AFF1 Antibody Cat. No.: 22-565 AFF1 Antibody Immunofluorescence analysis of U2OS cells using AFF1 antibody (22-565). Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Rat Recombinant fusion protein containing a sequence corresponding to amino acids IMMUNOGEN: 1124-1218 of human AFF1 (NP_001160165.1). TESTED APPLICATIONS: IF, IHC, WB WB: ,1:500 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200 IF: ,1:50 - 1:100 POSITIVE CONTROL: 1) SW480 2) BT474 October 2, 2021 1 https://www.prosci-inc.com/aff1-antibody-22-565.html PREDICTED MOLECULAR Observed: 135kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: AFF1 AFF1, AF4/FMR2 family, member 1, AF-4, AF4, AF4/FMR2 family member 1, FEL, PBM1, ALTERNATE NAMES: Protein FEL, MLLT2, Unknown, Protein AF-4, Proto-oncogene AF4 GENE ID: 4299 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N- terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E BACKGROUND: mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 2 https://www.prosci-inc.com/aff1-antibody-22-565.html.