Research Article 3123 LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK- dependent detachment Andrew Smith*, Madelon Bracke*,‡, Birgit Leitinger§, Joanna C. Porter¶ and Nancy Hogg** Leukocyte Adhesion Laboratory, Cancer Research UK London Research Institute, Lincoln’s Inn Fields Laboratories, Lincoln’s Inn Fields, London WC2A 3PX, UK *These authors contributed equally to this work ‡Present address: Department Pharmacoepidemiology and Pharmacotherapy, Utrecht Institute for Pharmaceutical Sciences, PO Box 80082, 3508 TB Utrecht, The Netherlands §Present address: Sackler Institute for Muscular Skeletal Research, Department of Medicine, University College London, 5 University Street, London WC1E 6JJ, UK ¶Present address: MRC Laboratory for Molecular Cell Biology, University College London, Gordon Street, London WC1E 6BT, UK **Author for correspondence (e-mail:
[email protected]) Accepted 9 April 2003 Journal of Cell Science 116, 3123-3133 © 2003 The Company of Biologists Ltd doi:10.1242/jcs.00606 Summary This study analyzes signaling events initiated through compartmentalized activity of the two kinases is reflected binding of the leukocyte integrin LFA-1 to ICAM-1, which in their localization within the T cell. Myosin light chain leads to T cell attachment, polarization and random kinase is concentrated at the leading edge, overlapping F- migration. These events are critically dependent on actin, whereas ROCK is more widely distributed in the dynamic changes in the acto-myosin cytoskeleton under the trailing edge of the T cell. Thus these two kinases perform regulation of myosin light chain kinase and ROCK (Rho two different functions in the migrating T cell, with myosin kinase).