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Translation 3985 • FISHERIES AND MARINE SERVICE Translation Series No. 3985 REF Electrophoretic spectra of isomerases, transferases and oxidoreductases of the starfish Patina pectinifera LIBRARY FISHERIES AND OCEANS BIBLIOTHÈQUE PÉCHES ET OCÉANS by G. P. Manchenko and O. L. Serov Original title: Elektroforeticheskie spektry izomeraz, transferaz i oksidoreduktaz morskoi zvezdy Patina pectinifera From: Biol. Morya (5): 57-60, 1976 Translated by the Translation Bureau (UDP) Multilingual Services Division Department of the Secretary of State of Canada Department of the Environment Fisheries and Marine Service Halifax Laboratory Halifax, N.S. 1977 ( 9 pages typescript (91e1)(3— 1 • DEPARTMENTOFTHESECRETARYOFSTATE SECRÉTARIAT D'ÉTAT TRANSLATION BUREAU BUREAU DES TRADUCTIONS • MULTILINGUAL SERVICES DIVISION DES SERVICES CANADA DIVISION MULTILINGUES TRANSLATED FROM - TRADUCTION DE INTO - EN Russian English AUTHOR - AUTEUR G. P. Manchenko and OY, L, Serov TITLE IN ENGLISH - TITRE ANGLAIS Electrophoretic Spectra of Isomerases, Transferases and Oxidoreductases of the Starfish Patiria-pectinifera, TITLE IN FOREIGN LANGUAGE (TRANSLITERATE FOREIGN CHARACTERS) TITRE EN LANGUE ÉTRANGÉRE (TRANSCRIRE EN CARACTÉRES ROMAINS) Elektroforeticheskie spektry izomraz,.transferaz i oksidoreduktaz morskoi zvezdy Patirià-Lpéctinifera. REFERENCE IN FOREIGN LANGUAGE (NAME OF BOOK OR PUBLICATION) IN FULL. TRANSLITERATETOREIGN CHARACTERS , RÉFÉRENCE EN LANGUE ÉTRANGÉRE (NOM DU LIVRE OU PUBLICATION), AU COMPLET, TRANSCRIRE EN CARACTÉRES ROMAINS, Biologiya morya, REFERENCE IN ENGLISH - RÉFÉRENCE EN ANGLAIS Marine Biàlogy. PUBLISHER- ÉDITEUR PAGE NUMBERS IN ORIGINAL DATE OF PUBLICATION NUMÉROS DES PAGES DANS DATE DE PUBLICATION L'ORI GINAL Not given. 57-60 YEAR ISSUE NO. VOLUME PLACE OF PUBLICATION ANNÉE NUMÉRO NUMBER OF TYPED PAGES. ' LIEU DE PUBLICATION NOMBRE DE PAGES DACTYLOGRAPHIÉES USSR 1976 5 9 REQUESTING DEPARTMENT Environment TRANSLATION BUREAU NO. WNISTÉRE- CLIENT NOTRE DOSSIER NCI 1089221 Fisheries/Scientific Info. BRANCH OR DIVISION TRANSLATOR (INITIA LS) vie & _Publications Branch DIRECTION OU DIVISION TRADUCTEUR (INITIALES) PERSON REQUESTING Dr. P. H, Odense, Halifax Lab, P:i..A.NSLA:HON DEMANDÉ PAR i . Th Y) 0 n! ,1 YOUR NUMBER VOTRE DOSSIER NCI 5053-1 seurnDnt DATE OF REQUEST 2 March 1977 DATE DE LA DEMANDE 'APR -- 7 1971 503.200-10-6 (REV. 2/68 ) 7530-21-029-5333 Fd-)II it 3 g^s ,%cretary Secrétariat `- 'of 11,3tate d'État TRANSLATION BUREAU F3UREAU DES TRADUCTIONS MULTILINGUAL SERVICES DIVISION DES SERVICES DIVISION MULTILINGUES CLIENT'S NO. DEPARTMENT DIVISION/HRANCH CITY NO DU CLIENT MINISTLRE DIVISION/DIRECTION VILLE Fisheries/Scientific Info. 5053-1 Environment & Publications Branch Halifax, N. S. SUREAU NO. LANGUAGE TRANSLATOR(INITIALS) ND DU BUREAU LANGUE TRADUCTEUR ( INITIALES; 1089221 Russian WDP APR - 7 1977 Biologiya morya (Marine Biology), No, 5, pp, 57-60? 1976 (USSR) ^^^i:ÉJ?? '3^ :.;.*' ?QÇf. ;.Nntd UDC 593,93+577.15 's°or Biochemistry ^lèctrôphôrètic Spectra Of Isonièràse$; Trà^is^èràsès and .(?xidoreductases.of .the .Starfish-Td-tirià ^ièctiriifèra ^-^-^--- By G. P. Manchenko and 0, L. Serov, Genetics Laboratory, Institute of Marine Biologÿ, Far Eastern Science Center, Academy of Sciences USSR, Vladivostok 690022, and the Laboratory of Genetic Fundamentals of Ontogeny, Institute of Cytology and Genetics, Siberian Branch, Academy of Sciences, USSR, Novosibirsk 630090 Using the method of vertical starch gel electrophoresis we have /57* derived and described the spectra of 10 enzymes from different organs of the starfish-Patiria pectinifera: glucosophosphatisomerase, phosphoglucomutase, adenineat^ cinâse, hexokinase, oXidase of tetrazolium, xanthindehydrogenase, a - glycerophosphatedehydrogenase, 6 = phosphogluconatedehydrogenase, glucoso-6-phosphatedehydrogenase and malate dehydrogenase In pôpulation genetics wide use is made of biochemical features found by the methdd of electrophoresis in gels (Habby and Lewontfin, 1966 ; Altukhov, 1973, and others) The Mendelizing, as a rule monogenetic, features of a protein nature,are-a direct result of the activity of individual genes. Numbers in the right-hand margin indicate the correspondzng pages in the original. -Trarislàtôr's-'note. The foregoing names have all been takenndirectly from the or'iginal Russiâ.n; hence spellings may in individual cases vary some- what from the standard orthography both here and elsewhere in this translation. SEC 5-25T (6/76) 2 Polymorphic and monomorphic proteins are used not ânly in population, but also in taxonomic, research on different groups of animals. Dhanani and Kitto (1970). as well as Schopf and Murphy (1973)y showed that it is advisable to use biochemical features when resolving problems involving the macro- an.dd microevolution of echinoderms. Moore and Villéé (1963) have studied the electrophoretic spectra of multiple forms of malate dehydrogenase in embryos of the starfish Asteria.s ^forbesi. Dhanani and Kitto (1970) and Schopf and Murphy (1973) have described a series of enzymes found with starch gel electrophoresis in some species of starfish, This paper describes the electro- phoretic spectra of 10 enzymes of the starfish Patiria pectinifera. Materials and Methods In our study we used 26 starfish of Patiniâ. Tectinifèra (Mûller et , .3 .. Troschel), collected in November in Ussuri Gulf of the Sëà of Japan (Lazurnaya Bay). We studied the ambulacral feet, stomachs,lliver processes and gonads of both live animals and specimens stored at a temperature of -55°C for 60 days. We got extracts of the organs by homogenizing them in an equal amount of deionized water and centrifuging the homogenates at 18,000 g for 1 hour. We used the supernatant for electrophoresis, All of the operations involved in deriving the extracts, were carried out in the cold. We performed electrophoresis in a 14% starch gel in a discontinuous ^e buffer system ( BS-1): gel buffer - 0.05M tris-0.002M EDTA -Na2 - boric acid, pH 7.9; electrode7-,buffer - 0.3M boric acid - NaOH, pH 8.1, after Serov (Serov, 1973), as well as in continuous buffer systems: 1) gel buffer - 0.01M phosphate, pH 6.8; electrode buffer - 0.2M phosphate, pH 6,,^J BS-II); -Tràrislàtor's note. Expands to ethylenédiaminetetraacetic acid. I • 3 2) gel buffer - 0.01M tris - 0.0029M citric acid, pH 7.0; electrode buffer - 0.155M tris - 0.043M citric acid, pH 7,0 (BS-III), after Shaw and Prasad (1970). When we finished the electrophoresis we cut the gel into plates 2 mm thick and found the zones of enzyme activity using the standard histochemical colors after Shaw and Prasad (1970); we found tetrazolium oxidase after Breyer (1967). Results and Discussion We got electrophoretic spectra of the following enzymes: /5 8 glucosophosphatisomerase, phosphoglucomutase, adenylatkinase, hexokinase, oxidase of tetrazolium, xanthindehydrogenase, a - glycerophosphatedehydro- genase, 6 - phosphogluconatedehydrogenase, glucoso-6-phosphatedehydrogenase and malate dehydrogenase. Glucosophosphatisomerase (GPhI). The enzyme spectrum from the stomach, the liver processes and the ambulacral feet during electrophoresis in BS-1 was represented by one zone (Fig. 1, 2, see inset I), which had the same mobility for all organs. The most intensively colored GPhI zones were [those of] the stomach and ambulacral feet. When we stored the organs in a frozen state, and the extracts at a temperature of 3°C, the activity of the enzyme dropped considerably or disappeared altogether, Phosphoglucomutase (PhGM), During electrophoresis of freshly prepared extracts of the stomach, ambulacral feet and gonads in BS-III we found one zone of activity of PhGM (Fig. 2, 5; see inset I) with the same mobility for all organs. We found no enzyme activity in the liver processes. We noted a conSiderable reduction in PhGM activity for organs that had been stored in a frozen state. 4 Adenylatkinase (AK), The enzyme spectrum of the stomach, obtained in BS-III, was represented by one zone of activity (Fig. 2, 1). The same spectrum was characteristic of the ambulacral feet and livér processes, but the AK activity of the liver processes was much lower than in the other organs. Storage of the organs in a frozen state led to a reduction in the enzyme activity, while a double freezing/thawing procedure brought about the complete disappearance of such activity, Hexokinase (HK). The electroîshoretic HIC spectrum of different organs was represented by two zones, of which one (the fast-migrating) was monomorphic for all organs, while the second (the slow-migrating) varied in different organs with regard to mobility and intensity of coloring (Fig. 2, 2 and 3). Enzyme activity did not drop when the organs were stored in a frozen state, but it was completely lost after a double freezing and thawing prôcedure. --Oxidase of tetrazolium.--(0T), The OT spectrum of liver processes found during electrophoresis in BS-1 was represented by two zones (Fig. 1, 4). In the stomach and the ambulacral feet under these conditions we found two analogous zones, but the activity of the fast-migrating OT was much lower than in the case of the liver processes. We found high OT activity in freshly prepared extracts. The storagé of organs in a frozen state, and of extracts at a temperature of 3 °C, reduced enzyme activity. -Xanthindehydrogenase (XDH), In each of the organs that we investigated we found one zone of enzyme activity with the same mobility. We noted the greatest XDH activity in the stomach and liver processes (Fig. 1, 3), Enzyme acitivity was much lower ih the gonads and in th ë ambulacral feet.
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