SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DIRECTORY OF SERVICES
SRI NATHELLA SAMPATHU CHETTY CLINICAL LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) No.41, College Road, Chennai – 600 006 Ph: 2823 3556 / 2827 1616 / 2831 1913 Fax: 91-044-2825 4180 Email: [email protected]
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 1 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TABLE OF CONTENTS S.No CONTENTS Page No.
1. Title Page 1 - 1
2. Table of Contents 2 - 3
3. General Information of Laboratory Services 4 - 6
4. Contact Details 7 - 9
5. Quality Policy & Quality Objectives 10 – 10
6. Laboratory Charges 11 - 18
7. Package Tests 19 - 23
8. General Instruction on Sample Collection 24 – 27
9. DEPARTMENT OF HAEMATOLOGY & CLINICAL PATHOLOGY
28 – 33 Test Master List / Turn Around Time Acceptance and Rejection Criteria for 34 – 35 Collection Area, Hematology & Clinical Pathology 10. DEPARTMENT OF CLINICAL & SPECIAL BIOCHEMISTRY 36 – 42
Test Master List / Turn Around Time Acceptance and Rejection Criteria 43 – 45
11. DEPARTMENT OF MICROBIOLOGY & SEROLOGY
46 – 62 Test Master List / Turn Around Time Acceptance and Rejection Criteria 63 – 64 Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 2 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
12. DEPARTMENT OF HISTOPATHOLOGY & CYTOPATHOLOGY:
65 – 68 Test Master List / Turn Around Time Instructions for sending specimens to Histopathology 69 – 71
Acceptance and Rejection Criteria 72 – 72 13. DEPARTMENT OF CYTOGENETICS:
73 – 74 Test Master List / Turn Around Time
Acceptance and Rejection Criteria 75 – 76
14. Scope of Testing (NABL) 77 – 87
15. Validity of NABL Certificate 88 – 88
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 3 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Hospital Laboratory : Main Campus at Sankara Nethralaya
Sri Nathella Sampathu Chetty Clinical Laboratory (A Unit of Medical Research Foundation, Sankara Nethralaya)
The Legal name of the parent organization is “Medical Research Foundation”. The Organization is Registered under Societies Registration Act 1860 (S.No. 25 of 1978)
Address of SNSC Clinical Laboratory (A unit of Medical Research Foundation) SANKARA NETHRALAYA, No 41, (OLD No: 18), College Road Chennai – 600 006, Tamil Nadu, India Ph: 2823 3556 / 2827 1616 / 2831 1913 Fax: 91-044-2825 4180 Email: [email protected] / [email protected]
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 4 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Our other Primary Sample Collection Centres
1. Jagadguru Kanchi Sri Chandrasekarendra Saraswathi Nethra Nilayam, (SNSC Collection Centre – Pycrofts Road) No.21, Pycrofts Garden Road JKCN Centre Chennai – 600 006, Tamil Nadu, India. Phone: 28263556 / 28271616
2. Navasuja Sankara Nethralaya, (SNSC Collection Centre – NSN) No. 73, Venkata Krishna Road, Raja Annamalaipuram, Chennai – 600 028, Tamil Nadu, India. Phone: 24611273 / 24610118
3. C.U.Shah Sankara Nethralaya (SNSC Collection Centre-REH) No. 8, GST Road, St.Thomas Mount, Gunidy Chennai 600 016, Tamil Nadu, India. Ph No: 91-044-22346022, 22344474
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 5 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
The Laboratories under SNSC Clinical Lab includes: 1. Clinical Biochemistry Main laboratory , Main Campus 2. Clinical Pathology Main laboratory, Main Campus 3. Clinical Hematology Main laboratory, Main Campus 4. Histopathology and Cytopathology 3rd floor, KNBIRVO block, Main campus 5. Genetics and Molecular biology 4th floor, KNBIRVO block, Main Campus 6. Special Clinical Biochemistry 5th floor, KNBIRVO block, Main Campus 7. Microbiology and Serology 6th floor, KNBIRVO block, Main campus 8. SNSC Collection Centre -JKCN , 21, Pycrofts Garden Road 9. SNSC Collection Centre -NSN, 73, Venkata Krishna Road, Raja Annamalaipuram 10. SNSC Collection Centre -REH, 8, GST Road, St.Thomas Mount
The primary samples are collected at : • Main laboratory at SN main • SNSC Collection Centre – Pycrofts Road, (Only as collection center) • SNSC Collection Centre - NSN , (Only as collection center) • SNSC Collection Centre – REH centers, (Only as collection center)
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 6 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Heads of the Laboratories S.No. Name Designation, Authorized Signatory, Contact details 1. Dr.S.B.Vasanthi Management Representative (MR) NABL, Director, SNSC Laboratory Contact Details: 28271616, Ext:1101 Email: [email protected] 2. Dr.H.N.Madhavan Deputy Management Representative (MR) NABL, Director, Microbiology & Serology NABL Authorized Signatory Contact Details: 28271616, Ext:2550 Email: [email protected] 3. Dr.N.Angayarkanni HOD – Clinical & Special Biochemistry NABL Authorized Signatory Contact Details: 28271616, Ext:1304/ 1341 Email: [email protected] 4. Dr.Doreen Gracias Deputy Quality Manager–NABL HOD– Cl.Hematology & Cl.Pathology NABL Authorized Signatory Contact Details: 28271616, Ext:1304/ 1341 Email: [email protected]
5. Dr.J.Biswas Director – Histopathology
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 7 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
NABL Authorized Signatory Contact Details: 28271616, Ext:1302 Email: [email protected] 6. Dr.S.Krishna Kumar HOD – Histopathology NABL Authorized Signatory Contact Details: 28271616, Ext:1302 Email: [email protected] 7. Dr.K.Lily Therese HOD –Microbiology & Serology NABL Authorized Signatory Contact Details: 28271616, Ext:1301/1305 Email: [email protected] 8. Dr.R.Harini Assistant Professor - Clinical Biochemistry NABL Authorized Signatory Contact Details: 28271616, Ext:1103 Email: [email protected] 9. Dr.S.Mathavan HOD – Cytogenetics Contact Details: 28271616, Ext:1308/1898 Email: [email protected]
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 8 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Quality Manager S.No. Name Department 1. Dr.N.Angayarkanni Head, Clinical & Special Biochemistry
Technical Manager S.No. Name Department 1. Ms.R.Punitham Clinical & Special Biochemistry
Deputy Technical Managers S.No. Name Department 1. Ms.Saumya.T.S. Cl.Hematology & Cl.Pathology 2. Ms.Gayathri Microbiology & Serology 3. Ms.K.Vanitha Histopathology
LABORATORY TIMINGS
Monday to Saturday: 7.30 am – 5.00 pm (Sunday: Holiday) REH and NSN: 10.00am to 12.30pm
After 6.00 pm samples will be collected by ward sister and samples will be sent to either Apollo Hospitals / Lister Laboratory (Consultants / Anesthetist will decide depending upon the test details)
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 9 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Quality Policy “We at SNSC Clinical laboratory commit to provide Quality Medical Laboratory service in terms of generating reliable patient test reports, on time, using appropriate technology of international standards through committed and competent staff, who ensure to abide by the policies and procedures of the laboratory at all times with complete awareness of the required documentations.”
Objectives 1. To comply with ISO15189:2012 standards at all time. 2. To maintain excellence in medical laboratory services using standard technology. 3. To ensure compliance with the statutory and regulatory requirements. 4. To ensure adequate resources and staff competence, enabling effective professional services. 5. To complete the laboratory tests and report the results in an effective and timely manner. 6. To ensure continued patient satisfaction.
Date: 10.09.14 Dr. S.B. Vasanthi Director-Laboratory Services Management Representative Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 10 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
LAB TARIFF DETAILSs with effect from 01.08.18
TEST TEST DETAILS Amount CODE NO (Rs) 001 HAEMOGLOBIN (HB) AND HAEMATOCRIT 160 002 TOTAL RBC COUNT ) 90 003 BLOOD INDICES 130 004 TOTAL W B C COUNT AND DIFF.COUNT 160 005 E.S.R. (WESTERGREN S) 140 006 PLATELET COUNT 140 007 RETICULOCYTE COUNT 140 008 ABSOLUTE EOSINOPHIL COUNT 130 009 BLOOD SMEAR STUDY 220 010 BLOOD SMEAR PARASITES 270 012 SICKLE CELL PREPARATION 140 014 HAEMOGLOBIN ELECTROPHORESIS 960 015 BLOOD GROUP AND RH TYPING 240 018 URINE ROUTINE ANALYSIS 140 019 URINE BENCE-JONES PROTEIN 140 020 MOTION ROUTINE ANALYSIS 140 021 OCCULT BLOOD: URINE 90 025 PROTHROMBIN TIME (PT) 290 026 PARTIAL THROMBOPLASTIN TIME (PTT) 290 027 BLOOD COLLECTION FOR CROSS MATCHING 1160 028 URINE - SUGAR, ALBUMIN, ACETONE 40 029 URINE – LFT 40 030 MOTION EXAM FOR OCCULT BLOOD 130 101 BLOOD GLUCOSE (REF.CHK) 120 102 PLASMA GLUCOSE (F/R/PP) 120 103 GLUCOSE TOLERENCE TEST (GTT) 490 104 GESTATIONAL DIABETES MELLITUS (GDM) 490 105 UREA AND CREATININE 270 106 CHOLESTEROL 180
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 11 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
108 ELECTROLYTES (NA K) 400 109 ELECTROLYTES (NA, K,CL,HCO) 490 110 CALCIUM AND INORG PHOSPHROUS 290 112 CERULOPLASMIN 900 113 LIVER FUNCTION TESTS 1280 114 BILIRUBIN (TOTAL AND DIRECRT) 280 115 TOTAL PROTEINS AND A. G. RATIO 220 116 ALKALINE PHOSPHATASE 220 117 ALT (SGPT) 220 118 AST (SGOT) 240 121 ELECTROPHORESIS FOR SERUM/URIN 600 122 ELECTROPHORESIS FOR LIPOPROTEI 630 123 LIPID PROFILE 910 124 TRIACYL GLYCEROL (TRIGLYCERIDE 240 125 PYRUVATE/LACTATE (SPECIFY) 1160 126 ANGIOTENSIN CONVERTINGENZYME 1130 127 INBORN ERRORS OF METABOLISM 400 128 CSF ANALYSIS 670 129 GLUCOSE 6 PHOSPHATE DEHYDROGEN 600 130 LACTATE DEHYDROGENASE (LDH) 1320 131 VITAMIN A (HPLC) 1320 132 HOMOCYSTINURIA SCREENING 140 133 PAPERCHROMATOGRAPHY FOR GAL/SG 580 134 PAPR CHROMATOGRAPHY FOR ORT/HM 760 135 AMINO ACIDS PROFILE, SERUM 1880 136 AMINO ACIDS PROFILE, URINE 1880 137 SERUM AMYLASE 400 138 HOMOCYSTINE - PLASMA 1880 141 VITAMIN E (HPLC) 1320 142 VITAMIN C 960 143 GLUTATHIONE 560 144 GLUTATHIONE PEROXIDASE 920 145 SUPEROXIDE DISMUTASE 560 146 THIO BARBITURIC ACID REACTIVE SUBSTANCES 230 151 HBA1C 500 152 MICRO ALBUMIN 640
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 12 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TRACE 153 ELEMENTS:(COPPER/ZINC/SELENIUM/IRON)(SPECIFY) 420 154 GAMMA GT (GGTP) 150 202 NON TREPONEMAL (RPR)AND TREPONEMAL (TPHA) 600 203 RA FACTOR (NEPHOLOMETRY / TURBIDOMETRY) 220 204 BRUCELLA AGGLUTINATION 220 205 WIDAL TEST 220 206 FLUORESCENT ANTI-NUCLEAR ANTIBODY (FANA) 1520 207 ABS. TO. TOXOPLASMA ANTIGEN 1520 210 ABS. TO RUBELLA VIRUS 1520 211 ABS. TO CYTOMEGALOVIRUS 1520 212 ABS. TO HERPES SIMPLEX VIRUS 1520 213 IGG AND IGM ABS. TO EBV (VCA) 1520 217 ASO (LATEX) 290 218 CRP (NEPHOLOMETRY / TURBIDOMETRY) 270 219 LYSOZYME ASSAY 400 220 ABS. TO HIV 1 AND HIV 2 1240 221 WESTERN BLOT FOR ABS. TO HIV 1 & HIV 2 4650 222 HBSAG 670 223 ABS. TO HCV 490 233 IGG ABS. TO AQUAPORIN 4 (NMO) IF / ELISA 4950 233A IGG ABS. TO AQUAPORIN 4 (NMO) IF / MOG 6300 234 ANTIBODIES TO CANCA (PR3) 1580 235 ANTIBODIES TO PANCA (MPO) 1580 260 ABS. TO HEPATITIS BS AG 760 263 QUANTIFERON-TB TEST 3480 301 ROUTINE WORK-UP BELOW-60 720 302 ROUTINE WORK-UP ABOVE 60 990 303 ROUTINE WORK-UP DIABETIC 990 304 UVEITIS WORK UP BELOW 60 5120 305 UVEITIS WORK-UP ABOVE 60 5390 306 UVEITIS WORK-UP DIABETIC 5390 307 EALES WORK-UP BELOW 60 6840 308 EALES WORK-UP ABOVE 60 7110 309 EALES WORK-UP DIABETIC 7110 310 COMPLETE HAEMOGRAM 1170 Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 13 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
311 ANAEMIA WORK UP 2690 BASIC COAGULATION TESTS (PLATELT,BT,CT,CLOT 312 RET,PT AND PTT) 1010 313 COAGULATION PROFILE 2900 314 SARCOIDOSIS WORK UP 2360 315 EALES - SPECIAL BIOCHEMICAL TESTS 5870 316 TORCHES SCREENING 6690 317 HBS AG, HCV,RPR AND TPHA 1760 PCR FOR PAN FUNGAL, EUBACTERIAL AND P.ACNE 318 GENOME 7250 319 DCR WORK-UP (BELOW 60) 3730 320 DCR WORK-UP (ABOVE 60) 4000 321 DCR WORK-UP (DIABETIC) 4000 322 HUMAN AMNIOTIC MEMBRANE (HAM) 1520 375 PCR FOR MYCOBACTERIUM TUBERCULOSIS AND HSV 4830 378 PP65 ANTIGEN ASSAY AND PCR FOR CMV 5010 381 ROUTINE WORK UP FT 670 382 CHEMO PACKAGE 2580 401 GRAM S STAIN 140 402 KOH / CALCOFLOUR STAIN 140 403 GIEMSA STAIN 140 404 AFB STAIN 140 407 IMM.FLUOR.STAIN.HSV 600 411 BACTERIAL CULTURE 760 412 FUNGAL CULTURE 490 413 CHLAMYDIA TRACHOMATIS CULTURE 760 414 ACANTHAMOEBA CULTURE 290 415 MYCOBACTERIAL CULTURE (BACTEC METHOD) 860 416 HSV ISOLATION 760 419 ADENO/RUBELLA ISOLATION 760 420 PCR FOR MYCOBACTERIUM TUBERCULOSIS (MTB) 2420 421 PCR FOR CYTOMEGALOVIRUS (CMV) 2420 422 PCR FOR HERPES SIMPLEX VIRUS (HSV) 2420 423 PCR FOR VARICELLA ZOSTER VIRUS (VSV) 2420 424 PCR FOR ADENOVIRUS 2420 425 PCR FOR CHLAMYDIA TRACHOMATIS 2420
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 14 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
426 PCR FOR EUBACTERIAL GENOME 2420 427 PCR FOR PAN FUNGAL GENOME 2420 428 PCR FOR PROPIONIBACTERIUM ACNE 2420 429 PCR FOR TOXOPLASMA GONDII 2420 430 PP65 ANTIGEN CMV 2590 431 RUBELLA ISOLATION 690 434 NIGROSIN STAIN 130 451 PCR FOR CHLAYMYDIA PNEUMONIAE 2250 452 RT - PCR FOR RUBELLA 3160 453 PCR FOR HLA B 27 3720 ANTIBIOTIC (FIRST LINE DRUGS) SENSITIVITY - 456 (BACTEC METHOD) 4330 458 BLOOD CULTURE (BACTEC METHOD) 1130 459 HIV - 1 REAL TIME PCR 4650 460 REAL TIME PCR FOR CHIKUNGUNYA VIRUS 5550 461 REAL TIME PCR FOR CYTOMEGLO VIRUS (CMV) 4650 462 REAL TIME PCR FOR HERPES SIMPLEX VIRUS (HSV) 4650 REAL TIME PCR FOR MYCOBACTERIUM 463 TUBERCULOSIS (M. T 5550 464 REAL TIME PCR FOR HEPATITIS B VIRUS 5160 465 REAL TIME PCR FOR HEPATITIS C VIRUS 5160 467 PCR BASED DNA SEQUENCING 3980 471 PCR FOR MYCOBACTERIUM FORTUITUM 2050 472 PCR FOR MYCOBACTORIUM CHELONAE 2050 473 PCR FOR LEPTOSPIRA 2420 474 PCR FOR SALMONELLA TYPHI 2420 486 PCR FORPNEUMOCYSTIS JIROVECII 2250 487 REVERSE TRANSCRIPTASE PCR FOR M. TUBERCULOSIS 3100 489 REAL TIME PCR FOR DENGUE FEVER SEROTYPES 1-4 3720 490 INVITRO ANTIFUNGAL SENSITIVITY TEST 1000 491 PCR FOR MYCOBACTERIUM SP.(HSP65 GENE) 2420 492 DNA SEQUENCING OF PCR AMPLIFIED PRODUCTS 1560 GENE XPERT MTB/RIFAMPICIN RESISTANCE 493 DETECTION ASSAY 1740 496 REAL TIME PCR FOR VARICELLA ZOSTER VIRUS 4650 497 PRODUCT FOR DNA SEQUENCING LOADING 210
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 15 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
499 PCR FOR PYTHIUM INSIDIOSUM 2420 501 EYE BALL 1580 501B EYE BALL (F AND P) 1590 502 EXENTERATED SPECIMEN 1580 502B EXENTERATED SPECIMEN (F AND P) 1590 503 EVISCERATED SPECIMEN 840 503B EVISCERATED SPECIMEN (F AND P) 1590 504 CORNEAL BUTTON 840 504B CORNEAL BUTTON (F AND P) 1590 505 EPIRETINAL MEMBRANE 630 505B EPIRETINAL MEMBRANE (F AND P) 1590 506 SUBRETINAL MEMBRANE 630 506B SUBRETINAL MEMBRANE (F AND P) 1590 507 BIOPSY - LID 630 507B BIOPSY - LID (F AND P) 1590 508 BIOPSY - CONJUNCTIVA 630 508B BIOPSY - CONJUNCTIVA (F AND P) 1590 509 BIOPSY - CORNEA 840 509B BIOPSY - CORNEA (F AND P) 1590 510 BIOPSY - ORBIT 1580 510B BIOPSY - ORBIT (F AND P) 1590 511 IRIS TISSUE 630 511B IRIS TISSUE (F AND P) 1590 512 AQUEOUS ASPIRATE 320 513 VITEROUS ASPIRATE 320 514 SUBRETINAL FLUID 320 515 SMEAR - CYTOLOGY - LID 320 516 SMEAR - CYTOLOGY - CONJUNCTIVA 320 517 SMEAR - CYTOLOGY - CORNEA 320 518 FNAB 320 519 FNAC 320 520 IMMUNOHISTOCHEMICAL STAIN 1580 521 IOL CYTOLOGY 320 522 SPECIAL STAINS - SMEAR 320 523 SPECIAL STAINS - SECTIONS 530 524 AMYLOID STAIN 530
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 16 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
525 MUCIN STAIN 530 526 COLLAGEN STAIN 530 527 ELASTIC STAIN 530 528 GMS STAIN 530 529 GRAM STAIN 530 530 CALCIUM STAIN 530 531 AFB-STAIN 530 532 OIL O RED STAIN 530 533 PERLS IRON STAIN 530 534 GIEMSA-STAIN 530 535 FONTANNA MASSON STAIN 530 536 TOLUDINE BLUE STAIN 530 537 SMALL SIZED SPECIMENS (3MM-5MM) 630 FROZEN AND PERMANENT SECTION-SMALL SIZED 537B SPECIMEN 1590 538 MEDIUM SIZED SPECIMENS (10MM) 840 FROZEN AND PERMANENT SECTION-MEDIUM SIZED 538B SPECIMEN 1480 539 LARGE SIZED SPECIMENS (GREATER THAN 20MM) 1580 FROZEN AND PERMANENT SECTION-LARGE SIZED 539B SPECIMEN 1480 550 OTHERS (SPECIFY) 280 574 SLIDE/PARAFFIN BLOCK GIVEN TO PATIENT 160 575 BONE MARROW ASPIRATE 1050 601 MANTOUX (RA/LA) 180 701 CHROMOSOMAL STUDY (PBLC) 2730 712 DNA EXTRACTION (MINI KIT) 560 713 DNA EXTRACTION (MAXI KIT) 1670 CYP1B1 GENE SCREENING FOR CONGENITAL 714 GLAUCOMA 7120 MYOC GENE SCREENING FOR OPEN ANGLE 715 GLAUCOMA 7130 SCREENING THE THREE PRIMARY MITOCHONDRIAL MUTATIONS FOR LEBER~S HEREDITARY OPTIC 723 NEUROPATH 5120 725 PAX6 GENE SCREENING FOR ANIRIDIA 8390 726 RS1 GENE SCREENING FOR RETINOSCHISIS 7270 Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 17 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
733 NDP GENE SCREENING FOR NORRIE DISEASE 7570 734 EYE PANEL TESTING USING NGS 25470 735 RARE DISEASE PANEL TESTING USING NGS 37040 736 MST (Mutation Specific Test) 5750 737 MLPA (Multiplex Ligation dependent Probe Amplification) 4600 738 LHON Gene Screening 24000 739 CGM Gene Screening 12000 ORNITHINE AMINO TRANSFERASE (OAT) GENE 740 SCREENING 13400 OUT SOURCED TESTS 801 THYROID PROFILE(FT3,FT4,TSH) 680 808 ANTI PHOSPHOLIPID ANTIBODY 1680 809 CD4 COUNTS 1120 811 PROTEIN C (ACTIVITY) 3970 812 PROTEIN S (ACTIVITY) 3970 813 VITAMIN B12 1100 816 FOLATE RBC 1920 820 URIC ACID 220 830 SERUM ACID PHOSPHATASE 250 845 SERUM IRON, TIBC AND PERCENTAGE SATURATION 780 846 CORTISOL 600
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 18 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
PACKAGE TESTS
ROUTINE WORK UP
S. No. Test Test Code: 301 (BELOW 60) Test Code: 302 (ABOVE 60) Test Code:303 (DIABETIC) code Charges: Rs.720/- Charges: Rs.990 Charges: Rs.990
1 001 Haemoglobin (Hb) and Haemoglobin (Hb) and Haemoglobin (Hb) and Haematocrit (PCV) Haematocrit (PCV) Haematocrit (PCV) 2 004 Total W.B.C. Count and Total W.B.C. Count and Total W.B.C. Count and Differential Count Differential Count Differential Count 3 005 E.S.R. E.S.R. E.S.R. 4 102 Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) 5 018 Urine Routine Analysis Urine Routine Analysis Urine Routine Analysis 6 105 Plasma Urea and Creatinine Plasma Urea and Creatinine
UVEITIS WORKUP
S. No. Test Test Code: 304 (BELOW 60) Test Code: 304 (ABOVE 60) Test Code:306 (DIABETIC) code Charges: Rs.5120 Charges: Rs. Rs.5390 Charges: Rs.5390
1 001 Haemoglobin (Hb) and Haemoglobin (Hb) and Haemoglobin (Hb) and Haematocrit (PCV) Haematocrit (PCV) Haematocrit (PCV) 2 004 Total W.B.C. Count and Total W.B.C. Count and Total W.B.C. Count and Differential Count Differential Count Differential Count 3 005 E.S.R. E.S.R. E.S.R. 4 102 Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) 5 018 Urine Routine Analysis Urine Routine Analysis Urine Routine Analysis 6 105 Plasma Urea and Creatinine Plasma Urea and Creatinine 7 601 Mantoux (RA/ LA) Mantoux (RA/ LA) Mantoux (RA/ LA) 8 020 Motion Routine Analysis Motion Routine Analysis Motion Routine Analysis 9 202 Non Treponemal (RPR) and Non Treponemal (RPR) and Non Treponemal (RPR) and Treponemal (TPHA) Treponemal (TPHA) Treponemal (TPHA) 10 203 Rheumatoid Arthritis (RA) factor Rheumatoid Arthritis (RA) Rheumatoid Arthritis (RA) (latex) factor (latex) factor (latex) 11 204 Brucella Agglutination Test Brucella Agglutination Test Brucella Agglutination Test 12 206 Anti Nuclear Antibody Anti Nuclear Antibody Anti Nuclear Antibody (Fluorescent & ELISA) (Fluorescent & ELISA) (Fluorescent & ELISA) 13 207 Abs. to Toxoplasma gondii (IgG & Abs. to Toxoplasma gondii Abs.toToxoplasma gondii (IgG IgM) (IgG & IgM) & IgM)
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 19 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
EALES WORKUP
S. Test Test Code: 307 (BELOW 60) Test Code: 308 (ABOVE 60) Test Code:309 No. code Charges: Rs.6840 Charges: Rs.7110 (DIABETIC) Charges: Rs.7110 1 001 Haemoglobin (Hb) and Haemoglobin (Hb) and Haemoglobin (Hb) and Haematocrit (PCV) Haematocrit (PCV) Haematocrit (PCV) 2 004 Total W.B.C. Count and Total W.B.C. Count and Total W.B.C. Count and Differential Count Differential Count Differential Count 3 005 E.S.R. E.S.R. E.S.R. 4 006 Platelet count Platelet count Platelet count 5 007 Reticulocyte count Reticulocyte count Reticulocyte count 6 009 Blood smear study Blood smear study Blood smear study 7 102 Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) 8 018 Urine Routine Analysis Urine Routine Analysis Urine Routine Analysis 9 105 Plasma Urea and Creatinine Plasma Urea and Creatinine 10 601 Mantoux (RA/LA) Mantoux (RA/LA) Mantoux (RA/LA) 11 312 Basic Coagulation Tests: Basic Coagulation Tests: Basic Coagulation Tests: Bleeding Time Bleeding Time Bleeding Time Clotting Time Clotting Time Clotting Time Clot Retraction Clot Retraction Clot Retraction 025 Prothrombin Time (PT) Prothrombin Time (PT) Prothrombin Time (PT) 026 Activated Partial Thromboplastin Time Activated Partial Thromboplastin Activated Partial (APTT) Time Thromboplastin Time (APTT) (APTT) 12 012 Sickle Cell Preparation Sickle Cell Preparation Sickle Cell Preparation 13 014 Hemoglobin Electrophoresis Hemoglobin Electrophoresis Hemoglobin Electrophoresis 14 202 Non Treponemal (RPR) and Non Treponemal (RPR) and Non Treponemal (RPR) and Treponemal (TPHA) Treponemal (TPHA) Treponemal (TPHA) 15 203 Rheumatoid Arthritis (RA) factor Rheumatoid Arthritis (RA) factor Rheumatoid Arthritis (RA) (Latex) (Latex) factor (Latex) 16 206 Anti Nuclear Antibody Anti Nuclear Antibody ( Fluorescent & ELISA) ( Fluorescent & ELISA) ( Fluorescent & ELISA) ( Fluorescent & ELISA) 17 126 Serum Angiotensin converting Enzyme Serum Angiotensin converting Serum Angiotensin (ACE) Enzyme converting Enzyme (ACE) (ACE)
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 20 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
S. No. Test code Test Code: 310 Test Code: 311 COMPLETE HEMOGRAM ANEMIA WORK UP Charges: Rs.1170 Charges: Rs.2690 1 001 Haemoglobin (Hb) and Haemoglobin (Hb) and Haematocrit (PCV) Haematocrit (PCV) 2 004 Total W.B.C. Count and Total W.B.C. Count and Differential Count Differential Count 3 005 E.S.R. E.S.R. 4 002 Total RBC Count Total RBC Count 5 006 Platelet count Platelet count 6 003 Blood Indices Blood Indices 7 009 Blood Smear Study Blood Smear Study 8 007 Reticulocyte Count Reticulocyte Count 9 012 Sickle Cell Preparation 10 014 Hemoglobin Electrophoresis 11 020 Motion Routine Analysis 12 105 Plasma Urea and Creatinine 13 845 Serum Iron and TIBC & %Saturation
S. No. Test code Test Code: 312 Test Code: 313 BASIC COAGULATION TESTS COAGULATION PROFILE Charges: Rs.1010 Charges: Rs.2900 1 Bleeding Time Bleeding Time 2 Clotting Time Clotting Time 3 Clot Retraction Clot Retraction 4 025 Prothrombin Time (PT) Prothrombin Time (PT) 5 026 Activated Partial Thromboplastin Time Activated Partial Thromboplastin Time (APTT) (APTT) 6 006 Platelet count Platelet count 7 Euglobulin Lysis Time 8 Fibrinogen Assay 9 Factor XIII 10 015 Blood Group and Rh Typing
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 21 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
S. No. Test code Test Code: 314 SARCOIDOSIS WORKUP Charges: Rs.2360 1 005 ESR 2 110 Serum Calcium and Inorg.phosphorus 3 115 Serum Total Proteins & A.G Ratio 4 126 Serum Angiotensin Converting Enzyme 5 219 Serum Lysozyme Assay 6 601 Mantoux (RA/LA)
S. No. Test code Test Code: 315 SPECIAL BIOCHEMICAL TESTS Charges: Rs.5870 1 146 Blood Thiobarbituric Acid Reactive Substances 2 141 Serum Vitamin E (HPLC) 3 142 Plasma Vitamin C 4 131 Serum Vitamin A (HPLC) 5 145 Blood Superoxide dismutase 6 144 Blood Glutathione Peroxidase 7 143 Blood Glutathione S. No. Test code Test Code: 316 TORCHES SCREENING Charges: Rs.6690 1 207 Abs. to Toxoplasma gondii (IgG & IgM) 2 210 Abs. to Rubella Virus (IgG & IgM) 3 211 Abs. to CMV (IgG & IgM) 4 212 Abs. To HSV (IgG & IgM) 5 202 Non Treponemal (RPR) and Treponemal (TPHA)
S. No. Test code Test Code: 381 ROUTINE WORK UP (FAST TRACK) Charges: Rs.670 1 001 Haemoglobin (Hb) and Haematocrit (PCV) 2 002 Total RBC Count 3 004 Total W.B.C. Count and Differential Count 4 006 Platelet Count 5 102 Plasma Glucose (F/R/PP)
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DCR WORKUP S. Test Test Code: 319 (BELOW 60) Test Code: 320 (ABOVE 60) Test Code: 321 (DIABETIC) No. code Charges: Rs.3730 Charges: Rs.4000 Charges: Rs.4000 1 001 Haemoglobin (Hb) and Haemoglobin (Hb) and Haemoglobin (Hb) and Haematocrit (PCV) Haematocrit (PCV) Haematocrit (PCV) 2 004 Total W.B.C. Count and Total W.B.C. Count and Total W.B.C. Count and Differential Count Differential Count Differential Count 3 005 E.S.R. E.S.R. E.S.R. 4 102 Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) Plasma Glucose (F/ R/ PP) 5 018 Urine Routine Analysis Urine Routine Analysis Urine Routine Analysis 6 105 Plasma Urea and Creatinine Plasma Urea and Creatinine 7 312 Basic Coagulation Tests: Basic Coagulation Tests: Basic Coagulation Tests: Bleeding Time Bleeding Time Bleeding Time Clotting Time Clotting Time Clotting Time Clot Retraction Clot Retraction Clot Retraction 025 Prothrombin Time (PT) Prothrombin Time (PT) Prothrombin Time (PT) 026 Activated Partial Thromboplastin Time Activated Partial ActivatedPartial (APTT) Thromboplastin (APTT) Thromboplastin Time (APTT) 006 Platelet count Platelet count Platelet count 8 015 Blood Group and Rh Typing Blood Group and Rh Typing Blood Group and Rh Typing 9 317 HbsAg, Abs to HCV, RPR and TPHA HbsAg, Abs to HCV, RPR and HbsAg, Abs to HCV, RPR TPHA and TPHA
S. No. Test code Test Code: 382 CHEMO PACKAGE Charges: Rs.2580 1 001 Haemoglobin (Hb) and Haematocrit (PCV) 2 004 Total W.B.C. Count and Differential Count
3 005 E.S.R. 4 006 Platelet count 5 018 Urine Routine Analysis 6 102 Plasma Glucose (F/ R/ PP) 7 105 Plasma Urea and Creatinine 8 109 Electrolytes (NA, K,CL,HCO) 9 114 Bilirubin (Total and Direct) 10 116 Alkaline Phosphatase 11 117 ALT (SGPT) 12 118 AST (SGOT) Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 23 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
GENERAL INSTRUCTION ON SAMPLE COLLECTION
A.CLINICAL BIOCHEMISTRY: Instructions for routine blood investigation: 1. Fasting plasma Glucose (FBS) For a fasting blood glucose test, do not eat or drink anything other than water for at least 8 to 12 hours before the blood sample is taken. Tea, coffee, alcohol intake and smoking are not permitted during this period. Reasonable amount of water intake is permitted. If diabetes, your doctor may ask you to wait until you have had your blood collected before taking your morning dose of insulin or diabetes medicine. 2. 2-hour Postprandial (post lunch) plasma glucose: For a 2 hour postprandial test, you need to have your blood collected exactly 2 hours after a regular lunch. He /She can have water and his/her usual medicines. PP blood sugar to be collected exactly after 2 hours of food (From the food intake time). The instructions for the same will be given by the Consultant /Physicians /Physician Secretary /SFC (Surgery Fixing Center). It will be ensured by the Laboratory Enquiry Secretary and counter checked by Technician/ Lab Assistant during interaction with the patients at the time of collection. 3. Random plasma glucose (RBS): No special preparation is required before having a random blood sugar test. The patient is required to be in non fasting state, hence blood samples will be collected within 2 hours of last meal intake irrespective of time of the day. 4. Lipid profile Patient should fast for 12 hours before blood collection. Fasting should be no food or drink except for water
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Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
B. SPECIAL BIOCHEMICAL CLINICAL INVESTIGATION: 1. Plasma Amino acid Profile (HPLC) 2. Plasma Homocysteine 3. Paper Chromatography for Plasma Ornithine/ any amino acid 4. Paper Chromatography for Plasma Galactose / other sugars. 5. Microalbuminuria Fasting Specimen: For all the above mentioned test the patients are instructed to have his/her dinner the previous night not later than 9 pm and following that not to eat or drink except water till he/ she reports to the laboratory (fasting period should be between 8 to 12 hours).
1.Instructions to the patients underwent FFA (Fundus Fluorescein Angiography) In the above mentioned conditions the patients are instructed not to give blood for 24 hours after the FFA for the following test, 1. Angiotensin Converting Enzyme (ACE)
2. Instructions to Patients coming for serum Vitamin A testing: Should not take vitamin A tablet, carrot not to be taken in the previous 24 hours. Blood should be collected in fasting condition.
3. Instructions to patients for Serum Iron, TIBC and Percentage saturation testing: Patient coming for the above test should report for blood collection at the main lab between 9 to 11 am. Should not take any iron medication for 3 days. The test is out sourced.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
4. Instructions to patients coming for Gestational Diabetes Mellitus (GDM) Patient should fast for 12 hours before blood collection. Fasting should be no food or drink except for water 82.5g monohydrate glucose in 250ml of water or 100g anhydrous glucose load will be given post fasting sample collection. • First blood should be collected in fasting condition. • 2nd sample 1 hour after glucose • 3rd sample 2hrs after glucose taken. • 4th samples 3 hrs after glucose.( If 100g load given)
5. Instructions to patients coming for Oral Glucose Tolerance test (OGTT) Patient should fast for 12 hours before blood collection. Fasting should be no food or drink except for water 82.5g monohydrate glucose in 250ml of water glucose load will be given post fasting sample collection. o First blood should be collected in fasting condition. o 2nd sample 1 hour after glucose o 3rd sample 2hrs after glucose taken.
6. IEM (Inborn Errors of metabolism): Three containers will be provided for the patient, one of the three containers labeled ‘F’ (fasting) and the other two containers labeled ‘R’ (Random). Patient should collect the fasting urine in the ‘F’ container and the random urine in the ‘R’ containers. Provide 3 urine containers with pinch of sodium azide.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
C. HAEMATOLOGY: Random blood collection is done for routine hematological test.
D.CYTOGENETICS: (Instructions to chromosomal study)
1. Random blood collection is done for above mentioned test. 2. The patient should not be under any chemotherapy, immunosuppressive drugs or affected by immunosuppressive disease and septicemia, which affect mitotic index and sterility of the culture.
E.MICROBIOLOGY:
The patients coming for Microbiological test should not take any antibiotic therapy prior to investigation.
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 27 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DEPARTMENT OF
HAEMATOLOGY &
CLINICAL PATHOLOGY
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 28 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF HAEMATOLOGY
S Code Name of the Turn Specimen Anticoagulant Storage/ NO NO test around required (vacutainer tubes) Temperature time (TAT) 1 001 *Hb and PCV 1 hour 2ml blood 3.6mg K2 EDTA Upto 24 1ml (<10 yrs) (Lavender) hours/ 2 80C
2 002 *Total RBC 1 hour 2ml blood 3.6mg K2 EDTA Upto 24 count 1ml (<10 yrs) (Lavender) hours/ 2 80C
3 003 *Blood Indices 1 hours 2ml blood 3.6mg K2 EDTA Upto 24 1ml (<10 yrs) (Lavender) hours/ 2 80C
4 004 *TC and DC 1 hour 2ml blood, 3.6mg K2 EDTA Upto 24 1ml (<10 yrs) 2 (Lavender) hours/ 2 80C Smears 5 005 *ESR 1 hour 1ml blood 3.8% Sodium Citrate Upto 24 (Black) hours/ 2 80C 6 006 *Platelet count 1 hour 2ml blood 3.6mg K2 EDTA Upto 24 1ml (<10 yrs) (Lavender) hours/ 2 80C 7 007 *Reticulocyte 2 1/2 hour 2ml blood 3.6mg K2 EDTA Upto 24 count 1ml (<10 yrs) (Lavender) hours/ 2 80C 8 008 *Absolute 2 1/2 hour 2ml blood 3.6mg K2 EDTA Upto 24 eosinophil count 1ml (<10 yrs) (Lavender) hours/ 2 80C
9 009 *Blood smear 2 1/2hours 2ml blood 3.6mg K2 EDTA Upto 24 study 1ml (<10 yrs) 2 (Lavender) hours/ 2 80C Smears 10 010 *Blood smear 2 1/2 hours 2ml blood in 3.2% citrated blood Upto 24 0 parasite EDTA and 2.7ml (Blue), 3.6mg K2 hours/ 2 8 C blood EDTA (Lavender) of 3.2% citrated blood, 1 wet mount, 2 thin and thick smears (Ref SNSC/CM/3.1) 11 012 *Sickle cell 24 hours 2ml blood 3.6mg K2 EDTA Upto 24 preparation 1ml (<10 yrs) (Lavender) hours/ 2 80C 13 014 Hemoglobin 48 hours 2ml blood 3.6mg K2 EDTA 1 week electrophoresis (Lavender) / 2 80C 14 015 *Blood group 2 hours 2ml blood 3.6mg K2 EDTA Upto 24
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
and Rh typing 1ml (<10 yrs) (Lavender) hours/ 2 80C 15 025 *Prothrombin 1 hour 2.7ml blood 3.2% citrated blood Upto 24 time (PT) 1ml (<10 yrs) (Light Blue) hours/ 2 80C 16 026 *Partial 1 hour 2.7ml blood 3.2% citrated blood Upto 4 hours/ thromboplastin 1ml (<10 yrs) (Light Blue) 2 80C time(PTT) 17 312 Bleeding time 30 minutes 18 312 Clotting time 30 minutes 3 ml blood 19 312 Clot retraction 30 minutes 4 ml blood Plain (Red) Upto 24 hours/ 2 80C *Note: If the Clot retraction is poor, it is kept in the water bath for 24 hours. 20 313 Euglobulin lysis 24 hours 2.7ml blood 3.2% citrated blood Upto 24 test (Light Blue) hours/ 2 80C 21 313 Factor XIII 24 hours 2.7ml blood 3.2% citrated blood Upto 24 (Light Blue) hours/ 2 80C
In case of Baby Collection/ Patient for whom blood collection is difficult, blood is collected in 1 ml K2 EDTA Microtainers using Syringe and needle.
Information to the Patients: All samples for routine hematological tests, random blood collection is being done.
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 30 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF CLINICAL PATHOLOGY
S Code Name of the Turn Specimen Anticoagulant Storage/ NO NO test around time required (vaccutainer Temperature tubes)
1 018 *Urine routine 1 hour 20 50 ml Urine Discarded on the analysis Sample. same day/ *Glucose Room temp *Albumin
*Acetone and acetoacetic acid *Bile salts *Bile pigments *Urobilinogen *Occult blood or hemoglobin *Leucocyte 2. 020 *Motion routine 3 hours at least 4ml (4 Discarded on the analysis cm3) same day/ Room temp 3. 030 *Motion routine 3 hours 3 samples on 3 Discarded on the analysis occult consecutive days. same day/ blood or at least 4ml (4 Room temp hemoglobin cm3)/Day 4. 028 Urine – Sugar, 1 hour 20 – 50 ml Urine Discarded on the Alb, Acetone sample same day/ Room temp 5. 029 Urine – LFT 2 ½ hours 20 – 50 ml Urine Discarded on the sample same day/ Room temp
* Test which are under Scope of NABL. For the FFA undergone patients Urine should be collected after 24 hours.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Mantoux test is being done in SN Main, JKCN & NSN Centers
1. Code Mantoux test 48 hours Reading will be 601 reporting taken after 48 time hours. Don’t (TAT) apply soap (or) water (till 48 hours)
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
OUT SOURCE TESTS
S Code Name of the test Turnaround Specimen Anti Storage Information to the NO NO time (TAT) required coagulant / Temp patients for specimens (vacutainer erature collected before tubes) 2.00pm 1 801 Serum Thyroid Profile: Next day 2 ml of serum Plain 2 80C Sample collection Free T3, Free T4, TSH done up to 10.00am every day (Fasting/ Random) 2 808 Anti Phospholipid 5 days 2 ml of serum Plain 2 80C Antibody (IgG & IgM) 3 809 CD4 Counts 2 days 2 ml of whole EDTA 2 80C blood 4 811 Protein C (Activity) 5 days 2 ml of platelet 3.2% 2 80C poor citrated Sodium plasma Citrate 5 812 Protein S (Activity) 5 days 2 ml of platelet 3.2% 2 80C poor citrated Sodium plasma Citrate 6 813 Vitamin B12 Next day 2 ml of serum Plain 2 80C Fasting 7 816 Folate RBC 3 days 2 ml of serum & Plain & 2 80C 2 ml of whole EDTA blood 8 845 Serum Iron, TIBC & 24 hrs 2 ml of serum Plain 2 80C Should not take % Saturation iron medication for 3 days. Blood collection done up to 10.00am everyday (Random) 9 820 Serum Uric Acid Next day 2 ml of serum Plain 2 80C 10 830 Serum Acid Next day 2 ml of serum Plain 2 80C Phosphatase 11 846 Cortisol Next day 2 ml of serum Plain 2 80C Blood collection done only at 8.00am (Fasting) & 4.00pm(Random) 12 850 Others (Specify) Refer Directory of Services Lister Metropolis Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 33 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
ACCEPTANCE AND REJECTION CRITERIA FOR COLLECTION AREA, HEMATOLOGY & CLINICAL PATHOLOGY
S.NO ACCEPTANCE CRITERIA REJECTION CRITERIA
1. Collection Area Properly labeled Collection Area – improperly labeled specimens. 1. Specimens not labeled 1. Full patient name and Date of birth 2. Specimens labeled with the incorrect patient 2. Patient identification number. identification 3. Date and time of collection 3. Specimens, that do not match the patient 4. Phlebotomist signature. information on the laboratory requisition.
2. Collection Area – Correct Specimen Collection Area – Improper Collection. Collection 1. Specimens collected with the improper 1. Specimens collection with proper preservative or anticoagulant Preservative or anticoagulant. 2. Quantity of specimens insufficient to perform 2. Correct volume testing 3. Specimen collection without any 3. Specimens which are hemolyzed, lipemic or hemolysis, lipemic or particulate contain particulate matter. Individual protocol must be matter reviewed. 4. Specimen without any 4. Specimens which are obviously or subsequently contamination prove to be contaminated.
3. Transportation of Specimens in 3 tier Delay in Transit to the laboratory: packing system. 1. Serum Specimens not separated from the clot and left at room temperature or refrigerated for a time, which exceeds the protocol for, the test requested. 2. Urine specimens left at room temperature for more than two hours. 3. Coagulation specimens more than four hours except for PT which is up to 24 hours. 4. Urine specimens for culture left at room temperature for more than two hours or refrigerated for more than 24 h . 4. Specimens collected by proper veni Inappropriate specimens: puncture site. 1. Specimens collected from intravenous tubing. 2. Specimens collected from heparin locks.
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 34 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
5. Specimen transportation with Specimens inappropriately transported to the laboratory: appropriate packing. 1. Specimens not in compliance with universal precaution, (e.g. Not Bagged) 2. Specimens leaking or grossly contaminated on the exterior portion of container. Note: Irretrievable specimens, such as Cerebral spinal fluid (CSF), operating room specimens, cord blood, biopsy or specimens taken prior to antibiotic therapy will not be discarded. 6. Hematology : A. Inadequate Specimens: 1. Lavender vaccutainers for hematology analysis with EDTA samples Properly mixed less than 2ml/1ml without any clot. 2. Blue vaccutainer for Coagulation studies which are less than 2.7ml/1ml. B. Clotted Specimens: The presence of clots in the vaccutainers upon visual inspection. C. Hemolysed samples: 1.Grossly haemolysed samples(EDTA) giving inaccurate results or unreadable blood films should be rejected 2.Hemolysed Citrate plasma should be rejected D. Aged specimens: 1.EDTA samples more than 24 hours old are rejected 2. Coagulation specimens more than 4 hours except for PT which is up to 24 hours. E. Test is not offered in Haematology laboratory.
7. Clinical Pathology: 1. Any Sample (Urine /Motion) brought after 1 h of 1. Urine Sufficient quantity in clean Collection. non sterile Container brought within 1 2. Insufficient Quantity. hour of Collection. 3. Specimen collected within 24 hrs after performing 2. Stool Sufficient quantity in clean fundus fluorescence angiogram. non sterile Container 4.Gross contamination with vaginal/anal secretions 3. Stool Samples brought within 1 If the samples are processed in known case of menstrual hour of Collection. or other unsatisfactory conditions or contaminations then the final report shall mention the nature of problem.
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 35 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DEPARTMENT OF
CLINICAL & SPECIAL
BIOCHEMISTRY
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 36 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF CLINICAL BIOCHEMISTRY
Specime Storage Lab/ Anti Reportable S. Name of the n of Ref/ coagulants interval Information to the No test required examined Code (vacutainer) (TAT) patients Blood - specimen volume / temp. 1. 102 Plasma Glucose 2 ml + Sodium 1 hour 24 hours F Fasting no caloric F/R/PP Urine to be Fluoride + /2 8°C intake for at least 8 collected Na 2 EDTA hrs. R 3 hours intake of food. PP Postprandial Blood should be collected 2 hrs after intake of food. 2. 103 Oral Glucose 2 ml + Sodium 3/4 hours 24 hours / First sample should be Tolerance test Urine to be Fluoride + 2 8°C collected in fasting (OGTT) collected Na 2 EDTA condition. 2nd sample 1 hour after 82.5gms glucose ingestion. 3rd sample 2hrs after 82.5grms glucose intake. 3. 104 Gestational 2ml + Sodium 4 hours 24 hours / First sample should be Diabetes Urine to be Fluoride + 2 8°C collected in fasting Mellitus (GDM) collected. Na 2 EDTA condition. 2nd sample 1 hour after glucose ingestion. 3rd sample 2hrs after 82.5 grms glucose intake. 4th samples 3 hrs after 100 gms glucose Intake.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
4. 105 Plasma 4 ml Heparin/plain 2 hours 24 hours / Urea/Creatinine 2 84°C 5. 106 Serum Cholesterol 4 ml Plain/Heparin 2 hours 24 hours / 8 – 12 Fasting 2 8°C 6. 4 ml plain 24 hours 24 hours / The test is out 820 Serum Uric acid 2 8°C sourced. 7. Plasma Sodium 4 ml Heparin/Plain One hour 24 hours / 108 and Potassium 2 8°C 8. Plasma Sodium, 4 ml Heparin/Plain One hour 24 hours / 109 Potasium, Chloride 2 8°C and Bicarbonate 9. Serum Calcium and 4 ml Plain 24 hours / 110 3 hours Phosphorus 2 8°C 10. 4 ml Plain/Heparin 24 hours / 113 Serum LFT 3 hours 2 8°C 11. Serum Bilirubin 4 ml plain 24 hours / 114 3 hours (Total/Direct) 2 8°C 12. Serum Total 4 ml Plain 3 hours 24 hours / 115 Protein and 2 8°C albumin 13. Serum Alkaline 4 ml Plain 24 hours / 116 3 hours Phosphatase 2 8°C 14. Serum Alanine 4 ml Plain 3 hours 24 hours / 117 amino transferase 2 8°C 15. Serum Aspartate 4 ml Plain 24 hours / 118 3 hours amino transferase 2 8°C 16. Lipid profile 4 ml Plain 3 hours 24 hours / Blood should be Total Cholesterol collected in fasting 123 2 8°C HDL cholesterol condition Triacylglycerol 17. 124 Plasma Triacyl 4 ml EDTA 3 hours 24 hours / Blood should be Glycerol (TGL) 2 8°C collected in fasting condition
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
18. 126 Angiotensin 4 ml Plain 24 hours 24 hours / For the FFA converting enzyme 20 °C undergone patients (ACE) blood should be collected after 24 hours. 19. 127 Urine 20ml Urine 8 hours Discarded Provide 3 urine Inborn errors of urine container on the containers with pinch metabolism same day of sodium azide. 1 for fasting and 2 for Random sample 20. 128 CSF– sugar, CSF 8 hours 24 hours/ Should be received as protein and chloride 1.0ml 2 8 °C early as possible. (Same day) 21. 122 Urine 50ml Urine 8 hours Discarded Urine containers Homocystinuria urine container on the should be provided screening same day. with pinch of sodium azide. Sample should be collected in fasting condition. 22. 151 HbA1c 2 ml EDTA Same day 24 hours Blood to be collected /4°C in fasting / Random condition 23. 152 Micro albumin 25ml Urine Same day 24 hours Urine to be collected in urine container. /4°C fasting condition./ (Sterile Random condition urine contain er)
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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TEST MASTER LIST
DEPARTMENT OF SPECIAL BIOCHEMISTRY
Temp./Sto Specimen rage of Specimen Anti Reportable Code Name of the Required examine Information to S. required intervals no Test Blood- d the patients NO ( manual) Coagulant Volume (TAT) specime n 1 131 Serum 4 ml plain 3 days 20o C/3 Should not take Vitamin A days vitamin A tablet, carrot not to be taken in the previous 24 hours. Blood should be collected in fasting condition. 2 138 Plasma 2 ml (fasting) EDTA 5 days 20o C/ 3 Blood should be Homocysteine days collected in fasting – plasma condition (ELISA) 3
4 121 Electrophoresi 5 ml urine 4ml plain 2 days 20o C/ 3 s for protein sample/1ml days serum/ CSF urine/CSF 5 122 Electrophoresi 4 ml plain 2 days 20o C/ 3 Fasting condition s for serum/ days Lipoprotein
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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6 125 Blood Pyruvate and 2 ml 4ml of 0.6M 2 days 20o C/ 3 Lactate blood per chloric days acid. 7 129 Blood Glucose 6 4 ml Heparin/ 2 days 24 hours Test is outsourced phosphate EDTA /4°C Dehydragenase 8 130 Serum Lactate 4 ml Plain 2 days 24 hours dehydrogenace /4°C
9 133 Paper 2 ml blood 2 ml Fluoride 2 days 20o C/ 3 Food + 2 glass of chromatography for in Fluoride blood NaF& Na2 days milk intake. after 2 Galactose/any sugars + EDTA hours blood to be 5 ml urine collected. 10 134 Paper 5 ml urine ACD 3 days 20o C/ 3 Blood should be chromatography for (fasting) 300 L days collected in fasting Ornithine / amino condition. acids 11 137 Serum amylase 4 ml 3 days 20o C/3 (plain) days 12 141 Serum Vitamin E 4 ml 3 days 20o C/3 (plain) days 13 142 Plasma vitamin C 4 ml Heparin 2 days 24 hours /4°C 14 143 Blood Glutathione 2 ml EDTA 3 days 20o C/3 days 15 144 Blood Glutathione 2 ml EDTA 3 days 20o C/3 Peroxidase days 16 145 Blood Superoxide 4 ml Heparin 3 days 20o C/3 dismutase days 17 146 Blood Thio Barbituric 2 ml EDTA 3 days 20o C/ 3 Acid Reactive days Substances
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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18 153 Trace elements 4 ml plain Plain 2 24 hours /4°C Call biochemistry 20days department before the collection to check the equipment if functioning. 19 112 4 ml Plain Plain Serum Ceruloplasmin 3 days 3 days /2 8°C
20 135 Amino acid profile – 2.0 ml ACD 7 days 7 days 20°C Blood should be plasma (Acid collected in fasting citrate condition dextrose) 300 L 21 136 Amino acid profile 5.0 ml Urine 7 days 7 days / Provided with pinch Urine container 20°C of sodium azide. Urine sample should be collected in fasting condition
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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ACCEPTANCE / REJECTION CRITERIA FOR BIOCHEMISTRY
Specimen rejection S.No Action to be taken Corrective action criteria
1 Unlabelled /wrongly If specimen obtained by non Record the problem in the labeled samples invasive procedure (Urine, sample rejection register and Sputum or throat swab) – document the corrective action Recollect the sample. taken If specimen is obtained by invasive procedure like needle aspiration, or if it is body fluids, then process after consulting with the referring physician. 2 If sample does not Do not Process. Inform the Record the problem in the match with the Test referring physician and ask for a sample rejection register and Request form sample rework document the corrective action taken 3 Improper/broken/ Do not Process. Inform the Record the problem in the Leaking Specimen referring physician and ask for a sample rejection register and container sample rework document the corrective action taken 4 Prolonged transport Do not Process. Inform the Record the problem in the time beyond one hour referring physician and ask for a sample rejection register and for all samples sample rework document the corrective action taken
5 Specimen unsuitable for Do not Process. Inform the Record the problem in the the test requested referring physician and ask for a sample rejection register and sample rework document the corrective action taken
6 Insufficient sample Process as much as possible. Record the problem in the volume Inform the referring physician and sample rejection register and ask for a sample rework. document the corrective action taken
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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7 Do not Process. Inform the Record the problem in the Specimen collected in referring physician and ask for a sample rejection register and inappropriate or wrong sample rework document the corrective action vacutainer under If precious sample process with taken inappropriate conditions approval from authorized
as required by the tests signatory with comments in
requested report.
8 Sample collected from a Do not process. Inform the Record the problem in the patient who had not referring physician and ask for a sample rejection register and fulfilled the preparation sample rework. document the corrective action criteria for test taken requested 9 Contamination of Do not process. Inform the Record the problem in the specimen collected referring physician and ask for a sample rejection register and Eg: IV line sample sample rework. document the corrective action collection blood taken Urine contamination with vaginal secretion or fecal matter 10 Hemolysed samples will Do no process. Inform the be rejected based on referring physician and ask for a the degree of hemolysis sample rework. and the test requested. Also follow the below enumerated Lipemia : criteria on need as per case by Record the problem in the TGL >600mg/dl case basis and tests requested. sample rejection register and Highly icteric sample If precious sample or in special document the corrective action cases with partial hemolysis after taken approval by authorized signatory sample can be processed with comment in the report.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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Effects of Hemolysis/ Lipemic /icteric interference on Biochemical parameters
Hemolysed samples: Lipemic samples: Highly icteric samples:
Hemolysis interference is With slight lipemia to marked Falsely decreased: approximately linearly dependent lipemia: >= 6 total protein and on Falsely increased: Triglyceride and in very rare the final concentration of free Hb Direct bilirubin and TIBC instances of highly icteric in the specimen. Falsely decreased: samples cholesterol, creatinine, • It generates a consistent trend sodium, chloride and GGT, Uric acid are also affected. towards overestimation of: comparatively less effect Unknown or increased: – ALT & AST potassium Fructosamine is affected in slight – Creatinine Unknown sometimes increased to high icteric index only in very – Creatine kinase (CK) or decreased: high icteric Direct bilirubin and – Iron ALT, AST, Albumin, Calcium, CK, phosphate is affected. – LDH Cholinesterase, BUN, – Lipase Phosphate, Total Bilirubin – Magnesium – Phosphorus – Potassium – Urea • It generates a consistent trend towards underestimation of: – Albumin – ALP – Chloride & Sodium – GGT – Glucose • Clinically meaningful variations of AST, chloride, LDH, potassium and sodium were observed in specimens with mild hemolysis (free Hb ~60 mg/dL).
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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DEPARTMENT OF
MICROBIOLOGY &
SEROLOGY
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 46 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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TEST MASTER LIST
DEPARTMENT OF MICROBIOLOGY
S.No Test Name of Name of clinical Volume Criteria for Criteria Turn Temper code Test specimen Criteria Acceptance for around ature of of clinical Rejection time* Storage specimen of Clinical specimen 1 401 Grams Conjunctival Swab One Swab Not Not 6 8 2 8°C Stain Stick for all applicable applicable hours stains Sputum 3 5ml Freshly Contamin 6 8 2 8°C collected ated with hours sputum saliva Throat Swab One Swab Not Not 6 8 2 8°C Stick for all applicable applicable hours stains Ocular specimens, Not applicable Not Not 6 8 2 8°C non ocular applicable applicable hours specimens and biopsy (as described in page 5) 2 402 KOH / Conjunctival Swab One Swab Not Not 6 8 2 8°C Calcofluor Stick for all applicable applicable hours Stain stains Sputum 3 5ml Freshly Contamin 6 8 2 8°C collected ated with hours sputum saliva Throat Swab One Swab Not Not 6 8 2 8°C Stick for all applicable applicable hours stains Stool One teaspoon Not Not 6 8 2 8°C ful/5ml applicable applicable hours Ocular specimens, Not applicable Not Not 6 8 2 8°C non ocular applicable applicable hours specimens and biopsy (as described in page 5)
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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403 Giemsa Conjunctival Swab One Swab Not Not 6 8 2 8°C Stain Stick for all applicable applicable hours stains Throat Swab One Swab Not Not 6 8 2 8°C Stick for all applicable applicable hours stains Ocular specimens, non Not applicable Not Not 6 8 2 8°C ocular specimens and applicable applicable hours biopsy (as described in page 5) 4 404 AFB Sputum 3 5ml Freshly Contamin 6 8 2 8°C Stain collected ated with hours sputum saliva Throat Swab One Swab Not Not 6 8 2 8°C Stick for all applicable applicable hours stains Stool One teaspoon Not Not 6 8 2 8°C full /5ml applicable applicable hours Ocular specimens, Not applicable Not Not 6 8 2 8°C non ocular specimens applicable applicable hours and biopsy (as described in page 5.) 5 411 Bacterial Conjunctival Swab One Swab To be If collected 48 – 56 2 8°C culture Stick for collected in a hours inoculation to and unsterile all plates transported container in HBSS Sputum 3 5ml Freshly Contamin 48 – 56 2 8°C collected ated with hours sputum in a saliva sterile container Throat Swab One Swab To be If collected 48 – 56 2 8°C Stick for collected in in a hours inoculation to a sterile unsterile all plates container container Stool One teaspoon To be If collected 48 – 56 2 8°C ful/5ml collected in in a hours a sterile unsterile container container
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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Urine 5 10ml Mid stream If collected 48 – 56 2 8°C urine to be in a hours collected in unsterile a sterile container, container if the patient is on antibiotic therapy Ocular specimens, Not applicable Not Not 48 – 56 2 8°C non ocular specimens applicable applicable hours and biopsy (as described in page 5) other than blood 6 412 Fungal Conjunctival Swab One Swab for To be If collected culture inoculation to collected in in a 12 days 2 8°C all plates a sterile unsterile container container Sputum 3 5ml for Freshly Contamin 12 days 2 8°C inoculation to collected ated with all plates sputum in a saliva sterile container Throat Swab One Swab for To be If collected 12 days 2 8°C inoculation to collected in in a all plates a sterile unsterile container container Ocular specimens, Not applicable Not Not 12 days 2 8°C non ocular specimens applicable applicable and biopsy (as described in page 5) other than blood 7 414 Acantha Corneal scraping, Not applicable Not Not 10 days 2 8°C moeba Corneal biopsy, applicable applicable culture Contact lens solution, Contact lens
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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Ocular specimens: Conjunctival swab, Conjunctival scraping, Corneal scraping, Corneal button, Aqueous aspirate, Vitreous aspirate, Lens aspirate, Infected Suture, Infected buckle, Donor corneal rim (DCR), Iris tissue, Intra ocular lens (IOL), Contact lens, Capsular bag, Eviscerated material, Canalicular pus and orbital pus and any other ocular biopsy tissue, Sub retinal mass, and Scleral nodule, Lasik flap and Orbital biopsy. Non-ocular and other clinical specimens: Throat swab, Sputum, Pus, Urine, Cerebro spinal fluid (CSF), Biopsy*, Nail clippings, Hair, Scrapings from genital lesions, Amniotic fluid, Bronchial wash, Ascitic fluid, Pleural fluid, Bronchoalveolar Lavage, Tracheal aspirate, Nasopharyngeal aspirate, Vesicle fluid, Synovial fluid, CAPD, Skin scraping, Gastric washing. *Biopsy – Bone marrow, Bone, Lymph node, Fine needle aspiration biopsy, FNAB, Sub retinal mass ,Abscess fluid, Gastric biopsy, Lung biopsy, Liver biopsy, Brain biopsy, Ileo caecal biopsy, Granuloma, Endometrial biopsy, Skin nodule or any other biopsy specimens.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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TEST MASTER LIST
DEPARTMENT OF SEROLOGY
S.N Test Name of Test Sample to be Volume Criteria for Criteria for Turn Temper o code collected Criteriaª Acceptance of Rejection around ature of ( Vacutainer) serum/plasma of time* Storag serum/ e plasma 1. 202 Non Treponemal Plain blood/ 2 4 ml Free from RBCs, Grossly 2 3 days # 2 8°C (RPR) and EDTA, and of required Haemolys treponemal (TPHA) Heparinized/ volume ed/ antibodies citrated contamina Blood ted/lipemic 2. 203 Rheumatoid Plain blood 2 4 ml Free from RBCs Grossly 2 3 days # 2 8 °C arthritis (RA) and of required Haemolys Factor volume ed/ (Nephlometry) contamina ted/lipemic 3. 218 C Reactive Plain blood 2 4 ml Free from RBCs Grossly 2 3 days# 2 8 °C protein (CRP) and of required Haemolys (Nephlometry) volume ed/ contamina ted/lipemic 4. 204 Brucella Plain blood 2 4 ml Free from RBCs Grossly 2 3 days # 2 8 °C Agglutination Test and of required Haemolys @ volume ed/ contamina ted/lipemic 5. 317 HBs Ag, Antibodies Plain blood/ 2 4 ml Free from RBCs Grossly 24 2 8 °C to HCV, Non EDTA, and Haemolys 36hours$ Treponemal (RPR) Heparinized/ of required ed/ and Treponemal Citrated volume contamina (TPHA) antibodies Blood ted/lipemic
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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6. 220 Antibodies to Plain blood/ 2 4 ml Free from RBCs Grossly 24 36 2 8 °C HIV 1 and HIV 2 EDTA, and of required Haemolys hours$ and HIV 1 p24 Heparinized/ volume ed/ antigen citrated contamina ( Screening and Blood ted/lipemic ELISA) 7. 221 Western Blot for Plain blood/ 2 4 ml Free from RBCs Grossly Three 2 8 °C antibodies to EDTA, and of required Haemolys months or HIV 1 and HIV 2 Heparinized/ volume ed/ three citrated contamina samples Blood ted/lipemic positive for HIV1 or HIV2 Abs 8. 222 Hepatitis B surface Plain blood/ 2 4 ml Free from RBCs Grossly 24 36 2 8 °C antigen ( HBsAg ) EDTA, and Haemolys hours$ ( Screening and Heparinized/ of required ed/ ELISA) citrated volume contamina Blood ted/lipemic 9. 260 Antibodies to Plain blood/ 2 4 ml Free from RBCs Grossly 3 days or 2 8 °C HBsAg EDTA, and Haemolys two Heparinized/ of required ed/ samples Citrated volume contamina which ever Blood ted/lipemic is earlier 10. 223. Antibodies to Plain blood/ 2 4 ml Free from RBCs Grossly 24 2 8 °C Hepatitis C virus EDTA, and Haemolys 36hours$ (HCV) Heparinized/ of required ed/ ( Screening and citrated volume contamina ELISA) Blood ted/lipemic 11. 206 Anti nuclear Plain blood 2 4 ml Free from RBCs Grossly 2 3 days # 2 8 °C antibody and of required Haemolys (Fluorescent and volume ed/ ELISA) contamina ted/lipemic
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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12. 234 Plain blood/ 2 4 ml Free from RBCs Grossly 2 3 days# 2 8 °C IgG antibodies to EDTA, and Haemolys cANCA Heparinized/ of required ed/ (Proteinase3) Citrated volume contamina plasma ted/lipemic 13. 235 Plain blood/ 2 4ml Free from RBCs Grossly 2 3 days# 2 8 °C IgG antibodies to EDTA/ and of required Haemolys pANCA Heparin/ volume ed/ (Myeloperoxidase) Citrated contamina plasma ted/lipemic 14. 233 Antibodies to Plain blood/ 2 4 ml Plain blood to be Grossly 3 days or 20 °C Aquaporin 4 EDTA, separated and Haemolys two (NMO – IgG) Heparinized/ transported in ice ed/ samples ELISA Citrated contamina which ever Blood ted/lipemic is earlier
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TEST MASTER LIST
DEPARTMENT OF MICROBIOLOGY AND SEROLOGY LABORATORY (NOT UNDER NABL SCOPE)
S. No Test Name of Test Sample to be Volume Criteria for Criteria for Turn Tempera code collected Criteriaª Acceptance of Rejection of around ture ( Vacutainer) serum/plasma serum/plasma time* of Storage 1 205 Widal Test Plain blood 2 4 ml Free from RBCs Grossly 24 30 2 8 °C and of required Haemolysed/ hours volume contaminated/ lipemic 2 217 Anti streptolysin O Plain blood 2 4 ml Free from RBCs Grossly 2 3 2 8 °C (ASO) (Latex) and of required Haemolysed/ days# volume contaminated/ lipemic 3 453 HLA B27 EDTA blood 2 3 ml Not applicable Not applicable 2 3 2 8 °C days# 4 219 Serum Lysozyme Plain blood 2 4ml Free from RBCs Grossly 2 3 2 8 °C assay and of required Haemolysed/ days# volume contaminated/ lipemic 5 434 Nigrosin stain Cerebro Not Not applicable Not applicable Same 22 Spinal Fluid applicable day 26°C (CSF) 6 430 pp65 antigen assay EDTA blood 2 3 ml Not applicable Not applicable 24 36 20°C for CMV ( IF hours technique) 7 458 Bacterial culture 10 ml for Collected and Collected in a 14 Not Blood culture by Blood adults inoculated vacutainer days applicabl BACTEC 5 ml for immediately with or e ( Fungi, Aerobic children aseptically without and Anaerobic below 5 anticoagulant bacteria) yrs
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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8 415 Mycobacterial Sputum 3 5ml Freshly collected Contamin 42 days 2 8°C culture in a sterile ated with ( Micro MIGIT container saliva Method) Ocular Not Collected in a Not 42 days 2 8°C specimens, applicable sterile container collected non ocular in a sterile specimens container, and biopsy leaky (as container described in page 5) other than blood 9 456 Antibiotic ( First Isolate of Not Not applicable Not 15 days 22 line drugs SIREP) Mycobacteriu applicable applicable 26°C sensitivity for m Mycobacterium tuberculosis tuberculosis (BACTEC method) 10 407 Imm. fluor Stain for Throat Swab One Swab Not applicable Not 6 8 2 8°C ( HSV) Stick for applicable hours** all stains Conjunctival One Swab Not applicable Not 6 8 2 8°C Swab Stick for applicable hours** all stains Ocular Not Not applicable Not 6 8 2 8°C specimens, applicable applicable hours** non ocular specimens and biopsy (as described above) 11 431 Rubella virus Amniotic isolation fluid/ whole 1 2 ml Not applicable Not 10 days 2 8°C blood in a applicable plain vacutainer
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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12 416 HSV isolation Conjunctival One Swab To be collected in Unsterile 10 days 4°C Swab Stick MEM/HBSS container/l eaking container Throat Swab One Swab To be collected in Unsterile 10 days 4°C Stick MEM/HBSS container/l eaking container Corneal Not Sterile Unsterile 10 days 2 8°C button applicable container/leak container/l proof container eaking container Skin scraping Not To be collected in Unsterile 10 days 4°C applicable MEM/HBSS container/l eaking container 13 419 Adenovirus Conjunctival One Swab To be collected in Unsterile 10 days 4°C Isolation Swab Stick MEM/HBSS container/l eaking container Throat Swab One Swab To be collected in Unsterile 10 days 4°C Stick MEM/HBSS container/l eaking container Nasopharyng 1 2ml Collected in a Collected 10 days 2 8°C eal aspirate sterile container in a and transported unsterile in ice with in 1 2 container hours and transporte d after 2 hours of collection
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14 413 Chlamydia Conjunctival One Swab To be If 10 days 2 8°C trachomatis Swab Stick collected in transported culture MEM/HBSS the swab at ( CT) without room antibiotics temperature and If collected in MEM containing antibiotics Corneal Not To be If 10 days 2 8°C scraping, applicable collected in transported Conjunctival MEM/HBSS the swab at scraping, without room genital swab antibiotics temperature and If collected in MEM containing antibiotics Nasopharyng 1 2ml Collected in a Collected in 10 days 2 8°C eal aspirate sterile un sterile container and container and transported in transported ice with in1 2 after 2 hours hours of collection 15 207 Antibodies to Plain blood/ 2 4ml Free from Grossly 2 3 2 8 °C Toxoplasma gondii EDTA/ RBCs and of Haemolysed/ days# ( IgG and IgM) Heparin/ required contaminated Citrated volume / plasma lipemic 16 210 Antibodies to Plain blood 2 4 ml Free from Grossly 2 3 2 8 °C Rubella virus ( IgG RBCs and of Haemolysed/ days# and IgM) required contaminated volume / lipemic
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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17 211 Antibodies to CMV Plain blood 2 4 ml Free from RBCs Grossly 2 3 2 8 °C ( IgG and IgM) and of required Haemolys days# volume ed/ contamina ted/ lipemic 18 212 Antibodies to HSV Plain blood 2 4 ml Free from RBCs Grossly 2 3 2 8 °C ( IgG and IgM) and of required Haemolys days# volume ed/ contamina ted/ lipemic 19 213 Antibodies to EBV Plain blood 2 4 ml Free from RBCs Grossly 2 3 2 8 °C viral capsid antigen and of required Haemolys days# ( VCA) (IgG and volume ed/ IgM) contamina ted/ lipemic 20 263 QuantiFERON – Blood – Use 2 ml Collected in only Collected After 8 22 TB test Platinum only BD BD vacutainer in ordinary samples 26°C IGRA test vacutainer heparin tubes anticoagul are heparin tubes provided with the ated collected provided with kit vacutainer . Report the kit. will be generate d within 10 15 days 21 420 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C Mycobacterium applicable hours tuberclosis Ocular Not Collected in a Not 42 days 20°C (M. tb) specimens, applicable sterile container collected non ocular in a sterile specimens container, and biopsy leaky (as container described in page 5) other than blood
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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22 421 PCR for Cytomegalo EDTA blood 2 3 ml Not applicable Not 24 36 20°C virus (CMV) applicable hours 23 422 PCR for Herpes EDTA blood/ 2 3 ml Not applicable Not 24 36 20°C Simplex Virus (HSV) CSF applicable hours 24 423 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C Varicella Zoster Virus applicable hours (VZV) 25 424 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C Adeno Virus applicable hours 26 425 PCR for Chlamydia EDTA blood 2 3 ml Not applicable Not 24 36 20°C trachomatis applicable hours 27 451 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C Chlamydophila applicable hours pneumoniae 28 426 PCR for Ac tap, 0.05 0.1ml Collected in a Not 24 36 20°C Eubacterial Vitreous 0.05 0.3ml sterile container collected hours genome aspirate in a sterile container, leaky container 29 427 PCR for Pan fungal Ac tap, 0.05 0.1ml Collected in a Not 24 36 20°C genome Vitreous 0.05 0.3ml sterile container collected hours aspirate in a sterile container, leaky container 30 428 PCR for Ac tap/ 0.05 0.1ml Collected in a Not 24 36 20°C Propionibacterium Vitreous 0.05 0.3ml sterile container collected hours acnes aspirate in a sterile container, leaky container 31 429 PCR for EDTA blood/ 2 3 ml Collected in a Not 24 36 20°C Toxoplasma gondii Ac tap / 0.05 0.1ml sterile container collected hours Vitreous 0.05 0.3ml in a sterile aspirate/ 0.5 1ml container, CSF leaky container
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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32 452 RT PCR for Blood /DEPC 2 3 ml DEPC treated vial Anticoagul 24 36 20°C Rubella Virus treated vial ated hours sample and containers without DEPC 33 459 Real time PCR for EDTA blood 2 3ml DEPC treated vial Sample 24 36 20°C HIV 1 or plain and hours blood/ containers DEPC without treated vial DEPC treatment 34 460 Real Time PCR Blood /DEPC 2 3 ml DEPC treated vial Container 24 36 20°C for Chickungunya treated vial s without hours virus DEPC treatment 35 461 Real time PCR for EDTA blood 2 3 ml Not applicable Blood 24 36 20°C CMV collected hours in unsterile / leaking vial 36 462 Real time PCR for EDTA blood 2 3 ml Not applicable Blood 24 36 20°C HSV collected hours in unsterile / leaking vial 37 463 Real time PCR for EDTA 2 3 ml / Not applicable Blood 24 36 20°C M.tuberculosis blood/Ac 100 200ul collected hours tap/Vitreous in aspirate unsterile / leaking vial 38 464 Real time PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C Hepatitis B Virus applicable hours 39 465 Real time PCR for EDTA 2 3 ml Not applicable Not 24 36 20°C Hepatitis C virus blood/DEPC applicable hours treated vial Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 60 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
40 473 PCR for Leptospira EDTA blood/ 2 3 ml Collected in a Not 24 36 20°C interrogans Ac tap/ 0.05 0.1ml sterile container collected hours Vitreous 0.05 0.3ml in a sterile aspirate container, leaky container 41 474 PCR for EDTA blood Collected in a Not 24 36 20°C Salmonella typhi 2 3ml sterile container collected hours in a sterile container, leaky container 42 486 PCR for Broncho 2 3 ml Collected in a Not 24 36 20°C Pneumocystis alveolar sterile container collected hours jirovecii lavage / in a sterile Respiratory container, secretions leaky container 43 457 Imm. Fluor. Stain Fresh tissue Not Not applicable Not Not Not for pemphigus frozen applicable applicable applicabl applica sections e ble 44 467 PCR based DNA Any Not Collected in a Not 24 36 20°C Sequencing unidentifiable applicable sterile container collected hours bacterial/fung in a sterile al isolate for container, the leaky identification container of species level 45 471 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C M.fortuitum applicable hours Ocular Not Collected in a Not 42 days 20°C specimens, applicable sterile container collected non ocular in a sterile specimens and container, biopsy (as leaky described in container page 5) other than blood
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Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
46 472 PCR for EDTA blood 2 3 ml Not applicable Not 24 36 20°C M.chelonae applicable hours Ocular Not Collected in a Not 42 days 20°C specimens, applicable sterile container collected non ocular in a sterile specimens container, and biopsy leaky (as container described in page 5) other than blood 47 487 Reverse EDTA 2 3 ml / Not applicable Blood 24 36 20°C transcriptase PCR blood/Ac 100 200ul collected hours for M.tb tap/Vitreous in aspirate unsterile / leaking vial
*: Time taken from the receipt of specimen(s) in the Microbiology and Serology department to the time, soft copy of the reports will be available in the HMS format of the department # : Serology days: Tuesday/Thursday/Saturday: Samples received in the Microbiology department till 10.00am on the day of testing will be included and the report will be generated by 5.30 pm on the day of testing. @: Antibodies to Brucella is tested by tube agglutination test and the report will be ready by 11.00am, of the next working day (i.e. Wednesday/Friday/Monday) a: volume of blood for patients < 1 yr is 1.0-1.5ml
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Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
ACCEPTANCE / REJECTION CRITERIA FOR RECEIVING SPECIMEN AT SNSC LAB – MICROBIOLOGY AND SEROLOGY
Acceptance Criteria Rejection Criteria
1. 1.Swabs submitted for culture not identified its source Microbiology Collections: 2. Improper transport: Proper Collection and Transportation a. Urine specimens for culture left at room temperature for more than two hours or refrigerated for more than 24 hours. b. Anaerobic cultures not transported in an anaerobic environment.
2. All clinical specimen collected in sterile Culture specimen received in unsterile container for microbiology tests containers/non laboratory containers as evidenced by contamination of containers, leaking containers and containers with foreign material. 3. Clinical specimen transported in appropriate Samples which are not sufficient / Single swab transport medium for tests requested. submitted for multiple requests (for eg.direct smear study and culture for aerobic and anaerobic bacteria fungus and Mycobacterium tuberculosis./isolation of viruses etc ) 4. Samples collected before initiation of antibiotic Samples collected after initiation of antibiotic therapy. therapy.
5. Mid stream urine samples for pyogenic bacterial Urine samples (for pyogenic bacterial culture) collected culture collected with aseptic precautions and immediately after performing fundus fluorescence transported within half an hour to laboratory angiogram. Twenty four hour specimen collections for pyogenic urine culture 6. Sputum sample should be examined by Grams Sputum sample should be examined by Grams stain stain and score of leucocytes and squamous and score of leucocytes and squamous epithelial cells epithelial cells to be determined as in procedure to be determined as in procedure N of manual. The N of manual. The sample grouped under 4,5,6 sample grouped under 1,2,3 grade is not accepted for grade is accepted for testing. testing.
Note: If the specimens are sent for both Microbiological as well as Histopathological investigation, the specimen would be received in unfixed condition and half of the specimen should be sent to Histopathology lab from Microbiology laboratory.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Acceptance Criteria Rejection Criteria Serum samples that are clear without lysis or lipemic Serum samples that are lysed, serum that look lipemic and turbid with bacterial growth. Serum sample sufficient for test requested Serum sample insufficient for test requested
Primary /Secondary sample as given in table 1 for respective Primary /Secondary sample not as given tests. in table 1 for respective tests.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DEPARTMENT OF
HISTOPATHOLOGY &
CYTOPATHOLOGY
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 65 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF HISTOPATHOLOGY
Specimen Turn over Fixative Used/Acceptance Code No Time Criteria 501A Eyeball 4 Days 10%Neutral buffered formalin 502A Exenterated specimen 4 Days 10%Neutral buffered formalin 503A Eviscerated specimen 4 Days 10%Neutral buffered formalin 504A Corneal button 4 Days 10%Neutral buffered formalin 505A Epiretinal membrane 4 Days 10% Neutral buffered formalin 506A Sub retinal membrane 4 Days 10%Neutral buffered formalin 507A Biopsy lid 4 Days 10% Neutral buffered formalin 508A Biopsy Conjunctiva 4 Days 10% Neutral buffered formalin 509A Biopsy Cornea 4 Days 10%Neutral buffered formalin 510A Biopsy Orbit 4 Days 10%Neutral buffered formalin 511A Iris tissue 4 Days 10%Neutral buffered formalin 550 Others Tissue and cell block of 4 Days 10%Neutral buffered formalin fluids 501B 511B Frozen section 30 Minutes Fresh unfixed tissue 524 Amyloid stain 1 Days Paraffin block/Paraffin sections 525 Mucin stain 1 Days Paraffin block/ Paraffin sections 526 Collagen stain 1 Days Paraffin block/ Paraffin sections 528 GMS stain 1 Days Paraffin block/ Paraffin sections 529 Gram stain 1 Days Paraffin block /Paraffin sections 530 Calcium stain 1 Days Paraffin block/ Paraffin sections 531 AFB stain 1 Days Paraffin block/ Paraffin sections 532 Oil O’red stain 1 Days Frozen section
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
533 Perls stain 1 Days Paraffin block/ Paraffin sections 537 Small sized specimen (3mm 5mm) 4 Days 10%Neutral buffered formalin 538 Medium Sized specimens (10mm) 4 Days 10%Neutral buffered formalin 539 Large sized specimens (>20mm) 4 Days 10%Neutral buffered formalin
TEST MASTER LIST
DEPARTMENT OF CYTOPATHOLOGY
Code No Specimen Turn over Time Fixative Used/Acceptance Criteria 512 Aqueous aspirate 3 Days Unfixed sample sent in cool pack /smears 513 Vitreous aspirate 3 Days Unfixed sample sent in cool pack /smears 514 Sub retinal fluid 3 Days Unfixed sample sent in cool pack /smears 515 Smear cytology lid 3 Days Unfixed sample sent in cool pack /smears 516 Smear cytology Conjunctiva 3 Days Unfixed sample sent in cool pack /smears 517 Smear cytology cornea 3 Days Unfixed sample sent in cool pack /smears IOL Cytology 3 Days 70%or 95% Alcohol 519 FNAC 3 Days Unfixed sample sent in cool pack /smears 524 Amyloid stain 1 Days Unfixed sample sent in cool pack /smears 525 Mucin stain 1 Days Unfixed sample sent in cool pack /smears 526 Collagen stain 1 Days Unfixed sample sent in cool pack /smears 528 GMS stain 1 Days Unfixed sample sent in cool pack /smears 529 Gram stain 1 Days Unfixed sample sent in cool pack /smears 530 Calcium stain 1 Days Unfixed sample sent in cool pack /smears 531 AFB stain 1 Days Unfixed sample sent in cool pack /smears 532 Oil O’red stain 1 Days Unfixed sample sent in cool pack /smears 533 Perls stain 1 Days Unfixed sample sent in cool pack /smears
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Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF HISTOPATHOLOGY & CYTOPATHOLOGY
Tests (Specimens from External patients) not under scope of NABL
Code No Specimen Turn over Fixative Used/Acceptance Criteria Time 537 Small sized Skin biopsies, Lymph 4 Days 10%Neutral buffered formalin Node biopsy, Any other tissue measuring 3mm 5mm 538 Medium sized Skin biopsies, Lymph 4 Days 10%Neutral buffered formalin Node biopsy, Any other tissue measuring 10mm
539 Large sized Skin biopsies, Lymph 4 Days 10%Neutral buffered formalin Node biopsy, Any other tissue measuring >20mm 522 Aspirates vireous, aqueous, CSF, 3 Days Unfixed sample sent in cool pack Pleural fluid, Bronchial alveolar lavage /smears and any other body fluids
524 536 All special stains 1 Days Paraffin block, Unfixed sample sent in cool pack /smears
537B 539B Frozen section 30 Minutes Unfixed sample sent in cool pack
Specimens for histopathology and cytopathology including the impression cytology smears are collected by the surgeons at operation theater/out patient department are directly received in person or by mail at SNSC lab Histopathology 3rd floor New Building (KNBIRVO block)
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Instructions to Surgeons/Asst. Surgeons/OT staff for sending specimens to Histopathology
Volume of Cytology Mandatory Size of Frozen Labels on Formalin to be (aspirates / Information In container section Bottle added fluids) Requisition form
1.Small BHIB 5 10 Formalin 1. No Required 1 .Name of the patient sized bottles ml Not Fixative specimen Required 2. Age/gender 2. To be sent
immediately 3. MRD No. 2.Medium Wide (if delayed,
sized mouthed sample must 4. The type of specimen Container 20 be sent with (50 100 ml 50 cool pack) specimen sent OD/OS capacity ml
5. Date and Time of collection of sample 3. Large Wide sized mouthed specimens Container 6. Surgeon’s name (100 200 ml 150 capacity 200 7. Adequate clinical ml summary with clinical diagnosis
NOTE: If the same sample required for Microbiology and Histopathology Investigations Send the sample in sterile container without formalin first to Microbiology along with histopathology requisition form and Information about sending specimen to histopathology.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
MAILING OF SPECIMENS:
The specimen collected should be placed in suitable sized containers (preferably plastic container), with adequate fixative. The lid of the container should be sealed with help of adhesive tape (preferable plaster) to prevent leaking of the fixative.
To avoid leakage, fixed tissue can be wrapped in gauze soaked in 10 % Neutral buffered formalin.
The container should bear the label with details like Name, Age/sex of the patient, specimen, written legibly in a permanent marker.
PRESERVATION OF THE SPECIMEN
a. Cornea 5 to 10 ml b. Eyeball 150 to 200 ml c. Exenterated specimen 500 ml d. Other biopsy material according to the size of the specimen
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
The specimen should be mailed to Sri Nathella Sampathu Chetty Clinical Laboratory – Histopathology Laboratory,3rd Floor, New Building, No:41,College Road, Nungambakkam,Chennai 600 006 with proper requisition from the surgeon providing the required details mentioned above along with the address for communication andE mail/ telephone no. /Fax.
The charges intimated should be sent as Demand Draft/Cheque/NEFT payable to Medical Research Foundation.
Tissue should be preserved immediately in the proper fixative in a suitable sized container as drying of the tissue causes artifacts.
For routine surgical specimens 10% neutral buffered formalin is used, 70 0r 95% alcohol for IOL’s, Approximate volume of the fixative required for different kinds of specimens are
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
ACCEPTANCE AND REJECTION CRITERIA
S. No. Acceptance Criteria Rejection Criteria 1. Sample fixed in Formalin will be Totally dried up specimen without accepted fixative over 24hrs of collection will be rejected .
2. Paraffin block, stained No clinical information will be rejected sections/smears will be accepted.
3. Unstained sections / Smears will be Sample less than 1 mm will be rejected accepted
Note: If the specimens are sent for both microbiological as well as histopathological investigation, the specimen would be received in unfixed condition.
ORDERING FROZEN SECTION:
Frozen section is usually done in the case of adenexal tumors. Histopathology lab shall be informed about the details of the surgery and the patient including information about the special precautions at least 6 hours prior to surgery for frozen section to keep the cryostat machine ready. A report of such study shall be made ready with in 30 to 40 minutes
Specimens sent for frozen sections should be unfixed. If the samples are sent from JKCN OT /other places, ensure that the specimens are sent in cool pack to prevent drying with requisition form with relevant details mentioned above.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
DEPARTMENT OF
CYTOGENETICS
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 73 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
TEST MASTER LIST
DEPARTMENT OF GENETICS & MOLECULAR BIOLOGY (NOT UNDER NABL SCOPE)
S Code Specimen Anticoagulant Storage / Name of the test No. No. required (Vaccutainer tubes) Temperature
1 701 Chromosomal Study (PBLC Method) 4ml blood Sodium Heparin 6 days / 2 8°C
2 712 Genomic DNA Extraction (Mini kit) 4ml blood Sodium Heparin/ACD 6 days / 2 8°C
3 713 Genomic DNA Extraction (Maxi kit) 8ml blood ACD 6 days / 2 8°C
4 714 CYP1B1 Gene Screening for Congenital Glaucoma 8ml blood ACD 6 days / 2 8°C
5 715 MYOC Gene Screening for Open Angle Glaucoma 4ml blood ACD 6 days / 2 8°C
6 720 Others (Specify) 4/8ml blood Sodium Heparin/ACD 6 days / 2 8°C
Screening the three Primary mitochondrial 6 days / 2 8°C 7 723 mutations for Leber´s Hereditary Optic Neuropathy 8ml blood ACD (LHON)
8 725 PAX6 Gene Screening for Aniridia 8ml blood ACD 6 days / 2 8°C
9 726 RS1 Gene Screening Retinoschisis 8ml blood ACD 6 days / 2 8°C
10 733 NDP Gene screening for Norrie Disease 8ml blood ACD 6 days / 2 8°C
11 734 Eye Panel testing using NGS 8ml blood ACD 6 days / 2 8°C
12 735 Rare disease Panel testing using NGS 8ml blood ACD 6 days / 2 8°C
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
SPECIMEN ACCEPTANCE & REJECTION CRITERIA FOR – CYTOGENETICS
ACCEPTANCE CRITERIA:
1. It is preferable not to collect the blood sample from patient under chemotherapy, immune suppressive drugs or affected by immune suppressive disease and septicemia. Appreciable cytogenetic analysis could not be guaranteed during such time as these conditions lower the mitotic index and culture may get contaminated during the incubation thus hindering the results. Blood samples are accepted from such patients after discontinuation of those medications. 2. It is a random blood collection, 4 ml of blood sample to be collected in sterile sodium heparin vacutainer. 3. Blood sample should be collected without any hemolysis and clot. 4. The details on the patient’s name, age, sex, diagnosis, referral doctor and date of collection should be provided along with the sample. Preferably a label that contains all these details should be stuck on the sample tube. 5. The sample should be sent immediately after the collection at room temperature along with the requisition form, also the history of the patient diagnosis should be mentioned. 6. Address to send the sample is as follows, SN ONGC Department Genetics & Molecular Biology Sankara Nethralaya 18/41, College Road, Nungambakkam Chennai 600006 Ph.: 044 28271616, 28279435 7. Cost for the cytogenetic test payment can be made either by cash at the Register counter (or) by a DD addressed to Medical Research Foundation, payable at Chennai. 8. Analysis request for specific chromosomal region, if any, should be mentioned with the request letter.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
9. In case of pediatric blood sample, 0.5ml is accepted but if there is any contamination in the blood sample, culture cannot be performed and hence money would be reimbursed accordingly.
REJECTION CRITERIA:
Samples are rejected if: 1. Received with hemolysis and clot. 2. Received more than 2 days from the day of blood collection. 3. Less than 1 ml (except for pediatric blood samples). 4. Not received in room temperature. Note: Blood is received from other centers if the test is requested from the customer, however, the sample should be received in proper condition as instructed.
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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SCOPE
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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SCOPE
Laboratory Sri Nathella Sampathu Chetty Clinical Laboratory (Unit of Medical Research Foundation), No. 41, College Road, Chennai, Tamil Nadu Accreditation ISO 15189: 2012 Standard
Certificate Number MC-2342 (in lieu of M-0094)
Validity 14.08.2017 to 13.08.2019
CLINICAL BIOCHEMISTRY
1. Plasma Glucose Hexokinase 1 500 mg/dL 2.5 2. Plasma Urea Urease 1.0 150 mg/dL 4.1 3. Plasma Creatinine Jaffe Kinetic 0.05 20 mg/dL 3.9 4. Plasma / Serum Total Cholesterol CHO/POD Enzymatic 50 600 mg/dL 4.7 5. Plasma / Serum Sodium ISE Direct 40 200 mmol /L 0.9 6. Plasma / Serum Potassium ISE Direct 1.5 15 mmol /L 1.2 7. Plasma / Serum Chloride ISE Direct 50 200 mmol/L 1.7 8. Serum Calcium, Total OCPC / End Point 5 – 15 mg/dL 2.9 9. Serum Inorganic Phosphomolybdate 0.5 9 mg/ dL 4.1 Phosphorus 10. Plasma / Serum Bilirubin, Total Diazo caffeine 0.1 25 mg/dL 9.5 11. Plasma / Serum Bilirubin, Direct Diazo 0.05 – 16.0 mg/dL 6.7 12. Plasma / Serum Total Protein Biuret 2 12 g/ dL 8.7 13. Plasma / Serum Albumin BCP dye binding method 0.6 8.0 g/ dL 3.3 14. Plasma / Serum Alkaline PNPP with AMP 10.0 1000 U/L 6.3 Phosphatase 15. Plasma / Serum Alanine amino IFCC with P5P 6.0 – 1000 U/L 3.6 Transferase 16. Plasma / Serum HDL Cholesterol Direct Enzymatic 3.0 – 150 mg/dL 3.9 17. Plasma/Serum Triglyceride Enzymatic 15 1000 mg/dL 3.8 (Fasting) 18. Plasma / Serum Aspartic amino IFCC with P5P 0 1000 U/L 6.0 Transferase 19. Serum Angiotensin FAPGG UV Kinetic 8 251 U/L 12.0 Converting Enzyme
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
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CLINICAL PATHOLOGY
1. URINE PH REFLECTANCE 5.0 – 9.0 NA PHOTOMETRY – DIP STICK – METHYL RED AND BROMOTHYMOL BLUE 2. URINE SPECIFIC GRAVITY REFLECTANCE 1.000 – 1.030 NA PHOTOMETRY – DIP STICK – BROMTHYMOL BLUE 3. URINE GLUCOSE BENEDICTS NEGATIVE TO ++++ NA QUALITATIVE METHOD 4. URINE GLUCOSE REFLECTANCE NEGATIVE TO ++++ NA PHOTOMETRY – DIP STICK GLUCOSE OXIDASE/ PEROXIDASE 5. URINE ALBUMIN SULPHO SALICYLIC ACID NEGATIVE TO ++++ NA METHOD 6. URINE ALBUMIN REFLECTANCE NEGATIVE TO ++++ NA PHOTOMETRY – DIP STICK – TETRABROMPHENOL BLUE 7. URINE KETONES ROTHERA’S TEST NEGATIVE TO +++ NA 8. URINE PH REFLECTANCE 5.0 – 9.0 NA PHOTOMETRY – DIP STICK – METHYL RED AND BROMOTHYMOL BLUE 9. URINE BILE SALTS HAY’S TEST QUALITATIVE NA 10. URINE BILE PIGMENTS FOUCHET’ S METHOD QUALITATIVE NA 11. URINE UROBILINOGEN EHRLICH ALDEHYDE QUALITATIVE NA METHOD 12. URINE UROBILINOGEN REFLECTANCE QUALITATIVE NA PHOTOMETRY – DIP STICK – EHRLICH REACTION 13. URINE OCCULT BLOOD STANDARD GUAIAC NEGATIVE TO +++ NA OR HEMOGLOBIN METHOD 14. URINE LEUCOCYTE REFLECTANCE NEGATIVE TO + + + NA Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 79 of 88 Prepared & Issued by: Approved by:
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PHOTOMETRY – DIP STICK ESTERASE 15. URINE BILIRUBIN REFLECTANCE NEGATIVE TO + + + NA PHOTOMETRY – DIP STICK – DIAZOTIZED DICHLOROANILINE 16. URINE NITRITE REFLECTANCE NEGATIVE NA PHOTOMETRY – DIP POSITIVE STICK – P ARSANILIC ACID 17. URINE MICROSCOPIC MICROSCOPY QUALITATIVE NA EXAMINATION 18. STOOL STOOL ROUTINE MACROSCOPIC / QUALITATIVE NA MICROSCOPIC 19. STOOL OCCULT BLOOD STANDARD GUAIAC QUALITATIVE NA OR HEMOGLOBIN METHOD
HAEMATOLOGY & IMMUNOHAEMATOLOGY
1. K2 EDTA TOTAL WHITE ELECTRICAL 0.0 – 400.0 X 103/µL 2.0 Whole Blood BLOOD CELL IMPEDANCE COUNT 2. K2 EDTA RED BLOOD CELL ELECTRICAL 0.0– 8.0 X 106/µL 1.0 WHOLE (RBC) COUNT IMPEDANCE BLOOD 3. K2 EDTA PLATELET COUNT ELECTRICAL 0.0 3000 X 103/µL 2.4 WHOLE IMPEDANCE BLOOD 4. K2 EDTA HEMOGLOBIN PHOTOMETRY 0.0 25.0 G/DL 0.9 WHOLE (CYANMETHEMOGLOBIN BLOOD METHOD) 5. K2 EDTA HAEMATOCRIT ELECTRICAL 2.0 – 67.0% 1.4 WHOLE IMPEDANCE BLOOD 6. K2 EDTA RED BLOOD CELL DERIVATION NA 1.2 WHOLE INDICES BLOOD MCV 7. K2 EDTA RED BLOOD CELL DERIVATION NA 1.4 WHOLE INDICES BLOOD MCH
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8. K2 EDTA RED BLOOD CELL DERIVATION NA 1.8 WHOLE INDICES BLOOD MCHC 9. K2 EDTA DIFFERENTIAL VCS TECHNOLOGY / WHOLE COUNT ACV TECHNOLOGY / BLOOD MICROSCOPY/ LEISHMAN STAIN NEUTROPHILS % VCS TECHNOLOGY / 1 – 100% 1.7 ACV TECHNOLOGY / MICROSCOPY/ LEISHMAN STAIN LYMPHOCYTES % VCS TECHNOLOGY / 1 – 100% 4.0 ACV TECHNOLOGY / MICROSCOPY/ LEISHMAN STAIN EOSINOPHILS % VCS TECHNOLOGY / 1 – 100% 12.0 ACV TECHNOLOGY / MICROSCOPY/ LEISHMAN STAIN MONOCYTES % VCS TECHNOLOGY / 1 – 100% 9.2 ACV TECHNOLOGY / MICROSCOPY/ LEISHMAN STAIN BASOPHILS % VCS TECHNOLOGY / 1 – 100% 67.1 ACV TECHNOLOGY / MICROSCOPY/ LEISHMAN STAIN 10. K2 EDTA ABSOLUTE VCS TECHNOLOGY / 0.00 400 X 103/ L 2.8 WHOLE NEUTROPHIL ACV TECHNOLOGY / BLOOD COUNT MICROSCOPY/ LEISHMAN STAIN 11. K2 EDTA ABSOLUTE VCS TECHNOLOGY / 0.00 400 X 103/ L 4.4 WHOLE LYMPHOCYTE ACV TECHNOLOGY / BLOOD COUNT MICROSCOPY/ LEISHMAN STAIN 12. K2 EDTA ABSOLUTE VCS TECHNOLOGY / 0.00 400 X 103/ L 9.2 WHOLE EOSINOPHIL ACV TECHNOLOGY / BLOOD COUNT MICROSCOPY/ LEISHMAN STAIN 13. K2 EDTA ABSOLUTE VCS TECHNOLOGY / 0.00 400 X 103/ L 9.4 WHOLE MONOCYTE ACV TECHNOLOGY / BLOOD COUNT MICROSCOPY/ LEISHMAN STAIN 14. K2 EDTA ABSOLUTE VCS TECHNOLOGY / 0.00 400 X 103/ L 67.4 WHOLE BASOPHIL COUNT ACV TECHNOLOGY / Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 81 of 88 Prepared & Issued by: Approved by:
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LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
BLOOD MICROSCOPY/ LEISHMAN STAIN 15. K2 EDTA PERIPHERAL MICROSCOPY/ QUALITATIVE NA WHOLE SMEAR STUDY LEISHMAN STAIN BLOOD 16. K2 EDTA RED CELL DERIVATION NA 1.9 WHOLE DISTRIBUTION BLOOD WIDTH (RDW)
17. K2 EDTA RETICULOCYTE % MICROSCOPY/ NA NA WHOLE NEW METHYLENE BLUE BLOOD STAIN 18. CITRATED ERYTHROCYTE AUTOMATED 0 – 140 MM 12.3 WHOLE BLOOD SEDIMENTATION SEDIMENTATION (4NC) RATE (ESR) (PRO- EVACUATED) 19. CITRATED PROTHROMBIN CLOTTING – 8.0 115 SECONDS 4.0 PLASMA TIME (PT) NEPHELOMETRY (3.2% SODIUM CITRATE VACCUTAINER TUBE) 20. CITRATED ACTIVATED CLOTTING – 5.5 113 3.2 PLASMA PARTIAL NEPHELOMETRY (3.2% ) THROMBOPLASTIN SODIUM TIME (APTT) CITRATE 21. THICK WET SMEAR FOR MICROSCOPY/1% QUALITATIVE NA MOUNT MICROFILARIA (MF) METHYLENE BLUE/ PREPARATION 22. CITRATED WET MOUNT MICROSCOPY/1% QUALITATIVE NA BLOOD PREPARATION FOR METHYLENE BLUE MICROFILARIA (MF) 23. K2 EDTA SMEAR FOR MICROSCOPY/ QUALITATIVE NA WHOLE MALARIAL LEISHMANN’S STAIN/ BLOOD PARASITE (MP) GIEMSA STAIN/ CARD METHOD 24. K2 EDTA SICKLE CELLS MICROSCOPY/ QUALITATIVE NA WHOLE SODIUM META BI BLOOD SULFITE 25. K2 EDTA BLOOD GROUPING SLIDE & TUBE QUALITATIVE NA WHOLE & AGGLUTINATION BLOOD RH TYPING METHOD (REVERSE & FORWARD) Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 82 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
MICROBIOLOGY & SEROLOGY
1. Any Clinical Gram’s stain Manual/Microscopy Qualitative NA Specimen / Tissue Biopsy / Body Fluids Received For Bacterial Investigations. 2. Any Clinical AFB stain Ziehl Neelsen stain/ Qualitative NA Specimen / Manual / Microscopy Tissue Biopsy / Body Fluids Received For Bacterial Investigations. 3. Any Clinical KOH Calcofluor Fluorescence stain/ Qualitative NA Specimen / white stain Manual/Microscopy Tissue Biopsy / Body Fluids Received For Bacterial Investigations. 4. Any Clinical Manual/Microscopy Qualitative NA Specimen / Giemsa stain Tissue Biopsy / Body Fluids Received For Bacterial Investigations. 5. Any Clinical Bacterial Culture & Culture –Aerobic/ Qualitative except for NA Specimen / sensitivity Anaerobic urine and Conjunctival Tissue Biopsy / Sensitivity for Aerobic swab which are semi Body Fluids bacteria Disc diffusion Quantitative. Received For Bacterial Investigations. 6. Any Clinical Fungal Culture Culture Aerobic Qualitative NA Specimen / Tissue Biopsy / Body Fluids Received For Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 83 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Bacterial Investigations. 7. Any Clinical Acanthamoeba Non nutrient agar over laid Qualitative NA Specimen / culture with E. coli Tissue Biopsy / Body Fluids Received For Bacterial Investigations. 8. Serum RAPID PLASMA SLIDE FLOCCULATION SEMI NA REAGIN QUANTITATIVE ( RPR) NEAT 1:32 9. Serum TREPONEMA MICRO SEMI NA PALLIDUM HAEMAGGLUTINATION QUANTITATIVE HEMAGGLUTINATI 1:80 1:1280 ON (TPHA) 10. Serum RHEUMATOID NEPHELOMETRY QUANTITATIVE 3.8 ARTHRITIS (<10.0 100.0IU/ML) FACTOR (RA FACTOR) 11. Serum C REACTIVE NEPHELOMETRY QUANTITATIVE 3.1 PROTEIN (<0.5 320MG/L)) (CRP) 12. Serum ANTIBODIES TO TUBE AGGLUTINATION SEMI NA BRUCELLA QUANTITATIVE 1:10 – 1:160 13. Serum ANTIBODIES TO IMMUNO FILTRATION QUALITATIVE NA HIV – 1 & 2 METHOD 14. Serum ANTIBODIES TO IMMUNO QUALITATIVE NA HIV – 1 & 2 CONCENTRATION METHOD 15. Serum ANTIBODIES TO ELISA QUALITATIVE 9.5 HIV – 1 & 2, HIV 1 P24 AG 16. Serum ANTIBODIES TO WESTERN BLOT QUALITATIVE NA HIV – 1 & 2 17. Serum HBS AG IMMUNO FILTRATION/ QUALITATIVE NA IMMUNOCHROMATOGRA PHY
18. Serum HBSAG ELISA QUALITATIVE 7.8 19. Serum ANTI HBS ELISA QUANTITATIVE 11.9
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 84 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
ANTIBODIES (10 1000 MIU/ML) 20. Serum Antibodies to HCV Immunofiltration method Qualitative NA 21. Serum Antibodies to HCV ELISA Qualitative 8.8 22. Serum Antinuclear antibody Indirect Immuno Semi Quantitative NA fluorescence 1:80 – 1:160 23. Serum Antinuclear antibody ELISA Qualitative 9.6 24. Serum Antibodies against ELISA Qualitative NA specific antigen ds DNA, Histones, ribosomal, P proteins , nRNP / Sm, SS A, SS B, Scl 70 25. Serum IgG antibodies to ELISA Quantitative 10.9 cANCA(Proteinase3) (2 – 200 RU/ml) 26. Serum IgG antibodies to ELISA Quantitative 10.2 pANCA(Myeloperoxi (2 – 200 RU/ml) dase) 27. Serum IgG Antibodies to Indirect Immuno Semi Quantitative NA Aquaporin 4 (NMO) Fluorescence
HISTOPATHOLOGY
1. SMALL SIZED GROSSING, LIGHT MICROSCOPY DESCRIPTIVE NA SPECIMENS PROCESSING, (3MM-5MM) PARAFFIN - OCULAR EMBEDDING, MICROTOMY, H&E STAINING CONVENTIONAL 2. MEDIUM GROSSING, LIGHT MICROSCOPY DESCRIPTIVE NA SIZED PROCESSING, SPECIMENS PARAFFIN (10MM) EMBEDDING, - OCULAR MICROTOMY, H&E STAINING CONVENTIONAL Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 85 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
3. LARGE SIZED GROSSING, LIGHT MICROSCOPY DESCRIPTIVE NA SPECIMENS PROCESSING, (> 20MM) PARAFFIN - OCULAR EMBEDDING, MICROTOMY, H&E STAINING CONVENTIONAL 4. CELL BLOCK PROCESSING, LIGHT MICROSCOPY DESCRIPTIVE NA MICROTOMY, H&E STAINING (CONVENTIONAL) 5. FROZEN GROSSING, LIGHT MICROSCOPY DESCRIPTIVE NA SECTION PROCESSING, CRYSECTIONING, STAINING, (H&E) (CONVENTIONAL) 6. OCULAR PERIODIC ACID LIGHT MICROSCOPY DESCRIPTIVE NA TISSUES, SCHIFF STAIN PARAFFIN ALCIAN BLUE LIGHT MICROSCOPY DESCRIPTIVE NA BLOCKS, (PH 2.5) UNSTAINED CONGO RED LIGHT MICROSCOPY DESCRIPTIVE NA SLIDES (BENHOLD’S METHOD) GOMORIS ONE LIGHT MICROSCOPY DESCRIPTIVE NA STEP TRICHROME GRAMS (BROWN LIGHT MICROSCOPY DESCRIPTIVE NA HOPPS METHOD) ACID FAST LIGHT MICROSCOPY DESCRIPTIVE NA BACILLI GROCOTT’S LIGHT MICROSCOPY DESCRIPTIVE NA METHANAMINE SILVER NITRATE (GMS) ALIZARIN RED LIGHT MICROSCOPY DESCRIPTIVE NA BLEACHING LIGHT MICROSCOPY DESCRIPTIVE NA PERL’S IRON LIGHT MICROSCOPY DESCRIPTIVE NA
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 86 of 88 Prepared & Issued by: Approved by:
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SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
CYTOPATHOLOGY
1. Aspirate- Cytologic Exam As Per SOP, DESCRIPTIVE NA Aqueous, Light Microscopy and Vitreous, Interpretation Sub Retinal Fluid, Cyst, Other Aspirates 2. Smear Imprint Smear As Per SOP, DESCRIPTIVE NA Cytology – Lid, Preparation and Light Microscopy and Cornea, Cytologic Interpretation Conjunctiva Examination 3. Intraocular Cytologic As Per SOP, DESCRIPTIVE NA Lens Examination Light Microscopy and (IOL) Cytology Interpretation
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 87 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS
SRI NATHELLA SAMPATHU CHETTY CLINICAL
LABORATORY (UNIT OF MEDICAL RESEARCH FOUNDATION) 2018 DIRECTORY OF SERVICES
Issue No : 1 Issue Date : 26.07.2018 Amend No : 0 Page 88 of 88 Prepared & Issued by: Approved by:
Quality Manager Management Representative Dr.N.Angaarkanni Ph.D Dr.SB.Vasanthi MBBS