EPHA8 (Human) Recombinant Summary: This gene encodes a member of the receptor subfamily of the protein- family. EPH and EPH-related receptors have been Catalog Number: P5547 implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH Regulation Status: For research use only (RUO) subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 Product Description: Human EPHA8 (NP_065387.1, fibronectin type III repeats. The ephrin receptors are 571 a.a. - 924 a.a.) partial recombinant protein with GST divided into 2 groups based on the similarity of their tag expressed in baculovirus infected Sf21 cells. extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The protein Host: Insect encoded by this gene functions as a receptor for , A3 and A5 and plays a role in short-range Theoretical MW (kDa): 67 contact-mediated axonal guidance during development of the mammalian nervous system. [provided by RefSeq] Applications: Func, SDS-PAGE (See our web site product page for detailed applications information)

Protocols: See our web site at http://www.abnova.com/support/protocols.asp or product page for detailed protocols

Form: Liquid

Preparation Method: Baculovirus infected insect cell (Sf21) expression system

Purification: Glutathione sepharose chromatography

Purity: 89 % by SDS-PAGE/CBB staining

Activity: The activity was measured by off-chip mobility shift assay. The was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000. Substrate: Blk/Lyntide. ATP: 100 uM.

Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Storage Instruction: Store at -80°C. Aliquot to avoid repeated freezing and thawing.

Entrez GeneID: 2046

Gene Symbol: EPHA8

Gene Alias: EEK, HEK3, KIAA1459

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