(Syllepte) Derogata (Fabricius, 1775) (Lepidoptera: Crambidae) Under Laboratory Conditions
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Dannon et al., J. Appl. Biosci. 2021 Efficacy of Beauveria bassiana against the cotton leaf roller, Haritalodes (Syllepte) derogata (Fabricius, 1775) (Lepidoptera: Crambidae) under laboratory conditions. Journal of Applied Biosciences 157: 16254 - 16266 ISSN 1997-5902 Efficacy of Beauveria bassiana against the cotton leaf roller, Haritalodes (Syllepte) derogata (Fabricius, 1775) (Lepidoptera: Crambidae) under laboratory conditions. Dannon H. Fabrice1,4,*, Douro Kpindou O. Kobi4, Toffa Mehinto Joelle3, Zantchedji D. M. Désiré1, Zinsou A. Valerien1, Dannon A. Elie2,4, Houndété A. Thomas5, Elégbédé I. A. Maurille T.1, Olou B. Dénis6 1Laboratoire de Phytotechnie, d’Amélioration et de Protection des Plantes (LaPAPP), Faculté d’Agronomie (FA), Université de Parakou, Parakou, Bénin 2Ecole Normale Supérieure (ENS), Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques d’Abomey, Natitingou, Bénin 3Faculté des Sciences et Techniques (FAST), Université Nationale des Sciences, Technologies, Ingénierie et Mathématiques, Dassa-Zoumè, Bénin 4Institut International d’Agriculture Tropicale, 08 BP 0932 Tri Postal, Cotonou, Benin. 5Centre de Recherches Agricoles Coton et Fibres (CRA-CF), Institut National de Recherches Agricoles, Bohicon, Bénin 6Programme Analyse de la Politique Agricole, Centre de Recherches Agricoles, Institut National de Recherches Agricoles, (PAPA/CRA-A/INRAB), Agonkanmey, Bénin *Corresponding author : Dannon Houénoudé Fabrice, Contact : (+229) 95274027, e-mail : [email protected] Original submitted in on 16th October 2020. Published online at www.m.elewa.org/journals/ on 31st January 2021 https://doi.org/10.35759/JABs.157.10 ABSTRACT Objective: The leaf-roller caterpillar Haritalodes (=Syllepte) derogata (Fabricius, 1775) (Lepidoptera: Crambidae) induces high yield losses by damaging cotton leaves and reducing the photosynthetic activity of the plant. Laboratory bioassays were carried to evaluate the effect of Beauveria bassiana on the survival of H. derogata larvae. Methodology and results: In the first trial, screening of thirteen B. bassiana isolates was performed on third larval instars at 107 conidia.mL-1. In the second trial, effects of five concentrations (105 to 109 conidia.mL-1) of the three best isolates of the fungus were tested. Conidia suspension was applied on each larva topically. Germination rates of conidia used varied between 90.2% to 95.7%, 24 hours after incubation. Five isolates were found to be the most promising namely Bb116, Bb3, Bb11, Bb6 and Bb115. In the second bioassay, caterpillar mortality increased with fungal concentration. Lethal Concentration (LC50) was estimated to 1.18x1015 conidia.mL-1, 1.75x1013 conidia.mL-1, 1.75x1013 conidia.mL-1, 9 days after inoculation for Bb3, Bb11 and Bb115, respectively. Conclusion and application of results: The use of B. bassiana as a biopesticide against H. derogata could be a good alternative method to control the pest. It is an environmentally friendly method with less side effects compared to the application of synthetic pesticides on cotton. This method could be tested in future station and field experiments. Keywords: Cotton, Integrated pest management, Haritalodes (=Syllepte) derogata, Beauveria bassiana, Lethal Concentration. 16254 Dannon et al., J. Appl. Biosci. 2021 Efficacy of Beauveria bassiana against the cotton leaf roller, Haritalodes (Syllepte) derogata (Fabricius, 1775) (Lepidoptera: Crambidae) under laboratory conditions. INTRODUCTION Cotton crop is the first cash crop of Benin that in the value chain and insect resistance mainly of contributes up to 80% to official export earnings and Lepidopteran species became a major issue to be 13% to GDP (Great Development Product) (Afouda solved for boosting cotton production in Benin. et al., 2013). In 2018-2019 season, the recorded Among these caterpillars of cotton, H. derogata production was 700,000 TM ranking Benin as the (Fabricius, 1775) (Lepidoptera: Crambidae) largest producer in West Africa (Tonavoh, 2019). inducing up to 20-60% of cotton yield loss (Silvie, This increase in cotton production is related to the 1993). Thus, this phyllophagous could alter the expansion of cultivated areas and the use of new photosynthetic activity of the plant. In order to cotton varieties but not to an increase in yield (CRA- economically and ecologically manage cotton insect CF 2018). Yield is heavily limited by pressure from pests, biological control remains an attractive insect pests. In West Africa, insect pests become a option. This control option includes the use of major constraint on increasing cotton production natural enemies of target insect pest species. (Brevault et al., 2017). Indeed, cotton is the one of Several studies reported various natural enemies the most damaged crops with more than 1300 (parasitoids and pathogens) of H. derogata species of insect pests, heavily limiting its (Gahramanova et al., 2020). In this perspective, the productivity (UNCTAD, 2008 cited by Douro entomopathogen Beauveria bassiana (Bals.-Criv.) Kpindou et al., 2013). Among these, phyllophagous Vuill., 1912, is a promising candidate. Different and carpophagous caterpillars are the most isolates were identified infecting a wide range of destructive pests of cotton in Benin. There are two insects (707 species belong to 15 orders) and mites main groups of caterpillar pests according to their (13 species) (Zimmerman 2007; Lambert 2010). feeding preferences (Héma et al., 2009). The first The use of B. bassiana is an environmentally group consisted of defoliators such as the leaf-roller friendly control mean and harmless to human health caterpillar Haritalodes (=Syllepte) derogate F. compared to chemical pesticides (Althouse et al., (Crambidae)), the leaf-eating noctuids Anomis flava 1997; Faria and Wraight, 2001). Fungus kills both F. and Spodoptera littoralis (Boisduval). On the susceptible adult and immature stages (eggs, other hand the second group includes bollworms or larvae) causing the so-called ‘’white muscardine’’ fruit-feeders namely the old world bollworm disease by a simple contact. Pest mortality may Helicoverpa armigera (Hubner), the red bollworm occur with the conidia development process and Diparopsis watersi Rothschild and the spiny dead host becomes a new source of contamination. bollworms Earias insulana (Boisduval) and Earias Four steps are known for the infection of the fungus biplaga (Walker) which feed on fruiting organs namely adhesion, germination and differentiation, (squares, flowers, bolls) (Silvie et al., 2013). penetration, and dissemination within the host and Expansion of cultivated area led to higher to another host (Dannon et al., 2020). It is not consumption of chemical pesticides in order to theoretically possible for insects to develop overcome insect pests damage (Westerberg, 2017). resistance to B. bassiana because the fungus The side effects of chemical insecticide misuses simultaneously uses several modes of action such included the contamination of cotton production as infection by conidia and toxins (Mascarin and area, human hazards (frequent pesticide poisoning, Jaronski, 2016) and as a living organism, it can skin and stomach irritation), insect pest resurgence adapt to various host changes (Sabbahi, 2008). The and resistance, environmental pollution (Lawani et present study was designed to assess the al., 2017; Djihinto et al., 2016; Djihinto et al., 2009). susceptibility of H. derogata to various B. bassiana Cotton is an income generating for all stakeholders isolates under laboratory conditions. 16255 Dannon et al., J. Appl. Biosci. 2021 Efficacy of Beauveria bassiana against the cotton leaf roller, Haritalodes (Syllepte) derogata (Fabricius, 1775) (Lepidoptera: Crambidae) under laboratory conditions. MATERIALS AND METHODS Insects: Larvae of H. derogata were collected during the International Institute of Tropical Agriculture (IITA), period of 12 to 15 October 2017 on untreated cotton Benin station. They were fed using untreated cotton plants samples at the experimental site of the agricultural leaves. The first generation was used in the different research center, cotton and fiber (CRA-CF), in two bioassays. Only, the third larval instars were tested with regions of central Benin namely Savalou (07°54’.032’’N; B. bassiana isolates. 001°55’.024’’E; 179 m of altitude) and Savè Fungal isolates: Thirteen isolates were used in the first (08°00’.003’’ N; 002°25’.054’’E; 175 m of altitude). They trial and the best three ones were retained for the were reared at (26 ± 1) °C, (70 ± 5) % HR and a second experiment. All isolates were obtained from the photoperiod of 12:12 (L: D) h in the laboratory at collection unit of IITA-Benin (Table 1). Table 1: Information about B. bassiana isolates used in different trials. Range Abbreviation of fungal Register N° Host Auteur isolates (Country of origin. (Year of isolation) district) 1 Bb2 5644 Eldana sacharina -IITA-Benin (1997) (Benin, Atlantique) 2 Bb3 5645 Eldana sacharina -IITA-Benin (1997) (Benin, Atlantique)) 3 Bb5 5647 Acigona sp. - IITA-Benin (1997)- (Nigeria, Ikom) 4 Bb6 5648 Acigona sp. - IITA-Benin (1997)- (Benin, Alibori) 5 Bb11 5653 Sesamia calamistis -IITA-Benin (1997) (Benin, Atlantique)) 6 Bb115 I93-841 Locusta migratoria -Madagascar (1993) (Madagascar, Toliara) 7 Bb69 I91-623 Zonocerus variegatus -IITA-Benin (1991) (Benin, Atlantique) 8 Bb71 I91-592 Zonocerus variegatus -IITA-Benin (1991) (Benin, Atlantique) 9 Bb84 I91-679 Hieroglyphus -IITA-Benin (1998) (Benin, Alibori) 10 Bb353 - Callosobruchus