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Abstracts of the 4Th International Congress of Molecular Medicine

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Abstracts of the 4Th International Congress of Molecular Medicine in vivo 25: 467-576 (2011) ABSTRACTS OF THE 4TH INTERNATIONAL CONGRESS OF MOLECULAR MEDICINE 27-30 June, 2011 Istanbul, Turkey Under the Auspices and Support of Yeditepe University, Istanbul, Turkey The Scientific and Technological Research Council of Turkey (TÜBITAK) The Turkish Society of Molecular Medicine The International Institute of Anticancer Research, Athens, Greece Congress President Turgay İsbir, Istanbul, Turkey Honorary Presidents Nurcan Baç, Istanbul, Turkey Ülker Turgut, Istanbul, Turkey Ayça Vitrinel, Istanbul, Turkey Vice Presidents Nezih Hekim, Istanbul, Turkey Necip İlhan, Elazig, Turkey Mehmet İsbir, Antalya, Turkey İlhan Yaylım Eraltan, Istanbul, Turkey 467 Welcome to the 4th International Congress of Molecular Medicine Dear Colleagues, On behalf of the Organizing Committee, it is my great pleasure to welcome you to the 4th International Congress of Molecular Medicine. The congress has brought together recognized scientists and physicians from all around the world to discuss trends, technologies and clinical applications in Molecular Medicine. For the clinician, this is an opportunity to focus on advances in the basic sciences forming the basis of clinical practice. For the scientist, there is a perspective to appreciate the wide- reaching clinical relevance of scientific discovery. Istanbul is such a vibrant and fascinating city, ancient and modern, religious and secular, Asian and European, mystical and earthly. Therefore, we hope that during the congress you will enjoy a rich scientific program and feel the spirit of Istanbul. We encourage every participant to be active and use this occasion to exchange ideas, and build collaborations; and we hope that your stay in Istanbul will be academically, educationally and socially rewarding. On behalf of the Organizing Committee, Prof. Turgay Isbir Chairperson of the Turkish Society of Molecular Medicine Department of Medical Biology, Faculty of Medicine, Yeditepe University, Istanbul, Turkey Abstracts of the 4th International Congress of Molecular Medicine, 27-30 June, 2011, Istanbul, Turkey Congress Secretariat Hüveyda Başağa, Istanbul, Turkey Bedia Ağaçhan, Istanbul, Turkey Aslı Baykal, Antalya, Turkey Burak Dalan, Istanbul, Turkey Bülent Bayraktar, Istanbul, Turkey Arzu Ergen, Istanbul, Turkey Ahmet Belce, Istanbul, Turkey Oğuz Öztürk, Istanbul, Turkey Yusuf Özgür Çakmak, Istanbul, Turkey Hülya Yılmaz, Istanbul, Turkey Mahmut Çarin, Istanbul, Turkey Ümit Zeybek, Istanbul, Turkey Gülden Çelik, Istanbul, Turkey Triga Tourism Congress and Organization Reha Cengizler, Istanbul, Turkey Feyza Darendeliler, Istanbul, Turkey Congress Assistant Secretariat Gülnür Deniz, Istanbul, Turkey Ahmed El-Sohemy, Toronto, Canada Canan Cacına, Istanbul, Turkey Nesrin Emekli, Istanbul, Turkey Alev Cumbul, Istanbul, Turkey Özcan Erel, Ankara, Turkey Berna Demircan, Istanbul, Turkey Zehra Eren, Istanbul, Turkey E. Çiğdem Kaspar, Istanbul, Turkey Bora Farsak, Istanbul, Turkey Deniz Kıraç, Istanbul, Turkey Ece Genç, Istanbul, Turkey Özlem Küçükhüseyin, Istanbul, Turkey Mehmet Güven, Istanbul, Turkey Özlem Timirci Kahraman, Istanbul, Turkey Tuncay Hatun, Istanbul, Turkey Bahar Toptaş, Istanbul, Turkey Osman Hayran, Istanbul, Turkey Serap İnal, Istanbul, Turkey Organizing Committee Selim İsbir, Istanbul, Turkey Alper Tunga Akarsubaşı, Istanbul, Turkey Cem İyibozkurt, Istanbul, Turkey Rukset Attar, Istanbul, Turkey Güldal İzbırak, Istanbul, Turkey Makbule Aydın, Istanbul, Turkey Gülçin Kantarcı, Istanbul, Turkey Nesrin Erçelen, Istanbul, Turkey Kubilay Karşıdağ, Istanbul, Turkey Uzay Görmüş, Istanbul, Turkey Güldal Kırkali, Istanbul, Turkey Yeşim Gürol, Istanbul, Turkey Emine Kökoğlu, Istanbul, Turkey Nevin İlhan, Elazig, Turkey Ömer Küçük, Atlanta, USA Ayten Kandilci, Istanbul, Turkey Cihat Küçükhüseyin, Istanbul, Turkey Fehmi Narter, Istanbul, Turkey Mehmet Kurtoğlu, Istanbul, Turkey Tülin Öztürk, Istanbul, Turkey Akif Maharramov, Istanbul, Turkey Nilüfer Yiğit, Istanbul, Turkey Adile Muz, Elazig, Turkey Fatma Oğuz, Istanbul, Turkey International Scientific Steering Committee Uğur Özbek, Istanbul, Turkey Angelo Azzi, Boston, USA Tomris Özben, Antalya, Turkey Peter Butterworth, London, United Kingdom İnci Özden, Istanbul, Turkey John G. Delinassios, Kapandriti, Attica, Greece Ferda Özkan, Istanbul, Turkey Shant Kumar, Manchester, United Kingdom Hatice Paşaoğlu, Ankara, Turkey Franz Theuring, Berlin, Germany Fikrettin Şahin, Istanbul, Turkey Aldo Tomasi, Modena, Italy Hüseyin Sönmez, Istanbul, Turkey Asuman Sunguroğlu, Ankara, Turkey Kadirhan Sunguroğlu, Ankara, Turkey Scientific Committee Azmi Telefoncu, Izmir, Turkey Nejat Akar, Ankara, Turkey Seyhan, Tükel, Adana, Turkey Tevfik Akoğlu, Istanbul, Turkey Turgut, Ulutin, Istanbul, Turkey Figen Aksoy, Istanbul, Turkey Ünal, Uslu, Istanbul, Turkey Kemal Altaş, Istanbul, Turkey Elif, Vatanoğlu, Istanbul, Turkey Erkut Attar, Istanbul, Turkey Ayşen, Yarat, Istanbul, Turkey Filiz Aydın, Istanbul, Turkey Turay, Yardımcı, Istanbul, Turkey Canan Aykut Bingöl, Istanbul, Turkey Bayram, Yılmaz, Istanbul, Turkey Gülseren Bağcı, Denizli, Turkey Gülden, Yılmaz, Istanbul, Turkey Hüseyin Bağcı, Denizli, Turkey Güzide, Yücebilgiç, Adana, Turkey 469 in vivo 25: 467-576 (2011) Oral Presentations makes it plausible to ask whether FVL has caused selective (Alphabetically by presenter’s surname) advantages during evolution. The Turkish population seems to be a good candidate to study the possible selective disadvantages with significantly high FVL. Results: Frequency 1 was 11.5 and 8.0% in newborns (57 FVL in 494) and adults APOPTOTIC TENDENCY AND GENOMIC (357 FVL in 4,431), respectively. The difference was ABNORMALITIES OF SMOOTH MUSCLE statistically significant (p=0.015). A question arises: “Did CELLS IN THORACIC AORTIC ANEURYSMS some of the infants with FVL mutation die of clinical conditions related to thromboembolism before reaching adult Ceyda Açılan1, Müge Serhatlı1, Zelal Adıgüzel1, age and without receiving a specific diagnosis?”. This may Ömer Kaçar1, Altug Tuncer2 and Kemal Baysal1,3 explain the frequency difference between the newborns and 1Genetic Engineering and Biotechnology Institute, adults. We compared the effect of FVL in thrombotic children TÜBİTAK Marmara Research Center, Gebze, Kocaeli, with and without FVL. Kaplan–Meier analysis revealed that Turkey; FVL affects morbidity (p<0.05). Also, FVL increased the risk 2Kartal Koşuyolu Advanced Training and Research Hospital, for pediatric stroke significantly. FVL was also increased T.C. Ministry of Health, Kartal, İstanbul, Turkey; among the elderly, aged over 70 years old (29 FVL in 266 3Department of Biochemistry, Medical Faculty, Dokuz Eylül (11.5 %)). The difference between the two groups was not University, İnciraltı, Izmir, Turkey significant (p=0.8; p=0.13). Conclusion: If these data and hypothesis are verified by other studies, it will be a very Background: During the development of aortic aneurysms, important finding to explain advantageous role of FVL for degradation of extracellular matrix and loss of smooth muscle natural selection from an evolutionary point of view. cells (SMCs) through apoptosis appear to be the major factors that lead to structural deterioration, leading to progressive dilation. Reactive oxygen species (ROS) are produced well 3 above physiological levels in aneurysm tissues and are known RECOMBINANT FERROPORTIN to regulate both of these changes. Here, we hypothesized that: INFLUENCES THE PARASITE LOAD AND (i) SMCs are more susceptible to apoptosis, (ii) at least some IMMUNE RESPONSES IN BALB/C MICE cells undergo apoptosis in response to elevated ROS in the INFECTED WITH LEISHMANIA MAJOR aortic wall and (iii) p53 may be an important player in ROS- Mohammad Hossein Alimohammadian1, Aras Rafiei2, induced apoptosis. Methods: Cell death in response to H O 2 2 Seyed Mohammad Reza Fatemi2, Farhad Riazi-Rad1, was measured by WST-1 assay and apoptosis was confirmed Haiedeh Darabi1, Vahid Khazee1 and Soheila Ajdary1 by AnnexinV/PI, DNA condensation and live-cell microscopy. DNA damage was measured by micronuclei frequency and 1Pasteur Institute of Iran, Tehran, Iran; compared to the percentage of binucleate cells, age, gender, 2Department of Marine Biology, Sciences and Research hypertension or aortic diameter. The role of p53 was tested by Branch, Islamic Azad University, Hesarak, Tehran, Iran siRNA silencing. Results: While the tendency for apoptosis did not appear to be significantly different when compared to Inoculation of inbred mice by Leishmania major results in two normal cells, the percentage of micronuclei was higher in distinct patterns. In contrast to resistant mice, BALB/c mice aneurismal SMCs. Moreover, cell death was unchanged when show susceptibility to Leishmania major with visceral p53 was reduced. Conclusion: Apoptosis of SMCs can still manifestation, anemia and death. The purpose of this study take place in the absence of p53 and there is increased DNA was to express the recombinant ferroportin (rFpn), a major damage in aneurysm samples. regulator of iron homeostasis, in enterocytes of L. major- infected BALB/c mice. cDNA was prepared from total RNA of ferroportin (Fpn) extracted from zebrafish duodenum. The 2 PCR products were cloned in Topo TA vector and sub-cloned FACTOR V LEIDEN AND NATURAL SELECTION into pEGFP–N1vector. The resulted plasmid (pEGFP-ZFpn) encapsulated in chitosan/alginate nanoparticles was Nejat Akar administered orally to L. major-infected BALB/c mice. TOBB-ETU Hospital, Ankara, Turkey Treated and untreated mice were analyzed
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