1 To be published in Freshwater Biology (2021, in press) 2 3 Manuscript title 4 The value of quantitative environmental DNA analyses for the management of invasive and 5 endangered native fish. 6 Running title 7 Value of quantitative eDNA in management. 8 Keywords 9 eDNA; Species interactions; Conservation; Fishes; Quantitative PCR 10 Target journal 11 Freshwater Biology 12 Authors 13 Jack Rojahn1, 2*, Luke Pearce3, Dianne M. Gleeson1, 2, Richard P. Duncan1, Dean M. Gilligan4, Jonas 14 Bylemans1, 2, 5 15 16
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[email protected] 18 19 1Centre for Conservation, Ecology and Genetics, Institute for Applied Ecology, University of 20 Canberra, Canberra, ACT 2617, Australia; 2Centre for Invasive Species Solutions, University of 21 Canberra, Canberra, ACT 2617, Australia; 3NSW Department of Primary Industries – Fisheries, 1 22 Albury, NSW 2640, Australia, 4NSW Department of Primary Industries – Fisheries, PO Box 17, 23 Batemans Bay, NSW 2536, Australia, 5Department of Ecology and Evolution, Biophore, University 24 of Lausanne, Lausanne 1015, Switzerland 25 * Corresponding author 2 26 Abstract 27 1. Environmental DNA monitoring is a useful tool for species detection but its use to address 28 management questions remains scarce. One factor limiting the use of eDNA as routine monitoring 29 tool is uncertainty around the potential of eDNA data to estimate species abundance. While several 30 confounding factors limit the ability of eDNA data to estimate absolute abundances at large spatial 31 and temporal scales, eDNA data have the potential to estimate relative species abundances patterns 32 at smaller scales, and this information can assist management.