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Environmentasia EnvironmentAsia AvailableAvailable online online at www.tshe.org/EA at www.tshe.org/EA The international journal published by the Thai Society of Higher Education Institutes on Environment EnvironmentAsiaEnvironmentAsia 28(1) (2009) (2015) 50-54 41-52 GenotoxicityUsing SWAT Assessment to Assess the of CriticalMercuric Areas Chloride and Nonpoint in the Marine Source Fish Pollution Therapon Reduction jaruba Best Management Practices in Lam Takong River Basin, Thailand Nagarajan Nagarani, Arumugam Kuppusamy Kumaraguru, Velmurugan Janaki Devi Netnapa Pongpetch a, Pongthepand ChandrasekaranSuwanwaree a, Chatpet Archana Yossapol Devi b, Songkot Dasananda c and Thongplew Kongjun d Center for Marine and Coastal Studies, School of Energy, Environment and Natural Resources, a School of Biology, InstituteMadurai of Science, Kamaraj Suranaree University, University Madurai-625021, of Technology, NakhonIndia Ratchasima, Thailand b School of Environmental Engineering, Institute of Engineering, Suranaree University of Technology, Nakhon Ratchasima, Thailand Abstractc School of Remote Sensing, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, Thailand d Office of Water Management and Hydrology, Royal Irrigation Department, Ministry of Agriculture and Cooperatives, The aim of the present study was to standardize andThailand to assess the predictive value of the cytogenetic analysis by Micronucleus (MN) test in fish erythrocytes as a biomarker for marine environmental contamination. Micronucleus frequencyAbstract baseline in erythrocytes was evaluated in and genotoxic potential of a common chemical was determined in fish experimentally exposed in aquarium under controlled conditions. Fish (Therapon jaruba) were exposed for 96 hrs toHydrological a single heavy models metal are (mercuric essential chloride). tools for waterChromosomal resource damageand nonpoint was determined source pollution as micronuclei management. frequency This study in fishaimed erythrocytes. to evaluate critical Significant areas andincrease best management in MN frequency practices was (BMPs) observed of sediment in erythrocytes and nutrient of fish loads exposed in Lam toTakong mercuric River chloride.basin, Northeastern Concentration Thailand, of 0.25 using ppm SWAT induced (Soil the and highest Water MN Assessment frequency Tool) (2.95 model. micronucleated The model wascells/1000 calibrated cells and compared validated tousing 1 MNcell/1000 daily data of streamflow,cells in control sediment, animals). NO3-N The and study TP in revealed Lam Takong that micronucleusRiver from 2007-2008 test, as anand index 2009, of respectively. cumulative In exposure,general, the appears simulated to bestreamflow a sensitive and model sediment to evaluate were in reasonablegenotoxic compoundsagreement with in fishthe undermeasured controlled values with conditions. coefficient of determination (R2) and Nash-Sutcliffe model efficiency coefficient (NSE) greater than 0.50 and the percent bias (PBIAS) Keywords:less than 25%. genotoxicity; Additionally, mercuric nutrient chloride; loads showed micronucleus a fair relationship between observation and simulation with R2 values more than 0.6 and PBIAS values less than 25%. From simulation, September was the month with the highest sediment, NO3-N and TP yields while January and December were the lowest months. From the model, SWAT identified 1 severe and 1 high soil erosion subbasins. Two subbasins were classified into medium loading for NO3-N. However, 9 subbasins were 1.classified Introduction into high loading rate of TP. For BMPs, the 30-m laboratorywide filter strip and fieldwas the conditions. best scenario In reducing2006 Soumendra 100% of both sediment and TP, and 97.27% of NO3-N. These results could etbe al a .,useful made tool an forattempt water to resources detect genetic managements biomarkers and soil conservationIn India, planning about in200 Lam tons Takong of Rivermercury basin. and its in two fish species, Labeo bata and Oreochromis compounds are introduced into the environment mossambica, by MN and binucleate (BN) annuallyKeywords: as hydrological effluents frommodel; industries nitrate; phosphorus; (Saffi, 1981). soil erosion;erythrocytes watershed in the gill and kidney erythrocytes Mercuric chloride has been used in agriculture as a exposed to thermal power plant discharge at fungicide, in medicine as a topical antiseptic and Titagarh Thermal Power Plant, Kolkata, India. disinfectant,1. Introduction and in chemistry as an intermediate in districtsThe presentof three study provinces was conducted including toPracheenburi, determine the production of other mercury compounds. The theNakhon acute Nayokgenotoxicity and Nakhon of the heavy Ratchasima metal compound provinces. Nonpoint source (NPS) pollution of streams, lakes The dominant land use is agriculture land. Many contamination of aquatic ecosystems by heavy HgCl2 in static systems. Mercuric chloride is toxic, metalsand estuaries and pesticides has created has agained critical increasing concern throughout attention solvableorganizations, in water such hence as it the can Hydrology penetrate the and aquatic Water inthe recentworld. Agriculturaldecades. Chronic activities exposurehave been identifiedto and animals.Management Mutagenic Center studies for the with Lower native Northeastern fish species accumulationas the primary sourcesof these of chemicals NPS pollutants. in aquatic In Thailand, biota representRegion, the an Pollutionimportant Control effort inDepartment determining (PCD), the canwater result pollution in tissue is largely burdens associated that produce with urbanization, adverse potentialRegional effectsEnvironmental of toxic Office agents. 11 This and studyMunicipality was effectsindustrialization not only inand the agricultural directly exposed activities. organisms, The main carriedof Nakhon out toRatchasima, evaluate the have use studied of the micronucleushydrology and butpollutants also in humanfor surface beings. water quality problems are testwater (MN) quality for ofthe Lam estimation Takong of River. aquatic Water pollution quality sediments,Fish provides nutrients a suitableand other model chemical for monitoring substances usingdegradation marine hasedible been fish observed under lab for conditions. more than two aquatic(Office ofgenotoxicity Natural Resources and andwastewater Environmental quality Policy decades, as a result of urbanization and intensive becauseand Planning, of its 2012).ability However,to metabolize the governing xenobiotics policies and 2.farming Materials (Pollution and Control methods Department, 2008). Previous accumulatedon watershed managementpollutants. A in micronucleus the past, did not assay appreciate has studies did not evaluate temporal and spatial sediment beenthe importance used successfully of NPS pollution in several (Simachaya, species (De 2003) Flora, and 2.1.and nutrientSample loads Collection at basin level which can sufficiently etfew al strategies., 1993, Al-Sabtiexist for andremediating Metcalfe, the 1995).issue (Babel The reduce NPS pollution. micronucleuset al., 2004; Pollution (MN) Control test has Department, been developed 2006). TheContinuous fish species water selected quality for monitoring the present isstudy very together Lam Takongwith DNA-unwinding River basin is crucial assays to asthe wasexpensive, collected time from consuming Pudhumadam and spatially coast impractical of Gulf of at perspectiveNortheastern regionmethods of Thailand. for mass More monitoring than 880,000 of Mannar,the watershed Southeast level. Therefore, Coast of mathematical India. Therapon modeling clastogenicitypeople reside withinand genotoxicity Lam Takong in fishRiver and basin. mussels The jarbuahas become belongs a primary to the technology order Perciformes for analyzing of theNPS (Dailianisriver is 220et km al., long 2003). and originates from Sangampang familypollution Theraponidae. and its spatial Thedistribution fish species, (Chu et Therapon al., 2004). RangeThe in MN Khao tests Yai Nationalhave been Park. successfully It covers about used nine as jarbuaExamples(6-6.3 of thesecm in models length andare: 4-4.25the physically g in weight) based a measure of genotoxic stress in fish, under both was selected for the detection of genotoxic effect N. Pongpetch et al. / EnvironmentAsia 8(1) (2015) 41-52 event Areal Non-point Source Watershed Environment Mueang Nakhon Ratchasima and Chaloem Phra Response Simulation (ANSWERS) model, the Kiat districts in Nakhon Ratchasima province (Royal physically based Dynamic Watershed Simulation Model Irrigation Department, 2004). At Ban Kong Rae, Kham (DWSM) model and the Soil and Water Assessment Thale So district, Lam Boriboon River diverges from Tool (SWAT) model. When comparing the models, Lam Takong River for 35 km and then rejoins Lam SWAT is the most capable for long-term simulations Takong River at Ban Kanphom before flowing into the in watersheds dominated by agricultural land (Arnold Moon River at Tha Chang Sub district, Chaloem Phra and Fohrer, 2005; Arnold et al., 1998). It is designed to Kiat district in Nakhon Ratchasima province (Nakhon assess the impact of land use and management practices Ratchasima Municipality, 2006). on water, sediments and agricultural chemicals. The Lam Takong River basin is influenced by the model has proven to be an effective tool for assessing southwest and northeast monsoons. There are three NPS for a wide range of scales and environmental seasons including rainy, winter and summer seasons. conditions
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