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(12) United States Patent (10) Patent No.: US 7,608,689 B2 Harris Et Al

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(12) United States Patent (10) Patent No.: US 7,608,689 B2 Harris Et Al USOO7608689B2 (12) United States Patent (10) Patent No.: US 7,608,689 B2 Harris et al. (45) Date of Patent: Oct. 27, 2009 (54) METHODS FOR ENHANCING THE (52) U.S. Cl. ....................... 530/350; 435/200,536/23.2 DEGRADATION OR CONVERSION OF (58) Field of Classification Search ................. 435/200, CELLULOSC MATERIAL 435/41; 536/23.2; 530/350 See application file for complete search history. (75) Inventors: Paul Harris, Carnation, WA (US); Michael Rey, Davis, CA (US); Hanshu (56) References Cited Ding, Davis, CA (US) U.S. PATENT DOCUMENTS (73) Assignee: Novozymes, Inc., David, CA (US) 7,271,244 B2 * 9/2007 Dotson et al. ............... 530/350 2003.0113734 A1 6/2003 Dunn-Coleman et al. (*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 FOREIGN PATENT DOCUMENTS U.S.C. 154(b) by 0 days. WO WO 03/052055 A2 6, 2003 (21) Appl. No.: 11/541,099 OTHER PUBLICATIONS Foreman et al. J. Biol. Chem. 278(34): 31988-319997, Aug. 22, (22) Filed: Sep. 29, 2006 2003.* (65) Prior Publication Data * cited by examiner US 2007/OO77630 A1 Apr. 5, 2007 Primary Examiner Tekchand Saidha (74) Attorney, Agent, or Firm Robert L. Starnes Related U.S. Application Data (60) Provisional application No. 60/722,579, filed on Sep. (57) ABSTRACT 30, 2005. The present invention relates to methods for degrading or (51) Int. Cl. converting a cellulosic material and for producing a Substance C07K L/00 (2006.01) from a cellulosic material. CI2N 9/24 (2006.01) C7H 2L/04 (2006.01) 13 Claims, 5 Drawing Sheets U.S. Patent Oct. 27, 2009 Sheet 2 of 5 US 7,608,689 B2 F1 Origin T7 Primer EcoRI (527) Tree81B 2icron Origin Notl (1721) Reverse Primer Ura N pMB1 Origin Bla A Fig. 2 U.S. Patent Oct. 27, 2009 Sheet 3 of 5 US 7,608,689 B2 EcoRI (768) NA2-tpiPromoter blaA Ncol (1447) Tree81B Ncol (1939) AMG Terminator Pyrg Pyrg Fig. 3 U.S. Patent Oct. 27, 2009 Sheet 4 of 5 US 7,608,689 B2 100% 90% SS 5 80% () h CD 2 70% O (9 8 60% O 2 3 50% -O-GH61B(0.625)+CLF(2.5) -h-cLF(2.5) 40% - - CLF(3.125) 30% O 20 40 60 80 100 120 140 Time, h Fig. 4 U.S. Patent Oct. 27, 2009 Sheet 5 of 5 US 7,608,689 B2 HCLF+Tr61B 9 CLF +Jal250 | 80% 70% 2 2.5 3 3.5 4 Enzyme loading, mg/g PCS US 7,608,689 B2 1. 2 METHODS FOR ENHANCING THE enhancing activity and wherein the polypeptide having cel DEGRADATION OR CONVERSION OF lulolytic enhancing activity is selected from the group con CELLULOSIC MATERIAL sisting of: (a) a polypeptide comprising ILMV-P-x(4,5)-G-X-Y- CROSS-REFERENCE TO RELATED IILMV-X-R-x-EQ-x(3)-A-HNQ, wherein X is any amino APPLICATION acid, X(4.5) is any amino acid at 4 or 5 contiguous positions, and X(3) is any amino acid at 3 contiguous positions; This application claims the benefit of U.S. Provisional (b) a polypeptide comprising an amino acid sequence Application No. 60/722,529, filed Sep. 30, 2005, which appli which has at least 70% identity with the mature polypeptide cation is incorporated herein by reference. 10 of SEQID NO: 2; (c) a polypeptide encoded by a polynucleotide which STATEMENT AS TO RIGHTS TO INVENTIONS hybridizes under at least medium stringency conditions with MADE UNDER FEDERALLY SPONSORED (i) the mature polypeptide coding sequence of SEQID NO: 1. RESEARCH AND DEVELOPMENT (ii) the genomic DNA sequence comprising the mature 15 polypeptide coding sequence of SEQ ID NO: 1, or (iii) a This invention was made with Government support under complementary strand of (i) or (ii); and NREL Subcontract No. ZCO-300 17-02, Prime Contract (d) a variant comprising a conservative Substitution, dele DE-AC36-98GO 10337 awarded by the Department of tion, and/or insertion of one or more amino acids of the Energy. The government has certain rights in this invention. mature polypeptide of SEQID NO: 2. The present invention also relates to methods for producing BACKGROUND OF THE INVENTION a Substance, comprising: (A) saccharifying a cellulosic material with an effective 1. Field of the Invention amount of one or more cellulolytic proteins in the pres The present invention relates to methods for degrading or ence of an effective amount of a polypeptide having converting a cellulosic material and for producing a Substance 25 cellulolytic enhancing activity, wherein the presence of from a cellulosic material. the polypeptide having cellulolytic enhancing activity 2. Description of the Related Art increases the degradation of cellulosic material com Cellulose is a polymer of the simple Sugar glucose pared to the absence of the polypeptide having cellu covalently bonded by beta-1,4-linkages. Many microorgan lolytic enhancing activity and wherein the polypeptide isms produce enzymes that hydrolyze beta-linked glucans. 30 having cellulolytic enhancing activity is selected from These enzymes include endoglucanases, cellobiohydrolases, the group consisting of: and beta-glucosidases. Endoglucanases digest the cellulose (i) a polypeptide comprising IILMV-P-x(4,5)-G-X-Y- polymer at random locations, opening it to attack by cello biohydrolases. Cellobiohydrolases sequentially release mol IILMV-X-R-x-EQ-x(3)-A-HNQ, wherein X is 35 any amino acid, X(4.5) is any amino acid at 4 or 5 ecules of cellobiose from the ends of the cellulose polymer. contiguous positions, and X(3) is any amino acid at 3 Cellobiose is a water-soluble beta-1,4-linked dimer of glu contiguous positions; and cose. Beta-glucosidases hydrolyze cellobiose to glucose. (ii) a polypeptide comprising an amino acid sequence The conversion of cellulosic feedstocks into ethanol has which has at least 70% identity with the mature the advantages of the ready availability of large amounts of 40 polypeptide of SEQID NO: 2: feedstock, the desirability of avoiding burning or land filling (iii) a polypeptide encoded by a polynucleotide which the materials, and the cleanliness of the ethanol fuel. Wood, hybridizes under at least medium stringency condi agricultural residues, herbaceous crops, and municipal Solid tions with (a) the mature polypeptide coding sequence wastes have been considered as feedstocks for ethanol pro of SEQ ID NO: 1, (b) the genomic DNA sequence duction. These materials primarily consist of cellulose, hemi 45 comprising the mature polypeptide coding sequence cellulose, and lignin. Once the cellulose is converted to glu of SEQID NO: 1, or (c) a complementary strand of (i) cose, the glucose is easily fermented by yeast into ethanol. or (ii); and It would be advantageous in the art to improve the ability to (iv) a variant comprising a conservative Substitution, convert cellulosic feedstocks. deletion, and/or insertion of one or more amino acids U.S. Published Patent Application Ser. No. 2003/01 13734 50 of the mature polypeptide of SEQID NO: 2: discloses an isolated cellulase protein, identified as EGVII, and nucleic acids which encode EGVII. (B) fermenting the saccharified cellulosic material of step It is an object of the present invention to provide isolated (a) with one or more fermenting microorganisms; and polypeptides having cellulolytic enhancing activity and iso (C) recovering the substance from the fermentation. lated nucleic acid sequences encoding the polypeptides to 55 In a preferred aspect, the mature polypeptide is amino acids improve the conversion of cellulosic feedstocks. 20 to 249 of SEQID NO: 2. In another preferred aspect, the mature polypeptide coding sequence is nucleotides 77 to 766 SUMMARY OF THE INVENTION of SEQID NO: 1. The present invention further relates to detergent compo The present invention relates to methods for degrading or 60 sitions comprising such polypeptides having cellulolytic converting a cellulosic material, comprising: treating the cel enhancing activity. lulosic material with an effective amount of one or more cellulolytic proteins in the presence of an effective amount of BRIEF DESCRIPTION OF THE FIGURES a polypeptide having cellulolytic enhancing activity, wherein the presence of the polypeptide having cellulolytic enhancing 65 FIG. 1 shows the cDNA sequence and the deduced amino activity increases the degradation of cellulosic material com acid sequence of a Trichoderma reesei RutC30 (ATCC pared to the absence of the polypeptide having cellulolytic 56765) GH61B polypeptide having cellulolytic enhancing US 7,608,689 B2 3 4 activity (SEQ ID NOs: 1 and 2, respectively). Predicted Pre-treated corn Stover: The term 'PCS or “Pre-treated introns are italicized. The predicted signal peptide is under Corn Stover is defined hereinas a cellulosic material derived lined. from corn stover by treatment with heat and dilute acid. For FIG. 2 shows a restriction map of pTr3337. purposes of the present invention, PCS is made by the method FIG.3 shows a restriction map of pTró1B. described in Example 1, or variations thereof in time, tem FIG. 4 shows the hydrolysis of PCS (P020502CS, 100 MF perature and amount of acid. autoclaved, 10 g/L) by Trichoderma reesei broth expressing Family 61 glycoside hydrolase: The term “Family 61 gly Aspergillus Oryzae beta-glucosidase (CLF) with or without coside hydrolase' or “Family GH61 is defined herein as a supplementation by Trichoderma reesei GH61B. Hydrolysis polypeptide falling into the glycoside hydrolase Family 61 was carried out at 50° C., pH 5.0 for the indicated times. 10 according to Henrissat B., 1991. A classification of glycosyl Cellucilast Plus loading was 2.5 or 3.125 mg per gram of PCS hydrolases based on amino-acid sequence similarities, Bio and the mixture contained 2.5 mg of CLF and 0.625 mg of chem.
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