Animal Cell Technology: Basic & Applied Aspects

Volume 16

For further volumes: http://www.springer.com/series/7552 JAACT 2008 Organizing Committee

Meeting Chairman Masamichi Kamihira (Kyushu University, Japan)

Meeting Vice Chairman Yoshinori Katakura (Kyushu University, Japan)

Meeting Secretaries Akira Ito (Kyushu University, Japan) Hiroyuki Ijima (Kyushu University, Japan) Hiroshi Mizumoto (Kyushu University, Japan) Kiichiro Teruya (Kyushu University, Japan) Yoshinori Kawabe (Kyushu University, Japan)

Organizing Boards: P. M. Alves (Portugal) H. Anazawa (Japan) S. Asahi (Japan) K. Dairiki (Japan) D. Ejima (Japan) A. Enomoto (Japan) Y. Fuke (Japan) Y. Fukui (Japan) S. Hachimura (Japan) S. Hashizume (Japan) C. Hirashima (Japan) T. Hisatsune (Japan) M. Hosobuchi (Japan) H. Hoshi (Japan) S. Iijima (Japan) K. Ikura (Japan) H. Isoda (Japan) T. Kadowaki (Japan) M. Kamei (Japan) Y. Kamei (Japan) S. Kida (Japan) T. Kuwata (Japan) M. Maeda-Yamamoto (Japan) T. Matsuda (Japan) T. Matsumoto (Japan) T. Matsushita (Japan) Y. Miura (Japan) M. Nagao (Japan) H. Nakano (Japan) K. Nishijima (Japan) T. Omasa (Japan) M. Sato (Japan) H. Satsu (Japan) T. Shigehisa (Japan) M. Shimizu (Japan) S. Shirahata (Japan) H. Shinmoto (Japan) M. Takagi (Japan) Y. Taniyama (Japan) M. Tanokura (Japan) M. Totsuka (Japan) M. Tsukahara (Japan) H. Tsukamoto (Japan) H. Tsumura (Japan) K. Yagasaki (Japan) M. Yokota (Japan)

Advisory Boards D. W. Barnes (USA) S. Kaminogawa (Japan) K. Nagai (Japan) R. Sasaki (Japan) G. H. Sato (USA) T. Suzuki (Japan) J. Werenne (Belgium) Masamichi Kamihira · Yoshinori Katakura · Akira Ito Editors

Animal Cell Technology: Basic & Applied Aspects

Proceedings of the 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT), Fukuoka, Japan, November 24–27, 2008

123 Editors Masamichi Kamihira Yoshinori Katakura Kyushu University Kyushu University Fac. Engineering Fac. Agriculture Dept. Chemical Engineering 6-10-1 Hakozaki 744 Motooka Higashi-ku Nishi-ku Fukuoka Fukuoka 812-8581 Japan 819-0395 Japan

Akira Ito Kyushu University Fac. Engineering Dept. Chemical Engineering 744 Motooka Nishi-ku Fukuoka 819-0395 Japan

ISBN 978-90-481-3891-3 e-ISBN 978-90-481-3892-0 DOI 10.1007/978-90-481-3892-0 Springer Dordrecht Heidelberg London New York

Library of Congress Control Number: 2010921921

© Springer Science+Business Media B.V. 2010 No part of this work may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, microfilming, recording or otherwise, without written permission from the Publisher, with the exception of any material supplied specifically for the purpose of being entered and executed on a computer system, for exclusive use by the purchaser of the work.

Printed on acid-free paper

Springer is part of Springer Science+Business Media (www.springer.com) Preface

The 21st Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT) “POWER OF CELLS” was held at the Fukuoka International Congress Center in Fukuoka, Japan from November 24–27, 2008. The meeting focused on recent advancements in the field of animal cell tech- nology from both basic and applied aspects. We invited academic and industrial scientists from all over the world to make this meeting truly successful and scien- tifically fruitful. More than 340 participants from 14 countries joined the meeting and gave presentations (plenary & keynote lectures, 3; Murakami memorial lecture, 1; presentations by symposists, 37; technical seminars, 6; oral presentations, 30; poster presentations, 126). Fields and titles of 9 symposia were 1. “From Molecules and Genes to Therapies: Industrial ACT Perspectives”, 2. “Stem Cells: Culture Technology to Clinical Application – Yasuo Kitagawa Memorial Symposium – ”, 3. “Innovation for Tissue Engineering of Metabolic Organs”, 4. “Cell Engineering – from Omics to Cell Culture Processes”, 5. “Water and Life”, 6. “Nutrigenomics: Lipid Metabolism and Cancer Prevention”, 7. “Recent Progress in Cell Culture Process Development”, 8. “Novel Production System for Biologics”, 9. “ Challenges on Down Stream Process Development for Biologics”. All presentations (oral and poster) were brought together on the following topics. 1. Cell culture engineering 2. Production of biologicals 3. Functional cell lines 4. Glycoengineering 5. Immunologicals, monoclonal antibodies, and vaccines 6. Transplantation, artificial organs, and organ substitutes 7. Gene therapy 8. Transgenic animals 9. Safety and regulation 10. Cell regulatory factors and signal transduction 11. Functional substances in food and natural sources 12. Animal cells for in vitro assay The editors hope this volume of Proceedings will greatly contribute to the development of animal cell technology for innovation of life science.

v vi Preface

The editors express their sincere thanks to all the participants, symposium organizers, chairmen, speakers, members of the organizing committee, and the student staff of this meeting for their valuable contributions. We also deeply thank to Commemorative Organization for The Japan World Exposition ’70, Fukuoka Convention & Visitors Bureau, ThermoFisher Scientific Japan, and Kao Foundation for Arts and Sciences for financial supports.

Japan Masamichi Kamihira Yoshinori Katakura Akira Ito Contents

Purification of Hepatoblasts from Fetal Mouse Livers by Using a Temperature-Reversible Gelation Polymer and Their Application in Regenerative Medicine ...... 1 Koichiro Yori, Toru Koike and Nobuyoshi Shiojiri Computer Aided Rational Design of Biochemical Systems ...... 7 Hiroyuki Kurata DNA Microarray in Nutrigenomics and Cancer Prevention Research: Elucidating Molecular Signatures of Cancer Preventive Phytochemicals ...... 13 Thomas T.Y. Wang DNA Microarray Technology Evaluation of Effect of Dietary Intake of Crude Rice Glycosphingolipids in Mice ...... 19 Jun Shimizu Integrated Pathway Analysis of Genome-Wide Expression Changes Associated with Serum-Free Suspension Adaptation of an Antibody-Producing Chinese Hamster Ovary (CHO) Cell Line .. 27 Yung-Shyeng Tsao, Ankit A. Merchant, Aaron Meyer, and Zhong Liu; Marsha Smith, Diane Levitan, and Eric Gustafson Affordable Mab by Ensuring Low Cog ...... 33 Bjorn Hammarberg and Bo Forsberg Evaluation of Neurotoxicity of Environmental Chemicals Using Neural Stem Cells and Neuroblastoma Cells ...... 41 Masami Ishido Integration of Endothelial Cell-Covered Hepatocyte Spheroids for Construction of Vascularized Liver Tissue ...... 45 Masakazu Inamori, Hiroshi Mizumoto, and Toshihisa Kajiwara Characterization of Cell-Sized Vesicles Induced from Human Lymphoid Cell Lines ...... 51 Makoto Yamanaka, Shigeki Nakamura, Aiko Inoue, Takashi Yasuda, Yuichi Inoue, and Hiroharu Kawahara

vii viii Contents

Dendrimer-Immobilized Culture Surface as a Tool to Promote Aggregate Formation of Anchorage-Dependent Cells ...... 57 Mee-Hae Kim, Masahiro Kino-oka, Jun-ichi Miyazaki, and Masahito Taya Advantage of Alagln as an Additive to Cell Culture Medium ...... 65 Yasufumi Imamoto, Hisaya Tanaka, Ken Takahashi, Yoshinobu Konno, and Toshiyuki Suzawa Supplementation of Phosphatidylcholine Protects the Hybridoma Cells from Apoptosis Induced by Serum Withdrawal .... 73 Beng Ti Tey, Kah Choon Yap, Hideki Yamaji, Abdul Manaf Ali, and Wen Siang Tan Monoclonal Antibody Productivity of Hybridoma Cells Under Active Hypothermic Growth Condition ...... 77 Beng Ti Tey, Sun Li Chong, Michelle Y.T. Ng, Duen Gang Mou, Saw Hoon Lim, and Abdul Manaf Ali Efficient Selection of Cell Clones with Higher Productivity in the Production of Recombinant Human Monoclonal Antibodies .... 83 Ayako Ohshima, Yumiko Takamatsu, Koichi Yamamot, and Hiroyuki Saitoh Continuous Cell Production from Three Dimensional Hematopoietic Microenvironment in Polyurethane Foam ...... 89 Tadasu Jozaki, Kentarou Aoki, Hiroshi Mizumoto, and Toshihisa Kajiwara A New Approach for Drug Discovery and Differentiation Study Using Cutting-Edge 3d Cell Culture System ...... 95 Fujiko Ozawa, Yoshitaka Miyagawa, Masami Hiroyama, Nobutaka Kiyokawa, Akihiro Umezawa, Akito Tanoue, and Satoru Tanaka Dimensional Cell Culture Dish for In Vivo-Like Cell Culture ...... 103 Y. Furutani, T. Tamura, and M. Kodama Characterization of the Erythropoietin (EPO) Protein Production in the Recombinant Human Cultured Cells, in which the Exogenous EPO Gene was Introduced into Hypoxanthine Phosphoribosyl Transferase (HPRT) Gene Locus ...... 109 Toshiaki Banzai, Yukiko Koyama, and Shuji Sonezaki Development of Protein High Expression System by Using Fructose Metabolism ...... 115 Yuriko Tsukamoto, Shigeki Nakamura, Aiko Inoue, Norikazu Nishino, Yuichi Inoue, and Hiroharu Kawahara Contents ix

Controlling Fucosylation Levels of Antibodies with Osmolality during Cell Culture ...... 121 Yoshinobu Konno, Yuki Kobayashi, Ken Takahashi, Eiji Takahashi, Shinji Sakae, Masako Wakitani, Toshiyuki Suzawa, Keiichi Yano, Masamichi Koike, Kaori Wakamatu, and Shinji Hosoi Comparison of Immunoresponses Between Cecal Patch Cells and Peyer’s Patch Cells Stimulated by Bacterial Components ...... 127 Takuma Konno, Akira Hosono, Yasuhiro Hiramatsu, Satoshi Hachimura, Kyoko Takahashi, and Shuichi Kaminogawa Conjugate of β-Lactoglobulin with Carboxymethyl Dextran Increased the Antigen-Specific IgG1 Production, While It Decreased IgE Production Compared with Native β-Lactoglobulin ... 133 Tadashi Yoshida, Yuko Shigihara, Manami Nakamura, and Makoto Hattori Anti-Inflammatory Action of Epigallocatechin-3-Gallate at a Physiological Concentration through the 67-KDA Laminin Receptor in Lipopolysaccharide-Stimulated Raw264.7 Cells ...... 139 Byun Eui Hong, Yoshinori Fujimura, Koji Yamada, and Hirofumi Tachibana Enhancement of Antibody Production using Solution after Antibody Purification: Application of Spent Medium for Antibody Production ...... 145 Akiko Ogawa, Sadaharu Fukui, and Satoshi Terada Production of Human Monoclonal Antibodies Against FC Epsilon Receptor I Alpha by Combining In Vitro Immunization with Phage Display ...... 151 Kosuke Tomimatsu, Shin-ei Matsumoto, Makiko Yamashita, Kiichiro Teruya, Yoshinori Katakura, Shigeru Kabayama, and Sanetaka Shirahata Enhancement of Hepatocyte Function Through Heterotypic Cell-Cell Interactions Using E-Cadherin-Expressing NIH3T3 Cells ... 159 Akira Ito, Takehiko Kiyohara, Yoshinori Kawabe, Hiroyuki Ijima, and Masamichi Kamihira Magnetic Cell-Patterning for Tissue Engineering ...... 165 Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, and Masamichi Kamihira Magnetic Force-Based Tissue Engineering of Skeletal Muscle for Bio-Actuator ...... 171 Yasunori Yamamoto, Masahiro Kato, Akira Ito, Yoshinori Kawabe, Kazunori Shimizu, Eiji Nagamori, and Masamichi Kamihira x Contents

Fabrication of Skeletal Muscle Tissue from C2C12 Myoblast Cell Towards the Use as Bio-Actuator ...... 177 Hideaki Fujita, Kazunori Shimizu, and Eiji Nagamori Retroviral Vectors Pseudotyped with Chimeric Vesicular Stomatitis Virus Glycoprotein for Antibody-Dependent Gene Transduction ...... 185 Yujiro Kameyama, Yoshinori Kawabe, Akira Ito, and Masamichi Kamihira Improvement of CPP-RBD for siRNA Delivery ...... 191 Rina Kuwabara, Tamaki Endoh, Masahiko Sisido, and Takashi Ohtsuki Production of Recombinant Human EPO and EPO/Fc Fusion Proteins by Chinese Hamster Ovary Cells ...... 197 Carlos Alberto Penno, Yoshinori Kawabe, Akira Ito, and Masamichi Kamihira Development of Oviduct-Specific Gene Expression System for Transgenic Avian Bioreactor ...... 203 Yoshinori Kawabe, Kensaku Numata, Masashi Teramori, Akira Ito, and Masamichi Kamihira Production of Therapeutic Proteins Composed of Seven Dominant Human T Cell Epitopes Derived from the Japanese Cedar Pollen Allergens ...... 209 Yoshinori Kawabe, Yoshifumi Hayashida, Kensaku Numata, Akira Hishigae, Akira Ito, and Masamichi Kamihira The Differentiation of C2C12 Cells to Myotube by Paraquat ...... 215 Masaaki Okabe, Koichi Akiyama, Sogo Nishimoto, Takuya Sugahara, and Yoshimi Kakinuma Molecular Cloning and Characterization of a Novel Canine Sulfotransferase ...... 221 Katsuhisa Kurogi, Yoichi Sakakibara, Shin Yasuda, Ming-Cheh Liu, and Masahito Suiko In Vitro Analysis of the Interaction Between Human Intestinal Epithelial Cells and Macrophage-Like Cells ...... 231 Yoko Ishimoto, Hideo Satsu, Tetsunosuke Mochizuki, Mamoru Totsuka, and Makoto Shimizu Role of Ubiquitination Promoted During Restimulation in the Induction of T Cell Anergy ...... 237 Yohei Mizuguchi, Ai Yamamoto, Makoto Hattori, and Tadashi Yoshida Contents xi

Expression Analysis of Senescence-Associated Genes: Their Possible Involvement in Diabetes ...... 243 Miyako Udono, Kaori Mukae, Tsukasa Fujiki, Zhang Ping Bo, Hiroshi Fujii, Sanetaka Shirahata, and Yoshinori Katakura Study of Butyrate Signal Transduction Pathways in Rat Hepatic Stem-Like Cells ...... 247 Toshihiko Saheki, Yusuke Mukai, Ken’ichi Saito, Emi Tajima, Kentaro Katakura, Atsuyoshi Nishina, Mikiko Kishi, Takashi Izumi, Toshihiro Sugiyama, and Itaru Kojima Growth Control of Mammalian Cells via a Human Artificial Chromosome Harboring a Chimeric Receptor ...... 253 Masahiro Kawahara, Toshiaki Inoue, Xianying Ren, Takahiro Sogo, Hidetoshi Yamada, Motonobu Katoh, Hiroshi Ueda, Mitsuo Oshimura, and Teruyuki Nagamune The Impact of Redox State on Regulation of the High-Affinity IgE Receptor Expression ...... 259 Yoshinori Fujimura, Hayato Higo, Satomi Yano, Koji Yamada, and Hirofumi Tachibana The Characteristic of Chimeric Receptors Based on Erythropoietin Receptor ...... 265 Wenhai Liu, Masahiro Kawahara, Hiroshi Ueda, and Teruyuki Nagamune Anti-Cancer and Structure-Activity Relationship of Natural Polyacetylenes ...... 271 Soninkhishig Tsolmon, Yui Kurita, Si Won Hong, Parida Yamada, Hideyuki Shigemori, and Hiroko Isoda Effect of Mongolian Medicinal Plant Stellera Chamaejasme on Chronic Leukemia Cells K562 ...... 277 Soninkhishig Tsolmon, Parida Yamada, and Hiroko Isoda Capsaicin Induced the Upregulation of Transcriptional and Translational Expression of Glycolytic Enzymes Related to Energy Metabolism in Human Intestinal Epithelial Cells ...... 281 Junkyu Han and Hiroko Isoda Effect of Novel Compounds from Thymelaea Hirsuta on Melanogenesis ...... 285 Myra O. Villareal, Yusaku Miyamae, Junkyu Han, Yamada Parida, Hideyuki Shigemori, and Hiroko Isoda Electrolyzed Reduced Water Prolongs Caenorhabditis elegans’ Lifespan ...... 289 Hanxu Yan, Huaize Tian, Takeki Hamasaki, Masumi Abe, Noboru Nakamichi, Kiichiro Teruya, Yoshinori Katakura, Shinkatsu Morisawa, and Sanetaka Shirahata xii Contents

Anti-Cancer Effects of Enzyme-Digested Fucoidan Extract from Seaweed Mozuku ...... 295 Yoshiko Matsuda, Kiichiro Teruya, Sakiko Matsuda, Ayumi Nakano, Takuya Nishimoto, Masashi Ueno, Akitomo Niho, Makiko Yamashita, Hiroshi Eto, Yoshinori Katakura, and Sanetaka Shirahata The Downregulation of Mast Cell Activation Through the Suppression of the High-Affinity IgE Receptor Expression by Green Tea Catechin Egcg ...... 301 Hirofumi Tachibana, Yoshinori Fujimura, Yusuke Hasegawa, Satomi Yano, and Koji Yamada Anti-Diabetes Effects of Hita Tenryou-Sui Water, a Natural Reduced Water ...... 307 Kazuhiro Osada, Yuping Li, Takeki Hamasaki, Masumi Abe, Noboru Nakamichi, Kiichiro Teruya, Yoshitoki Ishii, Ying Wang, Yoshinori Katakura, and Sanetaka Shirahata Suppressive Effect of ERW on Lipid Peroxidation and Plasma Triglyceride Level ...... 315 Masumi Abe, Shunpei Sato, Kazuko Toh, Takeki Hamasaki, Noboru Nakamichi, Kiichiro Teruya, Yoshinori Katakura, Shinkatsu Morisawa, and Sanetaka Shirahata Growth Suppression of HL60 and L6 Cells by Atomic Hydrogen .... 323 Kensuke Nakanishi, Takeki Hamasaki, Takuro Nakamura, Masumi Abe, Kiichiro Teruya, Yoshinori Katakura, Shinkatsu Morisawa, and Sanetaka Shirahata Insulin-Mimetic Activity of Inositol Derivatives Depends on Phosphorylation of PKCζ /λ in L6 Myotubes ...... 327 Nhung Thuy Dang, Masanori Yamaguchi, Tadashi Yoshida, Ken-ichi Yoshida, and Hitoshi Ashida Enhancement of Phagocytic Activity by a Crude Polysaccharide from Tea (Camellia sinensis) Extract ...... 333 Manami Monobe, Kaori Ema, Keiko Azuma, and Mari Maeda-Yamamoto Effects of Lupeol on Melanoma In Vitro and In Vivo: Fundamental and Clinical Trials ...... 339 Keishi Hata, Kikumi Ogihara, Saori Takahashi, Takeshi Tsuka, Saburo Minami, and Yoshiharu Okamoto The Effect of Secoisolariciresinol on 3t3-L1 Adipocytes and the Relationship Between Molecular Structure and the Activity ...... 345 Shiori Tominaga, Takuya Sugahara, Sogo Nishimoto, Manami Yamawaki, Yuki Nakashima, Taro Kishida, Koichi Akiyama, Masafumi Maruyama, and Satoshi Yamauchi Contents xiii

Detection of Anti-Allergic Factors in Strawberry Extracts ...... 353 Kazuhiro Mitsuda, Aiko Inoue, Norikazu Nishino, Yuichi Inoue, and Hiroharu Kawahara Anti-Melanogenic Activity of Yacon Leaves in Mouse Melanoma Cells . 359 Tomoyuki Ishikawa, Toshio Hyakutake, Yasuaki Gondou, Mitsuhiro Sakai, Reiko Kinoshita, Jyunji Tottori, Hiroshi Kunitake, and Ken-Ichi Kusumoto Cytokine Responses of Splenocytes of Female and Male Non-Obese Diabetic Mice Induced by Lactic Acid Bacteria ...... 365 Atsushi Enomoto, Mohd Azraai Bin Jamal, Hiromi Mitsui, Hiromi Kimoto-Nira, Chise Suzuki, and Koko Mizumachi Development of a High-Throughput Evaluation System for Antiviral Activities of Food Factors By Using Bioinformatics ...... 371 Nozomu Eto, Kiyoko Nagahama, Shohei Miyamoto, Osayoshi Yamaguchi, Sayumi Miyauchi, Takako Iwata, Asuka Uchida, and Kunihito Yamamori The Effects of Pesticides on Immune Cells ...... 377 Sogo Nishimoto, Kota Kanda, Masaaki Okabe, Koichi Akiyama, Yoshimi Kakinuma, and Takuya Sugahara Co-Cultured Hepatocyte-Spheroid Chip Constructed Using the Microfabrication Technique ...... 383 Yusuke Sakai and Kohji Nakazawa Micropatterned Hepatocyte Culture Using Microstencil Technique ... 391 Yukako Shinmura, Ami Higuchi, Yusuke Sakai, and Kohji Nakazawa Structural Features of Totally Designed Lipidic Materials for Small Interfering RNA Delivery System ...... 397 Ken-Ichi Kusumoto Thin-Film Assembly of Totally Designed Lipidic Materials as Gene Carrier in Mouse Embryonic Neural Stem Cells: Thin-Film Assembly of Lipidic Material as Potential Gene Carrier ... 403 Ken-Ichi Kusumoto, Takahiro Nagata, and Itaru Hamachi Transfection System Using a Cocktail of Totally Designed Lipidic Materials in Chinese Hamster Ovary Cells: Lipidic Cocktail as Potential Gene Carrier for CHO Cells ...... 409 Ken-Ichi Kusumoto, Takahiro Nagata, Eiichi Kanazawal, Takanori Sakai, Yoshihiko Yoshikawa, Takahiro Ogata, Naomi Nakatani, Tomoyuki Ishikawa, Shintaro Koga, Akio Shirasu, Shinji Morishita, Takayuki Emura, and Itaru Hamachi xiv Contents

Size Dependent Heat Generation of Magnetic Nanoparticles Under AC Magnetic Field for Cancer Therapy ...... 415 Jun Motoyama, Toshiyuki Hakata, Ryuji Kato, Noriyuki Yamashita, Tomio Morino, Takeshi Kobayashi, and Hiroyuki Honda Functional Evaluation of Anticancer Drugs in Human Leukemia Cells Based on Metabolic Profiling Technique ...... 423 Daisuke Miura, Yoshinori Fujimura, Hirofumi Tachibana, and Hiroyuki Wariishi Extracts from the Root of Platycodon grandiflorum Modulate Induction of Inflammatory Changes in Coculture of Adipocytes and Macrophages ...... 429 Ju-Hye Lee, Koji Yamada, and Hirofumi Tachibana Position Specific Incorporation of Nonnatural Amino Acids into Proteins In Vivo ...... 435 Satoshi Neki, Takashi Ohtsuki, and Masahiko Sisido Novel Protease Inhibitor Inhibiting Proliferation of Influenza Virus: Purification and cDNA Cloning of a Novel Protease Inhibitor Secreted by MDCK Cells ...... 441 Kiyoto Nishiyama, Keishin Sugawara, Kenji Soejima, Shin-ichi Abe, and Hiroshi Mizokami Contributors

Masumi Abe Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan, [email protected] Shin-ichi Abe Graduate School of Science and Technology, Kumamoto University, Kurokamimachi 2-39-1, Kumamoto 860-8555, Japan Hirokazu Akiyama Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan Koichi Akiyama Integrated Center for Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan; Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan Abdul Manaf Ali Department of Cell and Molecular Biology, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor Darul Ehsan, Malaysia Kentarou Aoki Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka, Japan Hitoshi Ashida Graduate School of Agriculture Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan Keiko Azuma National Institute of Vegetable and Tea Science, NARO, Shizuoka, Japan Toshiaki Banzai Biotechnology Group, Research Institute, TOTO LTD., Kitakyushu-city, Fukuoka, 802-8601, Japan Zhang Ping Bo Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Sun Li Chong Biotechnology Program, Malaysia University of Science and Technology, Kelana Jaya, Selangor 47301, Malaysia Nhung Thuy Dang Graduate School of Agriculture Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan

xv xvi Contributors

Kaori Ema National Institute of Vegetable and Tea Science, NARO, Shizuoka, Japan Takayuki Emura ASTEC Corporation, Shime-machi, Fukuoka 811-2207, Japan Tamaki Endoh Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan Atsushi Enomoto Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Gunma 376-8515, Japan, [email protected] Nozomu Eto University of Miyazaki, Miyazaki 889-2192, Japan Hiroshi Eto Daiichi Sangyo Co. Ltd., Japan Bo Forsberg CMC Biologics A/S, Copenhagen, DK-2860, Denmark Hiroshi Fujii Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Tsukasa Fujiki Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Yoshinori Fujimura Innovation Center for Medical Redox Navigation, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan, [email protected] Yoshinori Fujimura Innovation Center for Medical Redox Navigation, Kyushu University, Fukuoka 812-8582, Japan, [email protected] Hideaki Fujita Toyota Central R&D Labs. Inc., Aichi 480-1192, Japan Sadaharu Fukui Department of Chemistry and Biochemistry, Suzuka National College of Technology, Suzuka, Mie 510-0294, Japan Y. Furutani Vessel Inc., Japan Yasuaki Gondou Kurume City Office, Kurume, Fukuoka 830-8520, Japan Eric Gustafson Functional Genomics, Discovery Technologies, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA Satoshi Hachimura Research Center for Food Safety, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo 113-8657, Japan Toshiyuki Hakata Toda Kogyo Corp., Hiroshima, 739-0652 Japan Itaru Hamachi Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Katsura, Kyoto 615-8510, Japan Takeki Hamasaki Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan Bjorn Hammarberg ABD Life Sciences Ltd, Central, Hong Kong Junkyu Han Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Contributors xvii

Byun Eui Hong Department of Bioscience and Biotechnology, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Si Won Hong Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Yusuke Hasegawa Kyushu University, Fukuoka 812-8581, Japan Keishi Hata Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Araya-machi, Akita 010-1623, Japan, [email protected] Makoto Hattori Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan Yoshifumi Hayashida Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan Hayato Higo Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Ami Higuchi Department of Life and Environment Engineering, University of Kitakyushu, Kitakyushu, Fukuoka 808-0135, Japan Yasuhiro Hiramatsu Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan Masami Hiroyama Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan Akira Hishigae Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan Hiroyuki Honda Graduate School of Engineering, Nagoya University, Nagoya, 464-8603 Japan Shinji Hosoi Kyowa Hakko Kirin Co., LTD., Chiyoda-ku, Tokyo, 100-8185, Japan Akira Hosono Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan, [email protected] Toshio Hyakutake Department of Biological Chemistry, Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan Hiroyuki Ijima Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka 819-0395, Japan Yasufumi Imamoto Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan xviii Contributors

Masakazu Inamori Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka, 819-0395, Japan Aiko Inoue The Cell Engineering Center, Kitakyushu National College of Technology, Kitakyushu-shi, Fukuoka, 802-0985, Japan; The Cell Engineering Center, Kitakyushu National College of Technology, Kokuraminami-ku, Kitakyushu 802-0985, Japan Toshiaki Inoue Department of Human Genome Science, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan Yuichi Inoue The Cell Engineering Center, Kitakyushu National College of Technology, Kitakyushu-shi, Fukuoka, 802-0985, Japan; The Cell Engineering Center, Kitakyushu National College of Technology, Kokuraminami-ku, Kitakyushu 802-0985, Japan Masami Ishido Environmental Risk Research Programme, National Institute for Environmental Studies, Tsukuba, Ibaraki 305-8506, Japan, [email protected] Yoshitoki Ishii Hita Tenryousui Co. Ltd., Hita 877-0074, Japan Tomoyuki Ishikawa Department of Biological Chemistry, Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan Yoko Ishimoto Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Hiroko Isoda Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan; Alliance for Research on North Africa, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Akira Ito Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819–0395, Japan [email protected] Takako Iwata Miyazaki prefectural industrial support foundation, Miyazaki 880-0303, Japan Takashi Izumi Department of Molecular Biochemistry, Gunma University, Graduate School of Medicine, Maebashi, Japan Mohd Azraai Bin Jamal Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Gunma 376-8515, Japan Tadasu Jozaki Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka, Japan Shigeru Kabayama Nihon Trim Co. Ltd., Oyodonaka, Kita-ku, Osaka 531-0076, Japan Contributors xix

Toshihisa Kajiwara Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka, 819-0395, Japan, [email protected] Yoshimi Kakinuma Integrated Center for Sciences (INCS), Ehime University, Tarumi, Matsuyama 790-8566, Japan; Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan Yujiro Kameyama Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan Masamichi Kamihira Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Graduate School of Systems Life Science, Kyushu University, Fukuoka 819-0395, Japan Shuichi Kaminogawa Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan Eiichi Kanazawa Department of Biological Chemistry, Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan Kota Kanda Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan Kentaro Katakura Department of Biological and Chemical Engineering, Gunma University, Kiryu, Japan Yoshinori Katakura Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan, [email protected]; Graduate School of System Life Science, Kyushu University, Fukuoka 812-0053, Japan, [email protected]; Department of Genetic Resources Technology, Kyushu University, Fukuoka 812-8581, Japan, [email protected] Masahiro Kato Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka 819-0395, Japan Ryuji Kato Graduate School of Engineering, Nagoya University, Nagoya, 464-8603 Japan Motonobu Katoh Department of Human Genome Science, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan Yoshinori Kawabe Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan, [email protected] Hiroharu Kawahara The Cell Engineering Center, Kitakyushu National College of Technology, Kitakyushu-shi, Fukuoka, 802-0985, Japan xx Contributors

Masahiro Kawahara Center for NanoBio Integration (CNBI), The University of Tokyo, Tokyo 113-8656, Japan; Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan, [email protected] Mee-Hae Kim Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 565-0871, Japan Hiromi Kimoto-Nira Functional Biomolecules Research Team, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan Masahiro Kino-oka Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 565-0871, Japan Reiko Kinoshita Kurume Research Park, Kurume, Fukuoka 839-0864, Japan Mikiko Kishi Department of Molecular Biochemistry, Gunma University, Graduate School of Medicine, Maebashi, Japan Taro Kishida Ehime University, Matsuyama, Ehime 790-8566, Japan Takehiko Kiyohara Graduate School of Systems Life Sciences, Kyushu University, Nishi-ku, Fukuoka 819-0395, Japan Nobutaka Kiyokawa Department of Development Biology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan Yuki Kobayashi Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Takeshi Kobayashi School of Bioscience and Biotechnology, Chubu University, Kasugai, 487-8501 Japan M. Kodama Vessel Inc., Japan Shintaro Koga Department of Biological Chemistry, Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan Toru Koike Department of Biology, Shizuoka University, Suruga-ku, Shizuoka-City, Shizuoka 422-8529, Japan Masamichi Koike BioWa, Inc., Princeton, NJ, 08540, USA Itaru Kojima Department of Molecular Medicine, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan Yoshinobu Konno Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan Takuma Konno Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan Contributors xxi

Yukiko Koyama Biotechnology Group, Research Institute, TOTO LTD., Kitakyushu-city, Fukuoka, 802-8601, Japan Hiroshi Kunitake Kurume City Office, Kurume, Fukuoka 830-8520, Japan Hiroyuki Kurata Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka, 820-8502, Japan Yui Kurita Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Katsuhisa Kurogi Department of Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki 889-2192 Japan, [email protected] Ken-Ichi Kusumoto Department of Biological Chemistry, Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center, Kurume, Fukuoka 839-0861, Japan Rina Kuwabara Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan Ju-Hye Lee Kyushu University, Higashi-ku, Fukuoka, Japan Diane Levitan Functional Genomics, Discovery Technologies, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA Yuping Li Department of Genetic Resources Technology, Kyushu University, Fukuoka 812-8581, Japan Saw Hoon Lim Biotechnology Program, Malaysia University of Science and Technology, Kelana Jaya, Selangor 47301, Malaysia Zhong Liu Protein Expression Technologies, GBSPD, Schering-Plough Research Institute, Union, NJ 07083, USA Wenhai Liu Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo 113-8656, Japan Ming-Cheh Liu Department of Pharmacology, College of Pharmacy, The University of Toledo, Toledo, OH 43606 USA Mari Maeda-Yamamoto National Institute of Vegetable and Tea Science, NARO, Shizuoka, Japan Masafumi Maruyama Ehime University, Matsuyama, Ehime 790-8566, Japan Yoshiko Matsuda Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan, [email protected] Sakiko Matsuda Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan xxii Contributors

Shin-ei Matsumoto Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Ankit A. Merchant Protein Expression Technologies, GBSPD, Schering-Plough Research Institute, Union, NJ 07083, USA Aaron Meyer Bioengineering Department, University of California, Los Angeles, CA 90024, USA Saburo Minami Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Koyama-Minami, Tottori, Japan Kazuhiro Mitsuda Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Wakamatsu-ku, Kitakyushu-shi, Fukuoka 808-0196, Japan, mitti_ [email protected] Hiromi Mitsui Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Gunma 376-8515, Japan Daisuke Miura Innovation Center for Medical Redox Navigation, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan Yoshitaka Miyagawa Department of Development Biology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan Yusaku Miyamae Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Shohei Miyamoto University of Miyazaki, Miyazaki 889-2192, Japan Sayumi Miyauchi University of Miyazaki, Miyazaki 889-2192, Japan Jun-ichi Miyazaki Division of Stem Cell Regulation Research, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan Hiroshi Mizokami The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Kumamoto University, Kikuchi, Kumamoto 869-1298, Japan Yohei Mizuguchi Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan, [email protected] Koko Mizumachi Functional Biomolecules Research Team, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan Hiroshi Mizumoto Department of Chemical Engineering, Kyushu University, Nishi-ku, Fukuoka 819-0395, Japan Tetsunosuke Mochizuki Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Manami Monobe National Institute of Vegetable and Tea Science, NARO, Shizuoka, Japan, [email protected] Contributors xxiii

Tomio Morino Nanotherapy Co., Ltd, Nagoya, 451-0044 Japan Shinkatsu Morisawa Nihon Trim co. LTD, Osaka, Osaka 531-0076, Japan Shinji Morishita ASTEC Corporation, Shime-machi, Fukuoka 811-2207, Japan Jun Motoyama Graduate School of Engineering, Nagoya University, Nagoya, 464-8603 Japan Duen Gang Mou Biotechnology Program, Malaysia University of Science and Technology, Kelana Jaya, Selangor 47301, Malaysia Kaori Mukae Graduate School of System Life Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Yusuke Mukai Department of Biological and Chemical Engineering, Gunma University, Kiryu, Japan Kiyoko Nagahama Miyazaki prefectural industrial support foundation, Miyazaki 880-0303, Japan Eiji Nagamori Toyota Central R&D Labs. Inc., Aichi 480-1192, Japan, [email protected] Teruyuki Nagamune Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan; Department of Bioengineering, The University of Tokyo, Tokyo 113-8656, Japan Takahiro Nagata Dojindo Laboratories, Mashiki-machi, Kumamoto 861-2202, Japan Noboru Nakamichi Nihon Trim Co. Ltd., Osaka 531-0076, Japan; Functional Water Cell Analysis Center Co. Ltd., Fukuoka 812-0013, Japan Shigeki Nakamura The Cell Engineering Center, Kitakyushu National College of Technology, Kitakyushu-shi, Fukuoka, 802-0985, Japan Manami Nakamura Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan Takuro Nakamura Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan Shigeki Nakamura The Cell Engineering Center, Kitakyushu National College of Technology, Kitakyushu-shi, Fukuoka, 802-0985, Japan Kensuke Nakanishi Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan Ayumi Nakano Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan Yuki Nakashima Ehime University, Matsuyama, Ehime 790-8566, Japan xxiv Contributors

Naomi Nakatani Research and Development Laboratory, Nipro Corporation, Kusatsu-shi, Shiga 525-0055, Japan Kohji Nakazawa Department of Life and Environment Engineering, The University of Kitakyushu, Kitakyushu, Fukuoka 808-0135, Japan, [email protected] Satoshi Neki Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan Michelle Y.T. Ng Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Akitomo Niho Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan Sogo Nishimoto Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577, Japan, [email protected] Takuya Nishimoto Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan Atsuyoshi Nishina Gunma Industrial Technology Center, Maebashi, Japan Norikazu Nishino Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu-shi, Fukuoka, 808-0196, Japan Kiyoto Nishiyama The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Kikuchi, Kumamoto 869-1298, Japan Kensaku Numata Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan Takahiro Ogata ASTEC Corporation, Shime-machi, Fukuoka 811-2207, Japan Akiko Ogawa Department of Chemistry and Biochemistry, Suzuka National College of Technology, Suzuka, Mie 510-0294, Japan Kikumi Ogihara Department of Pathology, Azabu University, Sagamihara, Kanagawa 229-8501, Japan Ayako Ohshima Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan Takashi Ohtsuki Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan; Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan Masaaki Okabe Center for Marine Environmental Studies (CMES), Ehime University, Matsuyama, Ehime 790-8577, Japan, [email protected] Yoshiharu Okamoto Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Koyama-Minami, Tottori, Japan Contributors xxv

Kazuhiro Osada Department of Genetic Resources Technology, Kyushu University, Fukuoka 812-8581, Japan Mitsuo Oshimura Department of Human Genome Science, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan Fujiko Ozawa SCIVAX Corporation, Kawasaki, Japan Yamada Parida Alliance for Research on North Africa, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Carlos Alberto Penno Graduate School of Systems Life Science, Kyushu University, Fukuoka 819–0395, Japan Xianying Ren Department of Human Genome Science, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan Toshihiko Saheki Department of Biological and Chemical Engineering, Gunma University, Kiryu, Japan, [email protected] Ken’ichi Saito Department of Biological and Chemical Engineering, Gunma University, Kiryu, Japan Hiroyuki Saitoh Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan Shinji Sakae Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Mitsuhiro Sakai Kurume City Office, Kurume, Fukuoka 830-8520, Japan Yusuke Sakai Department of Life and Environment Engineering, The University of Kitakyushu, Fukuoka 808-0135, Japan Takanori Sakai ASTEC Corporation, Shime-machi, Fukuoka 811-2207, Japan Yoichi Sakakibara Department of Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki 889-2192, Japan Shunpei Sato Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan Hideo Satsu Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Hideyuki Shigemori Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Yuko Shigihara Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan Jun Shimizu Department of Clinical Dietetics and Human Nutrition, Josai University, Sakado-shi, Saitama 350-0295, Japan xxvi Contributors

Kazunori Shimizu Toyota Central R&D Labs. Inc., Aichi 480-1192, Japan Makoto Shimizu Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan, [email protected] Yukako Shinmura Department of Life and Environment Engineering, University of Kitakyushu, Kitakyushu, Fukuoka 808-0135, Japan Nobuyoshi Shiojiri Department of Biology, Shizuoka University, Suruga-ku, Shizuoka-City, Shizuoka 422-8529, Japan, [email protected] Sanetaka Shirahata Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan, [email protected] Akio Shirasu Research and Development Laboratory, Nipro Corporation, Kusatsu-shi, Shiga 525-0055, Japan Masahiko Sisido Graduate School of Natural Science and Technology, Okayama University, Okayama, Japan; Department of Bioscience and Biotechnology, Okayama University, Okayama, Japan Marsha Smith Functional Genomics, Discovery Technologies, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA Kenji Soejima The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Kumamoto University, Kikuchi, Kumamoto 869-1298, Japan Takahiro Sogo Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo 113-8656, Japan Shuji Sonezaki Biotechnology Group, Research Institute, TOTO LTD., Kitakyushu-city, Fukuoka, 802-8601, Japan, [email protected] Takuya Sugahara Ehime University, Tarumi, Matsuyama 790-8566, Japan Keishin Sugawara The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, Kumamoto University, Kikuchi, Kumamoto 869-1298, Japan Toshihiro Sugiyama Department of Biochemistry, Akita University, School of Medicine, Akita, Japan Masahito Suiko Department of Biochemistry and Applied Biosciences, University of Miyazaki, Miyazaki 889-2192, Japan Toshiyuki Suzawa Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Chise Suzuki Functional Biomolecules Research Team, National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, Japan Hirofumi Tachibana Bio-Architecture Center, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan, [email protected] Contributors xxvii

Emi Tajima Department of Biological and Chemical Engineering, Gunma University, Kiryu, Japan Ken Takahashi Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Eiji Takahashi Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Kyoko Takahashi Department of Food Science and Technology, College of Bioresource Sciences, Nihon University, Fujisawa-shi, Kanagawa 252-8510, Japan Saori Takahashi Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Araya-machi, Akita 010-1623, Japan Yumiko Takamatsu Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan T. Tamura Vessel Inc., Japan Wen Siang Tan Department of Microbiology, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Hisaya Tanaka Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan Satoru Tanaka SCIVAX Corporation, Kawasaki, Japan Akito Tanoue Department of Pharmacology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan Masahito Taya Division of Chemical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 565-0871, Japan Satoshi Terada Department of Applied Chemistry and Biotechnology, University of Fukui, Fukui 910-8507, Japan Masashi Teramori Department of Chemical Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan Kiichiro Teruya Graduate School of Systems Life Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan; Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan; Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan Beng Ti Tey Institute of Bioscience, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia; Department of Chemical and Environmental Engineering, Faculty of Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia, [email protected] xxviii Contributors

Huaize Tian Department of Genetic Resources Technology, Kyushu University, Fukuoka 812-8581, Japan Kazuko Toh Department of Genetic Resources Technology, Kyushu University, Fukuoka, Fukuoka 812-8581, Japan Kosuke Tomimatsu Graduate School of Systems Life Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Shiori Tominaga Ehime University, Matsuyama, Ehime 790-8566, Japan, [email protected] Mamoru Totsuka Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan Jyunji Tottori Kurume City Office, Kurume, Fukuoka 830-8520, Japan Yung-Shyeng Tsao Protein Expression Technologies, BSPD, Merck Research Laboratories, Union, NJ 07083, USA, [email protected] Soninkhishig Tsolmon Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Takeshi Tsuka Department of Veterinary Clinical Medicine, School of Veterinary Medicine, Tottori University, Koyama-Minami, Tottori, Japan Yuriko Tsukamoto Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu-shi, Fukuoka, 808-0196, Japan Asuka Uchida Miyazaki prefectural industrial support foundation, Miyazaki 880-0303, Japan Miyako Udono Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Hiroshi Ueda Department of Chemistry and Biotechnology, The University of Tokyo, Tokyo 113-8656, Japan Masashi Ueno Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan Akihiro Umezawa Department of Reproductive Biology, National Research Institute for Child Health and Development, Setagaya-ku, Tokyo, Japan Myra O. Villareal Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Kaori Wakamatu Graduate School of Engineering, Gunma University, Tenjin-cho, Kiryu-shi 376-8515, Japan Masako Wakitani Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Contributors xxix

Thomas T.Y. Wang Diet, Genomics and Immunology Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, USDA, Beltsville, MD, USA Ying Wang Department of Geriatrics and Cardiovascular Sciences, Central Hospital of Changchun, Changchun, Jilin 130051, China Hiroyuki Wariishi Bio-Architecture Center, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Koji Yamada Department of Bioscience and Biotechnology, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Hidetoshi Yamada Department of Human Genome Science, Graduate School of Medical Science, Tottori University, Yonago, Tottori 683-8503, Japan Parida Yamada Alliance for Research on North Africa, Universitiy of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan Koji Yamada Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Masanori Yamaguchi Hokko Chemical Industry Co., Ltd, Atsugi, Kanagawa 243-0023, Japan Osayoshi Yamaguchi University of Miyazaki, Miyazaki 889-2192, Japan Hideki Yamaji Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, Nada, Kobe 657-8501, Japan, [email protected] Kunihito Yamamori University of Miyazaki, Miyazaki 889-2192, Japan Koichi Yamamot Bio Process Research and Development Laboratories, Production Division, Kyowa Hakko Kirin Co., Ltd., Takasaki-shi, Gunma 370-0013, Japan Yasunori Yamamoto Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan, [email protected] Ai Yamamoto Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan Makoto Yamanaka Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu-shi, Fukuoka, 808-0196, Japan Makiko Yamashita Department of Genetic Resources Technology, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan; Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan Noriyuki Yamashita Nanotherapy Co., Ltd, Nagoya, 451-0044 Japan Satoshi Yamauchi Ehime University, Matsuyama, Ehime 790-8566, Japan xxx Contributors

Manami Yamawaki Ehime University, Matsuyama, Ehime 790-8566, Japan Hanxu Yan Graduate School of System Life Science, Kyushu University, Fukuoka 812-0053, Japan Keiichi Yano Bioprocess Research and Development Laboratories, Kyowa Hakko Kirin Co., LTD., Takasaki-shi, Gunma 370-0013, Japan Satomi Yano Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan Kah- Choon Yap Department of Chemical and Environmental Engineering, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia Takashi Yasuda Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu-shi, Fukuoka, 808-0196, Japan Shin Yasuda Department of Bioscience, School of Agriculture, Tokai University, Aso, Kumamoto 869-1404 Japan Koichiro Yori Department of Biology, Shizuoka University, Suruga-ku, Shizuooka-City, Shizuoka 422-8529, Japan Tadashi Yoshida Fujicco Co., Ltd, Kobe, Hyogo 650-8558, Japan; Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan, [email protected] Ken-ichi Yoshida Graduate School of Agriculture Science, Kobe University, Nada-ku, Kobe, Hyogo 657-8501, Japan Yoshihiko Yoshikawa Research and Development Laboratory, Nipro Corporation, Kusatsu-shi, Shiga 525-0055, Japan