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Etude Physico-Chimique Et Valorisation De Composés Bioactifs De Parinari Macrophylla Sabine (Chrysobalanaceae) Mamadou Balde

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Etude Physico-Chimique Et Valorisation De Composés Bioactifs De Parinari Macrophylla Sabine (Chrysobalanaceae) Mamadou Balde Etude physico-chimique et valorisation de composés bioactifs de Parinari macrophylla Sabine (Chrysobalanaceae) Mamadou Balde To cite this version: Mamadou Balde. Etude physico-chimique et valorisation de composés bioactifs de Parinari macro- phylla Sabine (Chrysobalanaceae). Chimie théorique et/ou physique. Université de Strasbourg; Uni- versité Cheikh Anta Diop (Dakar), 2018. Français. NNT : 2018STRAF013. tel-01980092 HAL Id: tel-01980092 https://tel.archives-ouvertes.fr/tel-01980092 Submitted on 14 Jan 2019 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. UNIVERSITE DE STRASBOURG Ecole Doctorale des Sciences Chimiques – ED 222 UNIVERSITE CHEIKH ANTA DIOP DE DAKAR Ecole Doctorale des Sciences de la Vie, de la Santé et de l’Environnement THÈSE en Convention de Cotutelle Internationale présentée par : Mamadou BALDE Soutenue le : 31 Août 2018 pour obtenir le grade de : Docteur de l’université de Strasbourg Discipline/ Spécialité : Chimie/Chimie Physique Etude physico-chimique et valorisation de composés bioactifs de Parinari macrophylla Sabine (Chrysobalanaceae) THÈSE dirigée par : M. Eric MARCHIONI Professeur, Université de Strasbourg (France) M. Alassane WELE Professeur, Université Cheikh Anta DIOP de Dakar (Sénégal) RAPPORTEURS : M. Abdoulaye DIENG Professeur, Université de Thiès (Sénégal) M. Gora MBAYE Professeur, Université Cheikh Anta DIOP de Dakar (Sénégal) AUTRE MEMBRE DU JURY : Mme. Diane JULIEN-DAVID MCF, Université de Strasbourg (France) Je dédie ce travail : A mon père et ma mère A mes Frères et Sœurs A mon épouse et mes enfants A mes Cousins et Cousines A mes Oncles et Tantes A mes Ami(e)s REMERCIEMENTS Cette thèse de doctorat s’inscrit dans le cadre d’une cotutelle internationale entre l’Université Cheikh Anta Diop de Dakar et l’Université de Strasbourg. Les travaux ont été réalisée en collaboration entre les Laboratoires de : Chimie Physique, Chimie Organique, Chimie Minérale et Chimie Thérapeutique de la Faculté de Médecine, de Pharmacie et d’Odontologie de l’Université Cheikh Anta Diop de Dakar Et Chimie Analytique des Molécules Bioactives et Pharmacognosie (CAMBAP, UMR 7178, DSA, IPHC) de la Faculté de Pharmacie de l’Université de Strasbourg Cette thèse a été réalisée sous la direction des Professeurs Alassane WELE et d’Éric MARCHIONI. Je remercie toutes ces personnes qui m’ont soutenu et les structures et institutions qui ont contribué pour la réalisation de ces travaux. Je remercie la Direction de la Recherche de France, le Service de Coopération et d’Action Culturelle (SCAC) de l’Ambassade de la France au Sénégal et le Ministère de l’Enseignement Supérieur et de la Recherche Scientifique au Sénégal qui ont bien voulu financer ce travail de thèse. J’adresse mes vifs remerciements au Pr Alassane WELE. J’ai connu en lui une personne sociale, sympathique, rigoureuse et clairvoyante. Pr WELE est tellement exigeant envers lui- même qu’il est toujours en perpétuelle recherche du travail parfait. Tu nous as toujours soutenu et encouragé. Nous savons que c’est loin d’être fini mais nous te remercions pour ta confiance et tes conseils aguerris du début jusqu’à la fin de ces travaux de thèse. Je remercie le Pr Eric MARCHIONI pour sa confiance et de m’avoir accueilli au sein de son laboratoire pour m’avoir soutenu tout au long de ces trois années de thèse. Tu as toujours été là pour moi et m’as écouté avec autant d’attention quel que soit la question ou le sujet malgré votre calendrier très chargé et je n’oublie pas toutes ces tracasseries avec l’administration de l’école doctorale, depuis le dépôt des dossiers jusqu’aux formalités administratives pour la soutenance. Tu m’as soutenu jusqu’au bout et tu n’avais même pas hésiter à m’affecter une partie de ton bureau pendant l’un des moments les plus difficiles de la thèse qu’est la rédaction, pour avoir de meilleures conditions de travail. Je te témoigne toute ma reconnaissance et ma profonde gratitude. Je remercie infiniment Madame Diane JULIEN-DAVID pour sa grande disponibilité, son encadrement, ses conseils et encouragements sans faille. Et MERCI également pour ces longues heures passées ensemble pendant les derniers jours de rédaction de mon manuscrit, qui duraient dès fois jusque tard le soir. Je remercie les membres du jury, pour nous avoir fait l’honneur et d’accepter de juger ce travail : Pr Abdoulaye DIENG, Pr Gora MBAYE, Pr Bara NDIAYE. Je remercie les Professeurs Mbacké SEMBENE et Mounibé DIARRA, respectivement Directeurs de l’ED-SEV et de la Formation Doctorale Science du Médicament. Mes remerciements vont aussi à l’endroit de mes maîtres et collègues de labo de l’Université Cheikh Anta DIOP de Dakar les Professeurs Djibril FALL et Matar SECK, les Docteurs Idrissa NDOYE, Adama DIEDHIOU et Yoro TINE. Je remercie particulièrement Mme Rokhaya Sylla GUEYE pour tout son soutien, ses encouragements, et toutes ces heures de cours assurées à mon absence, je t’en serai toujours reconnaissant. A Mme FAYE Christelle Sadio qui en plus de son rôle de personne focale pour la bonne marche du service, s’est toujours investie jusqu’à la dernière énergie pour toutes les démarches administratives. Elle est aidée dans ses missions par son binôme de tous les jours et un pilier incontournable M. Ibrahima KEITA, une personne qui de par sa simplicité, son humanisme arrivera sans doute au soumet de ses ambitions. En vous deux, Je vous remercie infiniment !!! Je vous témoigne tout mon amitié ! Je remercie également Mme DAFFE, M. GUEYE, pour leurs encouragements, et leur soutien. Je remercie les Professeurs Yérim Mbagnick DIOP, Serigne Omar SARR et Dr Rokahaya GUEYE mais aussi Mme Rose DIENE (communément appelée Tata Rose par les étudiants), Sokhna, Claire et tout le personnel du Laboratoire de Chimie Analytique et Bromatologie du Département de Pharmacie de la FMPO pour leurs soutiens et encouragements. Mention spéciale au Pr Amadou DIOP qui m’a toujours impressionné par sa modestie, sa courtoisie, sa pertinence et toutes ses valeurs humaines. Tu m’as toujours soutenu, encouragé et orienté et si j’en suis là aujourd’hui tu en fait parti, alors je te témoigne toute ma gratitude. Je voudrais remercier une personne discrète, humble, sympathique tant de qualités qui n’ont rien à voir avec ses qualités de chercheur avéré dans son domaine, je veux nommer le Pr Alioune Dior FALL, tu m’as toujours soutenu et encouragé et m’as aidé à faire la sélection de mes plantes d’étude. Je donne une mention spéciale à mes amis et collègues Mbaye SENE et Mady Sy pour ces bons moments passés ensemble en Alsace. Merci pour tout ! Je remercie tous les maîtres et collègues du Département de Pharmacie pour leurs soutien et encouragements. Je remercie également tous mes ami(e)s et mes camarades de la promo sortante de Pharmacie 2009 sans citer de noms de peur d’en oublier. Ce travail est également le vôtre. Je voudrais associer mes sincères remerciements à mes meilleur(e)s ami(e)s Fatoumata BAH, Jeannot BASSENE et Moussa BOIRO. Merci également au personnel du Décanat de la FMPO et à tout le personnel de l’UCAD pour leur soutien et accompagnement. Du côté de CAMBAP, Un grand merci à Saïd pour sa rigueur scientifique et son grand apport pour le côté technique, ses conseils et ses encouragements. Ton regard critique et tes suggestions m’ont toujours été très utiles pour la suite de mes travaux. Je ne remercierai jamais assez Ludivine, pour toutes ces longues séances de travail et surtout son sens de la méthode, sa pertinence et sa rigueur scientifique (Coucou à Célyan !!!). Je remercie vivement Martine, Sonia, Minjie et Christophe pour leurs remarques pendant les réunions de labo et qui ont toujours été présents pour m’aider à avancer au travers de discussions toujours pertinentes. Je tiens à remercier également le personnel de la Société TWISTAROMA, Céline et Damien pour les séances de travail et leurs encouragements et Nathalie pour sa sympathie. Je remercie aussi Aurélie pour l’accueil et l’accompagnement pour la réalisation des tests de caractérisation phytochimiques et Mélanie pour ses corrections de la partie RMN et ses conseils. Merci à Myriam, pour tout son soutien côté administratif et mais aussi dans la préparation des commandes ! Merci à tous les « anciens » et « actuels » doctorants de l’Equipe de Chimie Analytique des Molécules Bioactives et Pharmacognosie : Michel, Génildo, Yasmine, Randa, Kamar, Ikram, Zélie, Yé, pour la belle ambiance au labo. A Zélie et Ye, je vous encourage et vous souhaite bonne chance pour la suite !!! Un grand merci à mon très cher ami, Clément Huguet pour ta sympathie et tes encouragements. Je n’oublierai jamais les bons moments passés ensemble et surtout suite à la victoire de l’équipe de France pour obtenir le ticket de la finale au jardin des deux rives conduisant à l’obtention de son 2ème ETOILE (**) dans l’histoire de la coupe du monde :). Un grand merci aussi à l’ensemble du personnel du Centre Européen d’Etude du Diabète (CEED) notamment, Pr Pinget, Séverine Sigrist, Stéphanie, Karim, Elisa, Harzo, Elodie, William, Allan, Claude merci pour votre soutien et la belle ambiance ! Mention spéciale à Cynthia qui m’a initié à la culture cellulaire et qui a assuré mes dernières manips. Tu as été là du début à la fin des tests bio.
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