DOCSLIB.ORG

Comparing Host and Parasite Phylogenies: Gyrodactylus Flatworms Jumping from Goby to Goby

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Comparing Host and Parasite Phylogenies: Gyrodactylus Flatworms Jumping from Goby to Goby Syst. Biol. 54(5):710–718, 2005 Copyright c Society of Systematic Biologists ISSN: 1063-5157 print / 1076-836X online DOI: 10.1080/10635150500221036 Comparing Host and Parasite Phylogenies: Gyrodactylus Flatworms Jumping from Goby to Goby TINE HUYSE AND FILIP A. M. VOLCKAERT Katholieke Universiteit Leuven, Laboratory of Aquatic Ecology, Ch. De Beriotstraat´ 32, B-3000 Leuven, Belgium; E-mail: [email protected] (T.H.) Abstract.—The combination of exceptionally high species diversity, high host specificity, and a complex reproduction sys- tem raises many questions about the underlying mechanisms triggering speciation in the flatworm genus Gyrodactylus. The coevolutionary history with their goby hosts was investigated using both topology- and distance-based approaches; phylogenies were constructed of the V4 region of the 18S rRNA and the complete ITS rDNA region for the parasites, and 12S and 16S mtDNA fragments for the hosts. The overall fit between both trees was significant according to the topol- ogy-based programs (TreeMap 1.0, 2.0β and TreeFitter), but not according to the timed analysis in TreeMap 2.0β and the distance-based method (ParaFit). An absolute timing of speciation events in host and parasite ruled out the possibility of synchronous speciation for the gill parasites, favouring the distance-based result. Based on this information together with the biological background of host and parasite, the following TreeMap solution was selected. The group of gill parasites evolved from a host switch from G. arcuatus, parasitizing the three-spined stickleback onto the gobies, followed by several host-switching events among the respective goby hosts. The timing of these events is estimated to date back to the Late Pleistocene, suggesting a role for refugia-mediated mixing of parasite species. In contrast, it is suggested that cospeciation in the fin-parasites resulted in several host-associated species complexes. This illustrates that phylogenetically conserved host-switching mimics the phylogenetic signature of cospeciation, confounding topology-based programs. [Coevolution; Gobies; Gyrodactylus; host-parasite interactions; host-switching; speciation.] One of the most significant radiations of platyhelminth scribed as well (Bakke et al., 2002). As such, all speciation fish parasites is documented in the monogenean “super- modes appear plausible in this group. genus” Gyrodactylus (Cribb et al., 2002). The 409 Gyro- In spite of these interesting characteristics, together dactylus species named so far (Harris et al., 2004) are with the fact that Gyrodactylus may pose serious threats to expected to represent only 2% to 10% of the estimated aquaculture (e.g., the highly pathogenic salmon parasite diversity (Bakke et al., 2002). Gyrodactylus displays the G. salaris), quantitative host-parasite cospeciation stud- widest host range of any monogenean family (19 orders ies are lacking (Bakke et al., 2002). According to Page of bony fish), encompassing both highly specific and gen- et al. (1996), the prerequisites for coevolutionary stud- eralist species. These remarkable facts can be linked with ies are (1) the availability of a sound alpha taxonomy of their even more remarkable reproduction mode. Gyro- both host and parasite, (2) robust phylogenies of hosts dactylus spp. contain a fully grown daughter in utero, and parasites, (3) although not essential, molecular phy- which in turn encloses a developing embryo, boxed in- logenies are preferably based on homologous characters, side one another like Russian dolls; asexual, partheno- (4) wide taxon sampling, and (5) quantitative compari- genetic and sexual reproduction alternate (Cable and son of host and parasite trees by means of explicit sta- Harris, 2002). In optimal conditions, a first-born daugh- tistical tests. The first four prerequisites are fulfilled in ter can be produced within 24 hours of her parent’s birth our model consisting of the Gyrodactylus parasites living (Cable and Harris, 2002). This can result in explosive pop- on gobies of the genus Pomatoschistus. (1) The various ulation growths, and the direct life cycle (only one host Gyrodactylus taxa have been described by morphologi- involved) allows them to invade new habitats with their cal, morphometric, and molecular methods (Huyse and hosts. All these factors may accelerate population differ- Malmberg, 2004; Huyse et al., 2004a), whereas the host entiation and thus speciation. species have been extensively studied by Miller (1986). On the one hand, the direct life cycle and the high host- They occur in the Atlantic and Mediterranean coasts of specificity enforce a tight relationship of a Gyrodacty- Europe; some goby species are sympatric and hybridiza- lus species and its host, promoting coevolution (Poulin, tion has been reported (Webb,1980). (2) The phylogenetic 1992; Kearn, 1994). On the other hand, the ability to relationships of Gyrodactylus have been studied using the produce an entire population from a single individual complete ITS rDNA region and the V4 region of the 18S increases the chance for sympatric speciation and speci- rRNA (Huyse et al., 2003); the phylogeny of the host has ation by host-switching (Brooks and McLennan, 1993). been reconstructed from 12S and 16S mtDNA fragments, They may also survive for some time independently and in order to compare homologous characters. (3) The of their host and a “swimming behavior” has been ob- ITS 1 locus has been sequenced as well (Huyse et al., served (Cable et al., 2002). These features add to the 2004b). (4) In order to minimize sampling bias, sampling colonizing capability of Gyrodactylus, illustrated by sev- has been extended over time and space throughout the eral switches between fish families (Harris, 1993; Zietara natural geographical distribution of the fish hosts (see and Lumme, 2002) and even fish orders (Huyse et al., below). 2003). However, phylogenetic radiations (diversification All respective Gyrodactylus species display phylo- constrained/triggered by host phylogeny) have been de- genetic host specificity towards gobies of the genus 710 2005 HUYSE AND VOLCKAERT—COEVOLUTION: DISTANCE- VERSUS TOPOLOGY-BASED PROGRAMS 711 Pomatoschistus (Huyse et al., 2003). They can be split into position for all sequences was compared usinga5%χ 2 two groups by their genetic and ecological parameters. test on the average composition (TREE-PUZZLE v. 5.0, Group A represents a monophyletic group of closely re- Schmidt et al., 2002). Plotting transitions and transver- lated and host-specific species, mainly infecting gills, sions against divergence of the complete data set using all belonging to the subgenus G.(Mesonephrotus). Host DAMBE v. 4.0.75 (Xia and Xie, 2001) did not show sat- specificity is lower in group B, which comprises species uration. The molecular-clock hypothesis was tested as- that are dominantly found on fin and skin and belong to suming the HKY+ model with the likelihood-ratio test G. (Paranephrotus). In this article, we fulfill the last prereq- (LRT) for the clock hypothesis implemented in TREE- uisite for coevolutionary studies by comparing host and PUZZLE. ModelTest v. 3.06 (Posada and Crandall, 1998) parasite phylogenies using a combination of statistical was used to estimate the optimal model of molecular approaches. In addition, we test whether the difference evolution in a likelihood-testing framework. These pa- in niche is reflected in the evolution and distribution on rameters were used in the maximum likelihood (ML) the host species: Are exposed fin parasites more subject method using PAUP∗;trees were statistically tested by to host-switching than the more “sheltered” gill para- calculating P values for the individual branches. This sites? Did the monophyletic group of gill parasites evolve likelihood-ratio test assesses whether branch-lengths are through cospeciation with the host, as generally expected significantly different from zero by optimizing all branch for host-specific parasites (Poulin, 1992; Kearn, 1994)? lengths under the constraint that one of the branches is Alternatively, did the group of the more generalist fin zero, for each branch tested. For maximum parsimony parasites evolve through repeated host-switching events (MP) the exhaustive search method was performed us- instead of cospeciation? ing the branch and bound algorithm. In these analyses gaps were treated both as missing data and fifth character because indels might be phylogenetically informative; all MATERIAL AND METHODS sites were equally weighted. The neighbor-joining (NJ) Host and Parasite Data search was conducted (1000 replicates of tree-bissection During 1999 to 2002, 15 Gyrodactylus taxa were reconnection branch swapping) from a matrix of ML ge- collected on Gobiusculus flavescens and several Po- netic distances calculated under the optimized model. matoschistus species (Gobiidae, Teleostei) along the ML branch lengths have been generated under the spec- northeastern Atlantic continental shelf, the Baltic, Adri- ified model and topology, with and without a molec- atic, and Mediterranean Seas (see Huyse et al., 2003). ular clock assumption. Bayesian inference (BI) using Two Gyrodactylus parasites of the three-spined stickle- MrBayes v. 3.0b4 (Huelsenbeck and Ronquist, 2001) was back, Gasterosteus aculeatus, were collected as outgroup performed, using the model obtained by ModelTest. Pos- species (G. arcuatus and G. branchicus). Phylogenetic re- terior probabilities were estimated over 2.0 × 106 gen- lationships of the Gyrodactylus parasites were inferred erations,
Load more

Recommended publications
  • Ahead of Print Online Version Gyrodactylus Aff. Mugili Zhukov
    Ahead of print online version FoliA PArAsitologicA 60 [5]: 441–447, 2013 © institute of Parasitology, Biology centre Ascr issN 0015-5683 (print), issN 1803-6465 (online) http://folia.paru.cas.cz/ Gyrodactylus aff. mugili Zhukov, 1970 (Monogenoidea: Gyro- dactylidae) from the gills of mullets (Mugiliformes: Mugilidae) collected from the inland waters of southern Iraq, with an evalutation of previous records of Gyrodactylus spp. on mullets in Iraq Delane C. Kritsky1, Atheer H. Ali2 and Najim R. Khamees2 1 Health Education Program, school of Health Professions, idaho state University, Pocatello, idaho, UsA; 2 Department of Fisheries and Marine resources, college of Agriculture, University of Basrah, Basrah, iraq Abstract: Gyrodactylus aff. mugili Zhukov, 1970 (Monogenoidea: gyrodactylidae) is recorded and described from the gill lamellae of 11 of 35 greenback mullet, Chelon subviridis (Valenciennes) (minimum prevalence 31%), from the brackish waters of the shatt Al-Arab Estuary in southern iraq. the gyrodactylid was also found on the gill lamellae of one of eight speigler’s mullet, Valamugil speigleri (Bleeker), from the brackish waters of the shatt Al-Basrah canal (minimum prevalence 13%). Fifteen Klunzinger’s mullet, Liza klunzingeri (Day), and 13 keeled mullet, Liza carinata (Valenciennes), collected and examined from southern iraqi waters, were apparently uninfected. the gyrodactylids from the greenback mullet and speigler’s mullet were considered to have affinity toG. mu- gili Zhukov, 1970, and along with G. mugili may represent members of a species complex occurring on mullets in the indo-Pacific region. A single damaged gyrodactylid from the external surfaces of the abu mullet, Liza abu (Heckel), was insufficient for species identification.
    [Show full text]
  • Reference to Gyrodactylus Salaris (Platyhelminthes, Monogenea)
    DISEASES OF AQUATIC ORGANISMS Published June 18 Dis. aquat. Org. 1 I REVIEW Host specificity and dispersal strategy in gyr odactylid monogeneans, with particular reference to Gyrodactylus salaris (Platyhelminthes, Monogenea) Tor A. Bakkel, Phil. D. Harris2, Peder A. Jansenl, Lars P. Hansen3 'Zoological Museum. University of Oslo. Sars gate 1, N-0562 Oslo 5, Norway 2Department of Biochemistry, 4W.University of Bath, Claverton Down, Bath BA2 7AY, UK 3Norwegian Institute for Nature Research, Tungasletta 2, N-7004 Trondheim. Norway ABSTRACT: Gyrodactylus salaris Malmberg, 1957 is an important pathogen in Norwegian populations of Atlantic salmon Salmo salar. It can infect a wide range of salmonid host species, but on most the infections are probably ultimately lim~tedby a host response. Generally, on Norwegian salmon stocks, infections grow unchecked until the host dies. On a Baltic salmon stock, originally from the Neva River, a host reaction is mounted, limltlng parasite population growth on those fishes initially susceptible. Among rainbow trouts Oncorhynchus mykiss from the sam.e stock and among full sib anadromous arctic char Salvelinus alpjnus, both naturally resistant and susceptible individuals later mounting a host response can be observed. This is in contrast to an anadromous stock of brown trout Salmo trutta where only innately resistant individuals were found. A general feature of salmonid infections is the considerable variation of susceptibility between individual fish of the same stock, which appears genetic in origin. The parasite seems to be generally unable to reproduce on non-salmonids, and on cyprinids, individual behavioural mechanisms of the parasite may prevent infection. Transmission occurs directly through host contact, and by detached gyrodactylids and also from dead fishes.
    [Show full text]
  • MASARYKOVA UNIVERZITA Živorodá Monogenea Rodu Gyrodactylus
    MASARYKOVA UNIVERZITA PŘÍRODOVĚDECKÁ FAKULTA ÚSTAV BOTANIKY A ZOOLOGIE Živorodá monogenea rodu Gyrodactylus von Nordmann, 1832 vybraných hlaváčovitých ryb Jaderského moře Bakalářská práce Gabriela Sajlerová Vedoucí práce: Mgr. Iva Přikrylová, Ph.D. Brno 2016 Bibliografický záznam Autor: Gabriela Sajlerová Přírodovědecká fakulta, Masarykova univerzita, Ústav botaniky a zoologie Název práce: Živorodá monogenea rodu Gyrodactylus von Nordmann, 1832 vybraných hlaváčovitých ryb Jaderského moře Studijní program: Evoluční a ekologická biologie Studijní obor: Evoluční a ekologická biologie Vedoucí práce: Mgr. Iva Přikrylová, Ph.D. Akademický rok: 2015/2016 Počet stran: 44 Klíčová slova: Gyrodactylus, hlaváčovité ryby, Jaderské moře, morfometrie, příchytný aparát Bibliographic Entry Author: Gabriela Sajlerová Faculty of Science, Masaryk University, Department of Botany and Zoology Title of Thesis: Viviparous monogenean of the genus Gyrodactylus von Nordmann, 1832 of selected gobiid fishes in Adriatic sea Degree programme: Ecological and Evolutionary Biology Field of study: Ecological and Evolutionary Biology Supervisor: Mgr. Iva Přikrylová, Ph.D. Academic Year: 2015/2016 Number of Pages: 44 Keywords: Gyrodactylus,gobiid fishes, Adriatic Sea, morphometry, attachment apparatus Abstrakt Tato bakalářská práce se sestává ze dvou částí, první literárního přehledu souvisejícího s druhou praktickou části práce, jež se zabývá determinací živorodých monogeneí rodu Gyrodactylus z hlaváčovitých ryb nalovených v Jaderském moři. Na začátku teoretické části je
    [Show full text]
  • Infections with Gyrodactylus Spp. (Monogenea)
    Hansen et al. Parasites & Vectors (2016) 9:444 DOI 10.1186/s13071-016-1727-7 RESEARCH Open Access Infections with Gyrodactylus spp. (Monogenea) in Romanian fish farms: Gyrodactylus salaris Malmberg, 1957 extends its range Haakon Hansen1*,Călin-Decebal Cojocaru2 and Tor Atle Mo1 Abstract Background: The salmon parasite Gyrodactylus salaris Malmberg, 1957 has caused high mortalities in many Atlantic salmon, Salmo salar, populations, mainly in Norway. The parasite is also present in several countries across mainland Europe, principally on rainbow trout, Oncorhynchus mykiss, where infections do not seem to result in mortalities. There are still European countries where there are potential salmonid hosts for G. salaris but where the occurrence of G. salaris is unknown, mainly due to lack of investigations and surveillance. Gyrodactylus salaris is frequently present on rainbow trout in low numbers and pose a risk of infection to local salmonid populations if these fish are subsequently translocated to new localities. Methods: Farmed rainbow trout Oncorhynchus mykiss (n = 340), brook trout, Salvelinus fontinalis (n = 186), and brown trout, Salmo trutta (n = 7), and wild brown trout (n = 10) from one river in Romania were sampled in 2008 and examined for the presence of Gyrodactylus spp. Alltogether 187 specimens of Gyrodactylus spp. were recovered from the fish. A subsample of 76 specimens representing the different fish species and localities were subjected to species identification and genetic characterization through sequencing of the ribosomal internal transcribed spacer 2 (ITS2) and mitochondrial cytochrome c oxidase subunit 1 (cox1). Results: Two species of Gyrodactylus were found, G. salaris and G. truttae Gläser, 1974.
    [Show full text]
  • Speciation and Host–Parasite Relationships in the Parasite Genus
    International Journal for Parasitology 33 (2003) 1679–1689 www.parasitology-online.com Speciation and host–parasite relationships in the parasite genus Gyrodactylus (Monogenea, Platyhelminthes) infecting gobies of the genus Pomatoschistus (Gobiidae, Teleostei)q Tine Huyse*, Vanessa Audenaert, Filip A.M. Volckaert Laboratory of Aquatic Ecology, Katholieke Universiteit Leuven, Ch. De Be´riotstraat 32, B-3000 Leuven, Belgium Received 12 May 2003; received in revised form 14 August 2003; accepted 18 August 2003 Abstract Using species-level phylogenies, the speciation mode of Gyrodactylus species infecting a single host genus was evaluated. Eighteen Gyrodactylus species were collected from gobies of the genus Pomatoschistus and sympatric fish species across the distribution range of the hosts. The V4 region of the ssrRNA and the internal transcribed spacers encompassing the 5.8S rRNA gene were sequenced; by including published sequences a total of 30 species representing all subgenera were used in the data analyses. The molecular phylogeny did not support the morphological groupings into subgenera as based on the excretory system, suggesting that the genus needs systematic revisions. Paraphyly of the total Gyrodactylus fauna of the gobies indicates that at least two independent colonisation events were involved, giving rise to two separate groups, belonging to the subgenus Mesonephrotus and Paranephrotus, respectively. The most recent association probably originated from a host switching event from Gyrodactylus arcuatus, which parasitises three-spined stickleback, onto Pomatoschistus gobies. These species are highly host-specific and form a monophyletic group, two possible ‘signatures’ of co-speciation. Host specificity was lower in the second group. The colonising capacity of these species is illustrated by a host jump from gobiids to another fish order (Anguilliformes), supporting the hypothesis of a European origin of Gyrodactylus anguillae and its intercontinental introduction by the eel trade.
    [Show full text]
  • The Mitochondrial Genome of the Egg-Laying Flatworm
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Springer - Publisher Connector Bachmann et al. Parasites & Vectors (2016) 9:285 DOI 10.1186/s13071-016-1586-2 SHORT REPORT Open Access The mitochondrial genome of the egg- laying flatworm Aglaiogyrodactylus forficulatus (Platyhelminthes: Monogenoidea) Lutz Bachmann1*, Bastian Fromm2, Luciana Patella de Azambuja3 and Walter A. Boeger3 Abstract Background: The rather species-poor oviparous gyrodactylids are restricted to South America. It was suggested that they have a basal position within the otherwise viviparous Gyrodactylidae. Accordingly, it was proposed that the species-rich viviparous gyrodactylids diversified and dispersed from there. Methods: The mitochondrial genome of Aglaiogyrodactylus forficulatus was bioinformatically assembled from next-generation illumina MiSeq sequencing reads, annotated, and compared to previously published mitochondrial genomes of other monogenoidean flatworm species. Results: The mitochondrial genome of A. forficulatus consists of 14,371 bp with an average A + T content of 75.12 %. All expected 12 protein coding, 22 tRNA, and 2 rRNA genes were identified. Furthermore, there were two repetitive non-coding regions essentially consisting of 88 bp and 233 bp repeats, respectively. Maximum Likelihood analyses placed the mitochondrial genome of A. forficulatus in a well-supported clade together with the viviparous Gyrodactylidae species. The gene order differs in comparison to that of other monogenoidean species, with rearrangements mainly affecting tRNA genes. In comparison to Paragyrodactylus variegatus, four gene order rearrangements, i. e. three transpositions and one complex tandem-duplication-random-loss event, were detected. Conclusion: Mitochondrial genome sequence analyses support a basal position of the oviparous A. forficulatus within Gyrodactylidae, and a sister group relationship of the oviparous and viviparous forms.
    [Show full text]
  • Co-Phylogeographic Study of the Flatworm Gyrodactylus Gondae And
    Parasitology International 66 (2017) 119–125 Contents lists available at ScienceDirect Parasitology International journal homepage: www.elsevier.com/locate/parint Co-phylogeographic study of the flatworm Gyrodactylus gondae and its goby host Pomatoschistus minutus Tine Huyse a,b, Merel Oeyen a,1, Maarten H.D. Larmuseau a,c, Filip A.M. Volckaert a,d,⁎ a University of Leuven, Laboratory of Biodiversity and Evolutionary Genomics, Ch. de Bériotstraat 32, B-3000 Leuven, Belgium b Royal Museum for Central Africa, Biology Department, Leuvensesteenweg 13, B-1380 Tervuren, Belgium c University of Leuven, Laboratory of Forensic Genetics and Molecular Archaeology, B-3000 Leuven, Belgium d University of Gothenburg, CeMEB, Department of Marine Sciences, Box 463, SE-405 30 Göteborg, Sweden article info abstract Article history: We performed a comparative phylogeographic study on the monogenean flatworm Gyrodactylus gondae Huyse, Received 29 December 2015 Malmberg & Volckaert 2005 (Gyrodactylidae) and its sand goby host Pomatoschistus minutus (Pallas, 1770) Received in revised form 13 December 2016 (Gobiidae). G. gondae is a host-specific parasite with a direct life cycle and a very short generation time. These Accepted 14 December 2016 properties are expected to increase the chance to track the genealogical history of the host with genetic data of Available online 24 December 2016 the parasite (‘magnifying glass principle’). To investigate this hypothesis we screened nine sand goby populations (n = 326) along the Atlantic coasts of Europe for Gyrodactylus specimens. Low parasite prevalence resulted in Keywords: fi Atlantic Ocean partially overlapping host and parasite datasets. Ninety-two G. gondae collected on ve sand goby populations Co-evolution were subsequently sequenced for a 460 bp cytochrome c oxidase subunit II (coxII) fragment, which, in combina- Gobiidae tion with previously published haplotype data for the hosts, allowed for partially overlapping host and parasite Parasite datasets.
    [Show full text]
  • Horizontal Transmission of the Ectoparasite Gyrodactylus
    B6!!DD 6!;2E1<+22< doi: E2E99EEF;2E122< http://folia.paru.cas.cz Research Article Horizontal transmission of the ectoparasite Gyrodactylus arcuatus (Monogenea: Gyrodactylidae) to the next generation of the three-spined stickleback Gasterosteus aculeatus Jaakko LummeE and B; E !#"G= ; !"#L%6 In the parthenogenetic monogeneans of the genus Gyrodactylus HE15;7!'7@' hosts is determined by the relative roles of lateral transmission and clonal propagation. Clonality and limited transmission lead to 7!73!!B773%@%Gasterosteus aculeatus Linnaeus, the local mitochondrial diversity of Gyrodactylus arcuatus7'%E-557!7ƽ3 '%K7!77>! %@%'!7@%7'M!NNNcox1513 77'[B!7!7'7!777 (h) was >ƽ@'[7'!!@Q hR2-;<h = 2-51SBG@9<U7V sticklebacks carried G. arcuatusK7@![7'6@2-;/E29Q/S G@;';51/+22G@;+K77V' as high as in adults (hR2-E<S@7[7'hR25<9QFSTR2<2S' !K7[[!7[!'@!7 host adulthood via continuous transmission. Nesting and polygamy are suggested as factors maintaining the high genetic diversity of 7K7M7G. arcuatus is fundamentally ƽ7G. salaris@!E-+N7Salmo salar Linnaeus. clonal propagation, competition, parasite transition K7!QEM9S7FF*FF;2E13<+322< Some important aspects of the population genetics and ten overcome by having a very large number of random3 ƽ ly spread propagules. The main characteristics of the host 7 3! ! K7 3 which determine the metapopulation structure and the namics among demes on single hosts in many parasite strategy of the parasite are: (i) the carrying capacity of !@>7M !777*=QS7!3 of host generations and randomness in the transition pro3 ity of the host, relative to the turnover rate of the parasite cess.
    [Show full text]
  • Epidemiological Features of Infection and Species Composition
    Bull. Fr. Pêche Piscic. (1999) 355: 305-325 — 305 — GYRODACTYLIDS PARASITIZING SALMONIDS IN BRITTANY AND WESTERN PYRÉNÉES WATER BASINS : EPIDEMIOLOGICAL FEATURES OF INFECTION AND SPECIES COMPOSITION. A. LAUTRAITE (1), G. BLANC* (2), R. THIERY (3), P. DANIEL (1) and M. VIGNEULLE (3). (1) Groupement de Défense Sanitaire Aquacole d'Aquitaine, 1 rue M. David, BP 219, 40004 MONT-DE-MARSAN Cedex, France. (2) Laboratoire d'Aquaculture et Pathologie Aquacole, Ecole Nationale Vétérinaire de Nantes, Atlanpole-La Chantrerie, BP 40706, 44307 NANTES Cedex 03, France. (3) Agence Française de Sécurité Sanitaire des Aliments Brest, Technopôle Brest-lroise, BP 70, PLOUZANE, F-29280, France. * Corresponding author. ABSTRACT In the first part of a national survey of gyrodactylid parasites, with spécial référence to Gyrodactylus salaris, funded by French administrations, two water basins harbouring Atlantic salmon were sampled : Brittany (7 rivers and 3 restocking farms) and the Adour basin in the Western Pyrénées (6 rivers and 1 restocking farm). 535 salmonids were collected and examined for G. salaris and other gyrodactylid species investigations. Identification procédure was performed by morphological examination and molecular analysis. Both methods led to the same conclusions with a high degree of consistency : Gyrodactylus salaris was declared absent from the examined samples and, therefore, can be considered absent from the sampled water basins with a high level of confidence (over 99.4 %). A new Gyrodactylus species was identified first by morphological examination and confirmed by molecular analysis. This new species is named Gyrodactylus teuchis (CUNNINGHAM et al., in prep.). This resuit has been confirmed by an independent study performed in the same time (Dr.
    [Show full text]
  • Platyhelminthes: Gyrodactylidae and Dactylogyridae)
    bioRxiv preprint doi: https://doi.org/10.1101/283788; this version posted March 17, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. 1 The first next-generation sequencing approach to the mitochondrial phylogeny of African 2 monogenean parasites (Platyhelminthes: Gyrodactylidae and Dactylogyridae) 3 Maarten P.M. Vanhove1,2,3,4,5*, Andrew G. Briscoe6, Michiel W.P. Jorissen3,5, D. Tim J. 4 Littlewood6 & Tine Huyse4,5 5 1 Department of Botany and Zoology, Faculty of Science, Masaryk University, Kotlářská 2, CZ- 6 611 37 Brno, Czech Republic 7 2 Zoology Unit, Finnish Museum of Natural History, P.O.Box 17, FI-00014 University of Helsinki, 8 Helsinki, Finland 9 3 Hasselt University, Centre for Environmental Sciences, Research Group Zoology: Biodiversity 10 & Toxicology, Agoralaan Gebouw D, B-3590 Diepenbeek, Belgium 11 4 Laboratory of Biodiversity and Evolutionary Genomics, Department of Biology, University of 12 Leuven, Ch. Deberiotstraat 32, B-3000 Leuven, Belgium 13 5 Biology Department, Royal Museum for Central Africa, Leuvensesteenweg 13, B-3080 14 Tervuren, Belgium 15 6 Department of Life Sciences, Natural History Museum, Cromwell Road, London SW7 5BD, 16 United Kingdom 17 * Corresponding author. E-mail: [email protected] 18 Emails: 19 Andrew G. Briscoe 20 [email protected] 21 Michiel W.P. Jorissen 22 [email protected] 23 D. Tim J. Littlewood 24 [email protected] 1 bioRxiv preprint doi: https://doi.org/10.1101/283788; this version posted March 17, 2018.
    [Show full text]
  • Fine Structure of the Gastrodermis of Two Species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae)’ Delane C
    Fine Structure of the Gastrodermis of Two Species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae)’ Delane C. Kritsky, Denis Bourguet, Richard D. Spall To cite this version: Delane C. Kritsky, Denis Bourguet, Richard D. Spall. Fine Structure of the Gastrodermis of Two Species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae)’. Transactions of the Amer- ican Microscopical Society, JSTOR, 1994, 113, pp.43 - 51. 10.2307/3226578. hal-02914108 HAL Id: hal-02914108 https://hal.inrae.fr/hal-02914108 Submitted on 11 Aug 2020 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Fine Structure of the Gastrodermis of Two Species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae)' DELANE C. KRITSKY,2 DENIS BOURGUET,23 AND RICHARD D. SPALL4 2 College of Health Professions, Idaho State University, Pocatello, Idaho 83209, U.S.A., Institut des Sciences de l'Evolution, Laboratoire Genetique et Environnement, Universite Montpellier II, Place Eugene Bataillon, 34095 Montpellier cedex 5, France, and Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209, U.S.A. Abstract. Transmission electron microscopy was used to determine the organization and fine structure of the gastrodermis of two species of Gyrodactylus. The gastrodermis consists of a syncytial epithelium characterized by large widely spaced nuclei, two types of vesicular structures, mitochondria with tubular cristae, Golgi complexes, and scattered rough endoplasmic reticulum; the luminal surface possesses few widely spaced, branched lamellae projecting into the intestinal lumen.
    [Show full text]
  • Activities for Prevention of Gyrodactylus Salaris Distribution in the Kola Peninsula Rivers
    BRDEM-2019 International applied research conference «Biological Resources Development and Environmental Management» Volume 2020 Conference Paper Activities for Prevention of Gyrodactylus Salaris Distribution in the Kola Peninsula Rivers Natalia Kalinina1 and Petr Kravets2 1Murmansk Regional Station for animal diseases control, Murmansk, Russia 2Murmansk State Technical University, Murmansk, Russia Abstract Monitoring of distribution of monogenean ectoparasite Gyrodactylus salaris, dangerous for wild populations of the Atlantic salmon in northwest Europe, is carried out by Veterinary Services and the profile scientific organizations in different European countries. In Murmansk area, Gyrodactylus lavareti was first found in a trout farm in Tuloma River in 1996. From now on, representatives of genus Gyrodactylus are annually identified in farmed fish in Tuloma River, according to ichthyopatholocic observations of salmonid farms in Murmansk area. Species G. salaris was indicated in 2016 in farmed trout in Tuloma River and in wild salmon smolts in Pak River of the Corresponding Author: Natalia Kalinina Nizhnetulomsky water basin. Throughout a number of years, the experts of Regional [email protected] Veterinary Service and the scientific organizations of Murmansk area discuss necessity of working out of measures to prevent Gyrodactylus salaris introduction in the rivers Received: 24 December 2019 of the Kola Peninsula with wild populations of the Atlantic salmon. Any transport of Accepted: 9 January 2020 smolt and live fish from the freshwater objects of Baltic Sea basin to the water objects Published: 15 January 2020 of the Barents Sea basin sea would become the most significant threat by parasite Publishing services provided by Gyrodactylus salaris distribution and might cause a damage of natural populations of Knowledge E the Atlantic salmon of Kola Peninsula.
    [Show full text]