SANTA CRUZ BIOTECHNOLOGY, INC. cathepsin D (D-7): sc-377299

BACKGROUND APPLICATIONS The cathepsin family of proteolytic enzymes contains several diverse classes cathepsin D (D-7) is recommended for detection of cathepsin D of mouse, rat of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S, and human origin by Western Blotting (starting dilution 1:100, dilution range and O. The aspartyl protease class is composed of cathepsins D and E. 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein Cathepsin G is in the serine protease class. Most cathepsins are lysosomal (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution and each is involved in cellular metabolism, participating in various events range 1:50-1:500), immunohistochemistry (including paraffin-embedded such as peptide biosynthesis and protein degradation. Cathepsins may also sections) (starting dilution 1:50, dilution range 1:50-1:500) and solid phase cleave some protein precursors, thereby releasing regulatory peptides. The ELISA (starting dilution 1:30, dilution range 1:30-1:3000). promoter region of the cathepsin D gene contains five Sp1 binding sites and Suitable for use as control antibody for cathepsin D siRNA (h): sc-29239, four AP-2 binding sites. cathepsin D siRNA (m): sc-29934, cathepsin D siRNA (r): sc-270475, cathepsin D shRNA Plasmid (h): sc-29239-SH, cathepsin D shRNA REFERENCES Plasmid (m): sc-29934-SH, cathepsin D shRNA Plasmid (r): sc-270475-SH, 1. Ishidoh, K., et al. 1987. Molecular cloning and sequencing of cDNA for rat cathepsin D shRNA (h) Lentiviral Particles: sc-29239-V, cathepsin D cathepsin L. FEBS Lett. 223: 69-73. shRNA (m) Lentiviral Particles: sc-29934-V and cathepsin D shRNA (r) 2. Ishidoh, K., et al. 1987. Molecular cloning and sequencing of cDNA for rat Lentiviral Particles: sc-270475-V. cathepsin H. Homology in pro-peptide regions of cysteine proteases. FEBS Molecular Weight of immature cathepsin D: 52 kDa. Lett. 226: 33-37. Molecular Weight of intermediate cathepsin D: 46 kDa. 3. Redecker, B., et al. 1991. Molecular organization of the human cathepsin D Molecular Weight of mature cathepsin D: 33 kDa. gene. DNA Cell Biol. 10: 423-431. Positive Controls: Ramos cell lysate: sc-2216, RAW 264.7 whole cell lysate: 4. Shi, G.P., et al. 1992. Molecular cloning and expression of human alveolar sc-2211 or NIH/3T3 whole cell lysate: sc-2210. macrophage cathepsin S, an elastinolytic cysteine protease. J. Biol. Chem. 267: 7258-7262. DATA CHROMOSOMAL LOCATION A B ABC D Genetic locus: CTSD (human) mapping to 11p15.5; Ctsd (mouse) mapping to 7 F5. 114 K – 85 K – SOURCE cathepsin D (D-7) is a mouse monoclonal antibody specific for an epitope 49 K – < cathepsin D mapping between amino acids 383-411 at the C-terminus of cathepsin D of human origin. cathepsin D (D-7) Alexa Fluor® 647: sc-377299 AF647. cathepsin D (D-7): sc-377299. Immunofluorescence Direct fluorescent western blot analysis of cathepsin D staining of methanol-fixed HeLa cells showing cyto- PRODUCT expression in RAW 264.7 (A), NIH/3T3 (B), 3T3-L1 (C) plasmic vesicles localization (A). Immunoperoxidase and Ramos (D) whole cell lysates. Blocked with staining of formalin fixed, paraffin-embedded human Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% UltraCruz® Blocking Reagent: sc-516214. salivary gland tissue showing cytoplasmic staining of sodium azide and 0.1% gelatin. glandular cells (B). cathepsin D (D-7) is available conjugated to agarose (sc-377299 AC), 500 µg/ SELECT PRODUCT CITATIONS 0.25 ml agarose in 1 ml, for IP; to HRP (sc-377299 HRP), 200 µg/ml, for WB, 1. Liu, H., et al. 2015. Inhibition of autophagy recovers cardiac dysfunction IHC(P) and ELISA; and to either phycoerythrin (sc-377299 PE), fluorescein and atrophy in response to tail-suspension. Life Sci. 121: 1-9. (sc-377299 FITC) or Alexa Fluor® 488 (sc-377299 AF488) or Alexa Fluor® 647 (sc-377299 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM. 2. Huang, J., et al. 2019. Exercise activates lysosomal function in the brain through AMPK-SIRT1-TFEB pathway. CNS Neurosci. Ther. 25: 796-807. Blocking peptide available for competition studies, sc-377299 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% stabilizer 3. Zi-Qi, L., et al. 2020. NADPH protects against kainic acid-induced excito- protein). toxicity via autophagy-lysosome pathway in rat striatum and primary cortical neurons. Toxicology 435: 152408. Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA 4. Ikeda, S., et al. 2021. YAP plays a crucial role in the development of car- STORAGE diomyopathy in lysosomal storage diseases. J. Clin. Invest. 131: e143173. Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. RESEARCH USE For research use only, not for use in diagnostic procedures.

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