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Characterization of Two Undescribed Mucoralean Species with Specific
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 26 March 2018 doi:10.20944/preprints201803.0204.v1 1 Article 2 Characterization of Two Undescribed Mucoralean 3 Species with Specific Habitats in Korea 4 Seo Hee Lee, Thuong T. T. Nguyen and Hyang Burm Lee* 5 Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, 6 Chonnam National University, Gwangju 61186, Korea; [email protected] (S.H.L.); 7 [email protected] (T.T.T.N.) 8 * Correspondence: [email protected]; Tel.: +82-(0)62-530-2136 9 10 Abstract: The order Mucorales, the largest in number of species within the Mucoromycotina, 11 comprises typically fast-growing saprotrophic fungi. During a study of the fungal diversity of 12 undiscovered taxa in Korea, two mucoralean strains, CNUFC-GWD3-9 and CNUFC-EGF1-4, were 13 isolated from specific habitats including freshwater and fecal samples, respectively, in Korea. The 14 strains were analyzed both for morphology and phylogeny based on the internal transcribed 15 spacer (ITS) and large subunit (LSU) of 28S ribosomal DNA regions. On the basis of their 16 morphological characteristics and sequence analyses, isolates CNUFC-GWD3-9 and CNUFC- 17 EGF1-4 were confirmed to be Gilbertella persicaria and Pilobolus crystallinus, respectively.To the 18 best of our knowledge, there are no published literature records of these two genera in Korea. 19 Keywords: Gilbertella persicaria; Pilobolus crystallinus; mucoralean fungi; phylogeny; morphology; 20 undiscovered taxa 21 22 1. Introduction 23 Previously, taxa of the former phylum Zygomycota were distributed among the phylum 24 Glomeromycota and four subphyla incertae sedis, including Mucoromycotina, Kickxellomycotina, 25 Zoopagomycotina, and Entomophthoromycotina [1]. -
Taxonomy and Epidemiology of <I>Mucor Irregularis</I> , Agent Of
UvA-DARE (Digital Academic Repository) Taxonomy and epidemiology Mucor irregularis, agent of chronic cutaneous mucormycosis Lu, X.L.; Najafzadeh, M.J.; Dolatabadi, S.; Ran, Y.P.; Gerrits van den Ende, A.H.G.; Shen, Y.N.; Li, C.Y.; Xi, L.Y.; Hao, F.; Zhang, Q.Q.; Li, R.Y.; Hu, Z.M.; Lu, G.; Wang, J.J.; Drogari- Apiranthitou, M.; Klaassen, C.; Meis, J.F.; Hagen, F.; Liu, W.D.; de Hoog, G.S. DOI 10.3767/003158513X665539 Publication date 2013 Document Version Final published version Published in Persoonia - Molecular Phylogeny and Evolution of Fungi Link to publication Citation for published version (APA): Lu, X. L., Najafzadeh, M. J., Dolatabadi, S., Ran, Y. P., Gerrits van den Ende, A. H. G., Shen, Y. N., Li, C. Y., Xi, L. Y., Hao, F., Zhang, Q. Q., Li, R. Y., Hu, Z. M., Lu, G., Wang, J. J., Drogari-Apiranthitou, M., Klaassen, C., Meis, J. F., Hagen, F., Liu, W. D., & de Hoog, G. S. (2013). Taxonomy and epidemiology Mucor irregularis, agent of chronic cutaneous mucormycosis. Persoonia - Molecular Phylogeny and Evolution of Fungi, 30, 48-56. https://doi.org/10.3767/003158513X665539 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. -
A Newly Isolated Rhizopus Microsporus Var. Chinensis Capable of Secreting Amyloytic Enzymes with Raw-Starch-Digesting Activity
J. Microbiol. Biotechnol. (2010), 20(2), 383–390 doi: 10.4014/jmb.0907.07025 First published online 24 December 2009 A Newly Isolated Rhizopus microsporus var. chinensis Capable of Secreting Amyloytic Enzymes with Raw-Starch-Digesting Activity Li, Yu-Na1,2, Gui-Yang Shi1,2, Wu Wang1,2*, and Zheng-Xiang Wang1,2* 1The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China 2Research Center of Bio-resource and Bio-energy, School of Bioengineering, Jiangnan University, Wuxi 214122, China Received: July 19, 2009 / Revised: November 15, 2009 / Accepted: November 17, 2009 A newly isolated active producer of raw-starch-digesting are available, but researchers continue to search for new amyloytic enzymes, Rhizopus microsporus var. chinensis amyloytic enzymes that could create new applications. CICIM-CU F0088, was screened and identified by The traditional course of enzymatic starch saccharification morphological characteristics and molecular phylogenetic is a high-energy-consumption procedure, which significantly analyses. This fungus was isolated from the soil of Chinese enhances the cost of starch-related products. Therefore, to glue pudding mill, and produced high levels of amylolytic reduce the cost of starch processing, the importance of activity under solid-state fermentation with supplementation enzymatic saccharification of raw starch without cooking of starch and wheat bran. Results of thin-layer chromatography has become well recognized recently [24, 34]. This has showed there are two kinds of amyloytic enzymes formed generated a worldwide interest in the discovery of several by this strain, including one α-amylase and two glucoamylases. raw-starch-digesting amyloytic enzymes that do not require It was found in the electron microscope experiments that gelatinization and can directly hydrolyze the raw starch in the two glucoamylases can digest raw corn starch and a single step [26, 34]. -
Distribution of Methionine Sulfoxide Reductases in Fungi and Conservation of the Free- 2 Methionine-R-Sulfoxide Reductase in Multicellular Eukaryotes
bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Distribution of methionine sulfoxide reductases in fungi and conservation of the free- 2 methionine-R-sulfoxide reductase in multicellular eukaryotes 3 4 Hayat Hage1, Marie-Noëlle Rosso1, Lionel Tarrago1,* 5 6 From: 1Biodiversité et Biotechnologie Fongiques, UMR1163, INRAE, Aix Marseille Université, 7 Marseille, France. 8 *Correspondence: Lionel Tarrago ([email protected]) 9 10 Running title: Methionine sulfoxide reductases in fungi 11 12 Keywords: fungi, genome, horizontal gene transfer, methionine sulfoxide, methionine sulfoxide 13 reductase, protein oxidation, thiol oxidoreductase. 14 15 Highlights: 16 • Free and protein-bound methionine can be oxidized into methionine sulfoxide (MetO). 17 • Methionine sulfoxide reductases (Msr) reduce MetO in most organisms. 18 • Sequence characterization and phylogenomics revealed strong conservation of Msr in fungi. 19 • fRMsr is widely conserved in unicellular and multicellular fungi. 20 • Some msr genes were acquired from bacteria via horizontal gene transfers. 21 1 bioRxiv preprint doi: https://doi.org/10.1101/2021.02.26.433065; this version posted February 27, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. -
Over Production of Milk Clotting Enzyme from Rhizomucor Miehei Through Adjustment of Growth Under Solid State Fermentation Conditions
Australian Journal of Basic and Applied Sciences, 6(8): 579-589, 2012 ISSN 1991-8178 Over Production of Milk Clotting Enzyme from Rhizomucor miehei Through Adjustment of Growth Under Solid State Fermentation Conditions 1Mohamed S. FODA, 1Maysa E. MOHARAM, 2Amal RAMADAN and 1Magda A. El-BENDARY 1 Microbial Chemistry Department, National Research Centre, Dokki, Giza, Egypt. 2 Biochemistry Department, National Research Centre, Dokki, Giza, Egypt. Abstract: The present study introduces a novel biotechnology for cost effective and economically feasible production of natural fungal rennin in Egypt to fulfill the local requirements of cheese industry instead of the calf rennet that is highly expensive and imported from abroad. In this study, Rhizomucor miehei NRRL 2034 exhibited the highest enzyme productivity under solid state fermentation. Among twelve industrial by-products used as nutrient substrates for Rhizomucor miehei growth under solid state fermentation, wheat bran yielded the highest milk clotting activity (33350 SU/g fermented culture). The optimum conditions for milk clotting enzyme production were moisture content of 50%, incubation temperature 40°C, incubation period 3 days and inoculums size of 1.8 × 108 CFU/g wheat bran. Addition of whey powder as a nitrogen source at 2% increased the enzyme productivity about 42% compared to the control medium. Wheat bran particle size more than 300 µm was the most suitable size for the highest production of the enzyme. The maximum milk clotting activity was achieved by using mineral solution as a moistening agent for wheat bran. Large scale production of the enzyme in tray showed the maximum activity when 125 grams wheat bran were distributed in an aluminum foil trays ( 20×25 ×5 cm 3) under the optimum cultural conditions. -
Molecular Phylogenetic and Scanning Electron Microscopical Analyses
Acta Biologica Hungarica 59 (3), pp. 365–383 (2008) DOI: 10.1556/ABiol.59.2008.3.10 MOLECULAR PHYLOGENETIC AND SCANNING ELECTRON MICROSCOPICAL ANALYSES PLACES THE CHOANEPHORACEAE AND THE GILBERTELLACEAE IN A MONOPHYLETIC GROUP WITHIN THE MUCORALES (ZYGOMYCETES, FUNGI) KERSTIN VOIGT1* and L. OLSSON2 1 Institut für Mikrobiologie, Pilz-Referenz-Zentrum, Friedrich-Schiller-Universität Jena, Neugasse 24, D-07743 Jena, Germany 2 Institut für Spezielle Zoologie und Evolutionsbiologie, Friedrich-Schiller-Universität Jena, Erbertstr. 1, D-07743 Jena, Germany (Received: May 4, 2007; accepted: June 11, 2007) A multi-gene genealogy based on maximum parsimony and distance analyses of the exonic genes for actin (act) and translation elongation factor 1 alpha (tef ), the nuclear genes for the small (18S) and large (28S) subunit ribosomal RNA (comprising 807, 1092, 1863, 389 characters, respectively) of all 50 gen- era of the Mucorales (Zygomycetes) suggests that the Choanephoraceae is a monophyletic group. The monotypic Gilbertellaceae appears in close phylogenetic relatedness to the Choanephoraceae. The mono- phyly of the Choanephoraceae has moderate to strong support (bootstrap proportions 67% and 96% in distance and maximum parsimony analyses, respectively), whereas the monophyly of the Choanephoraceae-Gilbertellaceae clade is supported by high bootstrap values (100% and 98%). This suggests that the two families can be joined into one family, which leads to the elimination of the Gilbertellaceae as a separate family. In order to test this hypothesis single-locus neighbor-joining analy- ses were performed on nuclear genes of the 18S, 5.8S, 28S and internal transcribed spacer (ITS) 1 ribo- somal RNA and the translation elongation factor 1 alpha (tef ) and beta tubulin (βtub) nucleotide sequences. -
<I>Mucorales</I>
Persoonia 30, 2013: 57–76 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X666259 The family structure of the Mucorales: a synoptic revision based on comprehensive multigene-genealogies K. Hoffmann1,2, J. Pawłowska3, G. Walther1,2,4, M. Wrzosek3, G.S. de Hoog4, G.L. Benny5*, P.M. Kirk6*, K. Voigt1,2* Key words Abstract The Mucorales (Mucoromycotina) are one of the most ancient groups of fungi comprising ubiquitous, mostly saprotrophic organisms. The first comprehensive molecular studies 11 yr ago revealed the traditional Mucorales classification scheme, mainly based on morphology, as highly artificial. Since then only single clades have been families investigated in detail but a robust classification of the higher levels based on DNA data has not been published phylogeny yet. Therefore we provide a classification based on a phylogenetic analysis of four molecular markers including the large and the small subunit of the ribosomal DNA, the partial actin gene and the partial gene for the translation elongation factor 1-alpha. The dataset comprises 201 isolates in 103 species and represents about one half of the currently accepted species in this order. Previous family concepts are reviewed and the family structure inferred from the multilocus phylogeny is introduced and discussed. Main differences between the current classification and preceding concepts affects the existing families Lichtheimiaceae and Cunninghamellaceae, as well as the genera Backusella and Lentamyces which recently obtained the status of families along with the Rhizopodaceae comprising Rhizopus, Sporodiniella and Syzygites. Compensatory base change analyses in the Lichtheimiaceae confirmed the lower level classification of Lichtheimia and Rhizomucor while genera such as Circinella or Syncephalastrum completely lacked compensatory base changes. -
DNA Barcoding in <I>Mucorales</I>: an Inventory of Biodiversity
UvA-DARE (Digital Academic Repository) DNA barcoding in Mucorales: an inventory of biodiversity Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, W.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; de Hoog, G.S. DOI 10.3767/003158513X665070 Publication date 2013 Document Version Final published version Published in Persoonia - Molecular Phylogeny and Evolution of Fungi Link to publication Citation for published version (APA): Walther, G., Pawłowska, J., Alastruey-Izquierdo, A., Wrzosek, W., Rodriguez-Tudela, J. L., Dolatabadi, S., Chakrabarti, A., & de Hoog, G. S. (2013). DNA barcoding in Mucorales: an inventory of biodiversity. Persoonia - Molecular Phylogeny and Evolution of Fungi, 30, 11-47. https://doi.org/10.3767/003158513X665070 General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulations If you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: https://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. UvA-DARE is a service provided by the library of the University of Amsterdam (https://dare.uva.nl) Download date:29 Sep 2021 Persoonia 30, 2013: 11–47 www.ingentaconnect.com/content/nhn/pimj RESEARCH ARTICLE http://dx.doi.org/10.3767/003158513X665070 DNA barcoding in Mucorales: an inventory of biodiversity G. -
Molecular Identification of Fungi
Molecular Identification of Fungi Youssuf Gherbawy l Kerstin Voigt Editors Molecular Identification of Fungi Editors Prof. Dr. Youssuf Gherbawy Dr. Kerstin Voigt South Valley University University of Jena Faculty of Science School of Biology and Pharmacy Department of Botany Institute of Microbiology 83523 Qena, Egypt Neugasse 25 [email protected] 07743 Jena, Germany [email protected] ISBN 978-3-642-05041-1 e-ISBN 978-3-642-05042-8 DOI 10.1007/978-3-642-05042-8 Springer Heidelberg Dordrecht London New York Library of Congress Control Number: 2009938949 # Springer-Verlag Berlin Heidelberg 2010 This work is subject to copyright. All rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilm or in any other way, and storage in data banks. Duplication of this publication or parts thereof is permitted only under the provisions of the German Copyright Law of September 9, 1965, in its current version, and permission for use must always be obtained from Springer. Violations are liable to prosecution under the German Copyright Law. The use of general descriptive names, registered names, trademarks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. Cover design: WMXDesign GmbH, Heidelberg, Germany, kindly supported by ‘leopardy.com’ Printed on acid-free paper Springer is part of Springer Science+Business Media (www.springer.com) Dedicated to Prof. Lajos Ferenczy (1930–2004) microbiologist, mycologist and member of the Hungarian Academy of Sciences, one of the most outstanding Hungarian biologists of the twentieth century Preface Fungi comprise a vast variety of microorganisms and are numerically among the most abundant eukaryotes on Earth’s biosphere. -
Mucormycosis of the Central Nervous System
Journal of Fungi Review Mucormycosis of the Central Nervous System 1 1,2, , 3, , Amanda Chikley , Ronen Ben-Ami * y and Dimitrios P Kontoyiannis * y 1 Infectious Diseases Unit, Tel Aviv Sourasky Medical Center, Tel Aviv 64239, Israel 2 Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv 64239, Israel 3 Department of Infectious Diseases, The University of Texas, M.D. Anderson Cancer Center, Houston, TX 77030, USA * Correspondence: [email protected] (R.B.-A.); [email protected] (D.P.K.) These authors contribute equally to this paper. y Received: 6 June 2019; Accepted: 7 July 2019; Published: 8 July 2019 Abstract: Mucormycosis involves the central nervous system by direct extension from infected paranasal sinuses or hematogenous dissemination from the lungs. Incidence rates of this rare disease seem to be rising, with a shift from the rhino-orbital-cerebral syndrome typical of patients with diabetes mellitus and ketoacidosis, to disseminated disease in patients with hematological malignancies. We present our current understanding of the pathobiology, clinical features, and diagnostic and treatment strategies of cerebral mucormycosis. Despite advances in imaging and the availability of novel drugs, cerebral mucormycosis continues to be associated with high rates of death and disability. Emerging molecular diagnostics, advances in experimental systems and the establishment of large patient registries are key components of ongoing efforts to provide a timely diagnosis and effective treatment to patients with cerebral mucormycosis. Keywords: central nervous system; mucormycosis; Mucorales; zygomycosis 1. Introduction Mucormycosis is the second most frequent invasive mold disease after aspergillosis [1–3], with rising incidence reported in some countries [4–7]. -
Thesis FINAL PRINT
Preface This study was conducted at the Department of Chemistry, Biotechnology and Food Science (IKBM), Norwegian University of Life Sciences (UMB) during November 2009 to November 2010. My supervisors were Professor Dr Arne Tronsmo and PhD student Md. Hafizur Rahman, Department of IKBM, UMB. I express my sincere appreciation and gratitude to Professor Arne Tronsmo and Dr. Linda Gordon Hjeljord for their dynamic guidance throughout the period of the study, constant encouragement, constructive criticism and valuable suggestion during preparation of the thesis. I also want to thank Md. Hafizur Rahman for his guidance during my research work. Moreover I express my sincere gratitude to Grethe Kobro and Else Maria Aasen and all other staffs and workers at IKBM, UMB for their helpful co-operation to complete the research work in the laboratory. Last but not the least; I feel the heartiest indebtedness to Sabine and my family members for their patient inspirations, sacrifices and never ending encouragement. Ås, January 2011 Latifur Rahman Shovan i Abstract This thesis has been focused on methods to control diseases caused by Botrytis cinerea. B. cinerea causes grey mould disease of strawberry and chickpea, as well as many other plants. The fungal isolates used were isolated from chickpea leaf (Gazipur, Bangladesh) or obtained from the Norwegian culture collections of Bioforsk (Ås) and IKBM (UMB). Both morphological and molecular characterization helped to identify the fungal isolates as Botrytis cinerea (B. cinerea 101 and B. cinerea-BD), Trichoderma atroviride, T. asperellum Alternaria brassicicola, and Mucor piriformis. The identity of one fungal isolate, which was obtained from the culture collection of Bioforsk under the name Microdochium majus, could not be confirmed in this study. -
Thermophilic Fungi: Taxonomy and Biogeography
Journal of Agricultural Technology Thermophilic Fungi: Taxonomy and Biogeography Raj Kumar Salar1* and K.R. Aneja2 1Department of Biotechnology, Chaudhary Devi Lal University, Sirsa – 125 055, India 2Department of Microbiology, Kurukshetra University, Kurukshetra – 136 119, India Salar, R. K. and Aneja, K.R. (2007) Thermophilic Fungi: Taxonomy and Biogeography. Journal of Agricultural Technology 3(1): 77-107. A critical reappraisal of taxonomic status of known thermophilic fungi indicating their natural occurrence and methods of isolation and culture was undertaken. Altogether forty-two species of thermophilic fungi viz., five belonging to Zygomycetes, twenty-three to Ascomycetes and fourteen to Deuteromycetes (Anamorphic Fungi) are described. The taxa delt with are those most commonly cited in the literature of fundamental and applied work. Latest legal valid names for all the taxa have been used. A key for the identification of thermophilic fungi is given. Data on geographical distribution and habitat for each isolate is also provided. The specimens deposited at IMI bear IMI number/s. The document is a sound footing for future work of indentification and nomenclatural interests. To solve residual problems related to nomenclatural status, further taxonomic work is however needed. Key Words: Biodiversity, ecology, identification key, taxonomic description, status, thermophile Introduction Thermophilic fungi are a small assemblage in eukaryota that have a unique mechanism of growing at elevated temperature extending up to 60 to 62°C. During the last four decades many species of thermophilic fungi sporulating at 45oC have been reported. The species included in this account are only those which are thermophilic in the sense of Cooney and Emerson (1964).