DOCSLIB.ORG

Hplc∓Dad∓ESI-MS/MS Screening of Bioactive Components

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Hplc∓Dad∓ESI-MS/MS Screening of Bioactive Components Food Chemistry 166 (2015) 179–191 Contents lists available at ScienceDirect Food Chemistry journal homepage: www.elsevier.com/locate/foodchem HPLC–DAD–ESI-MS/MS screening of bioactive components from Rhus coriaria L. (Sumac) fruits ⇑ Ibrahim M. Abu-Reidah a,b,c, Mohammed S. Ali-Shtayeh a, , Rana M. Jamous a, David Arráez-Román b,c, ⇑ Antonio Segura-Carretero b,c, a Biodiversity & Environmental Research Center (BERC), Til, Nablus POB 696, Palestine b Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva, 18071 Granada, Spain c Functional Food Research and Development Centre (CIDAF), PTS Granada, Avda. del Conocimiento, Edificio Bioregión, 18016 Granada, Spain article info abstract Article history: Rhus coriaria L. (sumac) is an important crop widely used in the Mediterranean basin as a food spice, and Received 25 March 2014 also in folk medicine, due to its health-promoting properties. Phytochemicals present in plant foods are in Received in revised form 29 May 2014 part responsible for these consequent health benefits. Nevertheless, detailed information on these Accepted 3 June 2014 bioactive compounds is still scarce. Therefore, the present work was aimed at investigating the Available online 12 June 2014 phytochemical components of sumac fruit epicarp using HPLC–DAD–ESI-MS/MS in two different ionisation modes. The proposed method provided tentative identification of 211 phenolic and other Keywords: phyto-constituents, most of which have not been described so far in R. coriaria fruits. More than 180 Palestinian sumac phytochemicals (tannins, (iso)flavonoids, terpenoids, etc.) are reported herein in sumac fruits for the first Anacardiaceae Hydrolysable tannins time. The obtained results highlight the importance of R. coriaria as a promising source of functional Flavonoids ingredients, and boost its potential use in the food and nutraceutical industries. Mediterranean diet Ó 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license HPLC–DAD–ESI-MS/MS (http://creativecommons.org/licenses/by-nc-nd/3.0/). 1. Introduction is known to possess non-mutagenic, fever-reducing, DNA protec- tive, antiseptic, antifungal, antibacterial, antioxidant, anti-ischae- Sumac, Rhus coriaria L. (Anacardiaceae), is a wild edible plant mic, hypouricemic, hypoglycaemic, and hepatoprotective growing in the Mediterranean region, has long been used as a properties, which support its traditional uses (Anwer et al., 2013; seasoning spice, either in pure form or in combination with other Chakraborty et al., 2009; Madihi et al., 2013; Shafiei et al., 2011). spices (Ali-Shtayeh, & Jamous, 2008), sauce, appetizer, drink, and Among 56 Palestinian plants tested, sumac was found to have as a souring agent in food recipes. R. coriaria L. is an important the greatest antimicrobial effect against Probionibacterium acnes and the most widely used species of the genus Rhus in the (MIC 6 mg/ml, MBC 6 mg/ml), Staphylococcus aureus (MIC 4 mg/ Mediterranean region since antiquity. Recently, the consumption ml, MBC 6 mg/ml), Escherichia coli (MIC 6 mg/ml, MBC 8 mg/ml) of sumac fruits has been increasing around the world as an and Pseudomonas aeruginosa (MIC 4 mg/ml and MBC 6 mg/ml) important economic crop (Kizil, & Turk, 2010). (Ali-Shtayeh, Al-Assali, & Jamous, 2013). In folk medicine and traditional Arabic Palestinian herbal med- The literature lacks detailed information on R. coriaria chemical icine, this plant has been used in the treatment of cancer, stroke, composition. Previous works have reported sumac to contain phe- diarrhoea, hypertension, dysentery, haematemesis, ophthalmia, nolic compounds, such as hydrolysable tannins, anthocyanins and stomach ache, diuresis, diabetes, atherosclerosis, measles, small- also organic acids such as malic and citric acids (Kosar, Bozan, pox, liver disease, aconuresis, teeth and gum ailments, headaches, Temelli, & Baser, 2007; Kossah, Nsabimana, Zhang, & Chen, animal bites, dermatitis, and liver disease (Ali-Shtayeh, & Jamous, 2010). Interestingly, the acidic and astringent tastes, may be due 2008; Shafiei, Nobakht, & Moazzam, 2011). Furthermore, R. coriaria to indigenous organic acids (mainly, malic acid) and tannins. Many compounds have been identified from different parts of sumac, such as phenolics, organic acids, proteins, fibre, volatile oils, fatty ⇑ Corresponding authors. Tel.: +970 92536406 (M.S. Ali-Shtayeh). Address: acids, vitamins, and minerals (Anwer et al., 2013; Özcan, & Functional Food Research and Development Center (CIDAF), PTS Granada, Avda. Haciseferogullari, 2004). Only a few studies have been carried del, Conocimiento, Edificio Bioregión, 18016 Granada, Spain. Tel.: +34 958248435 out on the chemical composition of R. coriaria leaves (Regazzoni (A. Segura-Carretero). E-mail addresses: [email protected] (M.S. Ali-Shtayeh), [email protected] et al., 2013; Van Loo, De Bruyn, & Verzele, 1988) and little is known (A. Segura-Carretero). about the phytochemical composition of the plant’s fruit epicarps. http://dx.doi.org/10.1016/j.foodchem.2014.06.011 0308-8146/Ó 2014 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). 180 I.M. Abu-Reidah et al. / Food Chemistry 166 (2015) 179–191 Although R. coriaria is a particularly rich source of phenolic Technologies, Santa Clara, CA) consisting of a vacuum degasser, compounds (Kossah et al., 2010), the phenolic constituents of an auto-sampler, a binary pump and diode-array detector (DAD). sumac fruit’s epicarp remains so far incompletely investigated. This instrument was equipped with an Agilent Zorbax C18 column Thus, detailed and extended profiling of the phytochemicals of (4.6  150 mm, 5 lm) from Agilent Technologies. Acidified water sumac fruits using high sensitive tools is necessary. Consequently, (0.5% acetic acid, v/v) and acetonitrile were used as mobile phases suitable methods need to be established for the identification of A and B, respectively. The gradient was programmed as follows: phytochemicals in plant food matrices (Abu-Reidah, Contreras, 0 min, 0% B; 20 min, 20% B; 30 min, 30% B; 40 min, 50% B; Arráez-Román, Fernández-Gutiérrez, & Segura-Carretero, 2014). 50 min, 75% B; 60 min, 100% B; 62 min 0% B, and finally, the initial Mass spectrometry coupled to high-performance liquid chroma- conditions were held for 8 min as a re-equilibration step. The flow tography (HPLC–MS) has been increasingly used in the structural rate was set at 0.80 mL/min throughout the gradient. The flow characterisation of complex matrices and has proved to be the from the HPLC system into the ESI-Q-TOF-MS detector was tool of choice to identify phenolic compounds (Abu-Reidah, 0.2 mL/min. The injection volume was 10 lL and the column Arráez-Román, Lozano-Sánchez, Segura-Carretero, & Fernández- temperature was maintained at 25 °C. Gutiérrez, 2013; Abu-Reidah, Arráez-Román, Segura-Carretero, & The HPLC system was coupled to a quadrupole-time-of-flight Fernández-Gutiérrez, 2013; Lee, Zweigenbaum, & Mitchell, 2013). (micrOTOF-Q™, Bruker Daltonik GmbH, Bremen, Germany) Therefore, the objective of the present study was to investigate orthogonal accelerated Q-TOF mass spectrometer, equipped with the phytochemical composition of hydro-methanolic extracts of R. an electrospray ionisation source (ESI). Parameters for analysis coriaria fruits cultivated in Palestine, by using high-performance were set using negative and positive ion modes, with spectra liquid chromatography-diode array detector-hyphenated with acquired over a mass range from m/z 50 to 1100. The optimum val- tandem mass spectrometry (HPLC–DAD–ESI-MS/MS) as a potent ues of the ESI-MS parameters were: capillary voltage, À3.5 and analytical technique. +4.0 kV; drying gas temperature, 190 °C; drying gas flow, 9.0 L/ min; nebulising gas pressure, 29 psi; collision RF, 150 Vpp; transfer 2. Materials and methods time 70 ls, and pre-pulse storage, 5 ls. Moreover, automatic MS/ MS experiments were performed adjusting the collision energy 2.1. Chemicals values as follows: m/z 100, 20 eV; m/z 500, 30 eV; m/z 1000, 35 eV, using nitrogen as collision gas. The MS data were processed Acetonitrile and methanol of analytical or HPLC grade were pur- through Data Analysis 4.0 software (Bruker Daltonics, Bremen, chased from Labscan (Dublin, Ireland). Acetic acid of analytical Germany) which provided a list of possible elemental formulas grade (assay >99.5%) was purchased from Fluka (Switzerland). by using the Generate Molecular Formula™ editor. The editor uses Water was purified by using a Milli-Q system (Millipore, Bedford, a CHNO algorithm, which provides standard functionalities, such USA). as maximum/minimum elemental range, and a sophisticated com- parison of the theoretical with the measured isotope pattern (mSigma value), for increasing the confidence in the suggested 2.2. Sample preparation molecular formula. The widely accepted accuracy for confirmation of elemental compositions has been established as 5 ppm. Sumac is commercially obtainable in local markets in ready- At some stage in the HPLC method development, an external to-use ground form. In our present study, for quality control apparatus calibration was performed using a Cole Palmer syringe considerations, sumac samples were harvested in their mature pump (Vernon Hills, IL) directly linked to the interface, passing a stage from the wild habitat mountains of Nablus (Qusra village) solution of sodium acetate. Using this method, an exact calibration in summer of 2012 and were identified by Prof. Mohammad curve based on numerous cluster masses each differing by 82 Da S. Ali-Shtayeh from BERC. Collected sumac samples were dried, (C2H3NaO2) was obtained. Due to the compensation of tempera- and then epicarps of R. coriaria L. fruits were liberated from kernels ture drift in the Q-TOF, this external calibration provided accurate and ground into powder using a household mill and stored at room mass values for a complete run without the need for a dual sprayer temperature until they were used for extraction.
Load more

Recommended publications
  • 100% Natural Birch Tree Chaga Mushrooms
    2/28/2018 100% NATURAL BIRCH TREE CHAGA MUSHROOMS Grown in the Pristine Forests of Northern Canada 100% NATURAL BIRCH TREE CHAGA MUSHROOMS GROWN IN THE PRISTINE FORESTS OF NORTHERN CANADA Chaga is a highly nutritious mushroom that grows on birch trees in below freezing temperatures. Semintha Chaga grows at -40°C in a primeval organic environment clear of any nuclear waste, contaminants or other toxins making Semintha chaga mushrooms safe for chronic consumption and highly effective. Chaga is the dense black mass (25-40 cm large) that can be seen on the outside of birch trees. It is a dense sterile mass of mycelia, with decayed bits of birch tissue incorporated. They are quite rare and difficult to harvest. When chopped from the tree the interior has a rusty yellow-brown color, somewhat granular in appearance, and is often mottled with whitish or cream-colored veins. The hard, deeply cracked black outside of the Chaga is called the sclerotium. Mature Chaga sclerotia are found on trees over 40 years of age. The estimated time period between the times of infection of the tree by the fungus to the maturity of the chaga mushroom is around 20 years. The chaga can be harvested five years post maturity. After harvesting, chaga can regrow to harvestable size again in three to ten years, and this can be repeated until the tree dies. SUPPLEMENT FACTS Serving size 3gr Total Fat 0.03g Total Carb 2.25g Cholesterol 0mg Protein 12g Sodium 0.42mg Calcium 1.44mg Magnesium 1.4mg Manganese 0.21mg Potassium 51mg Sodium 0.011mg Phosphorus 9,96mg Iron 0,07mg Beta Glucan 0,75g Zinc 0,12mg 1 OF 14 BENEFICIAL HEALTH PROPERTIES OF CHAGA MUSHROOMS Chaga Mushrooms are adaptogens, high in polysaccharides, alkalines, phytonutrients, organic acids, calcium, zinc, magnesium, chromium and other important minerals and microelements.
    [Show full text]
  • Anti-Inflammatory Activity of Plant Polyphenols
    biomedicines Editorial Special Issue: Anti-Inflammatory Activity of Plant Polyphenols Enrico Sangiovanni and Mario Dell’Agli * Department of Pharmacological and Biomolecular Sciences, Università degli Studi di Milano, Via Balzaretti 9, 20133 Milano, Italy; [email protected] * Correspondence: [email protected]; Tel.: +3902-5031-8398 Received: 5 March 2020; Accepted: 13 March 2020; Published: 18 March 2020 1. Introduction Inflammation is considered the first physiological response of the human body to infection or injury, playing a critical role in both innate and adaptive immunity. It is characterized by the complex biological response of vascular tissues to harmful stimuli, such as pathogens, damaged or tumoral cells, or irritants. Uncontrolled inflammation often results in chronic diseases, such as gastritis, arthritis, autoimmune disorders, degenerative joint diseases, rheumatisms, atherosclerosis, diabetes, and certain cancers. The inflammatory process is characterized by the migration of immune cells from blood vessels to the site of inflammation, with massive release of pro-inflammatory mediators, including cytokines, chemokines, prostaglandins, leukotrienes, and oxidative agents such as reactive oxygen species (ROS) above all. The search for new strategies which are able to interfere with these mechanisms by preventing a prolonged inflammation would greatly benefit large number of subjects. In this respect, the plant kingdom has developed a multitude of secondary metabolites, many of which are recognized as useful tools
    [Show full text]
  • The Wood Cross Sections of Hermann Nördlinger (1818–1897)
    IAWA Journal, Vol. 29 (4), 2008: 439–457 THE WOOD CROSS SECTIONS OF HERMANN NÖRDLINGER (1818–1897) Ben Bubner Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF) e.V., Institut für Landschaftsstoffdynamik, Eberswalder Str. 84, 15374 Müncheberg, Germany [E-mail: [email protected]] SUMMARY Hermann Nördlinger (1818–1897), forestry professor in Hohenheim, Germany, published a series of wood cross sections in the years 1852 to 1888 that are introduced here to the modern wood anatomist. The sec- tions, which vary from 50 to 100 μm in thickness, are mounted on sheets of paper and their quality is high enough to observe microscopic details. Their technical perfection is as remarkable as the mode of distribution: sections of 100 wood species were presented in a box together with a booklet containing wood anatomical descriptions. These boxes were dis- tributed as books by the publisher Cotta, from Stuttgart, Germany, with a maximum circulation of 500 per volume. Eleven volumes comprise 1100 wood species from all over the world. These include not only conifers and broadleaved trees but also shrubs, ferns and palms representing a wide variety of woody structures. Excerpts of this collection were also pub- lished in Russian, English and French. Today, volumes of Nördlingerʼs cross sections are found in libraries throughout Europe and the United States. Thus, they are relatively easily accessible to wood anatomists who are interested in historic wood sections. A checklist with the content of each volume is appended. Key words: Cross section, wood collection, wood anatomy, history. INTRODUCTION Wood scientists who want to distinguish wood species anatomically rely on thin sec- tions mounted on glass slides and descriptions in books that are illustrated with micro- photographs.
    [Show full text]
  • Rhus Coriaria
    Rhus coriaria (Elm-Leaved Sumach, Sicilian sumac) " Every Lebanese has eaten the dark red condiment called summac, which is derived from the fruits of this tree and is used in popular foods such as the Mankouche, Kaakeh, and Fattoush.Summac tree is found in every part of the country, growing at almost every altitude. This small tree, composed of single or multiple slender trunks, reaches 2 to 3 meters in height.The Summac tree can grow in poor, rocky soils with little access to water; it is tolerant to drought and grows well along the coast, because it is also resistant to salt." from: Trees of Lebanon, 2014, Salma Nashabe Talhouk, Mariana M. Yazbek, Khaled Sleem, Arbi J. Sarkissian, Mohammad S. Al-Zein, and Sakra Abo Eid Landscape Information French Name: Le Sumac des corroyeurs ﺳﻤﺎﻕ :Arabic Name Plant Type: Shrub Origin: Southern Europe Heat Zones: 4, 5, 6, 7, 8 Hardiness Zones: 8, 9, 10, 11 Uses: Medicinal, Native to Lebanon Size/Shape Growth Rate: Moderate Tree Shape: Spreading Canopy Symmetry: Irregular Canopy Density: Dense Plant Image Canopy Texture: Medium Height at Maturity: 1.5 to 3 m Spread at Maturity: 3 to 5 meters Time to Ultimate Height: 10 to 20 Years Rhus coriaria (Elm-Leaved Sumach, Sicilian sumac) Botanical Description Foliage Leaf Arrangement: Whorled Leaf Venation: Pinnate Leaf Persistance: Deciduous Leaf Type: Odd Pinnately compund Leaf Blade: 5 - 10 cm Leaf Shape: Oval Leaf Margins: Serrate Leaf Textures: Hairy, Fine Leaf Scent: No Fragance Color(growing season): Green Color(changing season): Green Flower
    [Show full text]
  • Field Guide – Common Trees and Shrubs of Georgia
    Introduction Up to 400 species of trees and shrubs grow in Georgian for- ests. This Field Guide contains information about 100 species of trees and shrubs from 38 plant families. The abundance of relict and endemic timber species (61 species endemic to Geor- gia and 43 species endemic to the Caucasus) indicates the high biodiversity of Georgian forests. Georgian forests provide habitats and migration corridors to a range of wild fauna, and play an important role in the conserva- tion of the genetic diversity of animal species in the region. In conditions of complex and deeply dissected relief, characteristic to Georgia, forests are especially important due to their climate regulation, water regulation and soil protection functions. Forests also ensure the continuous delivery of vital benefits and resources to the population, and facilitate the development of a range of industries. Introduction In this Field Guide each plant family is displayed in a different color. The Field Guide contains an alphabetical index of species, as well as the names of species in Latin and English, as estab- lished by the International Code of Botanical Nomenclature. The Field Guide also contains a brief description of the taxo- nomic characteristics, range and protection status of each spe- cies. Alphabetical Index Name in English Name in Latin # Alpine Currant Ribes alpinum 59 Bay Laurel Laurus nobilis 62 Begonia-Leafed Lime Tilia Begoniifolia 92 Bitchvinta Pine Pinus pithyusa 6 Black Alder Alnus barbata 28 Black Elder Sambucus nigra 31 Black Poplar Populus
    [Show full text]
  • Molecular Docking Study on Several Benzoic Acid Derivatives Against SARS-Cov-2
    molecules Article Molecular Docking Study on Several Benzoic Acid Derivatives against SARS-CoV-2 Amalia Stefaniu *, Lucia Pirvu * , Bujor Albu and Lucia Pintilie National Institute for Chemical-Pharmaceutical Research and Development, 112 Vitan Av., 031299 Bucharest, Romania; [email protected] (B.A.); [email protected] (L.P.) * Correspondence: [email protected] (A.S.); [email protected] (L.P.) Academic Editors: Giovanni Ribaudo and Laura Orian Received: 15 November 2020; Accepted: 1 December 2020; Published: 10 December 2020 Abstract: Several derivatives of benzoic acid and semisynthetic alkyl gallates were investigated by an in silico approach to evaluate their potential antiviral activity against SARS-CoV-2 main protease. Molecular docking studies were used to predict their binding affinity and interactions with amino acids residues from the active binding site of SARS-CoV-2 main protease, compared to boceprevir. Deep structural insights and quantum chemical reactivity analysis according to Koopmans’ theorem, as a result of density functional theory (DFT) computations, are reported. Additionally, drug-likeness assessment in terms of Lipinski’s and Weber’s rules for pharmaceutical candidates, is provided. The outcomes of docking and key molecular descriptors and properties were forward analyzed by the statistical approach of principal component analysis (PCA) to identify the degree of their correlation. The obtained results suggest two promising candidates for future drug development to fight against the coronavirus infection. Keywords: SARS-CoV-2; benzoic acid derivatives; gallic acid; molecular docking; reactivity parameters 1. Introduction Severe acute respiratory syndrome coronavirus 2 is an international health matter. Previously unheard research efforts to discover specific treatments are in progress worldwide.
    [Show full text]
  • Antioxidant Activity of Polyphenolic Plant Extracts
    Antioxidant Activity Antioxidant Extracts Polyphenolic Plant of • Dimitrios Stagos Antioxidant Activity of Polyphenolic Plant Extracts Edited by Dimitrios Stagos Printed Edition of the Special Issue Published in Antioxidants ww.mdpi.com/journal/antioxidants Antioxidant Activity of Polyphenolic Plant Extracts Antioxidant Activity of Polyphenolic Plant Extracts Editor Dimitrios Stagos MDPI • Basel • Beijing • Wuhan • Barcelona • Belgrade • Manchester • Tokyo • Cluj • Tianjin Editor Dimitrios Stagos University of Thessaly Greece Editorial Office MDPI St. Alban-Anlage 66 4052 Basel, Switzerland This is a reprint of articles from the Special Issue published online in the open access journal Antioxidants (ISSN 2076-3921) (available at: https://www.mdpi.com/journal/antioxidants/special issues/Polyphenolic Plant Extracts). For citation purposes, cite each article independently as indicated on the article page online and as indicated below: LastName, A.A.; LastName, B.B.; LastName, C.C. Article Title. Journal Name Year, Volume Number, Page Range. ISBN 978-3-0365-0288-5 (Hbk) ISBN 978-3-0365-0289-2 (PDF) © 2021 by the authors. Articles in this book are Open Access and distributed under the Creative Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications. The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons license CC BY-NC-ND. Contents About the Editor .............................................. ix Preface to ”Antioxidant Activity of Polyphenolic Plant Extracts” .................. xi Dimitrios Stagos Antioxidant Activity of Polyphenolic Plant Extracts Reprinted from: Antioxidants 2020, 9, 19, doi:10.3390/antiox9010019 ................
    [Show full text]
  • Specimen Label
    Specimen Label ®Trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow For control of susceptible weeds and certain woody plants, including invasive and noxious weeds, on rangeland, permanent grass pastures, Conservation Reserve Program (CRP) acres, non-cropland areas including industrial sites, rights-of-way (such as roadsides, electric utility and communication transmission lines, pipelines, and railroads), non-irrigation ditch banks, natural areas (such as wildlife management areas, wildlife openings, wildlife habitats, recreation areas, campgrounds, trailheads and trails), and grazed areas in and around these sites. Hay from grass treated with Opensight within the preceding 18-months can only be used on the farm or ranch where product is applied unless allowed by supplemental labeling IMPORTANT USE PRECAUTIONS AND RESTRICTIONS TO PREVENT INJURY TO DESIRABLE PLANTS • Carefully read the section “Restrictions in Hay or Manure Use .” • It is mandatory to follow the “Use Precautions and Restrictions” section of this label. • Manure and urine from animals consuming grass or hay treated with this product may contain enough aminopyralid to cause injury to sensitive broadleaf plants. • Hay can only be used on the farm or ranch where product is applied unless allowed by supplemental labeling. • Consult with a Dow AgroSciences representative if you do not understand the “Use Precautions and Restrictions”. Call [1-(800) 263-1196] Customer Information Group. ©Copyright 2011 Dow AgroSciences LLC Not For Sale, Distribution,
    [Show full text]
  • Evolution of 49 Phenolic Compounds in Shortly-Aged Red Wines Made from Cabernet Gernischt (Vitis Vinifera L
    Food Sci. Biotechnol. Vol. 18, No. 4, pp. 1001 ~ 1012 (2009) ⓒ The Korean Society of Food Science and Technology Evolution of 49 Phenolic Compounds in Shortly-aged Red Wines Made from Cabernet Gernischt (Vitis vinifera L. cv.) Zheng Li, Qiu-Hong Pan, Zan-Min Jin, Jian-Jun He, Na-Na Liang, and Chang-Qing Duan* Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China Abstract A total of 49 phenolic compounds were identified from the aged red wines made from Cabernet Gernischt (Vitis vinifera L. cv.) grapes, a Chinese characteristic variety, including 13 anthocyanins, 4 pryanocyanins, 4 flavan-3-ol monomers, 6 flavan-3-ol polymers, 7 flavonols, 6 hydroxybenzoic acids, 5 hydroxycinnamic acids, 3 stilbenes, and 1 polymeric pigment. Evolution of these compounds was investigated in wines aged 1 to 13 months. Variance analysis showed that the levels of most phenolics existed significant difference in between wines aged 3 and 9 months. Cluster analysis indicated that 2 groups could be distinguished, one corresponding to wines aged 1 to 3 months and the other to wines aged 4 to 13 months. It was thus suggested that there were 2 key-stages for the development of fine wine quality, at the aged 3 and 9 months, respectively. This work would provide some helpful information for quality control in wine production. Keywords: Cabernet Gernischt, aged wine, phenolic compound, evolution, high performance liquid chromatography coupled with tandem mass spectrometry Introduction 3-ols in red wine and correlation with wine age (12), the evolutions of low molecular weight phenolic compounds Phenolic compounds in red wines mainly contain such as gallic acids and caffeic acids (13,14), as well as the anthocyanins, flavonols, flavan-3-ols, phenolic acids effects of oak barrel compounds and sorption behaviors on (including hydroxybenzoic acids and hydroxycinnamic evolution of phenolic compounds (15), have been reported.
    [Show full text]
  • Preparation of Glass-Ionomer Cement Containing Ethanolic Brazilian
    www.nature.com/scientificreports OPEN Preparation of glass‑ionomer cement containing ethanolic Brazilian pepper extract (Schinus terebinthifolius Raddi) fruits: chemical and biological assays Isabelle C. Pinto1, Janaína B. Seibert2, Luciano S. Pinto2, Vagner R. Santos3, Rafaela F. de Sousa1, Lucas R. D. Sousa1,4, Tatiane R. Amparo5, Viviane M. R. dos Santos1, Andrea M. do Nascimento1, Gustavo Henrique Bianco de Souza5, Walisson A. Vasconcellos6, Paula M. A. Vieira4 & Ângela L. Andrade1* Plants may contain benefcial or potentially dangerous substances to humans. This study aimed to prepare and evaluate a new drug delivery system based on a glass‑ionomer‑Brazilian pepper extract composite, to check for its activity against pathogenic microorganisms of the oral cavity, along with its in vitro biocompatibility. The ethanolic Brazilian pepper extract (BPE), the glass‑ionomer cement (GIC) and the composite GIC‑BPE were characterized by scanning electron microscopy, attenuated total refectance Fourier transform infrared spectroscopy (ATR‑FTIR), and thermal analysis. The BPE compounds were identifed by UPLC–QTOF–MS/MS. The release profle of favonoids and the mechanical properties of the GIC‑BPE composite were assessed. The favonoids were released through a linear mechanism governing the difusion for the frst 48 h, as evidenced by the Mt/M∞ relatively to √t , at a difusion coefcient of 1.406 × 10–6 cm2 s−1. The ATR‑FTIR analysis indicated that a chemical bond between the GIC and BPE components may have occurred, but the compressive strength of GIC‑BPE does not difer signifcantly from that of this glass‑ionomer. The GIC‑BPE sample revealed an ample bacterial activity at non‑cytotoxic concentrations for the human fbroblast MRC‑5 cells.
    [Show full text]
  • Antioxidant, Cytotoxic, and Antimicrobial Activities of Glycyrrhiza Glabra L., Paeonia Lactiflora Pall., and Eriobotrya Japonica (Thunb.) Lindl
    Medicines 2019, 6, 43; doi:10.3390/medicines6020043 S1 of S35 Supplementary Materials: Antioxidant, Cytotoxic, and Antimicrobial Activities of Glycyrrhiza glabra L., Paeonia lactiflora Pall., and Eriobotrya japonica (Thunb.) Lindl. Extracts Jun-Xian Zhou, Markus Santhosh Braun, Pille Wetterauer, Bernhard Wetterauer and Michael Wink T r o lo x G a llic a c id F e S O 0 .6 4 1 .5 2 .0 e e c c 0 .4 1 .5 1 .0 e n n c a a n b b a r r b o o r 1 .0 s s o b b 0 .2 s 0 .5 b A A A 0 .5 0 .0 0 .0 0 .0 0 5 1 0 1 5 2 0 2 5 0 5 0 1 0 0 1 5 0 2 0 0 0 1 0 2 0 3 0 4 0 5 0 C o n c e n tr a tio n ( M ) C o n c e n tr a tio n ( M ) C o n c e n tr a tio n ( g /m l) Figure S1. The standard curves in the TEAC, FRAP and Folin-Ciocateu assays shown as absorption vs. concentration. Results are expressed as the mean ± SD from at least three independent experiments. Table S1. Secondary metabolites in Glycyrrhiza glabra. Part Class Plant Secondary Metabolites References Root Glycyrrhizic acid 1-6 Glabric acid 7 Liquoric acid 8 Betulinic acid 9 18α-Glycyrrhetinic acid 2,3,5,10-12 Triterpenes 18β-Glycyrrhetinic acid Ammonium glycyrrhinate 10 Isoglabrolide 13 21α-Hydroxyisoglabrolide 13 Glabrolide 13 11-Deoxyglabrolide 13 Deoxyglabrolide 13 Glycyrrhetol 13 24-Hydroxyliquiritic acid 13 Liquiridiolic acid 13 28-Hydroxygiycyrrhetinic acid 13 18α-Hydroxyglycyrrhetinic acid 13 Olean-11,13(18)-dien-3β-ol-30-oic acid and 3β-acetoxy-30-methyl ester 13 Liquiritic acid 13 Olean-12-en-3β-ol-30-oic acid 13 24-Hydroxyglycyrrhetinic acid 13 11-Deoxyglycyrrhetinic acid 5,13 24-Hydroxy-11-deoxyglycyirhetinic
    [Show full text]
  • Rhamnus Prinoides Plant Extracts and Pure Compounds Inhibit Microbial Growth and Biofilm Ormationf
    Georgia State University ScholarWorks @ Georgia State University Biology Dissertations Department of Biology 12-15-2020 Rhamnus prinoides Plant Extracts and Pure Compounds Inhibit Microbial Growth and Biofilm ormationF Mariya Campbell Follow this and additional works at: https://scholarworks.gsu.edu/biology_diss Recommended Citation Campbell, Mariya, "Rhamnus prinoides Plant Extracts and Pure Compounds Inhibit Microbial Growth and Biofilm ormation.F " Dissertation, Georgia State University, 2020. https://scholarworks.gsu.edu/biology_diss/246 This Dissertation is brought to you for free and open access by the Department of Biology at ScholarWorks @ Georgia State University. It has been accepted for inclusion in Biology Dissertations by an authorized administrator of ScholarWorks @ Georgia State University. For more information, please contact [email protected]. RHAMNUS PRINOIDES PLANT EXTRACTS AND PURE COMPOUNDS INHIBIT MICROBIAL GROWTH AND BIOFILM FORMATION by MARIYA M. CAMPBELL Under the Direction of Eric Gilbert, PhD ABSTRACT The increased prevalence of antibiotic resistance threatens to render all of our current antibiotics ineffective in the fight against microbial infections. Biofilms, or microbial communities attached to biotic or abiotic surfaces, have enhanced antibiotic resistance and are associated with chronic infections including periodontitis, endocarditis and osteomyelitis. The “biofilm lifestyle” confers survival advantages against both physical and chemical threats, making biofilm eradication a major challenge. A need exists for anti-biofilm treatments that are “anti-pathogenic”, meaning they act against microbial virulence in a non-biocidal way, leading to reduced drug resistance. A potential source of anti-biofilm, anti-pathogenic agents is plants used in traditional medicine for treating biofilm-associated conditions. My dissertation describes the anti-pathogenic, anti-biofilm activity of Rhamnus prinoides (gesho) extracts and specific chemicals derived from them.
    [Show full text]