JNCASR
IISc Mathematics Initiative and International Network in Theoretical Immunology present an International meeting
Theoretical and Experimental Immunology
August 16, 2011 Faculty Hall, Indian Institute of Science (IISc) WITH BEST COMPLIMENTS
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August 16, 2011 Faculty Hall, Indian Institute of Science (IISc)
GENERAL INSTRUCTIONS
1. Students who have registered “on line” are requested to arrive on August 16, 2011 at 8: 30 am in the Faculty Hall, IISc to collect their registration materials.
2. Do note that outstation students will need to make their own arrangements for stay. The website does list reasonable places to stay in Bangalore.
3. Traffic in Bangalore is chaotic, so please give yourself sufficient time to ensure that you are not late for the meeting.
4. Please display your badge all the time. Entry into the auditoriums, food areas etc. will NOT be permitted without the badge.
5. Kindly switch off your mobile inside the auditorium.
6. Speakers are requested to load their slides prior to the session. Student volunteers will help you in the process. Kindly preview your slides before the presentation.
7. The meeting schedule is rather tight and we kindly request all speakers and chairpersons to please keep to time.
8. For any information, clarifications and assistance, please contact local organization committee members and student volunteers at the registration desk.
iii iv CONTENTS
General Instructions iii
MAP of IISc iv
Contents v
Meeting Organization vi
Message vii
Program ix
Abstracts 1
List of Speakers 18
List of Participants 19
Honored Guests 29
List of Company Sponsors 34
v MEETING ORGANIZATION
Govindan Rangarajan (Chairman) Sathees C. Raghavan Department of Mathematics Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] [email protected]
Dipankar Nandi (Convener) Deepak K. Saini Department of Biochemistry Department of Molecular Reproduction, Indian Institute of Science Development and Genetics [email protected] Indian Institute of Science [email protected] Anjali A. Karande Department of Biochemistry Parag P. Sadhale Indian Institute of Science Department of Microbiology and [email protected] Cell Biology Indian Institute of Science R. Manjunath [email protected] Department of Biochemistry Indian Institute of Science Dipshikha Chakravortty [email protected] Department of Microbiology and Cell Biology Narendra M. Dixit Indian Institute of Science Department of Chemical Engineering [email protected] Indian Institute of Science [email protected] S.G. Ramachandra Central Animal facility Soumyendu Raha Indian Institute of Science SERC [email protected] Indian Institute of Science [email protected] William R. Surin FACS Facility Udaykumar Ranga Indian Institute of Science MBGU, JNCASR [email protected] [email protected]
vi MESSAGE
We are delighted that a meeting on “Theoretical and Experimental Immunology” is being held in the Indian Institute of Science, Bangalore. This meeting is certainly a unique experiment of its kind – but one whose time has probably come! Immunology, in general, is the study of the host defense network against infections, tumors, allergies etc. Experimental immunology is well studied and has shed light on the inner workings of immune cells. Studies in this field have led to a better understanding of the host defense network. On the other hand, theoretical immunology, utilizing mathematical tools, is a relatively newer area of study. Theoretical immunology involves the development of mathematical and computational models of biological phenomena that unravel, in synergy with experiments, new insights into the workings of our immune system. We are happy that this meeting has brought in top notch researchers in the fields of theoretical and experimental immunology.
It is heartening to know that the response to this specialized meeting has been good with over 150 student registrations from different parts of the country. Students are encouraged to take the maximum advantage and interact with faculty present in this meeting. To ensure that students get the maximum advantage, speakers have been requested to ensure that the talks are pedagogic in nature. The first half may give the audience an overview of the field and the second half may focus on the speaker’s research contributions. We are hopeful that this meeting will seek answers to questions, which are at the heart of our understanding of Immunology, and may pave the way for the design of potent adjuvants and vaccines.
This meeting has been possible due to the active participation of several people and institutions. We thank IISc for permission to use the various facilities required to conduct this meeting. The support from the Department of Mathematics and the Department of Biochemistry, IISc is greatly appreciated. The generous financial assistance from JNCASR, Centre for Scientific & Industrial Consultancy, IISc and a large number of private companies is greatly appreciated. We thank Carmen Molina-Paris and Grant Lythe for suggesting Bangalore to be the venue for this year’s annual meeting of the
vii International Network in Theoretical Immunology (INTI). We appreciate the support from other INTI members.
The staff of the IISc Mathematics Initiative (IMI) has played a stellar role in setting up the web site, taking care of general administrative duties and finances. We thank Grant Lythe, Srabanti Rakshit and Mukta Deobagkar for allowing their images to adorn the posters and Abstract book for this meeting. We appreciate the help of Emmanuel Stephen and Rajkumar D. from D. Nandi’s laboratory. In addition, several faculty and students from the Division of Biological Sciences, IISc have contributed to the organization of this meeting for which we are extremely thankful.
Finally, we welcome you to this event in Bangalore and look forward to your active participation in making this meeting a resounding success!
With warm wishes,
G. Rangarajan Dipankar Nandi Chairperson Convenor
viii PROGRAM
Theoretical and Experimental Immunology Meeting (TEIM)
Faculty Hall Indian Institute of Science, Aug 16, 2011 Bangalore
8:30 am – 9:15 am: Registration
9:15 am Introductory remarks by G. Rangarajan, Chairperson, TEIM
Welcome by D. N. Rao, Divisional Chairman, Division of Biological Sciences, IISc
Vote of thanks by D. Nandi, Convenor, TEIM
9:30 am PLENARY TALK Chairperson: G. Rangarajan, Indian Institute of Science, Bangalore
Development and maintenance of the T cell pool in health and disease Robin Callard Institute of Child Health, London, UK
10: 10 am – 10:30 am: TEA
SESSION 1 Chairperson: Carmen Molina-Paris, Leeds University
10:30 am – 10:55 am: Studies on the dynamics of germinal centers J. Faro University of Vigo, Spain
10: 55 am – 11: 20 am: Agent based simulation of T cell based immune response: modeling an adoptive transfer experiment Gib Bogle University of Auckland, New Zealand
11: 20 am – 11: 45 am: T cells and ageing: effects of age on survival and function Vineeta Bal NII, New Delhi, India
ix SESSION II Chairperson: Anjali Karande
11: 45 am – 12:10 pm: Brownian motion and the adaptive immune system Grant Lythe University of Leeds, UK
12: 10 pm – 12: 35 pm: Modelling Cytotoxic T Lymphocyte mediated killing of target cells Saikrishna Gadhamsetty Utrecht University, Netherlands
12: 35 – 1 pm: Potential of gamma delta T lymphocytes for immunotherapy of cancer S. V. Chiplunkar ACTREC, Navi Mumbia, India
1:00 – 1: 10 pm: PHOTO (in front of the Tata statue)
1:10 – 2: 25 pm: LUNCH
SESSION III Chairperson: Grant Lythe, University of Leeds, UK
2: 30 – 2: 55 pm: T cell receptor-ligand binding: mathematical modeling Carmen Molina-Paris University of Leeds, UK
2:55 – 3:20 pm: Mesoscale kinetics models of immunologic reactions Soumyendu Raha SERC, IISc
3:20 – 3: 45 pm: Macrophage activation - One receptor, two ligands and two pathways B. Ravindran ILS, Bhubaneshwar
3:45 – 4:10 pm: The immunomodulatory role of glycodelin at the feto-maternal interface Anjali Karande Indian Institute of Science, Bangalore, India
4: 10 – 4:30 pm: TEA
SESSION IV Chairperson: S. Vijaya, Indian Institute of Science, Bangalore, India
4:30 – 4:55 pm: A systems perspective of host–pathogen interactions: predicting disease outcome in tuberculosis Nagasuma Chandra Indian Institute of Science, Bangalore, India
4:55 – 5:20 pm: Innate and autoimmune responses induced by chronic human pathogens: lessons from Mycobacteria and Helicobacter pylori Niyaz Ahmed x University of Hyderabad, India 5:20 – 5:45 pm: Modulation of immune effectors by mycobacteria: Notch1 signaling paves the way K. N. Balaji Indian Institute of Science, Bangalore, India
SESSION V Chairperson: Ranga Udaykumar, JNCASR, India
5: 45 – 6:10 pm: Threshold surface density of Gp120-CCR5 complexes necessary for HIV-1 envelope-mediated cell-cell fusion N. Dixit Indian Institute of Science, Bangalore, India
6: 10 – 6:35 pm: Mathematical modeling of anti-retroviral therapy for HIV/AIDS and its impact on controlling the spread of virus in the population Arni S.R. Srinivasa Rao Indian Statistical Institute, Kolkata
6: 35 – 7 pm: The current status of HIV-AIDS in India V. Ravi NIMHANS
7 pm: HIGH TEA
xi
Theoretical and Experimental Immunology
Development and maintenance of the T cell pool in health and disease
Robin Callard Institute of Child Health, London, UK
The peripheral pool of naive cells is established in children over the first 15-20 years of life and then remains fairly constant throughout adulthood. The homeostatic mechanisms that maintain the T cell pool in the face of decreasing thymic output, peripheral cell division and loss by differentiation into memory cells and cell death are now beginning to be understood and can be modelled using ordinary differential equations. This modelling approach has been used to explore the host’s homeostatic response to lymphopenia caused by HIV infection and shows that the slow loss of T cells leading to AIDS in untreated HIV cannot be simply explained by the dynamics of homeostasis. Rather, slow erosion of lymph node architecture required for homeostatic proliferation may explain the gradual decline in the homeostatic set point in HIV as well as the relationship to long term immune reconstitution in response to treatment.
1 Theoretical and Experimental Immunology
Studies on the dynamics of germinal centers
J. Faro University of Vigo Vigo, Spain
Germinal centers (GC) are dynamic, short-lived anatomical structures generated within primary follicles during humoral immune responses. Protein antigens (Ag) are actively transported as immune complexes into B-cell follicles, and ultimately are discharged onto the membrane of the long dendritic arms of so-called follicular dendritic cells (FDCs). After an initial phase, during which activated Ag-specific T and B cells effectively meet at the B cell zone-T cell zone border and interact intensely, some proliferating B cells migrate back toward the center of a follicle together with some T follicular helper (Tfh) cells. This initiates the formation of GCs, which are characterized in the first half of the GC reaction by intense B cell proliferation and increasing apoptosis. Currently, it is well established that memory B cell generation and somatic hypermutation take place in GCs. In addition, accumulated data over the last 15 years clearly indicate that a selection process between Ag-specific B cells, whether antibody-mutated or not, takes place in GCs, accounting at least partially for affinity maturation.
The population dynamics and transient nature of GCs, as well as the mutation-selection processes taking place there, highlight very interesting problems, amenable to analysis by mathematical and computational tools. In this talk I will summarize current key, open questions on GCs as well as some work developed on the question of GC clonal diversity and on possible mechanisms for the rise-and- fall dynamics of the GC reaction.
2 Theoretical and Experimental Immunology
Agent-based simulation of T cell immune response: modelling an adoptive transfer experiment
Gib Bogle1, Rod Dunbar2, Evelyn Hyde3, Franca Ronchese4 1Auckland Bioengineering Institute, University of Auckland; Maurice Wilkins Centre, New Zealand 2School of Biological Sciences, University of Auckland; Maurice Wilkins Centre 3Malaghan Institute 4Malaghan Institute, Maurice Wilkins Centre
Many factors are involved in determining the magnitude of an adaptive immune response. The main reason for developing a simulation model for the T cell immune response in a lymph node is to gain a better understanding of how the various processes act in concert. We are developing an agent- based model that simulates T cell trafficking, motility, interactions with DCs bearing antigen, TCR stimulation, activation and proliferation. This work has revealed many areas where quantitative information is lacking in the literature, and we have begun experiments to fill some of these gaps. Here we report the results of the first experiment, the first attempts to reproduce the experimental results by numerical simulation, and interesting issues that this approach revealed: proliferation outside the LN greatly exceeds that within the LN, and activated CD8+ T cells exit the LN at a much greater rate than naïve non-cognate cells.
3 Theoretical and Experimental Immunology
T cells and ageing: effects of age on survival and function
Vineeta Bal National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110067, India.
Poor T cell immunity is one of the many defects seen in elderly humans and aged mice. While the individual organism ages, most of the senescent cells in her body are periodically replaced over a period of time. Thus liver shows minimal effect of organismal ageing whereas naive T cells show a major compromise in their function. One way to explain the difference is that a simple replacement of the liver cell is possible by division of an adjoining cell whereas that of T and B cells is not because of the somatic recombinations which take place in the T and B lineage cells after which they undergo positive and negative selection. Somatic recombination ability provides for the high repertoire diversity and potential to recognise extremly diverse set of antigens. Thus, analysing consequences of naive T cells which have different T cell receptors and which differ in their individual age becomes an interesting question - different from what happens to other senescent cells in the body.
We have used two approaches to study this issue. In the first approach, we isolate naive CD4 T cells from mice older than 18 months and those 2 months in age and compare their function and survival in vitro. We find that naive CD4 T [NCD4] cells purified from the spleens of aged mice [ANCD4 cells] showed greater apoptosis upon primary activation than those from young mice [YNCD4 cells] early during activation. In the second approach we transfer naive CD4 T cells in congeneic recipient mice and allow donor cells to age for a defined period to analyse the effects. Thus, T cell receptor transgenic [TCR Tg] DO11.10 or OT-2 cells were transferred to congeneic recipients and cells were allowed to age in vivo upto 6 months. This approach provided us with TCR Tg cells which are more homogeneous in age and are clonal in nature. We find that more aged TCR Tg cells proliferate poorly, upregulate CD69 less efficiently and their numbers in vivo also decrease with age, an indication of in vivo apoptosis. Thus prolonged survival in the absence of specific peptide-MHC ligand based activation signal appears to make T cells lose their function.
4 Theoretical and Experimental Immunology
Brownian motion and the adaptive immune system
Grant Lythe University of Leeds, UK.
I will consider simple models of the movement and interaction of T cells and dendritic cells in a lymph node, and analysis of data from in vivo imaging experiments. The adaptive immune response depends on T cells coming into physical contact with antigen-presenting cells (APCs) in the T cell zone of one of a lymph node. Recent advances in imaging techniques, especially two-photon microscopy, have enabled direct observation of the movement of these labelled cells in the lymph nodes of living mice. One increasingly robust conclusion from analysis of the trajectories of individual cells is that they follow random paths. We will think of a lymphocyte as a particle undergoing Brownian motion inside a lymph node. Brownian motion is imagined here as the result of many constantly- changing influences that jostle the cell, without any consistently preferred direction, or due to a preference for moving along a network that is randomly arranged. Timescales are expressed in terms of very few parameters: the diffusivity of a cell, the size of the volume that the cell explores, and the radii of the zone of attraction of an APC.
5 Theoretical and Experimental Immunology
Modelling cytotoxic T lymphocyte mediated killing of target cells
Saikrishna Gadhamsetty1, Joost Beltman1, Vitaly Ganusov2, Sadna Budhu3, Samuel Silverstein3,4 and Rob de Boer1 1Theoretical Biology and Bioinformatics, Utrecht University, Padualaan 8, 3584CH, Utrecht, The Netherlands. 2Department of Microbiology, University of Tennesse, Knoxville TN 37996, USA. 3Department of Physiology and Cellular Biophysics, 4Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
Cytotoxic T Lymphocytes (CTLs) kill virus-infected and tumor cells, and thus play a crucial role during an adaptive immune response. In this study, we analyze a recently published data set of CTL mediated killing of tumor cells in collagen gels, and find that CTLs kill less efficiently at increased concentrations. The decrease in killing efficiency already occurs at very low CTL densities and thus cannot be explained by (direct or indirect) interference between CTLs after they have detected targets. Rather, we find that the limitation must lie in the search efficiency of CTLs. Using a simulation model of T cell migration, we show that a potential explanation for the decrease in search efficiency could be the presence of chemo-attraction by tumor cells.
6 Theoretical and Experimental Immunology
Potential of gamma delta T lymphocytes for immunotherapy of cancer
S. V. Chiplunkar Advanced Centre for Treatment, Research & Education in Cancer, Tata Memorial Centre, Kharghar, Navi Mumbai 410210, India.
Gamma delta T lymphocytes are a distinct subset of lymphocytes that display unique features with respect to T cell receptor gene usage, tissue tropism and antigen recognition. Aminobisphosphonates inhibit the farnesyl pyrophosphate synthase (FPPS) enzyme in a dysregulated mevalonate pathway of tumor cells. This leads to elevated levels of intracellular IPP (isopentenyl pyrophosphate) which are recognised as antigens by Vã9Vδ2 T cells. In the present investigation, attempts have been made to understand the molecular mechanism involved in gamma delta T lymphocyte and tumor cell interaction upon treatment with aminobisphosphonates. Studies demonstrated that aminobisphosphonates sensitize tumor cells to augmented lysis by Vã9Vδ2 T cells. Cell cycle analysis of aminobisphosphonate treated tumor cells revealed a G1/S phase accumulation which may predispose tumor cells to apoptosis. Time lapse video microscopy showed strong conjugate formation between Vã9Vδ2 T cells and tumor cells. Immunonotherapeutic potential of bisphosphonate activated Vã9Vδ2 T cells were evaluated in vivo in mice xenografted with human breast tumors. After adoptive transfer of Vã9Vδ2 T cells increased apoptosis of tumor cells was observed. Vã9Vδ2 T cells also mediate antibody dependant cellular cytotoxicity (ADCC) against tumor cells treated with aminobisphosphonates. Our studies elucidate the dual effects of aminobisphosphonates on Vã9Vδ2 T cells and tumor cells and further establish its potential for immunotherapy of primary tumors and metastasis.
7 Theoretical and Experimental Immunology
T-cell receptor-ligand binding: mathematical modelling
Carmen Molina-Paris University of Leeds, UK.
T-cells sense their environment by means of T-cell receptors (TCRs) on their surface. A T-cell expresses about 30,000 copies of a unique (clonotypic) TCR, whose ligands are complexes composed of a peptide bound to an MHC molecule (pMHC). In vivo, TCR ligands are expressed on the surface of antigen-presenting cells (APCs). In the thymus a variety of professional APCs will subject immature T-cells (or thymocytes) to a “double test” by displaying a wide range of pMHC complexes, with peptides derived from household proteins (self-peptides). The stochastic nature of gene rearrangements implies that some TCRs will not be able to recognise a self-pMHC ligand (TCRs that are not functional) and that others will recognise it far too well, and thus would give rise to mature T-cells with the potential to generate autoimmune responses. Thus, the need for a double test that will check the functionality of a thymocyte (positive selection) and its state of tolerance, so that it does not recognise self-pMHC complexes with high affinities (negative selection). This thymic selection process only allows 2-5% of thymocytes to become mature T-cells.
We have made use of mathematical modelling to address the following issues: (1) the thymic affinity threshold hypothesis proposed by Palmer and Naeher (Nature Reviews Immunology, 2009) and (2) time is precious for T cells, so what do TCRs sense (i) equilibrium properties or (ii) stochastic events. We have made use of data from Palmer’s group (The Journal of Experimental Medicine, 2007) to compare the equilibrium versus the stochastic hypotheses. Our results indicate that the stochastic hypothesis ties in better with the existing immunological evidence and provides support to the affinity threshold hypothesis. The stochastic model has also been applied to recent two-dimensional binding data by Huang et al. (Nature 2010) and sheds light into 2d versus 3d binding kinetics and T-cell responses.
8 Theoretical and Experimental Immunology
Mesoscale kinetics models of immunologic reactions
Soumyendu Raha Supercomputer Education and Research Centre Indian Institute of Science, Bangalore 560012, India.
The talk would describe modelling of meso-scale (i.e., medium sized population of reactant species molecules) biochemical kinetics models of reaction networks of immune systems. Physical and mathematical characterization of these models which are chemical Langevin equations and simulation techniques will be discussed along with examples.
9 Theoretical and Experimental Immunology
Macrophage activation - One receptor, two ligands and two pathways
B. Ravindran Institute of Life Sciences, Bhubaneswar, India.
Innate immunity to pathogenic organisms is mediated by bacterial/viral and fungal products through host Toll Like Receptors that activate macrophages. Endotoxin (LPS) from Gram negative bacteria activates macrophages by engaging TLR4 and other cell surface receptors such as MD2 and CD14 which results in production of inflammatory mediators such as TNF-α, IL-1β, IL-6 etc. Intracellular pathways involved in this process have been understood significantly in recent years. While searching for the elusive innate receptor for nematode pathogens we stumbled on a filarial glycoprotein (designated as AgW) that bound to TLR4. Carbohydrates moieties in AgW were involved in this interaction. But unlike LPS, AgW binding to TLR4 resulted in alternate activation of macrophages, a feature characterised by release of anti-inflammatory mediators such as Arginase 1, Chitinases, IL-10 etc., AgW and LPS competitively inhibited each others’ activation pathways. LPS fails to activate macrophages of C3H/HeJ mice due to a single residue mutation in intracellular domain of TLR4. This mutation also affected alternate activation of macrophages by AgW. This novel feature viz., LPS and AgW activating macrophages by functionally opposite pathways was used to block LPS mediated endotoxemia both in vitro and in vivo. Chitohexaose (chtx), the carbohydrate residue inhibited LPS induced inflammatory molecules and protected mortality of experimental mice suggesting the possibility of using a small molecular weight polysaccharide, chtx as a TLR4 antagonist to block endotoxemia associated with Sepsis, which is responsible for mortality of about 400,000 humans worldwide.
10 Theoretical and Experimental Immunology
The immunomodulatory role of glycodelin at the feto-maternal interface
Anjali Karande Department of Biochemistry, Indian Institute of Science, Bangalore, India
The fetus is an allograft, half paternal, yet, in a normal pregnancy the fetus stays well protected because of several diverse mechanisms which act in a concerted manner to either suppress fetal allogenicity or inhibit the deleterious effects of the maternal immune response. Of the several mechanisms that have been reported so far, one is the immunomodulatory activity of a glycoprotein, named glycodelin. Glycodelin is the most abundant progesterone-regulated secretory glycoprotein of the endometrium at implantation and during early pregnancy in humans and has been proposed as a biochemical marker of endometrial sufficiency. That glycodelin is indispensable for the establishment and progression of pregnancy has been well established and its effects on the various cells of the immune system have been defined to some extent. Glycodelin inhibits the proliferation of activated T-lymphocyte and also induces apoptosis in them; Glycodelin induces apoptosis in monocytic cells before differentiation, but does not affect their phagocytic capacity after differentiation. Glycodelin inhibits B-cell proliferation; Glycodelin inhibits NK cell activity, induces apoptosis in NK cells but the signaling is independent of caspases. In conclusion, although the type of effect of glycodelin and the signaling mechanisms involved in each cell type are distinct, the molecule serves to keep the maternal immune response to the fetal allograft in check.
11 Theoretical and Experimental Immunology
A systems perspective of host–pathogen interactions: predicting disease outcome in tuberculosis
Nagasuma Chandra Department of Biochemistry, Indian Institute of Science, Bangalore - 560 012, India.
The complex web of interactions between the host immune system and the pathogen determines the outcome of any infection. A computational model of this interaction network, which encodes the intricate interplay among host and bacterial components, forms a useful basis for improving the understanding of pathogenesis, in filling knowledge gaps and consequently to identify strategies to counter the disease. A model of the Mycobacterium tuberculosis host–pathogen interactome, built recently in our laboratory, will be presented in this talk. The model contains 75 nodes, about a fourth of them relating to bacterial components, the rest being components of the human immune system. The complex regulation by the various cytokines present in the cell has also been encoded in the model. Boolean transfer functions describe the relationships between the nodes. Vaccination effects, clearance efficiencies due to drugs and growth rates have also been encoded in the model. Virtual deletion experiments were performed, where one or more components of the system were removed and the response of the system to this perturbation was analysed. In these simulations, disabling processes such as phagolysosome fusion or important cytokines such as TNF-α and IFN-γ greatly impaired bacterial clearance, while removing cytokines such as IL-10 alongside bacterial defence proteins such as SapM greatly favoured bacterial clearance. An interesting result from the simulations is the identification of the high propensity of the tubercle bacillus to enter a state of ‘persistence’ under a variety of conditions. The model proposed provides insights into TB pathogenesis and also provides a ready framework for integrating a broad range of experimental information as well as quantitative data at various levels.
12 Theoretical and Experimental Immunology
Innate and autoimmune responses induced by chronic human pathogens: Lessons from myco- bacteria and Helicobacter pylori
Niyaz Ahmed Pathogen Biology Laboratory, Department of Biotechnology, University of Hyderabad, Hyderabad, India.
Several putative virulence encoding genes have been identified in Mycobacterium tuberculosis and Helicobacter pylori that possibly play crucial roles during chronic persistence of these bacteria and provide them with survival advantages. We studied the signaling pathways pertaining to proapoptotic and/or proinflammatory behavior of different proteins from H. pylori and pathogenic mycobacteria. In the former, many of such genes are encoded by the ‘plasticity region cluster’ of the genome and we looked at functions of proteins from this cluster, which are simultaneously proinflammatory and proapoptotic. In M. tuberculosis, we studied a novel member of dos-R family that has both regulatory and innate immune functions. In addition, we identified a few genes/proteins in M. avium subsp. paratuberculosis (that have homologues also encoded by the human genome) that entail potential autoimmune responses; these have significant bearing on our understanding of diseases such as type-1 diabetes mellitus, Crohn’s disease and multiple sclerosis.
13 Theoretical and Experimental Immunology
Modulation of immune effectors by mycobacteria: Notch1 signaling paves the way
K. N. Balaji Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Pathogenic mycobacteria have evolved unique strategies to survive within the hostile environment of macrophages. In this intricate process, modulation of immune effectors like SOCS3, COX-2 and MMP-9 acts as an important factor influencing the overall host immune response. Our study demonstrates that Mycobacterium bovis Bacille Calmette-Guérin (M. bovis BCG) triggered TLR2-dependent signaling leads to induced expression of SOCS3, COX-2 and MMP-9 in macrophages. Signaling perturbations or genetic approaches suggest signaling integration through cross-talk between Notch1, PI3K during M. bovis BCG triggered expression of multitude of immunological parameters including SOCS3, COX-2 and MMP-9. Intriguingly, Nitric Oxide assumes critical importance in M. bovis BCG mediated activation of Notch1 signaling as iNOS-/- macrophages exhibited compromised ability to execute M. bovis BCG triggered Notch1 signaling responses. These findings provide new insights into mechanisms by which Notch1, TLR2 and NO signals are integrated in a cross-talk that modulate defined set of effector functions in macrophages.
14 Theoretical and Experimental Immunology
Threshold surface density of Gp120-CCR5 complexes necessary for HIV-1 envelope-mediated cell-cell fusion
Narendra Dixit Department of Chemical Engineering, Indian Institute of Science, Bangalore 560012, India.
Binding of the HIV-1 envelope (Env) protein gp120 with CCR5 is essential for the entry of R5 viruses into target cells. The threshold surface density of gp120-CCR5 complexes that enables HIV-1 entry remains poorly estimated. We constructed a mathematical model that mimics Env-mediated cell-cell fusion assays, where target CD4+CCR5+ cells are exposed to effector cells expressing Env in the presence of a coreceptor antagonist and the fraction of target cells fused with effector cells is measured. Our model employs a reaction network based approach to describe protein interactions that precede viral entry coupled with the ternary complex model to quantify the allosteric interactions of the coreceptor antagonist and predicts the fraction of target cells fused. By fitting model predictions to previously published data of cell-cell fusion in the presence of the CCR5 antagonist vicriviroc, we estimated the threshold surface density of gp120-CCR5 complexes for cell-cell fusion. Model predictions with this threshold captured data from independent cell-cell fusion assays in the presence of vicriviroc and rapamycin, a drug that modulates CCR5 expression, as well as assays in the presence of maraviroc, another CCR5 antagonist, using sixteen different Envclones derived from transmitted or early founder viruses. Our estimate of the threshold surface density of gp120-CCR5 complexes necessary for HIV-1 entry thus appears robust and may have implications for optimizing treatment with coreceptor antagonists, understanding the non-pathogenic infection of non-human primates, and designing vaccines that suppress the availability of target CD4+CCR5+ cells.
15 Theoretical and Experimental Immunology
Mathematical modeling of anti-retroviral therapy for HIV/AIDS and its impact on controlling the spread of virus in the population
Arni S.R. Srinivasa Rao Indian Statistical Institute, Kolkata, India
After the rapid scale up of anti-retroviral therapy (ART) in India, especially during the third phase of the National AIDS Control Program (NACP III) (2007-12), there is a need to understand the impact of such therapy on both the dynamics of HIV spread and occurrence of new cases. In this analysis the experimental evidence available from various cohorts of individuals who were recruited for ART is blended with dynamical models specifically developed for studying the impact of ART on the population. These models essentially consist of sets of integral-differential equations and a combination of conditional probabilities. ART, in combination with prevention measures, has contributed to the reduction of HIV burden in several countries. In this talk, we present the methods and model developed for tracing the impact of ART in India during the third round of National AIDS Control Program (2007-2012). We highlight the concepts of ‘halting’ and ‘reversal’ of the epidemic and try to build a theory involving these concepts. Projected ART impact and projected HIV during the next phase of the NACP (2012-2017) are discussed. We further studied stability of the system. We have conducted sensitivity analysis of the level of ART intervention on the program results. The analysis led to new results on transition theory pertaining to the concepts of ‘halting’ and ‘reversal’. This talk is based on the joint working paper listed in the reference.
Reference Rao, Arni S.R. Srinivasa, Thomas Kurien, Sudhakar Kurapati, Bhat Ramesh and Maini, P.K. (2011). Working Paper in progress (63 pages). Earlier version of the draft paper was prepared as an input for preparatory process for the Fourth Phase of National AIDS Control Program, NACP-IV (2012-17), National AIDS Control organization (NACO), Department of AIDS Control, Ministry of Health and Family Welfare, Government of India.
16 Theoretical and Experimental Immunology
The current status of HIV-AIDS in India
V. R avi NIMHANS, Bangalore, India
17 Theoretical and Experimental Immunology
LIST OF SPEAKERS
Niyaz Ahmed Edificio de Ciencias Experimentales LS-296, School of Life Sciences, Campus As Lagoas-Marcosende University of Hyderabad, Gachibowli, 36310 Vigo, Spain Hyderabad 500046, India Email: [email protected] Email: [email protected] Saikrishna Gadhamsetty Vineeta Bal Z529, Theoretical Biology and Bioinformatics National Institute of Immunology, Utrecht University, Padualaan 8, 3584 CH Aruna Asaf Ali Marg, Utrecht, The Netherlands New Delhi 110 067, India. Email: [email protected], Email: [email protected] [email protected]
K. N. Balaji Anjali Karande Dept. of Microbiology & Cell Biology Department of Biochemistry Indian Institute of Science Indian Institute of Science Bangalore-560012, India Bangalore-560 012, India Email: [email protected] Email: [email protected]
Gib Bogle Grant Lythe Senior Research Fellow Reader in Applied Mathematics Auckland Bioengineering Institute University of Leeds, Leeds LS29JT, UK University of Auckland, New Zealand Email: [email protected] Email: [email protected] Carmen Molina-Paris Robin Callard Professor in Applied Mathematics Professor, Institute of Child Health University of Leeds, Leeds LS2 9JT, UK University College London Email: [email protected] 30 Guilford st, London WC1N 1EH, UK Soumyendu Raha Nagasuma Chandra SERC Associate Professor Indian Institute of Science, Dept. of Biochemsitry Bangalore - 560 012, India Indian Institute of Science, Email: [email protected] Bangalore - 560012, India E-mail: [email protected], B. Ravindran [email protected] Director, Institute of Life Sciences, (Department of Biotechnology) S. V. Chiplunkar Nalco Square, Bhubaneswar 751023, India ACTREC, Tata Memorial Centre (TMC) Email: [email protected], Sector-22, Kharghar [email protected] Navi Mumbai, 410 210, India Email: [email protected] V. R av i Professor and Head Narendra M. Dixit Dept. of Neurovirology Department of Chemical Engineering NIMHANS, Bangalore – 560029, India Indian Institute of Science, Email: [email protected] Bangalore - 560 012, India Email: [email protected] Arni S.R. Srinivasa Rao B.I.R.U., 4th Floor, R.A. Fisher Building Jose Faro Indian Statistical Institute University of Vigo 203 B.T. Road, Kolkata 700108, India Department of Biochemistry, Email: [email protected] Genetics and Immunology
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LIST OF PARTICIPANTS Outstation Participants for the “International Meeting on Theoretical and Experimental Immunology” on August 16, 2011
1. Akula Srinivas 9. Dollu Srichakra 3-113, Vadapally, Damacherlla 80/112 G 2-A-1, Setlams Nilayam, Nalgunda District, Andhra Subhash Nagar, Kurnool District, Email: [email protected] Andhra Pradesh Email: [email protected] 2. Ashutosh Kumar LS-273, Dept of Biotechnology, 10. G Muni Swamy, M.Sc. University of Hyderabad, Immuno Technology, Hyderabad Dept. of Bio Chemistry, Email: [email protected] Srivenkateswara University, Tirupati Email: [email protected] 3. B Hemanth Nayak Room No. 51, ‘B’ Block, 11. Gandreti Santhosh Krishna Rao Boys Hostel, SV University, Kothaboddam, Boddam Post, Tirupathi, Chittoor District, Vepada Mandal, Viziyanagaram, Andhra Pradesh Andhra Pradesh Email: [email protected] Email: [email protected]
4. Bandari Sreevani 12. Harman Preet Kaur Dhillon H. No. 81/155, Parvathi Nagar, D/o Harjinder Singh Dhillon, Kurnool District, Giri Road, Hillpatna, Berhampur, Andhra Pradesh Dist. Ganjam, Orissa Email: [email protected] Email: [email protected]
5. Bhagchand Gurjar 13. J Cyril Jones V230, Vashistha Hostel, 10/2, Diwan Bhashyam Street, SASTRA University, Thanjavur West saidapet, Chennai Email: [email protected] Email: [email protected]
6. Bhairav Paleja 14. J Sudheer Kumar KS-262, Chiplunkar Lab, Khanolkar H. No: 50-348-M-3-1, Arora Nagar, Shodhika, ACTREC, Tata Memorial B-Camp, Kurnool District, Centre, Kharghar, Navi Mumbai Andhra Pradesh Email: [email protected] Email: [email protected]
7. Byreddy Alekhya 15. K V Khajapeer Door No. 11/37, PTM Road, Door No: 19-4-123/8/B, S.T.V. Nagar, B. Kotha Kota, Chitttoor Disitrict, Tirupathi, Chittoor District, Andhra Pradesh Andhra Pradesh Email: [email protected] Email: [email protected]
8. Dodla Sumathi 16. Kishore Babu Kammapalem Village, 10-1-707, Surendra Nagar Kodavaluru Mandal, Badvel(m), Kadapa, Andhra Pradesh Nellore District, Andhra Pradesh Email: [email protected] Email: [email protected]
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17. Kishore Nalam 25. Praveen Boddana Pathogen Biology Laboratory, C/o S.P.S. Bisht, Roland Institute of Dept of Biotechnology, School of Life Pharmaceutical Sciences, Sciences, University of Hyderabad Berhampur, Orissa Email: [email protected] Email: [email protected]
18. Koustava Kumar Panda 26. Preethi Narayani T R C/o Satpal Singh Bisht, Prof. & AMP; New No. 31, Old No. 9, Subbu Street, Head, Dept. of Biotechnology Thiruvanmiyur, Chennai Roland Institute of Pharmaceutical Email: [email protected] Sciences, Gopalpur Junction, Berhampur, Ganjam, Orissa 27. Pushpa Singh Email: [email protected] D/o A.K. Singh, Qtr No. 1721, H.A.L Township, Ojhar Nasik, 19. Kumar Mohit Maharashtra Prist University Email: [email protected] Email: [email protected] 28. Puttareddy Divyamrita 20. Maitreyee Sharma 4-20, Sri Krishna Nagar, M.R. Palli, C/o H.O.D. Dept. of MBBT, Tirupathi, Chittoor District, Tezpur University, Tezpur, Andhra Pradesh Napaam, Assam [email protected] Email: [email protected] 29. R. K. Anantha Seetha 21. Mohammed Majid 29/3, Sugar Mill Colony, LS-273, Pathogen Biology Lab, Tirunelvelirk Dept. of Biotechnology, Email: [email protected] University of Hyderabad, Hyderabad, Andhra Pradesh 30. Ram Kumar Email: [email protected] 2/404,V.O.C Nagar, Belathur Post, Hosur 22. Pasupuleti Dhanamjayulu Email: [email protected] House No. 8-50/3/a, Before Revands Factory, Chandragiri, Tirupathi, 31. Rubia L asker Chittoor District, Andhra Pradesh Sadar para, Jitu Mistry Lane, Email: [email protected] Purulia (723101), West Bengal Email: [email protected] 23. Poornemaa .N ‘B’ Tulsi Nivas, 21/69, Canal Bank Road 32. S. Venkateswarlu East, R.A. Puram, Chennai House No: 8-17-127/52/1a/1, Email: [email protected] Nallabanda Street, Giddalur Mandal, Prakasam District, Andhra Pradesh 24. Pratheek BM Email: [email protected] School of Biological Sciences National Institute of Science Education & 33. Sangram Keshari Singh Research, Institute of Physics Campus, Raveswaya Park, Gunjan, P.O: Sainik School, Vapi, Gujrarat Bhubaneswar, Orissa Email: [email protected] Email: [email protected]
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34. Sankar. K 42. Valathati Stella Sravanthi B-Block, Room No. 35, Plot No: 24, SV Badra Residency, SVU College of Science, Men Hostels, Balaji Hills Colony, Near Gayatri Gardens, Sri Venkateswara University, Nizampeta Village, Hyderabad, Tirupathi Andhra Pradesh Email: [email protected] Email: [email protected]
35. Santhi 43. Veeram Sindhuja C/o Alamelu Raja, Dept of Immunology, H. No. 15-9H-5-6, Tuberculosis Researchcenter, Government Hospital Road, Chetpet, Chennai Near New Maszid, Nandikotkur, Email: [email protected] Kurnool District, Andhra Pradesh Email: [email protected] 36. Savita Devi Room No: LS-273, 44. Vidya Ranganathan Pathogen Biology Laboratory, Immunobiology Lab I, C/o Vineeta Bal, Department of Biotechnology, National Institute of Immunology, School of Life Sciences, Aruna Asaf Ali Marg, New Delhi University of Hyderabad, Hyderabad Email: [email protected] Email: [email protected] 45. Y Karthik 37. Singam Chaithanya Kumari Door No: 202, B Block, Surya Towers, H. No: 57/19, Chidambara Rao Street, Dharmapet, Kurnool District, Kurnool District, Andhra Pradesh Andhra Pradesh Email: [email protected] E-mail: [email protected]
38. Sneha S 46. Z. Md. Wasimh 139/17 Kailash Colony, Annanagar West No. 8/64c, Maldarpet, Nandyal, Extnsion, Chennai Kurnool District, Andhra Pradesh Email: [email protected] Email: [email protected]
39. Sowmya. R 47. Vani Janakiraman No. 32 Montieth Mansion, Montieth Road, 46c, Shanthi Nagar - West Main Egmore, Chennai Hosur 635109, Krishnagiri Dist, Email: [email protected] Tamil Nadu Email: [email protected] 40. Subramanyam Dasari Research Scholar 48. Harsha. S. N C/o. Prof. W. Rajendra 323, HPO & amp; RMS layout, Dept.Of Zoology, Shakti Nagar, Mysore S.V. University, Tirupati Email: [email protected] Email: [email protected] 49. A Sainath Reddy 41. Tarugu Diwakar Reddy Bijiwar, Maldakal, Mahabuanagar MSc (Biochemistry) Student Email: [email protected] Dept.of Biochemistry, S.V. Universuty, Tirupati 50. Anuradha Email: [email protected] Department of Immunopathology PGIMER, Chandigarh Email: [email protected]
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51. B Subhasini 60. P Bhaskar Godugunuru, Badvel, YSR District, Room No: 51, ‘B’ Block Anajandri, Andhra Pradesh SVU Campus, Tirupathi Email: [email protected] Email: [email protected]
52. G Devika 61. P. Sandhya Rani Om Shanthi Nagar, LS-273, Pathogen Biology Lab, Kadapa District, Andhra Pradesh Department of Biotechnology, Email: [email protected] University of Hyderabad, Hyderabad Email: [email protected] 53. G Lakshmi Devi H. No: 16/48, Kovelakunta, 62. S Mahaboob Basha Kurnool District, Andhra Pradesh 4/73, C.C. Kothakota, [email protected] Pothulangepalli, Dharmavarm, Anatpur 54. G R Bhanu Prakash Email: [email protected] Room No: 51 ‘B’ Block Anjandri, SVU Campus, Tirupathi 63. S Shabeer Email: [email protected] Merugudoddy, Burandoddy, Kodumur, Kurnool District 55. Giri Kumar Email: [email protected] State Bank Lane, Addanki, Prakasam Dist. Email: [email protected] 64. Thandlam Mahesh Kumar Door No: 343, Balaji Nagar, 56. K Narasappa Tirumala, Chittoor District, Room No: 35, B Block Anjanadri, Andhra Pradesh SVU Campus, SV University, Tirupathi Email: [email protected] Email: [email protected] 65. V Varalakshmi 57. K Padmajameenakshi D No : 9/194, K. Bhaskarpet, Gonegandla, Kurnool District, Mylavaram, Kadapa District, Andhra Pradesh Andhra Pradesh Email: [email protected] Email: [email protected]
58. Kanala Vijaya Kumar 66. Bhushan S. Patil D. No: 1-477-1, Santi Nagar, Industrail Biotechnology, Dharmavarm, Anatapur District Sastra University, Thanjavur Email: [email protected] Email: [email protected]
59. Mopuri Ramgopal 67. M. Suvarsha Rao C/o M. Balaji, Dept. of Biochemistry, CCMB, Hyderabad S.V. University, Tirupati Email: [email protected] Email: [email protected]
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LIST OF PARTICIPANTS Local Participants for the " International Meeting on Theoretical and Experimental Immunology" on August 16, 2011
Department of Biochemistry 9. Manoj Bhosale C/o Dr Dipankar Nandi 1. Ajay Kumar Dixit Dept of Biochemistry, C/o Prof. Jayabaskaran Indian Institute of Science, Bangalore Dept. of Biochemistry, Email: [email protected] Indian Institute of Science, Bangalore Email: [email protected] 10. Mohit Arora D.N. Rao Lab, Dept. Of Biochemistry, 2. Arvind A Indian Institute of Science, Bangalore The Lab of P. N. Rangarajan, Email: [email protected] Department of Biochemistry, Indian Institute of Science, Bangalore 11. Monali Manohar Email: [email protected] Prof. Anjali Karande’s Laboratory, Dept. of Biochemistry, 3. B. Chetana Indian Institute of Science, Bangalore C/o Prof. Dipankar Nandi, Email: [email protected] Department of Biochemistry, Indian Institute of Science, Bangalore 12. Mrinmoy Das Email: [email protected] C/o Prof. Dipankar Nandi Dept of Biochemistry, 4. Chaitanya Dende Indian Institute of Science, Bangalore Dept of Biochemistry Email: [email protected] Prof. P.N. Rangarajan, Indian Institute of Science, Bangalore 13. Mukta Deobagkar Email: [email protected] C/o Prof. Dipankar Nandi, Dept of Biochemistry, 5. Divya Bhardwaj Indian Institute of Science, Bangalore C/o Prof Anjali A Karande Email: [email protected] Dept of Biochemistry Indian Institute of Science, Bangalore 14. Nishana M Email: [email protected] C/o Dr Sathees C. Raghavan, Dept. of Biochemistry, 6. Emmanuel Stephen V Indian Institute of Science, Bangalore # 22 LIG, HUDCO Email: [email protected] Udayagiri, Mysore Email: [email protected] 15. Piyush Kumar Singh Anjali Karande’s Lab, 7. Iyappan Dept. of Biochemistry, Department of Biochemistry, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected] 16. Ritu Mishra 8. K. Prashanthi C/o Prof. Anjali Karande, C/o Dr Nagasuma Chandra Dept. of Biotechnology, Dept of Biochemistry Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected] 23 Theoretical and Experimental Immunology
17. Rohit Vashisht 26. Rupa Kumari C/o Prof. Nagasuma Chandra, C/o Sathees C. Raghavan, Department of Biochemistry, Dept. of Biochemistry, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
18. Rubeena. M 27. Rajkumar .D Dipankar Nandi’s Lab, 28/10, 2nd Main, Attigupee, Department of Biochemistry, Vijayanagar, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
19. Shwetank Dept. of Biochemistry, Indian Institute of Science, Bangalore Department of Microbiology and Email: [email protected] Cell Biology
20. Soma Das 1. Akhauri Yash Sinha Post Doctoral Fellow, K.N Balaji Lab, Dept of MCBL, C/o Prof. Anjali A. Karande, Indian Institute of Science, Bangalore Dept. of Biochemistry, Email: [email protected] Indian Institute of Science, Bangalore Email: [email protected] 2. Anish R V C/o Dipshika Chakravortty, 21. Srabanti Rakshit CIDR, MCBL, Department of Biochemistry, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected] 3. Debalina Chaudhuri 22. Sudarshan C/o Dipshikha Chakrovortty, C/o Prof. Anjali Karande, Dept of Microbiology and Cell Biology, Department of Biochemistry, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected] 4. Devram Sampat Ghorpade 23. Tanushree C/o K. N. Balaji, Dept. of Microbiology C/o Prof. Dipankar Nandi, and Cell Microbiology, Dept. of Biochemistry, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected] 5. Jamma Trinath 24. Bhagawat Chandrasekar Laboratory of K.N. Balaji, C/o Prof. Dipankar Nandi, Department of Microbiology and Department of Biochemistry, Cell Biology, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
25. Rananaware Supriya Rajendra 6. Kannan B N Department of Biochemistry, C/o Prof. Parag Sadhale, Indian Institute of Science, Bangalore Dept of Micorbiology and Cell Biology, Email: [email protected] Indian Institute of Science, Bangalore Email: [email protected]
24 Theoretical and Experimental Immunology
7. Kavya Ananthaswamy 15. Sahana Holla FACS Facility, Ground Floor, C/o K. N. Balaji, 208, CIDR Building, Dept. of Microbiology Dept. of Microbiology and Cell Biology and Cell Biology, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalorea Email: [email protected] Email: [email protected] 16. Sai Rama Krishna Meka 8. Madhura Joglekar C/o Dipshika Chakraborthy, C/o Dr Dipshikha Chakravortty, Molecular Pathogenesis Lab, Dept of MCB, Indian Institute of Science, Dept. of Micro Biology & Cell Biology, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
9. Namrata Iyer 17. Sandhya C/o Dipshikha Chakravortty, C/o Dipshikha Chakravortty, Department of Microbiology and Dept. of Micro Biology & Cell Biology, Cell Biology, Indian Institute of Science, Indian Institute of Science, Bangalore Bangalore Email: [email protected] Email: [email protected] 18. Sangeeta 10. Pallavi Kakade C/o Dr Dipshikha Room No. 115, Dept of Microbiology and Dept. of Micro Biology and Cell Biology, Indian Institute of Science, Cell Biology (CIDR), Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
11. Prasanna Bhat 19. Sanjay Kumar. P Lab No. 252, Dept of Microbiology and Subba Rao Lab, Microbiology and Cell Cell Biology, Indian Institute of Science, Biology, Indian Institute of Science, Bangalore Bangalore Email: [email protected] Email: [email protected]
12. Preeti Garai 20. Saurabh Kumar C/o Dipshikha Chakrvortty C/o Prof. S Vijaya, Room No 254, Dept. of Microbiology and Cell Biology, Dept of Microbiology and Cell Biology, Indian Institute of Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
13. Puja Pai 21. Shakuntala Pillai FACS Facility, Dept. of Microbiology and C/o K.N. Balaji, Dept. of Microbiology Cell Biology, Indian Institute of Science, and cell Biology, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
14. Ravinayak Pathlavath 22. Shambhuprasad. T.K. C/o K.N. Balaji, Dept. of Microbiology C/o K.N. Balaji Lab, Dept. of and Cell Biology, Indian Institute of Microbiology and cell Biology, Science, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
25 Theoretical and Experimental Immunology
5. Awanti Sambarey 23. Shwetha C/o Dr Nagasuma Chandra, Prof. Saumitra Das’s Lab Indian Institute of Science, Bangalore Dept. of Microbiology and cell Biology, Email: [email protected] Indian Institute of Science, Bangalore Email: [email protected] 6. Sannula Kesavardana C/o Prof. Raghavan Varadarajan’s Lab, 24. Shobha Molecular Biophysics Unit, EB-650, New Housing Colony, Indian Institute of Science, Bangalore Indian Institute Of Science, Bangalore Email: [email protected] Email: [email protected] 7. Sumanta Mukherjee 25. Thomas Paulraj Dept of Mathematics, (IMI office) C/o K.N. Balaji, Dept. of Indian Institute of Science, Microbiology and cell Biology, Bangalore Indian Institute of Science, Bangalore Email: [email protected] Email: [email protected]
26. Divya Prakash G C/o Dr. Dipshikha Chakravortty, IISc Students Centre for Infectious Disease Research (CIDR), Indian Institute of Science, 1. Aparna Bangalore C/o Dr. Upendra Nongthomba, Email: [email protected] Dept. of MRDG, Indian Institute of Science, Bangalore Email: [email protected]
2. Arunita Chatterjee Molecular Biophysics Unit C/o Dr. Upendra Nongthomba, Dept. of MRDG, Indian Institute of 1. Kirtimaan Syal Science, Bangalore Prof. D. Chatterji Lab, Email: [email protected] Molecular Biophysics Unit, Indian Institute of Science, Bangalore 3. Shalu Verma-Kumar Email: [email protected] Centre for Ecological Sciences, Indian Institute of Science, 2. Subho Ghosh Bangalore-560012 & Molecular Biophysics Unit, C/o Dr. K.N. Balaji, Microbiology and Indian Institute of Science, Bangalore Cell Biology, Indian Institute of Science, Email: [email protected] Bangalore Email: [email protected] 3. V Vamsee Aditya Mallajosyula Lab # 202, Molecular Biophysics Unit, 4. Anurag Misra Indian Institute of Science, Bangalore Dept. of Physics, Indian Institute of Email: [email protected] Science, Bangalore Email: [email protected] 4. Priyanka Baloni C/o Prof. Nagasuma Chandra 5. Vidya Devi Bioinfromatics centre, Raman building, SA22 Aryabhatta Aptt. Indian Institute of Indian Institute of Science, Bangalore Science Campus, Bangalore Email: [email protected] Email: [email protected]
26 Theoretical and Experimental Immunology
6. Shiva Naresh 3. Smitha Sharma Lab-1, Dept. of Chemical Engineering, Samarth Life Sciences, #41-p3, Indian Institute of Science, Bangalore 3rd Main Road, 1st Phase Peenya Email: [email protected] Industrial Area, Bangalore Email: [email protected] 7. Supriya Vartak LRG3 Rohini Hostel, 4. Mahima Arora Indian Institute of Science Campus, #1/1, M. Appanna Mansion, Bangalore Kamala Kuteera Girls, PG, Email: [email protected] Thavarekere Main Road, Near Federal Bank ATM, 8. Saswati Dana Thavarekere, Bangalore #18, Paresh Apartments, 4th Main, Email: [email protected] 13th Cross, Malleswaram, Bangalore 5. Abhishek K. Srivastava Email: [email protected] Syngene International Ltd, Biocon Park, BBRC Build. S11, 9. Ruchi Agrawal Plot 2&3, Jigani Link Road, MRDG, Indian Institute of Science, Bommasandra Phase IV, Bangalore Bangalore Email: [email protected] Email: [email protected] 6. Swarupa Yalla 10. Ruchi Jain MBGU, JNCASR, Jakkur Post, MRDG, Indian Institute of Science, Bangalore Bangalore Email: [email protected] Email: [email protected] 7. Prabhu S A 11. Raji Nair HIV - AIDS Laboratory, MRDG, Indian Institute of Science, MBGU, JNCASR, Jakkur P.O., Bangalore Bangalore Email: [email protected] Email: [email protected]
12. Mayur Rajankar 8. Shilpee MRDG, Indian Institute of Science, HIV-AIDS Lab, MBGU, JNCASR Bangalore Jakkur, Bangalore Email: [email protected] Email: [email protected]
9. Supriya. R #74, 3rd Main, Nagendra Block, Local Participants from Bangalore SBM Colony, Bangalore Email: [email protected] 1. Vikas Agarwal Srinivas 2nd Cross, 2nd Main, 10. Meenakshi P Hulimavu Gate, Thimmappa Reddy B1, Teresa Towers, 10th ‘A’ Cross, Layout, Bannerghatta Road, 16th Main, Near Mico Layout, Bangalore Police Station, BTM Layout, Email: [email protected] 2nd Stage Bangalore Email: [email protected] 2. Shivayogi MS Smarth Biologicals Pvt. Ltd. 11. Setareh farzanehkari Main Peenya Industrial Area, 168/21 4th Cross, SG Payla, First Phase, Bangalore Hosur Road, Banglore Email: [email protected] Email: [email protected] 27 Theoretical and Experimental Immunology
12. Vignesh Subramanian 17. K.S. Shabnam #101, Jayalakshmi Apartments, #1096 7th ‘A’ Main, BTM Layout Krishna Reddy Colony, 1st Stage Bannergatta Cross Road, Domlur Layout, Bangalore Bangalore Email: [email protected] Email: [email protected]
13. Pranav R 18. Shailesh Dudhgaonkar Kottakkal Arya Vaidya Sala, S11, BBRC, Biocon Park, 120/3, 6th ‘C’ Main, 30th Cross, Jigni-Link Road, 4th, Block, Jayanagar, Bangalore Bommasandra Industreal area, Email: [email protected] Bangalore Email: [email protected] 14. Princy Khurana Maria Bhavan PG for Ladies, 19. Abhishek. J #89/7, 1st Main, Thavarekere Main Road, No. 1281, 6th Main, 1st ‘A’ Block, S. Gpalya, D.R.C Post, Opposite oracle, 2nd Stage, Rajajinagar, Bangalore Bangalore Email: [email protected] Email: [email protected] 20. Anjali Verma 15. Atmeeya HIV-AIDS Lab, MBGU, JNCASR #117,Vakil Garden City, Bangalore Off Kanakpura Road, Email: [email protected] Near Thallaghattapura, Bangalore Email: [email protected] 21. Mahesh Kumar M C/o. R.S Jayshree, Prof. and Head, 16. Asha Soman Department of Microbiology, # 442, IIM Bangalore, Kidwai Memorial Institute of Oncology, Bannerghatta Road, Hosur road, Bangalore Bangalore Email: [email protected] Email: [email protected]
28 Theoretical and Experimental Immunology
LIST OF HONORED GUESTS
G. Padmanaban Ganesh Nagaraju Department of Biochemistry Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] [email protected]
H. S. Savithri A. Jagannadha Rao Department of Biochemistry Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] [email protected]
D. Narasimha Rao Appaji Rao Department of Biochemistry Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] S. K. Poddar K. Muniyappa Department of Biochemistry Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] T. Ramasarma Department of Biochemistry C. Jayabaskaran Indian Institute of Science Department of Biochemistry Indian Institute of Science R. Maheshwari [email protected] Department of Biochemistry Indian Institute of Science Ram Rajasekharan Department of Biochemistry P. G. Vatsala Indian Institute of Science Department of Biochemistry [email protected] Indian Institute of Science [email protected] P. N. Rangarajan Department of Biochemistry K. P. Gopinathan Indian Institute of Science Department of Microbiology and [email protected] Cell Biology Indian Institute of Science Utpal S. Tatu [email protected] Department of Biochemistry Indian Institute of Science H. G. Sharat Chandra [email protected] Department of Microbiology and Cell Biology Shikha Laloraya Indian Institute of Science Department of Biochemistry [email protected] Indian Institute of Science [email protected] M. S. Shaila Department of Microbiology and Patrick D' Silva Cell Biology Department of Biochemistry Indian Institute of Science Indian Institute of Science [email protected] [email protected]
29 Theoretical and Experimental Immunology
V. Nagaraja P. Ajitkumar Department of Microbiology and Department of Microbiology and Cell Biology, Indian Institute of Science Cell Biology [email protected] Indian Institute of Science [email protected] Umesh Varshney Department of Microbiology and S. S. Indi Cell Biology Department of Microbiology and Indian Institute of Science Cell Biology [email protected] Indian Institute of Science [email protected] C. Durga Rao Department of Microbiology and Mrinal Ghosh Cell Biology Department of Mathematics Indian Institute of Science Indian Institute of Science [email protected] [email protected]
Usha Vijayraghavan S. Umapathy Department of Microbiology and Department of Inorganic and Cell Biology Physical Chemistry Indian Institute of Science Indian Institute of Science [email protected] [email protected]
Utpal Nath P. Balaram Department of Microbiology and Molecular Biophysics Unit Cell Biology Indian Institute of Science Indian Institute of Science [email protected] [email protected] M. Vijayan K. Somasundaram Molecular Biophysics Unit Department of Microbiology and Indian Institute of Science Cell Biology [email protected] Indian Institute of Science [email protected] Dipankar Chatterji Molecular Biophysics Unit G. Subba Rao Indian Institute of Science Department of Microbiology and [email protected] Cell Biology Indian Institute of Science A. Surolia [email protected] Molecular Biophysics Unit Indian Institute of Science Saumitra Das [email protected] Department of Microbiology and Cell Biology S. K. Sikdar Indian Institute of Science Molecular Biophysics Unit [email protected] Indian Institute of Science [email protected] S. Vijaya Department of Microbiology and Raghavan Varadarajan Cell Biology Molecular Biophysics Unit Indian Institute of Science Indian Institute of Science [email protected] [email protected]
30 Theoretical and Experimental Immunology
N. Srinivasan Paturu Kondaiah Molecular Biophysics Unit Department of Molecular Reproduction, Indian Institute of Science Development & Genetics [email protected] Indian Institute of Science [email protected] B. Gopal Molecular Biophysics Unit Rajan R. Dighe Indian Institute of Science Department of Molecular Reproduction, [email protected] Development & Genetics Indian Institute of Science Vidyanand Nanjundiah [email protected] Department of Molecular Reproduction, Development & Genetics Annapoorni Rangarajan Indian Institute of Science Department of Molecular Reproduction, [email protected] Development & Genetics Indian Institute of Science S. Mahadevan [email protected] Department of Molecular Reproduction, Development & Genetics Vijayalakshmi Ravindranath Indian Institute of Science Centre for Neuroscience [email protected] Indian Institute of Science [email protected] Upendra Nongthomba Department of Molecular Reproduction, Aditya Murthy Development & Genetics Centre for Neuroscience Indian Institute of Science Indian Institute of Science [email protected] [email protected]
Arun Kumar Shyamala Mani Department of Molecular Reproduction, Centre for Neuroscience Development & Genetics Indian Institute of Science Indian Institute of Science [email protected] [email protected] K.R. Shivakumar P. B. Seshagiri Central Animal Facility Department of Molecular Reproduction, Indian Institute of Science Development & Genetics [email protected] Indian Institute of Science [email protected] Rosa Samuel Central Animal Facility R.M. Medhamurthy Indian Institute of Science Department of Molecular Reproduction, [email protected] Development & Genetics Indian Institute of Science Omana Joy [email protected] FACS Facility [email protected] Sandhya S. Visweswariah Department of Molecular Reproduction, R. Sukumar Development & Genetics Centre for Ecological Sciences Indian Institute of Science Indian Institute of Science [email protected] [email protected]
31 Theoretical and Experimental Immunology
Praveen Karanth Anuranjan Anand Centre for Ecological Sciences Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Renee M. Borges Tapas K. Kundu Centre for Ecological Sciences Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Rohini Balakrishnan Kaustuv Sanyal Centre for Ecological Sciences Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Sushama Yermal Namita Surolia UG Program Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Sanjukta Chatterji Hemalatha Balaram UG Program Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Kayvan Zainabadi Maneesha Inamdar UG Program Molecular Biology & Genetics Indian Institute of Science JNCASR [email protected] [email protected]
Rabindranath Nayak Ravi Manjithaya School of Biological Sciences (NISER) Molecular Biology & Genetics Bhubaneshwar JNCASR [email protected] [email protected]
Prof. Subba Rao Jayachandra A N Bigtech Labs Administrative Officer Bangalore JNCASR [email protected] Apurva Sarin Dean Chiranjib Bhattacharyya NCBS Computer Science and Automation, [email protected] Indian Institute of Science [email protected] M. R. S. Rao President Siddhartha Gadgil JNCASR Department of Mathematics [email protected] Indian Institute of Science [email protected] Chandrabhas Narayana Dean JNCASR [email protected] 32 Theoretical and Experimental Immunology
G. K. Anantha Suresh A. M. Umarji Mechanical Engineering Material Research Centre, Indian Institute of Science Indian Institute of Science [email protected] [email protected]
Debnath Pal Bikramjit Basu SERC, Indian Institute of Science Material Research Centre, [email protected] Indian Institute of Science [email protected] Vijay Natarajan Computer Science and Automation Satyajit Mayor Indian Institute of Science NCBS, Bangalore [email protected] [email protected]
A. K. Nandakumaran P. S. Anil Kumar Department of Mathematics, Department of Physics, Indian Institute of Science Indian Institute of Science [email protected] [email protected]
A. G. Menon Chairman Dept. of Instrumentation CSIC Indian Institute of Science Indian Institute of Science [email protected]
Prabal K Maiti Department of Physics, Indian Institute of Science [email protected]
33 Theoretical and Experimental Immunology
LIST OF SPONSORS
1. Jawaharlal Centre for Advnaced 10. Takara / Clonetech Scientific Research (JNCASR) DSS Imagetech Pvt. Ltd. Jakkur P.O., Bangalore - 64. New Delhi Tel: 09871130106 2. Centre for Scientific & Industrial Email: [email protected] Consultancy (CSIC), IISc, Bangalore 11. Laben #599/4, Shop No. 3, 3. Beckman Coulter 2nd Main Road, Prakash Nagar, No. 38/1, 3rd Floor Manasa Bldg, Bangalore - 560 021 4th & 5th Main, 8th Cross, Tel: 9845089463 Malleshwaram, Bangalore - 03 Email: [email protected] Tel: 9900252639 Email: [email protected] 12. Olympus DSS Imagetech Pvt. Ltd. 4. Lead Instruments 245, Raheja Arcade, #20/4 New Bamboo Bazaar Road Second Floor, 1/1, Bangalore - 560 051 Koramangala Industrial Layout Tel: 080-25543954 Bangalore - 560095 Email: [email protected] Tel: 9845294187 Email: [email protected] 5. Care Biomedical #137, Canara Bank Colony, 13. Becton Dickenson Nagarbhavi Road, Bangalore - 560 072 #9 & 10, First Main, Tel: 9448129098 Pampa Extn. Email: [email protected] Hebbal Kempapura, Bangalore - 560 024 6. J. J. Biotek Tel: 9538999875 #36, 5th Cross, 9th Main Email: [email protected] K. N. Extension, Yeshwantpur Bangalore - 560 021 14. Imgenex India Tel: 9448133739 E-5, Infocity, KIIT Post Office Email: [email protected] Bhubaneshwar - 751 024 Tel: (0674) 2743265 7. Inkarp Instruments Email: [email protected] #29 "Adigiri", 1st Cross, Micro Layout, Mahalaxmipuram, 15. Millipore India Pvt. Ltd Bangalore - 560 086 50A, 2nd Phase, Ring Road Tel: 9844021067 Peenya, Bangalore - 560 058 Email: [email protected] Tel: 080-3922 4000 Email: [email protected] 8. Microphil (India) No. U24, 3rd Cross, Pipeline, 16. SISCO Research Laboratories Malleswaram, Bangalore - 560003 26, Navketan Ind., Premises Tel: 9628817363 Shanti Nagar, Mahakali Caves Road, Email: [email protected] Andheri (E), Mumbai - 93 Tel: 022-426805800 9. Biogene India Email: [email protected] 208, Second Floor, Magnum House I Karampura Commercial Complex New Delhi - 110015 Tel: 011-25920047 Email: [email protected] 34 With Best Compliments from: Lab Engineers (India) 599/4, # 3, 2nd Main, Prakash Nagar, (Opp Navrang Restaurant, Dr. Rajkumar Road), Bangalore – 560 021. Ph.: Off: +91 80 23425380 / 23324736 Mob: 9845089463; E-mail: [email protected], [email protected]
Contact Person: Mr. Ajay Chadha Our Specialities are: Manufacturer & Trinocular / Binocular Microscopes Suppliers of High Inverted Tissue Culture Microscopes Precision Metallurgical Microscopes Stereoscopic Trinocular / Binocular Microscopes Microscopes, Phase Contrast, Dark Field, Fluorescence Microscopes,
We also provide custom designed solutions in Microscopy as per Customer's requirement.
LEADINSTRUMENTS
We are the manufacturers of basic laboratory Instruments. Established in the year 1994, we have a vast experience in the field of science and technology.
OUR RANGE OF PRODUCTS INCLUDE: Micro centrifuges Shakers Tube mixers Magnetic stirrers PCR work stations BOD Freezers Incubators Water baths Hot air oven Rockers Bottle roller
You can contact us at the following address
LEAD INSTRUMENTS PVT. LTD. No. 20/4, NEW BAMBOO BAZAAR ROAD, BANGALORE - 560 051 PH: 080 255 44852 / 255 43 954; FAX: 080 255 42 271 MOB: 9845033359; EMAIL: [email protected] Web: www.leadinstruments.in Notes Notes Notes Notes Notes Notes Notes Notes Notes Notes Notes No. 36, 5th Cross, 9th Main, K.N. Extension, Yeswanthpur, Bangalore - 560 022. Telefax: +91 80 23371359 Email: [email protected]
JJ Biotek Product Profile
SANYO – JAPAN 152’C Deep Freezer, -86’C Deep Freezer, -40’C Deep Freezer, -20’C Deep Freezer, Pharmaceutical Refrigerators, Cold Chambers, Pharmaceutical Refrigerators with Freezer, Co2 Incubators, Incubators, Cooled Incubators, Plant growth chambers, Hot Air Oven, Dry Heat Sterilizers, Auto Clave, Blood Bank Refrigerators. Bio safety Cabinets and Data logging systems
QIAGEN – GERMANY Genomic DNA Preparation, Plasmid DNA Purification, DNA Cleanup and Normalization, RNA Sample Technologies, RNAi Solutions, PCR Technologies, Protein Technologies, Transfection Technologies, Automated Solutions, Real Time PCR, QIA Cube, QIAgility, QIAsymphony, EZI Advanced, QIAxtractor
N-BIOTEK – KOREA Live Cell Engineering Station, DNA Vacuum Concentrators, Gas Blowing Station, IR Concentrators, Roller/Shakers, Incubator, Shaker Incubator, Refrigerated Incubator Shaker, Desktop Shaking Incubator, Hybridization Oven, BIO Work Station, PCR Work Station Bio-Safety Cabinet, Vortex Mixer, Dry Bath, Drying Oven, Hot Plate Stirrer
TECHNE – UK Gradient PCR Machine, Non-Gradient PCR Machine, Hybridization Oven, Gelation Timer, Sample Concentrator, Biological Stirrer, Sample cooling, Dri-Blocks, Sample cooling, Thermo regulators
BIOGENICS – USA Programmable Controlled Rate Freezers, Liquid Nitrogen Container, Cryostorage and Transport Instruments, Stage and Specimen Warmers, Portable Incubators, Cell Fusion
SYNGENE – UK Over 60,000 units of Gel Documentation System Installation all over the world. Fluorescence Gel Documentation System, Chemiluminescence and Fluorescence Gel Documentation System, Chemiluminescence Gel Documentation System, Proteomics and TLC Imaging System, 2D Imaging work station
HETTICH – GERMANY Micro Centrifuge, Table Top Micro Refrigerated Centrifuge, Table Top High Speed Refrigerated Centrifuge, Large Volume Refrigerated Centrifuge, Hematocrit Centrifuge, Automatic Cell Washing System.
JJ BIOTEK – INDIA One stop and turnkey solution for lab set up for STEM CELLS, NANO TECHOLOGY and CRO labs from Industries experts. We do service for 3rd party instruments and that is where JJ Biotek strength is. We offer AMC, Calibration for all kind of instruments.
Please contact for Sales : Mr. Johnson + 91 98809 33838 and Mr.Prakash Prabhu + 91 94481 33739 Please contact for Service : Mr.Janardhan + 91 98800 77717 Email ID : [email protected]