LIMS2 (NM 001136037) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC229173

LIMS2 (NM_001136037) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: LIMS2 (NM_001136037) Human Tagged ORF Clone Tag: Myc-DDK Symbol: LIMS2 Synonyms: LGMD2W; MDRCMTT; PINCH-2; PINCH2 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC229173 representing NM_001136037 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGACGGGAAGGAAAAGAAAGTGGGGAGAGACAGGAACAGGGTCCGGAGCAGCCCCAGCAGCAGCATTGA GGTGGAGCAATATGTCGGACGCCTTGGCCAACGCCGTGTGCCAGCGCTGCCAGGCCCGCTTCTCCCCCGC CGAGCGCATTGTCAACAGCAATGGGGAGCTGTACCATGAGCACTGCTTCGTGTGTGCCCAGTGCTTCCGG CCCTTCCCCGAGGGGCTCTTCTATGAGTTTGAAGGCCGGAAGTACTGCGAACACGACTTCCAAATGCTGT TTGCTCCGTGCTGTGGATCCTGCGGTGAGTTCATCATTGGCCGCGTCATCAAGGCCATGAACAACAACTG GCACCCGGGCTGCTTCCGCTGCGAGCTGTGTGATGTGGAGCTGGCTGACCTGGGCTTTGTGAAGAATGCC GGCAGGCATCTCTGCCGGCCTTGCCACAACCGTGAGAAGGCCAAGGGCCTGGGCAAGTACATCTGCCAGC GGTGCCACCTGGTCATCGACGAGCAGCCCCTCATGTTCAGGAGCGACGCCTACCACCCTGACCACTTCAA CTGCACCCACTGTGGGAAGGAGCTGACAGCCGAGGCCCGCGAGCTGAAGGGTGAGCTCTACTGCCTGCCC TGCCATGACAAGATGGGCGTCCCCATCTGCGGGGCCTGCCGCCGGCCCATCGAGGGCCGAGTGGTCAACG CGCTGGGCAAGCAGTGGCACGTGGAGCACTTTGTCTGTGCCAAGTGTGAGAAGCCATTCCTGGGGCACCG GCACTATGAGAAGAAGGGCCTGGCCTACTGCGAGACTCACTACAACCAGCTCTTCGGGGACGTCTGCTAC AACTGCAGCCATGTGATTGAAGGCGATGTGGTGTCGGCCCTCAACAAGGCCTGGTGTGTGAGCTGCTTCT CCTGCTCCACCTGCAACAGCAAGCTCACCCTGAAGAACAAGTTTGTGGAGTTCGACATGAAGCCCGTGTG TAAGAGGTGCTACGAGAAGTTCCCGCTGGAGCTGAAGAAGCGGCTGAAGAAGCTGTCGGAGCTGACCTCC CGCAAGGCCCAGCCCAAGGCCACAGACCTCAACTCTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 3 LIMS2 (NM_001136037) Human Tagged ORF Clone – RC229173

Protein Sequence: >RC229173 representing NM_001136037 Red=Cloning site Green=Tags(s)

MTGRKRKWGETGTGSGAAPAAALRWSNMSDALANAVCQRCQARFSPAERIVNSNGELYHEHCFVCAQCFR PFPEGLFYEFEGRKYCEHDFQMLFAPCCGSCGEFIIGRVIKAMNNNWHPGCFRCELCDVELADLGFVKNA GRHLCRPCHNREKAKGLGKYICQRCHLVIDEQPLMFRSDAYHPDHFNCTHCGKELTAEARELKGELYCLP CHDKMGVPICGACRRPIEGRVVNALGKQWHVEHFVCAKCEKPFLGHRHYEKKGLAYCETHYNQLFGDVCY NCSHVIEGDVVSALNKAWCVSCFSCSTCNSKLTLKNKFVEFDMKPVCKRCYEKFPLELKKRLKKLSELTS RKAQPKATDLNSA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV

Chromatograms: https://cdn.origene.com/chromatograms/mk8054_h10.zip Restriction Sites: SgfI-MluI Cloning Scheme:

Plasmid Map:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 3 LIMS2 (NM_001136037) Human Tagged ORF Clone – RC229173

ACCN: NM_001136037 ORF Size: 1089 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_001136037.3 RefSeq ORF: 1092 bp Locus ID: 55679 UniProt ID: Q7Z4I7, B3KNZ3 MW: 41 kDa Gene Summary: This gene encodes a member of a small family of focal adhesion proteins which interacts with ILK (integrin-linked kinase), a protein which effects protein-protein interactions with the extraceullar matrix. The encoded protein has five LIM domains, each domain forming two zinc fingers, which permit interactions which regulate cell shape and migration. A pseudogene of this gene is located on chromosome 4. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Product images:

Western blot validation of overexpression lysate (Cat# [LY432197]) using anti-DDK antibody (Cat# [TA50011-100]). Left: Cell lysates from un- transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC229173 using transfection reagent MegaTran 2.0 (Cat# [TT210002]).