O101-S Examination for FOB in the Common Marmoset 1P001-T
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O101-S Examination for FOB in the common marmoset 1 2 2 2 ○Inoue Ryo ,Atsushi FUJIWARA ,Kazue ARUGA ,Masahiko IINO ,Chiyoko NISHIME1,Eiko NISHINAKA1,Shinichi SATO2 1Central Institute for Experimental Animals 2Ina Research Inc Purpose:The common marmoset (marmoset) is small primates, which their body weights are about the same as rats. Functional Observational Battery (FOB) is a test to evaluate the effect of a compound on the central nervous systems (CNS) in drug development. In this study, we investigated if the marmoset is useful to possess high-sensitivity primate-specific FOB.Methods:Saline, Cocaine (Coc 2 or 1 mg/kg) or Chlorpromazine (Cpz 3 or 1 mg/kg) was intravenously administered to four male marmosets. FOB (general condition, motor function, etc.) was performed 5 minutes to 6 hours after the administration.Results:Rapid and Slight movements of the head which is sepcific for the non-human primate, continual movement, hyperirritability and hyperthermia were observed at 5 min after administration in Coc 2 mg/kg group. Although continual movement, hyperirritability and hyperthermia were also observed in Coc 1 mg/kg group, expression of other symptoms were decreased. Eye-closing, hypoactivity, slowed motion and ataxia were observed at 5 min after administration of Cpz 3 mg/kg. Eye-closing, hypoactivity were also observed in Cpz 1 mg/kg group, but expression of other symptoms were decreased.Discussion:Dose dependent effects of Coc and Cpz on the CNS were able to be detected in the marmoset FOB. Since the primate-specific symptom such as rapid and slight of the head movement was observed in the Coc treatment, FOB in the marmosets may be more useful than that in rats. 1P001-T Introduction of Institute of Laboratory Animal Research, Nagoya University ○Mariko Itoh,Rarami Mori,Mayumi Toda,SHuichi Aono,Jun Sato Center for Animal Research and Education, Nagoya University, Nagoya, Japan In Higashiyama area of Nagoya University, Institute of Laboratory Animal Facility, a shared new SPF (specific pathogen free) facility, has opened. It is a three-story facility with total floor area of 2,218 m2 for rats and mice. By this facility, we efficiently centralized the management of animals in Higashiyama area, and established a safer and more appropriate environment for animal experimentation. S 87 1P002-T The large-scale repair and renovation work conducted in animal facility of Niigata University (3) 1 1 1 1 ○Yoshitaka MAEDA , Kanako ODA , Seiko SAKAI , Fumiya NAGATA , Yukihiro JIMBO1, Minoru TANAKA1, Yoshitaka YAMAMOTO1, Saori CHIBA1, Toshiya SATO1, Nobuyoshi FUJISAWA1, Minesuke YOKOYAMA1,2, Toshikuni SASAOKA1 1) Animal Resources Branch Department of Comparative & Experimental Medicine Brain Research Institute, Niigata University, Niigata, Japan 2) Central Institute for Experimental Animals, Kawasaki, Japan The animal facility of Brain Research Institute, Niigata University conducted a major building renovation over a period of seven months from January 2013. Last year in the Annual Meeting of the Japanese Association for Experimental Animal Technologists, we reported two papers entitled "Shutdown and transfer of the animal facility" and "Management of temporary facility and re-launch of the new facility." In the present paper, we will report the experience in the process of resuming our facility. 1P003-T Aseismic measures in the new laboratory animal facility ○Satomi Takano, Kaori Muguruma, Noboru Ogiso Laboratory of Research Animal, National Center for Geriatrics and Gerontology, Aichi, Japan Earthquake measures have been implemented in research institutions and universities from the lessons of the Great East Japan Earthquake and the Great Hanshin Earthquake. In the new laboratory animal facility which was completed in September 2012, we try to carry out seismic measures in NCGG with experts. Most of aseismic fixing method was need to drill a hole in the floor or walls so far. But this method is not necessarily suitable to the laboratory and breeding room because cleaning and disinfection of the day-to-day is difficult. Recently, the seismic fixing bracket which combines earthquake-resistant mat and metal parts was developed. This fixing bracket does not need holes in the floor or walls. In this study, we examined whether or not this fixing bracket is useful in laboratory and breeding room. We were attached the seismic fixing bracket to individual ventilated breeding rack and gas cylinder mount. We were compared with before mounting for deterioration of the mat cleanliness and workability and, of seismic safety equipment for the installation location. The cylinder frame, there was no effect on the vibration rather than hinder the work of the cylinder desorption. We are currently studying the cleanliness measurement of seismic equipment and work due to desorption of the fixing bracket. S 88 1P004-T Efforts of Laboratory Animals Managers in AAALAC International accreditation ○Kazutaka Fujita, Kosaka Mitsuhiro, Yoshio Nemoto, Yukari Nishio, Kinya Fujii, Tsuyoshi Mizoguchi, Muneo Haraguchi, Shizuka Nitta, Masanori Kanda Shionogi TechnoAdvance Research Co., Ltd In receiving AAALAC International accreditation, our laboratory animal manager has been largely involved in three major points as follows: identification of issues and revision of operating procedures, introduction of veterinary care, and environmental enrichment. Through these activities, we reaffirmed important roles of the laboratory animal manager, and animal observation and facility management were centered on the animal. Future subjects will be also discussed. 1P005-T Construction of rearing management system with bar-code ○Chika Uefuji, Norio Yata, Kazutaka Ueyama, Masayuki Arakawa, Masahiro Fujii, Haruko Hirayama, Katsumi Mominoki Department of Animal Resources, Advanced Science Research Center, Okayama University, Okayama, Japan To efficiently manage the animal facility, it is important that the quantity of the laboratory animals, especially transgenic mice, have been a handle on. However, there are too many difficulties, time-consuming, in doing this work without a support system for the management of the animals. Although there have been commercially available ones, these initial installations are more expensive. Moreover, these systems are less flexible under saving the cost. Therefore, we have been trying to come up with the low-cost and flexible system using a general-purpose component such as King Jim Tepra Pro, and a widely used software with the visual basic for application (VBA), such as Microsoft Excel. If the computer network has already been established in the facility, the initial cost is less than 150,000 Yen per one package, includes a tablet type personal computer with the required software, a bar cord reader and a label printer. Namely, VBA allows our developed system to easily, cheaply and unlimitedly expand the specifications in proportion to the increase in the volume of information. The high flexibility of our system has been helpful to easily get a handle on the quantity of the animals in our facility, so that we propel the concept of three Rs, especially "Reduction" , in our facility. S 89 1P006-S Development of Web-Based Laboratory Animal Care System 1 1 1 1 1 ○Jun Sato ,Shuichi Aono ,Akiko Tomotsugu ,Mariko Itoh ,Rarami Mori ,Mayumi Toda1,Nobuya Shosaki2 1Center for Animal Research and Education, Nagoya University, Nagoya, Japan 2EVANEXT limited partnership company, Kakogawa, Hyogo, Japan There are many rearing management items in animal experiments, and in order to support the smooth operation of the laboratory animal facility, it is necessary to develop an optimal management system. However, since the existing systems are expensive, it does not promote the introduction of a software system at universities or research institutes. Although the introduction of the paper-based system is a cost-cutter, it takes up a lot of time and work to convert paper-based information to digital format. Moreover, it is not desirable for hygienic environment. We developed a web-based laboratory animal care system and have been innovating the developed system since July 2013. Users can feed information to diary management and husbandry from a tablet computer at the animal rooms, and send animal's information in real time to a Web server. The management states and the animal experimental plan are accessible from a computer with permission connected to the Internet. By storing in the database automatically, we reduce the time and effort spent to tallying process. Furthermore, fees and billing are automatically calculated based on the information obtained from the diary management and husbandry. As a future subject, we would like to provide guidance about information moral and strengthen information security. 1P007-T Significance of microbial monitoring in the animal facility environment: Part 2 1 2 2 ○Yuko Moriya ,Syuki Ojima , Ryuichiro Ando 1JAC, Inc., Tokyo, Japan 2 Center for Laboratory Animal Science, Tohoku Pharmaceutical University, Sendai, Japan In order to grasp if the facility environment is properly maintained, we performed setting plate sampling before and after cleaning and disinfecting the floor. Results of the comparative analysis on an annual basis, the number of bacteria was reduced after disinfection and a significant difference was observed in 10 of 23 areas. In order to maintain proper environment, the time and frequency of the cleaning and disinfection should be considered. S 90 1P008-S Evaluation experiment of indoor decontamination using