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Pinpointing the Origin of Mitochondria Zhang Wang Hanchuan, Hubei
Pinpointing the origin of mitochondria Zhang Wang Hanchuan, Hubei, China B.S., Wuhan University, 2009 A Dissertation presented to the Graduate Faculty of the University of Virginia in Candidacy for the Degree of Doctor of Philosophy Department of Biology University of Virginia August, 2014 ii Abstract The explosive growth of genomic data presents both opportunities and challenges for the study of evolutionary biology, ecology and diversity. Genome-scale phylogenetic analysis (known as phylogenomics) has demonstrated its power in resolving the evolutionary tree of life and deciphering various fascinating questions regarding the origin and evolution of earth’s contemporary organisms. One of the most fundamental events in the earth’s history of life regards the origin of mitochondria. Overwhelming evidence supports the endosymbiotic theory that mitochondria originated once from a free-living α-proteobacterium that was engulfed by its host probably 2 billion years ago. However, its exact position in the tree of life remains highly debated. In particular, systematic errors including sparse taxonomic sampling, high evolutionary rate and sequence composition bias have long plagued the mitochondrial phylogenetics. This dissertation employs an integrated phylogenomic approach toward pinpointing the origin of mitochondria. By strategically sequencing 18 phylogenetically novel α-proteobacterial genomes, using a set of “well-behaved” phylogenetic markers with lower evolutionary rates and less composition bias, and applying more realistic phylogenetic models that better account for the systematic errors, the presented phylogenomic study for the first time placed the mitochondria unequivocally within the Rickettsiales order of α- proteobacteria, as a sister clade to the Rickettsiaceae and Anaplasmataceae families, all subtended by the Holosporaceae family. -
Rhizoplane Enrichment Process of the Citrus Root-Associated Microbiome Yunzeng Zhang1†, Jin Xu1†, Nadia Riera1†, Tao Jin2, Jinyun Li1 and Nian Wang1,3*
Zhang et al. Microbiome (2017) 5:97 DOI 10.1186/s40168-017-0304-4 RESEARCH Open Access Huanglongbing impairs the rhizosphere-to- rhizoplane enrichment process of the citrus root-associated microbiome Yunzeng Zhang1†, Jin Xu1†, Nadia Riera1†, Tao Jin2, Jinyun Li1 and Nian Wang1,3* Abstract Background: Roots are the primary site for plant-microbe interactions. Among the three root-associated layers (i.e., rhizosphere, rhizoplane, and endorhiza), the rhizoplane is a key component serving a critical gating role that controls microbial entry into plant roots. The microbial communities colonizing the three layers are believed to be gradually enriched from the bulk soil inoculum. However, it is unknown how this enrichment process, particularly the rhizosphere to rhizoplane step, is affected by biotic stresses, such as disease. In this study, we address this question using the citrus root-associated microbiome as a model. Results: We identified the rhizosphere-to-rhizoplane-enriched taxonomic and functional properties of the citrus root- associated microbiome and determined how they were affected by Huanglongbing (HLB), a severe systemic disease caused by Candidatus Liberibacter asiaticus, using metagenomic and metatranscriptomic approaches. Multiple rhizoplane-enriched genera were identified, with Bradyrhizobium and Burkholderia being the most dominant. Plant-derived carbon sources are an important driving force for the enrichment process. The enrichment of functional attributes, such as motility, chemotaxis, secretion systems, and lipopolysaccharide -
Metaproteomics Characterization of the Alphaproteobacteria
Avian Pathology ISSN: 0307-9457 (Print) 1465-3338 (Online) Journal homepage: https://www.tandfonline.com/loi/cavp20 Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar To cite this article: José Francisco Lima-Barbero, Sandra Díaz-Sanchez, Olivier Sparagano, Robert D. Finn, José de la Fuente & Margarita Villar (2019) Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssusgallinae (De Geer, 1778), Avian Pathology, 48:sup1, S52-S59, DOI: 10.1080/03079457.2019.1635679 To link to this article: https://doi.org/10.1080/03079457.2019.1635679 © 2019 The Author(s). Published by Informa View supplementary material UK Limited, trading as Taylor & Francis Group Accepted author version posted online: 03 Submit your article to this journal Jul 2019. Published online: 02 Aug 2019. Article views: 694 View related articles View Crossmark data Citing articles: 3 View citing articles Full Terms & Conditions of access and use can be found at https://www.tandfonline.com/action/journalInformation?journalCode=cavp20 AVIAN PATHOLOGY 2019, VOL. 48, NO. S1, S52–S59 https://doi.org/10.1080/03079457.2019.1635679 ORIGINAL ARTICLE Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) José Francisco Lima-Barbero a,b, Sandra Díaz-Sanchez a, Olivier Sparagano c, Robert D. Finn d, José de la Fuente a,e and Margarita Villar a aSaBio. -
Selection of Endophytic Strains for Enhanced Bacteria-Assisted Phytoremediation of Organic Pollutants Posing a Public Health Hazard
International Journal of Molecular Sciences Review Selection of Endophytic Strains for Enhanced Bacteria-Assisted Phytoremediation of Organic Pollutants Posing a Public Health Hazard Magdalena Anna Kara´s*, Sylwia Wdowiak-Wróbel and Wojciech Sokołowski Department of Genetics and Microbiology, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland; [email protected] (S.W.-W.); [email protected] (W.S.) * Correspondence: [email protected]; Tel.: +48-81-537-50-58 Abstract: Anthropogenic activities generate a high quantity of organic pollutants, which have an impact on human health and cause adverse environmental effects. Monitoring of many hazardous contaminations is subject to legal regulations, but some substances such as therapeutic agents, personal care products, hormones, and derivatives of common organic compounds are currently not included in these regulations. Classical methods of removal of organic pollutants involve economically challenging processes. In this regard, remediation with biological agents can be an alternative. For in situ decontamination, the plant-based approach called phytoremediation can be used. However, the main disadvantages of this method are the limited accumulation capacity of plants, sensitivity to the action of high concentrations of hazardous pollutants, and no possibility of using pollutants for growth. To overcome these drawbacks and additionally increase the efficiency of Citation: Kara´s,M.A.; the process, an integrated technology of bacteria-assisted phytoremediation is being used recently. Wdowiak-Wróbel, S.; Sokołowski, W. For the system to work, it is necessary to properly select partners, especially endophytes for specific Selection of Endophytic Strains for Enhanced Bacteria-Assisted plants, based on the knowledge of their metabolic abilities and plant colonization capacity. -
Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical Thalassohaline Environment
RESEARCH ARTICLE Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical Thalassohaline Environment Christophe Leboulanger1*, HeÂlène Agogue 2☯, CeÂcile Bernard3☯, Marc Bouvy1☯, Claire Carre 1³, Maria Cellamare3¤³, Charlotte Duval3³, Eric Fouilland4☯, Patrice Got4☯, Laurent Intertaglia5³, CeÂline Lavergne2³, Emilie Le Floc'h4☯, CeÂcile Roques4³, GeÂrard Sarazin6☯ a1111111111 1 UMR MARBEC, Institut de Recherche pour le DeÂveloppement, Sète-Montpellier, France, 2 UMR LIENSs, Centre National de la Recherche Scientifique, La Rochelle, France, 3 UMR MCAM, MuseÂum National a1111111111 d'Histoire Naturelle, Paris, France, 4 UMR MARBEC, Centre National de la Recherche Scientifique, Sète- a1111111111 Montpellier, France, 5 Observatoire OceÂanologique de Banyuls-sur-Mer, Universite Pierre et Marie Curie, a1111111111 Banyuls-sur-Mer, France, 6 UMR7154 Institut de Physique du Globe de Paris, Universite Paris Diderot, a1111111111 Paris, France ☯ These authors contributed equally to this work. ¤ Current address: Phyto-Quality, 15 rue PeÂtrarque, Paris, France ³ These authors also contributed equally to this work. * [email protected] OPEN ACCESS Citation: Leboulanger C, Agogue H, Bernard C, Bouvy M, Carre C, Cellamare M, et al. (2017) Abstract Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical This study describes, for the first time, the water chemistry and microbial diversity in Dziani Thalassohaline Environment. PLoS ONE 12(1): e0168879. doi:10.1371/journal.pone.0168879 Dzaha, a tropical crater lake located on Mayotte Island (Comoros archipelago, Western Indian Ocean). The lake water had a high level of dissolved matter and high alkalinity (10.6± Editor: Jean-FrancËois Humbert, INRA, FRANCE -1 2- 14.5 g L eq. -
Taxonomic Hierarchy of the Phylum Proteobacteria and Korean Indigenous Novel Proteobacteria Species
Journal of Species Research 8(2):197-214, 2019 Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species Chi Nam Seong1,*, Mi Sun Kim1, Joo Won Kang1 and Hee-Moon Park2 1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea *Correspondent: [email protected] The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju. -
Commensal and Pathogenic Members of the Dental Calculus Microbiome of Badia Pozzeveri Individuals from the 11Th to 19Th Centuries
G C A T T A C G G C A T genes Article Commensal and Pathogenic Members of the Dental Calculus Microbiome of Badia Pozzeveri Individuals from the 11th to 19th Centuries Tasha M. Santiago-Rodriguez 1,*, Antonio Fornaciari 2,*, Gino Fornaciari 3, Stefania Luciani 4, Isolina Marota 4 , Giuseppe Vercellotti 5, Gary A. Toranzos 6, Valentina Giuffra 2 and Raul J. Cano 7 1 Diversigen Inc., Houston, TX 77021, USA 2 Department of Translational Research on New Technologies in Medicine and Surgery, Division of Paleopathology, University of Pisa, 56128 Pisa, Italy; v.giuff[email protected] 3 Department of Civilizations and Forms of Knowledge, University of Pisa, 56128 Pisa, Italy; [email protected] 4 Laboratory of Molecular Archaeo-Anthropology/ancient DNA, School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino, Italy; [email protected] (S.L.); [email protected] (I.M.) 5 Department of Anthropology, The Ohio State University, Columbus, OH 31901, USA; [email protected] 6 Department of Biology, University of Puerto Rico, San Juan, PR 00931, USA; [email protected] 7 The BioCollective, Denver, CO 80014, USA; [email protected] * Correspondence: [email protected] (T.M.S.-R.); [email protected] (A.F.) Received: 1 February 2019; Accepted: 5 April 2019; Published: 12 April 2019 Abstract: The concept of the human oral microbiome was applied to understand health and disease, lifestyles, and dietary habits throughout part of human history. In the present study, we augment the understanding of ancient oral microbiomes by characterizing human dental calculus samples recovered from the ancient Abbey of Badia Pozzeveri (central Italy), with differences in socioeconomic status, time period, burial type, and sex. -
Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater
sustainability Article Ultramicrobacteria from Nitrate- and Radionuclide-Contaminated Groundwater Tamara Nazina 1,2,* , Tamara Babich 1, Nadezhda Kostryukova 1, Diyana Sokolova 1, Ruslan Abdullin 1, Tatyana Tourova 1, Vitaly Kadnikov 3, Andrey Mardanov 3, Nikolai Ravin 3, Denis Grouzdev 3 , Andrey Poltaraus 4, Stepan Kalmykov 5, Alexey Safonov 6, Elena Zakharova 6, Alexander Novikov 2 and Kenji Kato 7 1 Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (T.B.); [email protected] (N.K.); [email protected] (D.S.); [email protected] (R.A.); [email protected] (T.T.) 2 V.I. Vernadsky Institute of Geochemistry and Analytical Chemistry of Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] 3 Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (V.K.); [email protected] (A.M.); [email protected] (N.R.); [email protected] (D.G.) 4 Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] 5 Chemical Faculty, Lomonosov Moscow State University, 119991 Moscow, Russia; [email protected] 6 Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071 Moscow, Russia; [email protected] (A.S.); [email protected] (E.Z.) 7 Faculty of Science, Department of Geosciences, Shizuoka University, 422-8529 Shizuoka, Japan; [email protected] -
Genomic Evidence of the Illumination Response Mechanism and Evolutionary History of Magnetotactic Bacteria Within the Rhodospiri
Wang et al. BMC Genomics (2019) 20:407 https://doi.org/10.1186/s12864-019-5751-9 RESEARCH ARTICLE Open Access Genomic evidence of the illumination response mechanism and evolutionary history of magnetotactic bacteria within the Rhodospirillaceae family Yinzhao Wang1,2,3* , Giorgio Casaburi4, Wei Lin2, Ying Li5, Fengping Wang1 and Yongxin Pan2,3 Abstract Background: Magnetotactic bacteria (MTB) are ubiquitous in natural aquatic environments. MTB can produce intracellular magnetic particles, navigate along geomagnetic field, and respond to light. However, the potential mechanism by which MTB respond to illumination and their evolutionary relationship with photosynthetic bacteria remain elusive. Results: We utilized genomes of the well-sequenced genus Magnetospirillum, including the newly sequenced MTB strain Magnetospirillum sp. XM-1 to perform a comprehensive genomic comparison with phototrophic bacteria within the family Rhodospirillaceae regarding the illumination response mechanism. First, photoreceptor genes were identified in the genomes of both MTB and phototrophic bacteria in the Rhodospirillaceae family, but no photosynthesis genes were found in the MTB genomes. Most of the photoreceptor genes in the MTB genomes from this family encode phytochrome-domain photoreceptors that likely induce red/far-red light phototaxis. Second, illumination also causes damage within the cell, and in Rhodospirillaceae, both MTB and phototrophic bacteria possess complex but similar sets of response and repair genes, such as oxidative stress response, -
Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical Thalassohaline Environment
RESEARCH ARTICLE Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical Thalassohaline Environment Christophe Leboulanger1*, HeÂlène Agogue 2☯, CeÂcile Bernard3☯, Marc Bouvy1☯, Claire Carre 1³, Maria Cellamare3¤³, Charlotte Duval3³, Eric Fouilland4☯, Patrice Got4☯, Laurent Intertaglia5³, CeÂline Lavergne2³, Emilie Le Floc'h4☯, CeÂcile Roques4³, GeÂrard Sarazin6☯ a1111111111 1 UMR MARBEC, Institut de Recherche pour le DeÂveloppement, Sète-Montpellier, France, 2 UMR LIENSs, Centre National de la Recherche Scientifique, La Rochelle, France, 3 UMR MCAM, MuseÂum National a1111111111 d'Histoire Naturelle, Paris, France, 4 UMR MARBEC, Centre National de la Recherche Scientifique, Sète- a1111111111 Montpellier, France, 5 Observatoire OceÂanologique de Banyuls-sur-Mer, Universite Pierre et Marie Curie, a1111111111 Banyuls-sur-Mer, France, 6 UMR7154 Institut de Physique du Globe de Paris, Universite Paris Diderot, a1111111111 Paris, France ☯ These authors contributed equally to this work. ¤ Current address: Phyto-Quality, 15 rue PeÂtrarque, Paris, France ³ These authors also contributed equally to this work. * [email protected] OPEN ACCESS Citation: Leboulanger C, Agogue H, Bernard C, Bouvy M, Carre C, Cellamare M, et al. (2017) Abstract Microbial Diversity and Cyanobacterial Production in Dziani Dzaha Crater Lake, a Unique Tropical This study describes, for the first time, the water chemistry and microbial diversity in Dziani Thalassohaline Environment. PLoS ONE 12(1): e0168879. doi:10.1371/journal.pone.0168879 Dzaha, a tropical crater lake located on Mayotte Island (Comoros archipelago, Western Indian Ocean). The lake water had a high level of dissolved matter and high alkalinity (10.6± Editor: Jean-FrancËois Humbert, INRA, FRANCE -1 2- 14.5 g L eq. -
Identification of Pseudomonas Species and Other Non-Glucose Fermenters
UK Standards for Microbiology Investigations Identification of Pseudomonas species and other Non- Glucose Fermenters Issued by the Standards Unit, Microbiology Services, PHE Bacteriology – Identification | ID 17 | Issue no: 3 | Issue date: 13.04.15 | Page: 1 of 41 © Crown copyright 2015 Identification of Pseudomonas species and other Non-Glucose Fermenters Acknowledgments UK Standards for Microbiology Investigations (SMIs) are developed under the auspices of Public Health England (PHE) working in partnership with the National Health Service (NHS), Public Health Wales and with the professional organisations whose logos are displayed below and listed on the website https://www.gov.uk/uk- standards-for-microbiology-investigations-smi-quality-and-consistency-in-clinical- laboratories. SMIs are developed, reviewed and revised by various working groups which are overseen by a steering committee (see https://www.gov.uk/government/groups/standards-for-microbiology-investigations- steering-committee). The contributions of many individuals in clinical, specialist and reference laboratories who have provided information and comments during the development of this document are acknowledged. We are grateful to the Medical Editors for editing the medical content. For further information please contact us at: Standards Unit Microbiology Services Public Health England 61 Colindale Avenue London NW9 5EQ E-mail: [email protected] Website: https://www.gov.uk/uk-standards-for-microbiology-investigations-smi-quality- and-consistency-in-clinical-laboratories -
Carcinogenic Pesticide Control Via Hijacking Endosymbiosis; The
in vivo 32 : 1051-1062 (2018) doi:10.21873/invivo.11346 Carcinogenic Pesticide Control via Hijacking Endosymbiosis; The Paradigm of DSB-A from Wolbachia pipientis for the Management of Otiorhynchus singularis THOMAS KOSTAROPOULOS 1, LOUIS PAPAGEORGIOU 1, SPYRIDON CHAMPERIS TSANIRAS 2, DIMITRIOS VLACHAKIS 1,3 and ELIAS ELIOPOULOS 1 1Genetics Laboratory, Department of Biotechnology, Agricultural University of Athens, Athens, Greece; 2Department of Physiology, School of Medicine, University of Patras, Patras, Greece; 3Faculty of Natural & Mathematical Sciences, King's College London, London, U.K. Abstract. Background/Aim: Pesticides have little, if any Nowadays cancer has evolved to a worldwide plague; specificity, to the pathogen they target in most cases. Wide according to the latest statistics cancer is at the top of the list spectrum toxic chemicals are being used to remove pestcides of causes of death worldwide (1, 2). It is a multi-step process and salvage crops and economies linked to agriculture. The (3) associated with genomic instability (4, 5) and caused by a burden on the environment, public health and economy is variety of factors and substances, called carcinogens, including huge. Traditional pestcide control is based on administering radiation, tobacco and alcohol, as well as lifestyle factors heavy loads of highly toxic compounds and elements that including an unhealthy diet, obesity and physical inaction essentially strip all life from the field. Those chemicals are (6-9). Likewise, chemicals of pesticides have been associated a leading cause of increased cancer related deaths in with increased incidence of cancer to those who are exposed countryside. Herein, the Trojan horse of endosymbiosis was to them directly or implicitly through their diet or their used, in an effort to control pests using high specificity environment (10).