued to explore multiple facets of the disease. The objective of the present study is to evaluate the relationship between blood group phenotypes and COVID-19 susceptibility. In this hospital based, retrospective observational study 132 COVID-19 patients were enrolled from SMS Medical College and attached Hospitals, Jaipur, India after the proper approval from the institutional ethics committee.
    [Show full text]
  • ABH Secretor and Lewis Subtype Status Peter J
    Metabolic and Immunologic Consequences of ABH Secretor and Lewis Subtype Status Peter J. D’Adamo, ND, and Gregory S. Kelly, ND Abstract Determining ABH secretor phenotype and/or Lewis (Le) blood group status can be useful to the metabolically-oriented clinician. For example, differences in ABH secretor status drastically alter the carbohydrates present in body fluids and secretions; this can have profound influence on microbial attachment and persistence. Lewis typing is one genetic marker which might help identify subpopulations of individuals genetically prone to insulin resistance, autoimmunity, and heart disease. Understanding the clinical significance of ABH secretor status and the Lewis blood groups can provide insight into seemingly unrelated aspects of physiology, including variations in intestinal alkaline phosphatase activity, propensities toward blood clotting, reliability of some tumor markers, the composition of breast milk, and several generalized aspects of the immune function. Since the relevance of ABH blood group antigens as tumor markers and parasitic/bacterial/viral receptors and their association with immunologically important proteins is now well established, the prime biologic role for ABH blood group antigens may well be independent and unrelated to the erythrocyte. (Altern Med Rev 2001;6(4):390-405) Functional and Genetic Factors Involved in ABH Secretion The term “ABH secretor,” as used in blood banking, refers to secretion of ABO blood group antigens in fluids such as saliva, sweat, tears, semen, and serum. A person who is an ABH secretor will secrete antigens according to their blood group; for example, a group O individual will secrete H antigen, a group A individual will secrete A and H antigens, etc.
    [Show full text]
  • Hearts with Cardiac Arrest History Safe for Transplant in the Context Of
    Yale University EliScholar – A Digital Platform for Scholarly Publishing at Yale Yale Medicine Thesis Digital Library School of Medicine January 2015 Hearts With Cardiac Arrest History Safe For Transplant In The onC text Of Donor And Recipient Factors Aditi Balakrishna Yale School of Medicine, [email protected] Follow this and additional works at: http://elischolar.library.yale.edu/ymtdl Recommended Citation Balakrishna, Aditi, "Hearts With Cardiac Arrest History Safe For Transplant In The onC text Of Donor And Recipient Factors" (2015). Yale Medicine Thesis Digital Library. 1945. http://elischolar.library.yale.edu/ymtdl/1945 This Open Access Thesis is brought to you for free and open access by the School of Medicine at EliScholar – A Digital Platform for Scholarly Publishing at Yale. It has been accepted for inclusion in Yale Medicine Thesis Digital Library by an authorized administrator of EliScholar – A Digital Platform for Scholarly Publishing at Yale. For more information, please contact [email protected]. Hearts With Cardiac Arrest History Safe For Transplant in the Context Of Donor And Recipient Factors A Thesis Submitted to the Yale University School of Medicine in Partial Fulfillment of the Requirements for the Degree of Doctor of Medicine by Aditi Balakrishna MD Candidate, Class of 2015 Yale School of Medicine Under the supervision of Dr. Pramod Bonde, Department of Surgery Abstract Background: Cardiac arrest, or downtime, can result in ischemic damage to myocardial tissue, which prompts caution in accepting hearts with such a history for transplant. Our aim is to provide guidance about whether these hearts are suitable and which among them confer optimal outcome.
    [Show full text]
  • New Methods for Characterization of N-Type Glycosylation of Proteins by Integration of LC-MS/MS and NMR
    New Methods for Characterization of N-type Glycosylation of Proteins by Integration of LC-MS/MS and NMR Thesis Submitted in fulfillment of the requirements of the degree Doctor rer. nat. at the Department of Chemistry, Faculty of Sciences, University of Hamburg by Alena Wiegandt from Hamburg Hamburg, 2016 This thesis was prepared at the Institute for Organic Chemistry from October 2012 to July 2016 (managing director: Prof. Dr. C.B.W. Stark). I would like to thank Prof. Dr. Bernd Meyer for his continuous and motivating support during the work on my PhD thesis. I would like to thank Prof. Dr. Dr. h. c. mult. Wittko Francke for being the second reviewer of this thesis. 1st Reviewer: Prof. Dr. Bernd Meyer 2nd Reviewer: Prof. Dr. Dr. h.c. mult. Wittko Francke Date of defense: 07.04.2017 OUTLINE Outline ABBREVIATIONS ..................................................................................................................... VIII 1 SUMMARY ............................................................................................................................ 1 2 ZUSAMMENFASSUNG .......................................................................................................... 4 3 INTRODUCTION ................................................................................................................... 7 3.1 Glycosylation of Proteins - Biosynthesis, Structures, Functions .................................... 8 3.2 Glycans in Human Diseases .........................................................................................
    [Show full text]
  • Inclusion of Additional Reference Reagents in the Existing Collection of WHO International Reference Reagents for Blood Group Genotyping
    WHO/BS/2019.2371 ENGLISH ONLY EXPERT COMMITTEE ON BIOLOGICAL STANDARDIZATION Geneva, 21 to 25 October 2019 Inclusion of Additional Reference Reagents in the Existing Collection of WHO International Reference Reagents for Blood Group Genotyping Report of the international collaborative study to evaluate eighteen additional candidates for addition to the existing collection of four WHO International Reference Reagents for blood group genotyping Evgeniya Volkova1, Emilia Sippert1, Meihong Liu1, Teresita Mercado1, Gregory A Denomme2, Orieji Illoh1, Zhugong Liu1, Maria Rios1*, and the Collaborative Study Group3 1 Office of Blood Research and Review, CBER/FDA, Silver Spring, MD, USA. 2 Blood Research Institute and Diagnostic Laboratories, Versiti/BloodCenter of Wisconsin, Milwaukee, WI, USA. * Principal contact: [email protected] 3 Members of the Collaborative Study Group are listed in Appendix 1. NOTE: This document has been prepared for the purpose of inviting comments and suggestions on the proposals contained therein, which will then be considered by the Expert Committee on Biological Standardization (ECBS). Comments MUST be received by 27 September 2019 and should be addressed to the World Health Organization, 1211 Geneva 27, Switzerland, attention: Technologies, Standards and Norms (TSN). Comments may also be submitted electronically to the Responsible Officer: Dr Ivana Knezevic at email: [email protected]. © World Health Organization 2019 All rights reserved. This draft is intended for a restricted audience only, i.e. the individuals and organizations having received this draft. The draft may not be reviewed, abstracted, quoted, reproduced, transmitted, distributed, translated or adapted, in part or in whole, in any form or by any means outside these individuals and organizations (including the organizations' concerned staff and member organizations) without the permission of the World Health Organization.
    [Show full text]
  • Red Blood Cell Antigen Genotyping
    Red Blood Cell Antigen Genotyping Testing is useful in determining allelic variants predicting red blood cell (RBC) antigen phenotypes for patients with recent history of transfusion or with conflicting serological antibody results due to partial, variant, or weak expression antigens. Also Tests to Consider useful as an aid in management of hemolytic disease of the fetus and newborn (HDFN). Typical Testing Strategy Disease Overview Phenotype Testing Evaluates specific RBC antigen presence by serology Prevalence and/or Incidence Results can aid in selecting antigen negative RBC units Erythrocyte alloimmunization occurs in up to 58% of sickle cell patients, up to 35% in other transfusion-dependent patients, and in approximately 0.8% of all pregnant Antigen Testing, RBC Phenotype women. Extended 0013020 Method: Hemagglutination Serological testing includes K, Fya, Fyb, Jka, Symptoms Jkb, S, s (k, cellano, testing performed if indicated) to assess maternal or paternal RBC Transfusion reactions or HDFN can occur due to alloimmunization: phenotype status. Antigen Testing, Rh Phenotype 0013019 Intravascular hemolysis: hemoglobinuria, jaundice, shock Extravascular hemolysis: fever and chills Method: Hemagglutination HDFN: fetal hemolytic anemia, hepatosplenomegaly, jaundice, erythroblastosis, Antigen testing for D, C, E, c, and e to assess neurological damage, hydrops fetalis maternal, paternal, or newborn Rh phenotype status Clinical presentation is variable and dependent upon the specific antibody and recipient factors Genotype Testing May help
    [Show full text]