AFF1 Antibody Cat. No.: 22-565

AFF1 Antibody

Immunofluorescence analysis of U2OS cells using AFF1 antibody (22-565).

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids IMMUNOGEN: 1124-1218 of human AFF1 (NP_001160165.1).

TESTED APPLICATIONS: IF, IHC, WB

WB: ,1:500 - 1:2000

APPLICATIONS: IHC: ,1:50 - 1:200

IF: ,1:50 - 1:100

POSITIVE CONTROL: 1) SW480

2) BT474

October 2, 2021 1 https://www.prosci-inc.com/aff1-antibody-22-565.html PREDICTED MOLECULAR Observed: 135kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: AFF1

AFF1, AF4/FMR2 family, member 1, AF-4, AF4, AF4/FMR2 family member 1, FEL, PBM1, ALTERNATE NAMES: Protein FEL, MLLT2, Unknown, Protein AF-4, Proto-oncogene AF4

GENE ID: 4299

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N- terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E BACKGROUND: mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants.

ANTIBODIES FOR RESEARCH USE ONLY.

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October 2, 2021 2 https://www.prosci-inc.com/aff1-antibody-22-565.html