Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
" Selective C-H functionalization is a class of reactions that 3. Managing the regioselectivity = making "your" bond react could lead to a paradigm shift in organic synthesis, relying Complex molecules contain numerous C-H bonds that can on selective modifications of ubiquitous C-H bonds of sometimes be differentiated based on steric and electronic organic compounds instead of th standard approach of factors. Various oxidation systems show distinguished conducting transformations on pre-existing functional selectivity in terms of 3°, 2° and 1° C-H bonds. groups." Strategies toward this goal: Davies Nature 2008, 451, 417-424. - use directing groups (functional groups withing the substrate that can coordinate to the metal) Challenges for C-H bond functionalization: - design intramolecular reactions that proceed through a favorable five or six-membered TS 1. Controlling the reactivity -devise supramolecular structures that position the desired Among hydrocarbons, alkanes have long been C-H bond next to the catalyst active site considered inert. Their low reactivity toward reagents is due to their saturation (no low energy empty π orbitals and no high energy filled n orbitals ). 4. Inducing stereoselectivity = functionalize a C-H bond at a prochiral center enantioselectively ΔGC-H (Kcal/mol) pKa Strategies toward this goal (same old...): H 104 ∼ 36 - substrate control (existing chiral centers, chiral auxiliaries 2 - catalyst control CH4 104 48 - functionalize C-H bonds at existing stereocenters with retention or inversion of configuration C2H4 106 50
C2H2 120 24
C6H6 109 43 " One 'Holy Grail' of C-H activation research, therefore, is 2. Achieving chemoselectivity = stopping the reaction at the not simply to find new C-H activation reactions but to obtain correct oxidation state an understanding of them that will allow the development of Strategies toward this goal: reagents capable of selective transformations of C-H bonds - run the reaction at low conversion into more reactive functionalized molecules." - use large excess of substrate vs oxidant Bergman Acc. Chem. Res. 1995, 28, 154-162. - block the overoxidation of the product with functional groups Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Summary of this report The Shilov "electrophilic" process 1. Introduction - Challenges for C-H oxidation 2- 2- PtCl4 - 2. C-H activation by transition metals CH4 + PtCl6 + H2O CH3OH + PtCl4 + 2HCl a. The Shilov process H2O b. Catalytica process 120° C c. Further applications of Pt(II)/Pt(IV) system Shilov Zhurnal Fizicheskoi Khimii 1972, 46, 1353. d. Stoichiometric processes with Pd Shilov Chem. Rev. 1997, 97, 2879-2932. e. Catalytic Pd(II)/Pd(IV) C-H oxidations - first example of a system capable of achieving selective oxidation 3. C-H oxidation with dioxiranes of methane a. Stoichiometric approaches - stoichiometric in Pt(IV) b. Oxidations with DMDO, TFDO in complex systems - shows selectivity for terminal C-H bonds, rather than secondary or c. Fluorinated oxaziridine as stoichiometric oxidant tertiary C-H bonds d. Catalytic oxidation with oxaziridines - intriguing reaction mechanism. Not enough evidence to ascertain 4. C-H oxidation by metal-oxo species that oxidative addition (OA) occurs alone. 5. C-H oxidation by radical mechanisms a. Fenton chemistry Proposed mechanism:
b. Barton and Hofmann-Laffler-Freytag chemistries R-OH 2- Cl Cl R-H 6. Biomimetic approaches to C-H oxidation Pt Cl Cl a. Prophryin systems Cl - Cl Cl b. Gif chemistry R Pt c. Non-heme iron catalysts and mechanism Cl H O Cl d. Applications of non-heme iron catalysts 2
2- 2- H OA Cl Cl Cl R Pt - Pt Cl H Definition: Cl 2 Cl Cl Cl Cl C-H bond activation is the process in which a strong C-H Pt R Cl R + bond is replaced with a weaker, easier to functionalize one. Cl -H
2- Cl Cl Pt + Cl R H 2- [PtCl6] Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Major improvement of the Shilov process For more examples see Shilov Chem. Rev., 1997, 97, 2879; Goldman ACS Symposium Series 885, Activation and (bpym)PtCl2 Functionalization of C-H bonds, 2004. CH4 + 2H2SO4 CH3OSO3H + 2H2O + SO2 100° C Methods for alkane oxidation with transition metals 72% yield (one pass) N N O 81% conversion O CO2H O N N CO H conditions + O + Periana Science 1998, 280, 560. 2 Pt OH Cl Cl for CH4 CH3CO2H see Periana Science 2003, 8.2% 16.2% 2% 301, 814. (bpym)PtCl2 Conditions: K2PtCl4 (0.15 eq), K2PtCl4 (0.3 eq), O2, 90° C, 144h Main features of this process:
a) product is "protected" from overoxidation CO H HO CO H + CO 2 2 2 2 b) the reaction mechanim similar to the one proposed before 17% HO H 1.8% c) SO3 acts as an oxidant OH Et O 3 + The first example of sp C-H oxidative addition CO2H + O 1.3% 2.1% Because they are weak σ-bases and π-acids, alkanes are O poor ligands for metals. They can however form σ-complexes O CO H HO CO2H + + with metals, that are stabilized by π-backbonding from 2 O the metal into C-H σ* orbitals. When such an interaction 6.5% O 23% 3% takes place efficiently, the C-H bond is cleaved and oxidative addition occurs. J. Chem. Soc. Chem. Comm., 1991, 1242 Proposed reaction mechanism: + Pt(II) O + O
hν CO2H Pt Ir H Ir H O O + Pt Me P -H Me P H 3 H 2 3 O II K2PtCl4 5 O2 O 0 from (η -Me5C5)IrH2 Bergman J. Am. Chem. Soc., 1982, 104, 352 -Pt 5 from (η -Me5C5)Ir(CO)2 Graham J. Am. Chem. Soc., 1982, 104, 3723 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
5 mol% K2PtCl4 O O O 1.Na2PdCl4 (1.2 eq) Cl 7 eq. CuCl2 1. Boc2O HO HO O O N NaOAc (1.2 eq), EtOH N Pd OH 2. AcOH Pyr NH + NHBoc 2. Pyr 2 NH3 27% yield 56% yield (crude) 1. Pb(OAc)4 (1eq) 3:1 anti/syn Baldwin Tetrahedron, 1985, 41, 699 AcOH 2. NaBH4 (1 eq) ! No products obtained when Na2S2O8/CuCl2 were used. HO This implies that the reaction doesnt proceed through a N OAc radical mechanism.
O O CO2H quant Cat/Ox NH2 NH CO2H O + O 2 + NH 1. Na PdCl NH 2 4 2 NaOAc prod ratio 2 : 1 : 3 2. Ac2O, Et3N AcO N crude yield 57% HO Cl Pd
Proposed reaction mechanism: N ( 2
CO H E-lupanone oxime 2 Pyr 2- Cl Cl Pt NH2 1. Pb(OAc)4 (1eq) O Cl Cl R AcOH O AcO 2. NaBH4 (1 eq) N H AcO 2- 2 Cl N N Pd R Pt 90% yield Cl NH + Cl O O Pyr 3 OAc R For more applications of this methodology in steroid synthesis: H CuCl H2 2 Studies on Lanostenone E J. Chem. Soc. Perkin Trans. 1, 1988, 1599 Cl N Pt Synthesis of β-Boswellic acid analogues J. Org. Chem., 2000, 65, 6278 Sames Cl O Cl O CuCl J. Am. Chem. Soc., Partial synthesis of Hyptatic Acid-A J. Org. Chem., 2007, 72, 3500 2001, 123, 8149 Pt(IV) Total synthesis of Labatoside E J. Am. Chem. Soc., 2008, 130, 5872 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
MeO MeO 5 mol% Pd(OAc)2 N H N 5 mol% Pd(OAc)2 N 2 eq MeCOOOtBu OAc N 1.1 - 3.2 eq PhI(OAc)2 OAc O O Ac2O, 65 °C, 48 -72h R1 R2 R R 1:1 AcOH:Ac2O or DCM, 1 2 80 - 100 C AcO ° 61% yield AcO MeO OAc AcO OAc N OAc H AcO N OMe OAc N Et N N N Et HO N O O O O O N tBu OAc H 71% 69% 75% 86% 44% 81% AcO 89% OAc AcO Sanford J. Am. Chem. Soc. 2004, 126, 9542 CO2Me N AcO N N tBu tBu O tBu O BuOt O 10 mol% Pd(OAc)2 BuOt O O 50% * * DCM, 50 °C, 40h from alcohol 73%, 24% de 49%, 82% de N H N OAc R 1 eq. IOAc R in SM * Lauroyl peroxide used as oxidant Proposed reaction mechanism: PhI(OAc) + I OAc 2 2 AgOAc + I2 OAc AcO II IV Pd H Pd 2 MeCO tBu 2 Pd(OAc)2 N 3 N OtBu Boc N Boc OMe Et N OAc N OAc Boc Ac O O N OAc Boc O oxidative O 2 MeO N OAc addition R R R1 R2 Et R1 R2 1 2 92% 91% 96% 96% Ac2O Boc Boc Yu Angew. Chem. Int. Ed. N OAc N OAc 2005, 44, 7420 OAc N OtBu AcO IV Pd I 2 Ph OAc N -Pd(OAc) 77% 86% 0% O 2 N OAc O reductive O Yu Org. Lett. 2006, 8, 3387 elimination R1 R2 R1 R2 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Oxidation of sp3 C-H bond with dioxiranes TFDO, 18 min OH O -20 °C, 98% Oxone Oxone O NaHCO O O O O O or DMDO, 17h 3 NaHCO3 F3C F3C rt, 84% DMDO TFDO TFDO, 3 min Murray J. Org. Curci J. Org. Me Me Me Me -20 °C, 98% OH Chem., 1985, 50, Chem., 1988, 53, Me Me 2847 3890 Me Me TFDO, 5 min OH Reaction mechanism: -20 °C, 98% O O- - SO 2- pH 7 - 8 4 R1 O HO SO - or DMDO, 17h + - 3 O SO3 R1 R2 R1 O O slow O rt, 84% R2 R2 OH Useful practical information about dioxiranes: 20 eq TFDO, 3 h - can be isolated and stored (-20 °C) in solution -20 °C, 74% HO - standard concentration for DMDO (0.07 - 0.1M), TFDO (0.8 M ...) OH TFDO 3 DMDO - methods have been described for their in situ generation k ≈ 10 k OH - ketone free solutions can be obtained (in certain cases, the reagent is more potent in a less polar solvent e.g. DCM) TFDO, 1.5 h General oxidation reaction with dioxiranes: -20 °C + O OH O O + S SO + 77% 16% O R1 R2 R1 R2 TFDO, 1 h Chemical properties of dioxiranes: Ph -23 °C, > 95% Ph Et H - electrophilic O-transfer reagents Et OH Me Me - commonly used for epoxidations (alkenes, arenes), oxidations etc. 72% ee 72% ee 3 CH - TFDO is 10 times more reactive than DMDO 3 CH3 1.8 eq TFDO, 40 min OH - dioxiranes generated from chiral ketones can be used in enantio- -20 °C, DCM conv 98% selective transformations (Shi epoxidation) yield 35% - for C-H oxidation 3° > 2° Curci Acc. Chem. Res. 2006, 39, 1 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Dioxiranes as selective oxidants for complex structures
O O 5 eq DMDO 0 C, 2.5h O H H ° 2 eq. DMDO HO O 62% yield
80% yield O AcO AcO AcO AcO Curci J. Org. Chem. 1991, 57, 2182 Curci J. Am. Chem. Soc. 1996, 118, 11089
MeO C 2 MeO2C OH 2 eq TFDO R2 -40 °C, 3h 2 eq DMDO H H AcO AcO H H Br Br 80% yield Br Br AcO AcO H R1 Curci J. Org. Chem. 1991, 57, 5052 R1=OH, R2=H 48% yield R1=OH, R2=OH 36% yield J. Chem. Soc. Perkin Trans. 1, 2001, 2229 O OH 5 eq TFDO O O H -40 °C, 1.5h O H O O 80% yield 2 eq DMDO O O OH rt, 7d O AcO O AcO AcO OH OH AcO 82% yield Fuchs Org. Lett. 2003, 5, 2247 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
OH Intramolecular C-H functionalization with in situ generated O O O dioxiranes O O O Oxone/NaHCO OH O H O 3 OH O R R R O R R1 O R 2 eq DMDO 1 CH3CN/H2O rt 1 OH HO O O OH HO + rt, 48h O O R R2 R2 2 O OH cis trans O OH
C7H15 O CO2Me C7H15 O CO2Me briostatin analogue H O O OH O 70% yield R1 R R1 R Wender Org. Lett. 2005, 7, 79
Proposed reaction mechanism: R2 R2 ‡ γ + H - H α β R δ δ δ• δ• O 1 R O O R O O OH R H + OH OH O R O CF 2 O CO2Me O CO Me 3 R1 R2 R1 R2 2 80% yield 62% yield 78% yield trans/cis 3.4:1 trans/cis 1:10 trans/cis 3.6:1
O H O + R O R O HO R R R 1 OMe 1 2 R2 N 54% yield O N O OH O cis only O CO Me O OH 2 CO2Me H O OR 9% yield R1 45% yield R O + O OH trans/cis 1:1 R1 R2 HO R2 trans only CO2Me OTBS OTBS 43% yield 59% yield OH trans/cis 2.3:1 OH trans/cis 3.1:1 O CO Me O CO2Me 2 Yang J. Am. Chem. Soc., 2003, 125, 158 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Proposed explanation of observed stereochemistries: Oxidation of unactivated sp3 C-H bonds with oxaziridines - easy to prepare from the corresponding α substituent (observed trans/cis 3.4:1) perfluorotrialkylamine (J. Org. Chem., 1993, H R R OH 2 2 R O O 58, 4754) 2 H R2 H C3F7 α R N R H R1 R - powerful oxidants 1 O R O C4F9 F O R O - indefinetely stable at rt R H H OH 1 R1 H O disfavored favored - reacts under neutral or acidic conditions, cis trans in protic or aprotic solvents - selective for tertiary C-H bonds β substituent (observed trans/cis 1:10)
R R2 R OH R2 2 O 2 CO2Me CO Me β H H 2 R H R1 R 1 O R R O R O O O C F H OH CH3 3 7 CH H O R1 H R N 3 favored disfavored 1 C4F9 F cis trans CFCl3, rt AcO AcO 79% yield γ substituent (observed trans/cis 3.6:1) H HO
R H R2 2 OH Resnati J. Org. Chem., 1994, 59, 5511 O H H R R1 H 1 O R R R R O O R2 O R2 4 eq C F O N 3 7 H O H OH R1 H R C4F9 F disfavored favored 1 Br cis trans Br HO CFCl3, 21h, rt HO HO 99% ee 96% ee C8H17 C8H17 C8H17
O 4 eq O C F O N 3 7 C F F HO H 3% O 4% O OH 6% 4 9 Oxone/NaHCO3 H O O CH3CN/H2O HO C H C H CFCl3, 24h, rt O H rt, 41 days C8H17 8 17 8 17 O 99% ee 96% ee F C 3 O O O H 17% O H 10% HO OH 3% Resnati Org. Lett., 1999, 1, 281 Yang J. Org. Chem., 2003, 68, 6321 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
catalytic Substrate scope: R R H 1 C-H oxidation 1 OH N N HO R + O + R OH R2 O R2 R3 R3 HO OBz PivO BzO H O O O O 63% yield 36% yield 43% yield 92% yield S S O N O N active as mCPBA O stoichiometric Du Bois J. Am. Chem. Soc. 2005, 127, 15391 CF 3 CF3 oxidant toward 91% yield 3 adamantane sp C-H oxidation by metal-oxo species
Cl Cl Me 16 mol% MeReO Me Devised catalytic cycle: 3 25 eq. H2O2 OH Me Me O cat tBuOH, 40 °C 98% yield F3C Se O OH HO H O Cl O O SO Me OH 2 OH S N O OH H CF3 F3C O Me
F3C Se 20% yield 20% yield 90% yield 88% yield OH Cl O H2O2 O S S Wearing Tetrahedron Lett. 1995, 36, 6415 N O CF3 Generation of active species: F3C O Me Me Me HO H O 2 2 O O H2O2 O O 20 mol% cat. Re O Re Re O O O O O 1 mol% Ar2Se2 O O 4 eq UHP Hermann Angew. Chem. Int. Ed. Engl. 1993, 32, 1157 DCE, 95h 80% yield Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
O Radical processes for the C-H oxidation of alkanes OH OH O Fenton chemistry O Conditions O - reported as early as 1894 by Fenton (J. Chem. Soc. 1894, 65, OH OH OH 899) - iron (II) salts and H O used for the hydroxylation of alkanes AcO 88% yield 2 2 albeit with poor yields
Conditions: 5 mol% RuCl3• 3H2O, 3 eq. NaBrO3 - selectivity: 3° > 2° > 1° EtOAc/CH3CN/Phosphate buffer = 1:1:2 - Proposed reaction mechanism: Fe(II) + H2O2 Fe(III) + HO + HO• H O R2 R2 2 R R-H + HO• R• + H O OH 2 2 H NaBrO3 O R1 O R1 O Ru O R1 OH R3 Ru R3 R O O 3 R• + O2 R-O-O• R-OH + ketone O + RuO4 + NaBrO2 - + Fuchs J. Org. Chem. 2007, 72, 5820 Fe(III) + H2O2 Fe(II) + HOO• + H
Oxidation of alkanes with strong acids The Hofmann-Loffler-Freitag reaction for C-H activation
O O O UHP, TFA OCOCF3 R R O NHR Br2 O N hν O N Br H R R 80% yield 1 2 R1 R2 R1 R2 Br
OCOCF3 OCOCF3 NR OCOCF3 NHR OH OH hydrolysis O O O O
78% yield 45% yield 67% yield R1 R2 R R R2 1 2 R1 Br Moody Chem. Comm. 2000, 1311 for examples see Baran J. Am. Chem. Soc. 2008, 130, 7247 Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation The Barton reaction for C-H oxidation Proposed reaction mechanism: OAc OAc O O PhI(OAc)2 + tBuOH HO ONO OAc Pyr, NOCl I I tBuOI PhI(OAc)2 OAc
H radical process O O -AcOH corticosterone 1. hν acetate 2. Ac O, pyr 2 I + AcOI OAc O OAc OAc OAc HO O O CH3CO2H N NaNO2 HO Barelunga Angew. Chem. Int. Ed. 2002, 41, 2556.
Biomimetic studies for alkane oxidation O O Various metal porphyrin systems were devised to mimic the Barton J. Am. Chem. Soc. 1960, 82, 2640, 2641 action of Cyt P450 enzymes. Different metals (Fe, Mn, Ru) can accomplish this task together with a diverse range of I I stoichiometric oxidants (PhIO, bleach, oxone, O2 etc). In Conditions OAc general, the transformations (alkane and arene hydroxylation, alkene dehydroxylation) achieved by these systems are poor 71% yield* OAc 92% yield* in yield, chemoselectivity and substrate scope (3° > 2° C-H). I Conditions OAc I For more on the reaction mechanism of Cyt P450 enzymes see Meunier Chem. Rev. 2004, 104, 3947. OAc * excess 47% yield* 65% yield Major players in this field: John T. Groves (Princeton Univ.); Thomas Bruice (UC Santa Barbara); Bernard Meunier (France);
Conditions: 1.1 eq I2, 3.5 eq PhI(OAc)2, 3.5 eq Daniel Mansuy (France). tBuOH, rt. * yield based on I 2 - Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Gif chemistry OH O - developed by Barton at Gif-sur-Ivette and Texas A&M Conditions - stepwise improvement of the system + - the typical GoAgg system consists of Fe(II) salts, picolinic acid - Conditions: 1eq FeL(NCMe)2 cat, 10 eq H2O2, 1000 eq cyclohexane (used as ligand) and oxidant (tBuOOH, H2O2, O2 ) in Pyr/AcOH as solvents L TN (A+K) A:K % incorporation of 18O $ - with adamantane, the selectivity observed shows preferences H 18O H 18O 18O for 2° vs 3° C-H bonds 2 2 2 2 TPA 5:1 27 70 3 - experimental observations refute the possibility of radical 3.2 mechanism BPMEN 6.3 8:1 18 84 0 - Barton argues that the Gif system is biomimetic and the oxidation BQPA 5.8 10:1 7 71 22 V occurs via LFe =O species - 3Me3-TPA 4.5 14:1 30 - Barton Acc. Chem. Res. 1992, 25, 504 6Me3-TPA 1.4 1:1 1 22 77
Non-heme iron catalysts for alkane oxygenation TN = turnonver number (moles of product/moles of iron) $ incorporation in cyclohexanol; Fe cat : H2O2 : H2O : cyclohexane = 1:10:1000:1000 + 2 OH N Various ligands: N NCMe Conditions Fe N N NCMe N N N N N RC = retention of configuration L TN RC (%) general structure of an N N Fe(II) catalyst with a N TPA 3.8 100 tetradentate N4 ligand TPA BPMEN BPMEN 4.6 96 N N N N N N N N BQPA 3.4 89 N 3Me3-TPA 4.5 100 N N N BPQA 6Me3-TPA 1 54 3Me3-TPA 6Me3-TPA Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
Proposed mechanistic pathways for the Fe(TPA) family of For more on mechanistic studies of non-heme Fe catalysts: catalysts: Que J. Am. Chem. Soc. 2001, 123, 6327 18 III O-OH H2O = H2 O Que Chem. Comm. 1999, 1375 (about the BPMEN system) L Fe NCMe Que Chem. Rev. 2004, 104, 939 Pathway a H2O L = TPA OH L = 6Me3TPA (SbF ) Pathway c 6 2 Conditions III O N III O OH -H O V O OH NCMe L Fe 2 V N PivO PivO L Fe L Fe L Fe Fe O H N NCMe O OH O 51% yield H H N > 99:1 dr R-H R-H R-H Pathway b Fe(S,S-PDP) Conditions: 5 mol% Fe(S,S-PDP), III OH 0.2 eq AcOH, 1.2 eq H2O2, OH L Fe R• IV CH3CN, rt (yield based on OH R-OH L Fe R• three iterative additions) 100% RC OH O2 OH MeO OH R-OH Br 3 AcO 3 epimerization OH O 50% R-OH 46% yield 60% yield 52% yield 50% R-OH 100% RC OAc Conclusions: O O O O MeO 1. hindered ligands such as 6Me3TPA favor low-spin Fe(III) - oxo complexes, where the O-O bond is strong. Proton abstraction by these OH 41% yield species is slow and the resulting alkyl radical is poorly quenched by the Fe- 50% yield 70% yield 30% yield of lactone OH species, giving it time to react with O2 from air and to epimerize (from acid) (from ester) (Pathway a) - steric and electronic effects can be used to explain 2. the TPA ligand and other electron rich ligands, favor a high-oxidation state regioselectivity
Fe complex. Isotope labeling studies show that H2O coordination and C-H - the COOH group can be used as a directing group bond cleavage are competitive events Baran Group Meeting Florina Voica 3/21/2009 Alkane Hydroxylation
H H OH
O Conditions O electronic effects O O controlling the O H O H selectivity O O
O O (+) - artemisinin 54% yield
O O O O Conditions no product O HO Me
H OAc H OAc O O Conditions O O AcO AcO H O H OH O O 52% yield (directed hydroxylation) White Science 2007, 318, 783
" The field of alkane activation and functionalization has taken strong hold on chemists' imaginations because it poses hard challenges. The central problem is simply to develop ways to replace selected H substitutents of alkanes by any of a variety of functional groups, X. Progress has been slow - in spite of substantial work on the problem, we are still far from the goal." Crabtree J. Chem. Soc. 2001, 2437-2450.