bioRxiv preprint doi: https://doi.org/10.1101/457895; this version posted October 31, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 Novel 3,6-Dihydroxypicolinic Acid Decarboxylase Mediated Picolinic Acid 2 Catabolism in Alcaligenes faecalis JQ135 3 4 Jiguo Qiu1, Yanting Zhang1, Shigang Yao1, Hao Ren2, Meng Qian3, Qing Hong1, 5 Zhenmei Lu2*, and Jian He1,3 * 6 7 1 Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of 8 Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China 9 2 College of Life Sciences, Zhejiang University, Hangzhou, 310058, China 10 3 Laboratory Centre of Life Science, College of Life Sciences, Nanjing Agricultural University, 11 Nanjing, 210095, China 12 13 Running Title: Novel 3,6-Dihydroxypicolinic Acid Decarboxylase 14 15 Keywords: Alcaligenes faecalis, picolinic acid, 3,6-dihydroxypicolinic acid, 16 degradation, decarboxylase, amidohydrolase_2 17 18 * Address correspondence to Jian He,
[email protected], or Zhenmei Lu, 19
[email protected] 20 21 1 / 25 bioRxiv preprint doi: https://doi.org/10.1101/457895; this version posted October 31, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 22 ABSTRACT 23 Alcaligenes faecalis strain JQ135 utilizes picolinic acid (PA) as sole carbon and 24 nitrogen source for growth. In this study, we screened a 6-hydroxypicolinic acid 25 (6HPA) degradation-deficient mutant through random transposon mutagenesis. The 26 mutant hydroxylated 6HPA into an intermediate, identified as 3,6-dihydroxypicolinic 27 acid (3,6DHPA) with no further degradation.