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WO 2017/007310 Al 12 January 2017 (12.01.2017) P O P C T

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WO 2017/007310 Al 12 January 2017 (12.01.2017) P O P C T (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2017/007310 Al 12 January 2017 (12.01.2017) P O P C T (51) International Patent Classification: AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, A01K 67/033 (2006.01) BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (21) International Application Number: HN, HR, HU, ID, IL, EST, IR, IS, JP, KE, KG, KN, KP, KR, PCT/NL20 16/050480 KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, (22) International Filing Date: MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, 6 July 2016 (06.07.2016) PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, (25) Filing Language: English TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (26) Publication Language: English (84) Designated States (unless otherwise indicated, for every (30) Priority Data: kind of regional protection available): ARIPO (BW, GH, 2015 124 ' July 2015 (09.07.2015) N L GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (71) Applicant: PROTI-FARM R & D B.V. [NL/NL]; Harder- TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, wijkerweg 141, 3852 A B Ermelo (NL). DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventor: CALIS, Johannes Ignatius Maria; 270, Oude SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, Telgterweg, 3853 PK Ermelo (NL). GW, KM, ML, MR, NE, SN, TD, TG). (74) Agent: DE GRAVE-WOLTERINK, I.J.C.M.E.; P.O. Published: Box 3241, 2280 GE Rijswijk (NL). — with international search report (Art. 21(3)) (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, (54) Title: INSECT BREEDING FACILITY AND METHOD FOR THE INDUSTRIAL SCALE PRODUCTION OF MATURE LARVAE FROM EGG-LAYING MOTHERS □ 4 1 © Fig.l o o (57) Abstract: The present invention relates to an insect breeding facility and to a method for the industrial scale production of ma¬ ture larvae from egg-laying mothers, which mature larvae serve as protein rich food. The insect breeding facility comprises a spawn¬ ing area with a number of spawning containers, wherein at least one spawn structure is provided in each spawning container. In the o spawning area the egg-laying mothers spawn their eggs in spawn structures, and insect food is provided to the spawning containers. The insect breeding facility further comprises a hatch area with a number of hatching containers, which are adapted to receive the eggs, larvae food and to allow harvesting of mature larvae. Periodically, a batch of spawn structures is removed from the spawning containers and replaced with new spawn structures, and the eggs of the batches of removed spawn structures are transported into a hatching containers. Title: Insect breeding facility and method for the industrial scale production of mature larvae from egg-laying mothers The present invention relates to an insect breeding facility and to a method for the industrial scale production of mature larvae from egg-laying mothers, e.g. beetles, which mature larvae serve as protein rich food. Beetles are a group of insects which are biologically classified in the order Coleoptera and which are holometabolous insects, i.e. including four life stages - as an embryo or egg, a larva, a pupa, and an imago or adult. The first stage is from the fertilization of the egg inside the mother beetle until the embryo hatches. The insect starts as a single cell and then develops into the larval form before it hatches. The second stage lasts from hatching or birth until the larva pupates. In most species this mobile stage is worm-like in form, and these larvae are thus frequently referred to as "worms". The third stage is from pupation until eclosion. In preparation for pupation, the larvae of many species construct a protective cocoon of silk or other material, such as its own accumulated faeces. In this stage, the insect's physiology and functional structure, both internal and external, change drastically. Adult holometabolous insects usually have wings and functioning reproductive organs. Mature larvae of different types of insects are commercially available and can be used as protein rich food for animals or humans, such as for the rearing of captive animals in terraria, such as geckos or predatory insects, and for the rearing of larger birds, such as toucans. For example larvae from the pachnoda marginata, also referred to as the pachnoda butana, a beetle from the subfamily Cetoniinae. The adult beetles are 20-30 mm, and the larvae are very small when they hatch, but can grow as long as 60 mm. Other examples may include: - the alphitobius diaperinus, a species of beetle in the family Tenebrionidae, - the zophobas morio, a species of darkling beetle, whose larvae are known by the common name superworm or zophobas, - the mealworm beetle, tenebrio molitor, a species of darkling beetle, the larvae being known as mealworms, - the housefly, Musca domestica, is a fly of the suborder Cyclorrhapha, which larvae are known as maggots, Hermetia illucens, the black soldier fly, is a common and widespread fly - grasshoppers, insects of the order Orthoptera, suborder Caelifera, - crickets family Gryllidae (also known as "true crickets"), are insects related to grasshoppers - crickets known as acheta domesticus. - other insects such as bombyx mori, achroia girsella, gryllodes sigillatus, schistocerca americana gregaria, galleria mellonella, locusta migratoria migratorioides. In a known breeding facilities adult insects including egg-laying mothers are held in containers, in which containers the egg-laying mothers receive food and spawn their eggs. The eggs will hatch, and the baby larvae will mature into mature larvae in the same container, from which the mature larvae are removed to serve as protein rich food. A disadvantage of such a known breeding facility is that the yield of mature larvae is relatively low and unpredictable. For example, egg-laying mothers may eat or damage eggs and baby larvae. Also, it is not possible to simultaneously create optimum conditions for spawning and for hatching, which may e.g. require a different temperature and humidity. The term breeding refers to the natural process of reproduction, involving spawning - the process of releasing and depositing eggs - , and hatching - bringing forth larvae from the egg. It differs from the process of rearing insects, which refers to the process of tending insects from baby-larvae to mature larvae, and possibly also including pupating and emerging into beetles. From WO2014171829 a method and system for rearing insects is known, referring to the process of fending insects from baby-larvae to mature larvae. In a climate area, stacked crates with immature phases of insects are arranged in rows. Periodically, individual crates are conveyed to a feed area provided with an observation station. Based on the observation of a retrieved individual crate it is determined whether supplementary feed stock is to be added, or that the insects are withdrawn from further rearing for harvesting, or discarding from further rearing. The aim of the present invention is to provide an insect breeding facility and method for the industrial scale production of mature larvae from egg-laying mothers. Such industrial scale production requires a continuous and high yield, which is achieved with an insect breeding facility and method according to the present invention. In particular, the present invention relates to an insect breeding facility for the industrial scale production of mature larvae from egg-laying mothers, which mature larvae serve as protein rich food, the insect breeding facility being provided with: - a spawning area in which a number of spawning containers are to be provided or are provided; - a plurality of spawning containers which are adapted to receive or have received adult insects including egg-laying mothers and insect food; - a plurality of spawn structures, wherein at least one spawn structure is adapted to be provided or is provided in each spawning container in the spawning area, in which spawn structures the egg-laying mothers will spawn their eggs during an egg-laying lifetime, such that the spawn structure or part of the spawn structure holds the eggs, wherein said spawn structure or said part thereof is removable from the spawning container; - an insect food delivery system to deliver insect food to the spawning containers; - wherein, in the spawning area: o the egg-laying mothers spawn their eggs in the spawn structures, o insect food is provided to the spawning containers; - a hatch area adapted to receive at least a number of hatching containers, which are adapted to receive the eggs, larvae food and to allow harvesting of mature larvae; - a plurality of hatching containers, in which the eggs are allowed to hatch into baby larvae and subsequently mature into mature larvae during a hatching and tending time, - a larvae food delivery system to deliver larvae food to the hatching containers; wherein every period P2, which period is determined such that the eggs do not yet hatch in the spawning area: - a batch of spawn structures in which the egg-laying mothers have spawned their eggs is removed from the spawning containers and replaced with new spawn structures; - the eggs of the batches of removed spawn structures are transported into a number of empty hatching containers and into the hatch area; such that the eggs of the batch of spawning structures remain separated from the eggs of other batches.
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