MD. MORSALINE BILLAH Professor Biotechnology and Genetic Engineering Discipline Khulna University, Khulna-9208 Bangladesh Tel: 88-041-2831546 (Off.), Mobile: 880-1712-008393 E-mail: [email protected] Date of Birth: January 01, 1978
Profile An enthusiastic and proactive biotechnologist with extensive experience in teaching and research at undergraduate and graduate level students and proficient in theoretical and experimental research activities in allied fields of interest with good expertise on bioprospecting of plant and microbial resources, bionanotechnology, curriculum design and development having strong interest in educating students and sharing knowledge to broader audience in research and academia
EDUCATIONAL QUALIFICATIONS
PhD in Biochemistry and Microbiology (2004 to 2008) Institute of Membrane and Systems Biology, University of Leeds, UK Thesis: Development of impedimetric immunosensors for biomarkers of cardiac pathology
Bachelor of Science in Biotechnology (4 years) (1995 to 2000) Results: CGPA 3.95 in a scale of 4.0 (Ranked first) Khulna University, Khulna, Bangladesh
PROFESSIONAL EXPERIENCE
Visiting Researcher (September 2016August 2017) School of Biological Sciences, Roger Land Building, Kings Buildings, The University of Edinburgh, Edinburgh EH3 8FF, United Kingdom
Visiting Professor (June 2013June 2014) School of Engineering, EME4242 3333 University Way, The University of British Columbia, Kelowna, British Columbia, Canada V1V 1V7
Professor (May 2013 till date) Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh Major Courses on offer: Bionanotechnology, Vaccine Technology, Genomics and Proteomics, Microbial Biotechnology, Animal Cell Culture, Molecular Biology, Immunology and Biostatistics
Associate Professor (May 2009May 2013) Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh Courses offered: Bionanotechnology, Vaccine Technology, Genomics and Proteomics,
1 | Page Microbial Biotechnology, Animal Cell Culture, Immunology and Biostatistics
Postdoctoral Research Scientist (December 2011May 2012) Kulliyyah of Science, International Islamic University Malaysia, Kuantan, Malaysia
Assistant Professor (May 2005May 2009) Biotechnology and Genetic Engineering Discipline, Khulna University, Khulna, Bangladesh Courses offered: Immunology, Bioinformatics, Instrumentation Analysis, Molecular Biology and Microbial Genetics.
Research Fellow (January 2008June 2008) Biosensor and Biocatalysis Research Group, University of Leeds, UK
Postgraduate Research Student (2004-2008) Faculty of Biological Sciences, University of Leeds, UK Lecturer (2001 to 2004) Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh
Undergraduate Demonstrator (2004-2007) Faculty of Biological Sciences, University of Leeds, UK
RESEARCH EXPERIENCE
Principal Investigator (2012-2014) Funded by International Development Research Centre (IDRC), Canada on Detection of Pathogens in Drinking Water to Prevent Waterborne Disease Transmission in Developing Countries in collaboration with University of British Columbia, Canada
Project Team Leader (2011-2013) Funded by INSPIRE (International Strategic Partnerships in Research & Education ‘Strategic Partnership), British council on “A research partnership to explore the indigenous and unique medicinal plants of the Sundarbans, the world’s largest mangrove forest, in search for new medicine” in collaboration with University of Wolverhampton, UK
Postgraduate Research Student (2004-2008) University of Leeds, UK
LABORATORY AND EXPERIMENTAL SKILLS Electrochemistry Techniques A range of electrochemistry techniques including electrochemical impedance spectroscopy, cyclic voltammetry, and amperometric techniques
Characterization Techniques Atomic Force Microscopy (both in air and liquid) , Langmuir Blodgett technique , Quartz Crystal Microbalance (QCM) for deposition kinetics, Surface Plasmon Resonance (SPR) for affinity measurements, Scanning Electron Microscopy
2 | Page Antibody handling Techniques Antibody purification, affinity column preparation, preparation of reduced antibody fragments etc
Health and Safety Competent in risk assessment and COSHH assessments for both processes and chemicals Attended courses on biological, chemical, radiation safety Licensed to handle with radioactive chemicals
Molecular Biology, Immunology and Microbiology Techniques Polymerase Chain Reaction, Isolation and Quantification of Plasmid and genomic DNA, Gel Electrophoresis (Agarose and SDSPAGE), Southern and Northern Blotting, Immunoprecipitaion, DNA Footprinting, Cell Culture, Cell Counting, Enzyme Linked Immunosorbent Assay (ELISA), Immunohistology and immunohistochemistry, Culturing and aseptic techniques, Enumerating bacteria and identification
ADMINISTRATIVE EXPERIENCE Curriculum Development for undergraduate and postgraduate level. Worked as expert member in curricula development for both these levels for different public university including Khulna University, Jessore Science and Technology University, Mawalana Bhashani Science and Technology University, Jahangirnagar University, Islamic University etc. Course Coordinators for undergraduate and postgraduate levels in different academic sessions Student Counsellor
PRESENTATIONS
Posters and Abstracts Mixed self-assembled monolayers (SAMs) for immunosensor construction, 6-10 November 05, 2nd International Workshop on Surface Modification for Chemical and Biochemical Sensing SMCBS 2005, Poland Development of immunosensors and other affinity sensors based on mixed self-assembled monolayers, 5-8 October 2006, 5th Aegean Analytical Chemistry Days, Thessaloniki, Greece Directed immobilisation of reduced antibody fragments onto a self-assembled 4 - aminothiophenol monolayer for myoglobin immunosensor, 1-4 April 2007, XIXth International Symposium on Bioelectrochemistry and Bioenergetics, Toulouse, France Development of impedimetric immunosensoers for biomarkers of cardiac pathology, 7-12 September 2008, the 59th Annual Meeting of the International Society of Electrochemistry (Electrochemistry down to the molecular level: interfacial science for life and technology), Seville, Spain Association of Vitamin D Receptor Gene G>T and T>C Polymorphisms in the Pathogenesis of Impaired Glucose Tolerance in a Subjects of Bangladeshi Origin, 14 July
3 | Page 2012, Green Chemistry for Sustainable Development, Jessore Science and Technology University, Jessore, Bangladesh Oriented Immobilization of Antibodies towards Functional Nanobiosensors for Point-of care Monitoring, 21-23 September 2012, International Workshop on Nanotechnology, Dhaka, Bangladesh Nanostructure-mediated Drug Delivery and Its Technological Implications, 21-23 September 2012, International Workshop on Nanotechnology, Dhaka, Bangladesh Food Nanotechnology-A Promise for Food Technology, 21-23 September 2012, International Workshop on Nanotechnology, Dhaka, Bangladesh.
Oral Development of a myoglobin immunosensor based on mixed self-assembled monolayer, 25-26 June 2006, Postgraduate symposium, Faculty of Biological Science, University of Leeds, UK Development of a myoglobin impedimetric immunosensor based on self-assembled monolayer onto gold, 25-27 June 07, Postgraduate symposium, Faculty of Biological Science, University of Leeds, UK Designed affinity surfaces permitting Biosensor self-assembly, 1-4 April 2007, XIXth International Symposium on Bioelectrochemistry and Bioenergetics, Toulouse, France Affinity directed construction of amperometic biosensors and impedimetric reagentless immunosensors - letting the sensor construct itself, 7-12 September 2008, the 59th Annual Meeting of the International Society of Electrochemistry (Electrochemistry down to the molecular level: interfacial science for life and technology), Seville, Spain Development of strip-based immunoassay for rapid, onsite and cost-effective diagnosis of different Foot-and-Mouth Disease (FMD) virus serotypes in livestock, 22 April 2011, Bangladesh Agricultural Research Council (BARC) workshop, Dhaka, Bangladesh Insight into INSPIRE-BGE, Khulna University Project, 4 August 2011, National Seminar on Bioprospecting of Medicinal Plants in the Sundarbans, Khulna University, Khulna, Bangladesh Tethering sensing molecules at nano-scale surfaces for medical applications, 2 March 2012, International Postdoctoral Symposium Series, Kulliyyah of Science, International Islamic University Malaysia (IIUM), Kuantan, Malaysia. Tethering Surface at Nano-scale for Point-of-care Monitoring, 14 July 2012, Green Chemistry for Sustainable Development, Jessore Science and Technology University, Jessore, Bangladesh Association of Vitamin D Receptor Gene Bsm1 (A>G) And Fok1 (C>T) Polymorphism in the Pathogenesis of Impaired Glucose Tolerance in a Bangladeshi Subjects, 14 July 2012, Green Chemistry for Sustainable Development, Jessore Science and Technology University, Jessore, Bangladesh Study on Bioactivity of Selected Shrubs of the Sundarbans, 14 July 2012, Green Chemistry for Sustainable Development, Jessore Science and Technology University, Jessore, Bangladesh.
4 | Page ACHIEVEMENTS
Scholarships
University Merit Scholarships for ranking 1st position throughout academic years of 4 year Bachelor of Science in Biotechnology
Overseas Research Students Award Scheme (ORSAS) (2004-2008) for pursuing PhD in Biochemistry and Microbiology at Institute of Membrane and Systems Biology (IMSB), University of Leeds, UK
Tetley and Lupton Scholarship (2004-2008) at Institute of Membrane and Systems Biology (IMSB), University of Leeds, UK
Emma and Leslie Reid Scholarship (2004-2008) Institute of Membrane and Systems Biology (IMSB), University of Leeds, UK
Isalmic Development Bank Merit Scholarship for Higher Technology (2016-2017) for pursuing Postdoctoral Research at Institute of Quantitative Biology, Biotechnology in The University of Edinburh, UK
Research Funding
International Development Research Centre (IDRC), Canada small grants for innovative research and knowledge sharing worth of CAD$ 60,000 for a project on Detection of Pathogens in Drinking Water to Prevent Waterborne Disease Transmission in Developing Countries in collaboration with University of British Columbia, Canada
Two research grants worth of $ 2000 each funded Khulna University Research Cell for performing High Performance Liquid Chromatography (HPLC) with methanolic extracts of mangrove plant species
Strategic Partnership Award worth of £45,000 to explore the indigenous and unique medicinal plants of the Sundarbans, the world’s largest mangrove forest, in search for new medicine as Principal Investigator
INSPIRE Exploratory Grant from British Council to explore the possibilities of collaboration of Biotechnology and Genetic Engineering Discipline, Khulna University, Bangladesh with different UK Higher Education Institutes (HEI)
Ministry of Science, Communication and Information Technology, Bangladesh grant worth of £10,000 to study genetic variability of livestock population as Co-Investigator
Comprehensive Disaster Management Programme (CDMP) under United Nations Development Programme grant of £15,000 for training biotechnology graduates to meet challenges of man-made and natural disasters as Associate Course Coordinator
5 | Page Seminars, Workshop and Diploma Organized
Organized an international conference on Updates on Natural Products in Medicine and Healthcare Systems on 06 July, 2013 at CSS Ava Center, Ruspha Strand Road, Khulna, Bangladesh as the convener of the event
Organized a national seminar on Mainstreaming the Medicinal Plant Research in Bangladesh: the Need for an Integrated and Systematic Approach on 26 November, 2012 Khulna University, Bangladesh as the convener of the event
Organized a national seminar on Bioprospecting of Medicinal Plants in the Sundarbans on 04 August, 2011 in Khulna University, Bangladesh as the convener of the event
Organized an interuniversity seminar on Ethical, Moral and Technological Implications of Reproductive and Therapeutic cloning on 21 June, 2011 in Khulna University, Bangladesh as the convener of the event
Coordinated a 14-week Postgraduate Diploma on Disaster Management Education for Biotechnology and Genetic Engineering Graduates
Organized an Expert consultation workshop on defining the needs of revised curricula of disaster management education for Biotechnology and Genetic Engineering Graduates 22 March, 2010 in Khulna University
MEMBERSHIPS
Member and Facilitator, American Society of Microbiology (ASM), USA
Member, Global Network of Bangladeshi Biotechnologist (GNOBB), Bangladesh
Member, Young Biotechnologists of Bangladesh
Member, American Chemical Society
Member, Bangladesh Chemical Society
SELECTED PUBLICATIONS
Reviews
Rony IK, Baten A, Bloomfield JA, Islam ME, Billah MM, Islam KD (2015) Inducing pluripotency in vitro: recent advances and highlights in induced pluripotent stem cells generation and pluripotency reprogramming, Cell Proliferation 48(2):140–156 (DOI: http://onlinelibrary.wiley.com/doi/10.1111/cpr.12162)
Shilpi JA, Islam ME, Billah M, Islam KMD, Sabrin F, Uddin SJ, Nahar L, Sarker SD (2012) Antinociceptive, Anti-Inflammatory, and Antipyretic Activity of Mangrove Plants: A Mini Review, Advances in Pharmacological Sciences, Volume 2012, Article ID 576086, 7 pages (DOI:
6 | Page http://dx.doi.org/10.1155/2012/576086)
Millner PA, Hays HCW, Vakurov A, Pchelintsev NA, Billah MM, Rodgers MA (2009) Nanostructured transducer surfaces for electrochemical biosensor construction—Interfacing the sensing component with the electrode, Seminars in Cell & Developmental Biology 20: 34-40 (DOI: http://dx.doi.org/10.1016/j.semcdb.2009.01.011).
Ahasan MM, Billah MM, Hasan MM, Islam KMD, Shilpi JA (2004) Transmission, biochemical manifestation and CD4+ cell count of HIV: a review, Pakistan Journal of Biological Sciences 7(2): 292-300 (DOI: http://dx.doi.org/10.3923/pjbs.2004.292.300).
Original Research Articles Islam MR, Mondol OK, Rahman MS, Billah MM, Rahman MS, Zohora US (2016) Screening of α-amylase producing bacteria from tannery wastes of Hazaribag, Bangladesh, Jahangirnagar University Journal of Biological Sciences 5(2):1-10 (http://dx.doi.org/10.3329/jujbs.v5i2.32511)
Afrin S, Pervin R, Sabrin F, Sohrab MH, Rony SR, Islam ME, Islam KD, Billah MM (2016) Assessment of Antioxidant, Antibacterial and Preliminary Cytotoxic Activity of Chloroform and Methanol Extracts of Casesalpinia crista Leaf, Bangladesh Journal of Botany 45(5): 1061-1068.
Pervin R, Afrin S, Sabrin F, Zohora US, Rahman MS, Islam KD, Billah MM (2016) Antioxidant, Antibacterial and Brine Shrimp Lethality Bioassay of Amoora cucullata, a Mangrove Plant. Journal of Young Pharmacists 2016; 8(1):33-8 (DOI: http://dx.doi.org/10.5530/jyp.2016.1.8).
Afrin S, Pervin R, Sabrin F, Rony SR, Sohrab MH, Islam ME, Islam KD, Billah MM (2016) In Vitro Antioxidant Activity, Antimicrobial and Preliminary Cytotoxic Activity of Cynometra ramiflora-a Mangrove Plant, Journal of Microbiology, Biotechnology and Food Sciences 6(2): 844-850 (DOI: http://dx.doi.org/10.15414/jmbfs.2016.6.2.844-850).
Islam T, Khan MIH, Afroz KF, Rahman MZ, Hossain MS, Islam ME, Billah MM, Islam KD (2016) Effective Identification of Lactobacillus paracasei ssp. paracasei-1 by 16S-23S rRNA Intergenic Spacer Region Sequencing, Journal of Microbiology, Biotechnology and Food Sciences 6(1): 695-701 (DOI: http://dx.doi.org/ 10.15414/jmbfs.2016.6.1.695-701).
Sahabuddin M , Ali H, Miah MF, Ferdous A, Akhter MS, Billah MM , Hassan Z, Ali L (2015) Association of vitamin D receptor gene Apa1 (G>T) and Taq1 (T>C) polymorphisms in the pathogenesis of impaired glucose tolerance in subjects of Bangladeshi origin, International Journal of Green and Herbal Chemistry 4(3): 357369.
Honi U, Sabrin F, Islam T, Islam ME, Billah MM, Islam KD (2013) Enzymatic activity and antibiotic resistance profile of Lactobacillus paracasei ssp. paracasei-1 isolated from regional yogurts of Bangladesh, Journal of Microbiology, Biotechnology and Food Sciences, 3(3):235- 239.
Saha S, Shilpi JA, Mandal H, Gofur MR, Billah MM, Nahar L, Sarker SD (2013) Bioactivity studies on Musa seminifera Lour. Pharmacognosy Magazine, 9: 315-322 (DOI:
7 | Page http://dx.doi.org/10.4103/0973-1296.117827).
Billah MM, Hays HCW, Hodges C, Ponnambalam S, Vohra R, Millner PA (2012) Mixed self- assembled monolayer (mSAM) based impedimetric immunosensors for cardiac troponin I (cTnI) and soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1), Sensors and Actuators B: Chemical 173: 361-366 (DOI: http://dx.doi.org/10.1016/j.snb.2012.07.017).
Pervin R, Sabrin F, Billah MM, Islam KMD, Elmezughi J, Shilpi JA (2012) Antibacterial and cytotoxic activity of Meyna spinosa stem, Turkish Journal of Pharmaceutical Sciences 9(2): 225- 230.
Chowdhury A, Hossain MN, Mostazir NJ, Fakruddin M, Billah MM, Ahmed MM (2012) Screening of Lactobacillus spp. from buffalo yoghurt for probiotic and antibacterial activity, Journal of Bacteriology and Parasitology 3(8):156-161 (http://dx.doi.org/10.4172/2155- 9597.1000156).
Sabrin F, Khatun MA, Alam SMN, Islam KD, Billah MM (2012) Antibacterial activity of chloroformic exytracts of Xylocarpus Mekongenesis (Lamk.) M. Roem. and Cerbera manghas, Journal of Innovation and Development Strategy 6(2): 13-18.
Kabir SMT, Sabrin F, Hasan MN, Islam KD, Billah MM (2012) Investigation of preliminary cytotoxic activity of ethanolic stem and leaf extracts of Amoora cucullata, Journal of Innovation and Development Strategy 6(2): 24-28.
Hoque MM, Sabrin F, Alam SMN, Khatun MA, Billah MM (2012) Cytotoxic activity of chloroformic exytracts of Xylocarpus Mekongenesis (Lamk.) M. Roem. and Cerbera manghas, Journal of Innovation and Development Strategy 6(2): 29-34.
Ferdoushi A, Sabrin F, Hasan MN, Islam KD, Billah MM (2012) Antibacterial activity of methanolic extracts of the leaves and stem of Amoora cucullata, Journal of Innovation and Development Strategy 6(2): 35-38.
Islam T, Sabrin F, Islam ME, Billah MM, Islam KMD (2012) Analysis of antimicrobial activity of Lactobacillus paracasei ssp. paracasei-1 isolated from regional yogurt, International Research Journal of Applied Life Sciences 1(4): 80-89.
Sabrin F, Hasan MN, Rahman MM, Islam KD, Billah MM (2011) Investigation on antimicrobial activities of the two selected shrubs from the Sundarbans (Clerodendrum inerme and Caesalpinia crista), Journal of Innovation and Development Strategy 5(2): 62-69.
Billah MM, Hodges C, Hays HCW, Millner PA (2010) Directed immobilisation of reduced antibody fragments onto a novel SAM on gold for myoglobin impedance immunosensing, Bioelectrochemistry 80(1): 49-54 (DOI: http://dx.doi.org/10.1016/j.bioelechem.2010.08.005).
Hoque MZ, Akter F, Hossain KM, Rahman MSM, Billah MM, Islam KMD (2010) Isolation, Identification and Analysis of Probiotic properties of Lactobacillus Spp. from selective regional yoghurts, World Journal of Dairy & Food Sciences 5(1): 39-46
Billah MM, Hays HCW, Millner PA (2008) Development of a myoglobin impedimetric
8 | Page immunosensor based on mixed self-assembled monolayer onto gold, Microchimica Acta 160(4): 447-454 (DOI: http://dx.doi.org/10.1007/s00604-007-0793-0).
Millner P, Vakurov A, Hays H, Pchelintsev N, Billah M, Rodger M, Gibson T (2007) The nanoscale design of surfaces for building biosensors, Journal of Biotechnology 131 (2): S127-128 (DOI: http://dx.doi.org/10.1016/j.jbiotec.2007.07.223).
Roberts LC, Hug SJ, Ruettimann T, Billah M, Khan AW, Rahman MT (2004) Arsenic removal with iron(II) and iron(III) in waters with high silicate and phosphate concentrations, Environment Science and Technology 38 (1): 307-315 (DOI: http://dx.doi.org/10.1016/10.1021/es0343205).
Shilpi JA, Uddin SJ, Rouf R, Billah MM (2004) Central nervous system depressant activity of Diospyros peregrina bark, Oriental Pharmacy and Experimental Medicine, 4(4) : 249-252
Ahamed T, Hossain KM, Billah MM, Islam KMD, Ahasan MM, Islam ME (2004) Adaptation of Newcastle disease virus (NDV) on Vero cell line, International Journal of Poultry Science 3(2): 153-156 (DOI: http://dx.doi.org/10.3923/ijps.2004.153.156).
Mamun MM, Billah MM, Ashek A, Ahasan MM, Hossain MJ, Sultana T ( 2003) Evaluation of Diuretic Activity of Ipomoea aquatica (Kalmisak) in Mice Model Study, Journal of Medical Sciences 3(5): 395-400 (DOI: http://dx.doi.org/10.3923/jms.2003.395.400).
Islam KMD, Ahamed T, Ahasan MM, Billah MM, Islam ME, Hossain KM (2003) Adaptation of Reovirus on Vero Cell Line, Journal of Animal and Veterinary Advances 2 (8): 425-429 (DOI: http://medwelljournals.com/abstract/?doi=javaa.2003.425.429).
Shrestha P, Ahasan MM, Islam KMD, Billah MM, Islam ME, Mehedi M, Mitra S, Islam MR (2003) Sero-prevalence of Infectious Bursal Disease Virus (IBDV) Specific Antibody in Chicken, Journal of Biological Sciences 6(14): 1234-1240 (DOI: http://dx.doi.org/10.3923/pjbs.2003.1234.1240).
Aryal P, Hossain KM, Ahasan MM, Islam KMD, Billah MM, Islam ME, Mehedi M, Mitra S (2003) Sero-prevalence of Newcastle Disease Virus (NDV) Specific Antibody in Chicken, Journal of Animal and Veterinary Advances 2 (6): 366-371 (DOI: http://medwelljournals.com/abstract/?doi=javaa.2003.366.371).
REFERENCES
Paul A Millner Professor of Bionanotechnology & Director of School School of Biomedical Sciences Faculty of Biological Sciences LIGHT Building University of Leeds Leeds LS2 9JT UK tel. +44 113 343 3149 direct/ 4272 (Mrs Debbie Jones, PA) email [email protected]
9 | Page Prof Satya Sarker BPharm (Hons) MPharm PhD FHEA Director of School School of Pharmacy & Biomolecular Sciences Liverpool John Moores University Byrom Street, Liverpool L3 3AF England, UK Phone: +44 151 231 2096 email [email protected]
Dr. S M Mahbubur Rahman Professor and Head Biotechnology and Genetic Engineering Discipline Khulna University Khulna-9208 Bangladesh tel. +880 41 2831546 email [email protected]
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