The Effect of Lysine Supplementation of Rice Protein Upon Mineral Utilization in the Rat." (1978)
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Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 1978 The ffecE t of Lysine Supplementation of Rice Protein Upon Mineral Utilization in the Rat. Helen Kerry stevenson Tilton Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Tilton, Helen Kerry stevenson, "The Effect of Lysine Supplementation of Rice Protein Upon Mineral Utilization in the Rat." (1978). LSU Historical Dissertations and Theses. 3218. https://digitalcommons.lsu.edu/gradschool_disstheses/3218 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. 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Xerox University Microfilms 300 North Zaeb Road Ann Arbor, Michigan 48106 7815642 TXLTONi HELEN KCRRV STEVENSON THE EFFECT Of LYSINE SUPPLEMENTATION OF RlcE PROTEIN UPON MINERAL UTILIZATION IN THE *AT, THE LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COL,# PH.D.# 1*76 University. Mlcnjfilms International 300 n . z e e b r o a d , a n n a r b o r, mi 48106 THE EFFECT OF LYSINE SUPPL01ENTATICN OF RICE PROTEIN UPCN MINERAL OTILIZATICN IN THE RAT A Dissertation Submitted to the Graduate Faculty of the Louisiana State University and Agricultural and Mechanical College in partial fulfillment of the requirements for the degree of Doctor of Philosophy in The Department of Food Science by Helen Kerry Stevenson Tilton B. S. Louisiana State University, 1961 M. S. Louisiana State University, 1969 May, 1978 ACKNOWLEDGMENTS The author wishes to express special acknowledgment and gratitude to her major professor, Dr. Fred H. Hoskins, for his guidance and assistance during the course of this study. Sincere gratitude is expressed to Dr. Arthur F. Novak, Head of the Department of Food Science, for granting her an assistantship and laboratory facilities for her research and studies in this department. His counsel and continuing encouragement were a source of inspiration throughout these studies. Appreciation is extended to Dr. Robert Grodner, Dr. Joseph A. Liuzzo, Dr. Ramachandra Rao and Dr. Marie Brewer, other members of her advisory ccfimittee. The author is indebted to Dr. Glen Hembry, LSU Animal Science Department, for permitting use of the facilities of the ruminant nutrition laboratory and to his staff for technical assistance. The author is also indebted to Mrs. Margaret Cooksey for her expert assistance in the mineral analysis of the tissue samples and to the LSU Soils Laboratory for their facilities for atomic absorption spectrophotometry. The author expresses special gratitude to her husband, Joshua A. Tilton, whose encouragement, love, support and patience made this study possible and to her three children for their cooperation and understanding during the years of this graduate program. TABLE OF CONTENTS PAGE ACKNOWLEDGMENTS ................................... ii LIST OF TABLES ................................... iv LIST OF APPENDICES................................. vii ABSTRACT .......................................... viii USnTODtXTTICN...................................... 1 REVIEW OF LITEIRATURE................................ 4 Amino Acid Fortification of Cereal Grains ......... 4 Lysine - Potassium Interrelationships............ 9 Lysine and Calcium Absorption................... 9 Lysine and Iron Absorption....................... 10 MATERIALS AND MEEHODS................................ 12 RESULTS AND DISCUSSION.............................. 23 Weight Gain ................................... 23 Tissue Iron ................................... 26 Tissue C a l c i u m ............................... 36 Tissue P o t a s s i u m .............................. 44 Tissue Magnesium .............................. 53 Tissue Zinc ................................... 60 SUMMARY AND CONCLUSIONS ............................ 69 SELECTED BIBLIOGRAPHY .............................. 72 APPENDIX .......................................... 78 VITA ............................................. 96 iii LIST OF TART.ES TABLE PAGE 1. Experimental amino acid mixture.................. 16 2. Composition of diets ........................... 17 3. Jones and Foster salt mixture 1 2 ................ 18 4. Vitamin diet fortification mixture .............. 19 5. Mineral composition of diets .................... 20 6. Classification of rats by treatment.............. 21 7. Mean weight gain .............................. 25 8. Mean values of spleen iron of rats fed all dietary variations............................ 31 9. Mean values of muscle iron or rats fed all dietary variations ............................ 31 10. Mean values of liver iron of rats fed all dietary variations ............................ 32 11. Mean values of bone iron of rats fed all dietary variations ............................ 32 11A. Mean values of kidney iron of rats fed all dietary variations............................ 33 12. Comparison of tissue iron content of rats fed the rice reference diet with similar tissue of all other treatment groups................... 34 13. Comparison of tissue iron content of rats fed the casein reference diet with similar tissue of all other treatment groups................... 35 14. Mean values of liver calcium of rats fed all dietary variations........................ 39 iv PAGE Mean values of spleen calcium of rats fed all dietary variations..................... 39 Mean values of kidney calcium of rats fed all dietary variations ....................... 40 Mean values of muscle calcium of rats fed all dietary variations ....................... 40 Mean values of bone calcium of rats fed all dietary variations ....................... 41 Comparison of tissue calcium content of rats fed the rice reference diet with similar tissue of all other treatment .......... 42 Comparison of tissue calcium content of rats fed the casein reference diet with similar tissue of all other treatment groups ..................................... 43 Mean values of liver potassium of rats fed all dietary variations ....................... 48 Mean values of bone potassium of rats fed all dietary variations ....................... 48 Mean values of kidney potassium of rats fed all dietary variations ....................... 49 Mean values of spleen potassium of rats fed all dietary variations ....................... 49 Mean values of muscle potassium of rats fed all dietary variations ....................... 50 Comparison of tissue potassium content of rats fed the rice reference diet with similar tissue of all other treatment groups. 51 Comparison of tissue potassium content of rats fed the casein reference diet with similar tissue of all other treatment groups. 52 v PAGE Mean values of bone magnesium of rats fed all dietary variations ........................ 55 Mean values of kidney magnesium of rats fed all dietary variations ........................ 55 Mean values of spleen magnesium of rats fed all dietary variations ........................ 56 Mean values of muscle magnesium of rats fed all dietary variations ........................ 56 Mean values of liver magnesium of rats fed all dietary variations........................ 57 Comparison of tissue magnesium content of rats fed the rice reference diet with similar tissue of all other treatment groups ............ 58 Comparison of tissue magnesium content of rats fed the casein reference diet with similar tissue