Anti Human ARHGAP4 Polyclonal Antibody 0

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URL: www.sceti.jp/export/ E-mail: [email protected] KB538 For research use only Anti Human ARHGAP4 Polyclonal Antibody 0 Code No. KB538 Terget ARHGAP4 Category Signal transduction Gene ID 393 Primary Source HGNC:674 Synonyms C1; RGC1; p115; RhoGAP4; KIAA0131; ARHGAP4 [WB] human placenta tissue lysate Type Polyclonal Antibody Immunogen Recombinant protein of full length Human ARHGAP4 Raised in Mouse Myeloma - Clone number - Purification Protein A purified [WB] ARHGAP4 transfected 293T cell lysate Source Mouse Serum Isotype - (■) Negative control Cross Reactivity - (■) Transfectant Label Unlabeled Concentration 1 mg/mL Contents（Volume） 50 μg Buffer PBS, pH 7.2 [FCM] ARHGAP4 expressing 293 cells Storage Store at - 20 ℃ long term, store at 4 ℃ short term. Avoid repeated freeze-thaw cycles. Application WB,FCM ELISA WB IHC ICC - 1.0 - - IP FCM IF Neutralization - 1.0 - - (μg/mL) 0 Reference 1. Tribioli C., et al. "An X chromosome-linked gene encoding a protein with characteristics of a rhoGAP predominantly expressed in hematopoietic cells." Proc. Natl. Acad. Sci. U.S.A. 93:695-699(1996) 2. Nagase T., et al. "Prediction of the coding sequences of unidentified human genes. IV. The coding sequences of 40 new genes (KIAA0121-KIAA0160) deduced by analysis of cDNA clones from human cell line KG-1." DNA Res. 2:167-174(1995) 3. Tribioli C., et al. "Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N- acetyl transferase of Saccharomyces cerevisiae." Hum. Mol. Genet. 3:1061-1068(1994) UniPlot Summary //Function: Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells. //Subcellular location: Cytoplasm. Note: Just below the plasma membrane. //Tissue specificity: Predominantly in hematopoietic cells (spleen, thymus and leukocytes); low levels in placenta, lung and various fetal tissues. //Sequence similarities: Contains 1 FCH domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain. 3-6-7 Kasumigaseki, Chiyoda-ku Tokyo 100-0013 JAPAN.

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Supplemental information Dissection of the genomic structure of the miR-183/96/182 gene. Previously, we showed that the miR-183/96/182 cluster is an intergenic miRNA cluster, located in a ~60-kb interval between the genes encoding nuclear respiratory factor-1 (Nrf1) and ubiquitin-conjugating enzyme E2H (Ube2h) on mouse chr6qA3.3 (1). To start to uncover the genomic structure of the miR- 183/96/182 gene, we first studied genomic features around miR-183/96/182 in the UCSC genome browser (http://genome.UCSC.edu/), and identified two CpG islands 3.4-6.5 kb 5’ of pre-miR-183, the most 5’ miRNA of the cluster (Fig. 1A; Fig. S1 and Seq. S1). A cDNA clone, AK044220, located at 3.2-4.6 kb 5’ to pre-miR-183, encompasses the second CpG island (Fig. 1A; Fig. S1). We hypothesized that this cDNA clone was derived from 5’ exon(s) of the primary transcript of the miR-183/96/182 gene, as CpG islands are often associated with promoters (2). Supporting this hypothesis, multiple expressed sequences detected by gene-trap clones, including clone D016D06 (3, 4), were co-localized with the cDNA clone AK044220 (Fig. 1A; Fig. S1). Clone D016D06, deposited by the German GeneTrap Consortium (GGTC) (http://tikus.gsf.de) (3, 4), was derived from insertion of a retroviral construct, rFlpROSAβgeo in 129S2 ES cells (Fig. 1A and C). The rFlpROSAβgeo construct carries a promoterless reporter gene, the β−geo cassette - an in-frame fusion of the β-galactosidase and neomycin resistance (Neor) gene (5), with a splicing acceptor (SA) immediately upstream, and a polyA signal downstream of the β−geo cassette (Fig.
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ARHGAP4 Is a Novel Rhogap That Mediates Inhibition of Cell Motility and Axon Outgrowth ⁎ D.L
www.elsevier.com/locate/ymcne Mol. Cell. Neurosci. 36 (2007) 332–342 ARHGAP4 is a novel RhoGAP that mediates inhibition of cell motility and axon outgrowth ⁎ D.L. Vogt,a C.D. Gray,a W.S. Young III,b S.A. Orellana,c,d and A.T. Malouf a,c, aDepartment of Neurosciences, Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106, USA bThe Section on Neural Gene Expression, NIMH, NIH, DHHS, Bethesda, MD 20892, USA cDepartment of Pediatrics, Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106, USA dDepartment of Physiology and Biophysics, Case Western Reserve University, 11100 Euclid Ave., Cleveland, OH 44106, USA Received 5 January 2007; revised 30 May 2007; accepted 3 July 2007 Available online 24 July 2007 This report examines the structure and function of ARHGAP4, a novel hydrolysis of GTP to GDP which switches the GTPases to their RhoGAP whose structural features make it ideally suited to regulate “off” state, thereby inhibiting the downstream signaling that the cytoskeletal dynamics that control cell motility and axon out- regulates actin filament dynamics and motility. This increase in growth. Our studies show that ARHGAP4 inhibits the migration of GTP hydrolysis is dependent on a highly conserved arginine residue NIH/3T3 cells and the outgrowth of hippocampal axons. ARHGAP4 in the GAP domain that directly interacts with the active region of contains an N-terminal FCH domain, a central GTPase activating GTPases (Moon and Zheng, 2003; Li et al., 1997; Nassar et al., 1998; (GAP) domain and a C-terminal SH3 domain. Our structure/function Wittmann et al., 2003; Gamblin and Smerdon, 1998).
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C Loning of Rat ARHGAP4/C1, a Rhogap Family Member Expressed in the Nervous System That Colocalizes with the Golgi Complex and Microtubules Victoria C
Molecular Brain Research 107 (2002) 65–79 www.elsevier.com/locate/molbrainres Research report C loning of rat ARHGAP4/C1, a RhoGAP family member expressed in the nervous system that colocalizes with the Golgi complex and microtubules Victoria C. Folettaa,1 , Fraser D. Brown b , W. Scott Young III a,* aSection on Neural Gene Expression, The National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892, USA bLaboratory of Cellular Biology, The National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA Accepted 27 August 2002 Abstract The Rho GTPase family of intracellular molecular switches control multiple cellular functions via the regulation of the actin cytoskeleton. Increasing evidence implicates a critical involvement of these molecules in the nervous system, particularly during neuronal migration and polarity, axon and growth cone guidance, dendritic arborization and synaptic formation. However, the molecules regulating Rho GTPase activities in the nervous system are less known. Here, we present the cloning of rat ARHGAP4, a member of the Rho GTPase activating protein family, and also demonstrate its close linkage to the vasopressin 2 receptor gene. In vitro, recombinant ARHGAP4 stimulated the GTPase activity of three members of Rho GTPases, Rac1, Cdc42 and RhoA. ARHGAP4 mRNA expression was observed in multiple tissues with marked expression throughout the developing and adult nervous systems. On closer analysis of protein levels, ARHGAP4 was significantly restricted to specific regions in the nervous system. These included the stratum lucidem in the CA3 area of the hippocampus, neuronal fibers in the ventral region of the brainstem and striatum, and in the cerebellar granule cells.
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Supplementary Materials
Supplementary Materials 1 Supplementary Figure S1. Expression of BPIFB4 in murine hearts. Representative immunohistochemistry images showing the expression of BPIFB4 in the left ventricle of non-diabetic mice (ND) and diabetic mice (Diab) given vehicle or LAV-BPIFB4. BPIFB4 is shown in green, nuclei are identified by the blue fluorescence of DAPI. Scale bars: 1 mm and 100 μm. 2 Supplementary Table S1 – List of PCR primers. Target gene Primer Sequence NCBI Accession number / Reference Forward AAGTCCCTCACCCTCCCAA Actb [1] Reverse AAGCAATGCTGTCACCTTC Forward TCTAGGCAATGCCGTTCAC Cpt1b [2] Reverse GAGCACATGGGCACCATAC Forward GGAAATGATCAACAAAAAAAGAAGTATTT Acadm (Mcad) [2] Reverse GCCGCCACATCAGA Forward TGATGGTTTGGAGGTTGGGG Acot1 NM_012006.2 Reverse TGAAACTCCATTCCCAGCCC Forward GGTGTCCCGTCTAATGGAGA Hmgcs2 NM_008256.4 Reverse ACACCCAGGATTCACAGAGG Forward CAAGCAGCAACATGGGAAGA Cs [2] Reverse GTCAGGATCAAGAACCGAAGTCT Forward GCCATTGTCAACTGTGCTGA Ucp3 NM_009464.3 Reverse TCCTGAGCCACCATCTTCAG Forward CGTGAGGGCAATGATTTATACCAT Atp5b [2] Reverse TCCTGGTCTCTGAAGTATTCAGCAA Pdk4 Forward CCGCTGTCCATGAAGCA [2] 3 Reverse GCAGAAAAGCAAAGGACGTT Forward AGAGTCCTATGCAGCCCAGA Tomm20 NM_024214.2 Reverse CAAAGCCCCACATCTGTCCT Forward GCCTCAGATCGTCGTAGTGG Drp1 NM_152816.3 Reverse TTCCATGTGGCAGGGTCATT Forward GGGAAGGTGAAGAAGCTTGGA Mfn2 NM_001285920.1 Reverse ACAACTGGAACAGAGGAGAAGTT Forward CGGAAATCATATCCAACCAG [2] Ppargc1a (Pgc1α) Reverse TGAGAACCGCTAGCAAGTTTG Forward AGGCTTGGAAAAATCTGTCTC [2] Tfam Reverse TGCTCTTCCCAAGACTTCATT Forward TGCCCCAGAGCTGTTAATGA Bcl2l1 NM_001289716.1
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ARHGAP4 IS a SPATIALLY REGULATED RHOGAP THAT INHIBITS NIH/3T3 CELL MIGRATION and DENTATE GRANULE CELL AXON OUTGROWTH by DANIEL L
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