© 2017 Palawan Council for Sustainable Development
OUR PALAWAN
The Scientific Journal of the Palawan Council for Sustainable Development
Volume 3 Issue 1, January - June 2017
Published by
The Palawan Council for Sustainable Development (PCSD)
PCSD Building, Sports Complex Road,
Brgy. Sta. Monica Heights,
Puerto Princesa City
P.O. Box 45 PPC 5300 Philippines
PCSD Publications © Copyright 2017
ISSN: 2423-222X
Online: www.pkp.pcsd.gov.ph www.pcsd.gov.ph
Cover Photo
The endemic species of Palawan and Philippines (from top to bottom) : Medinilla sp.,
Palawan Pangolin Manus culionensis spp., Palawan Bearcat Arctictis binturong whitei, Palawan Hill Mynah Gracula religiosa palawanensis, Blue-naped parrot T a nygnathus lucionensis, Philippine Cockatoo Cacatua haematuropydgia. (Photo courtesy: PCSDS)
© 2017 Palawan Council for Sustainable Development
EDITORS’ NOTE
Our Palawan is an Open Access journal. It is made freely available for researchers, students, and readers from private and government sectors that are interested in the sustainable management, protection and conservation of the natural resources of the Province of Palawan. It is accessible online through the websites of Palawan Council for Sustainable Development (pcsd.gov.ph) and Palawan Knowledge Platform for Biodiversity and Sustainable Development (pkp.pcsd.gov.ph). Hard copies are also available in the PCSD Library and are distributed to the partner government agencies and academic institutions. The authors and readers can read, download, copy, distribute, print, search, or link to the full texts of published articles.
Our Palawan practices a double-blind peer review. The review process has basically three stages: (1) primary checking (the Editors make sure that the manuscript complies with the Author’s Guidelines); (2) review by the external reviewers, involving an assessment of its suitability for publication (compatibility with Our Palawan ’ s aims and scopes, evidence of research, sufficiency of references to the international literature, and scientific soundness); and (3) proofreading and checking whether the article is written in compliance with the Author’s Guidelines.
Submission of Manuscript. For the next issue, submission of manuscripts is now open for acceptance. Manuscripts should comply with the Author’s Guidelines which could be obtained by e-mail from the Editors at [email protected], PCSD Building, Sta. Monica Puerto Princesa City, Palawan.
Copyright. The articles for publication should not violate the other author’s rights and copyright, fully or partially, through plagiarism (including self-plagiarism) or inclusion of elements (such as figures and photos) that do not belong to the author and for which no written consent to use is given by the owner. The author ensures that the paper has not been published or submitted for publication to any other journal and that the research described in the paper is original. The author also assumes full responsibility for the contents, correctness and originality of the paper.
© 2017 Palawan Council for Sustainable Development
i
Editorial Board
Nelson P. Devanadera, Executive Director
Atty. Adelina B. Benavente-Villena, Deputy Executive Director
Josephine S. Matulac, Director II
Technical Editors
Engr. Madrono P. Cabrestante Jr., Division Head, EMKM
Marianne Faith G. Martinico-Perez, Research Analyst, EMKKM
Editorial Staff
Arnica D. Mortillero
Kit Jasper S. Hernandez
Celso S. Quiling Jenevieve P. Hara
ISSN: 2423-222X
©Palawan Council for Sustainable Development
PCSD Building, Sports Complex Road, Sta. Monica Heights
Puerto Princesa City, Palawan, 5300, Philippines
ii
© 2017 Palawan Council for Sustainable Development
OUR PALAWAN
The Scientific Journal of the Palawan Council Sustainable Development
Volume 3 Issue 1 January – June 2017
Directory of External Reviewers
Dr. William Sm. Gruezo
Retired Faculty, University of the Philippines Los Baños
Dr. Chona Camile Vince Cruz-Abeledo
De La Salle University
Dr. Allaine T. Baaco
Western Philippines University
Engr. Glenn S. Banaguas OYS
De La Salle Araneta University
Dr. Perry S. Ong
University of the Philippines Diliman
Dr. rer. net. Thomas Edison E. Dela Cruz
University of Sto. Tomas
Dr. Roger G. Dolorosa
Western Philippines University
Dr. Benjamin J. Gonzales
Western Philippines University
Dr. Cherry Myo Lwin
Ritsumeikan University, Japan
Dr. Patrick A. Regoniel
Palawan State University
Dr. Shiela G. Vergara
ASEAN Centre for Biodiversity
Dr. Irina Safitri Zen
Institut Sultan Iskandar (ISI)
iii
© 2017 Palawan Council for Sustainable Development
Palawan Council for Sustainable Development Staff
EXECUTIVE DIRECTOR’S MESSAGE
The publication of Our Palawan is another strategy to provide venue where we can share information that could benefit our students, researchers, communities and stakeholders. This is our humble contribution as environmental research is identified as a support mechanism of the Republic Act 7611. As we proudly claim, we take Palawan Province as a unique living laboratory having the large proportion of endemic plants and animals with total of 32 identified Key Biodiversity Areas (KBAs).
We believe that all information generated through various researches and studies should be shared best in an accessible and user-friendly medium. On this note, we simultaneously upload this publication in digital format through the Palawan Knowledge Platform (PKP). The PKP, on the other hand, is a joint initiative of our organization in cooperation with our partners from various institutions, particularly the academe, other government agencies and the public sector to facilitate information sharing among the member-agencies. We are very pleased that this commendable initiative has gained ground with the active support and cooperation from our partners.
With respect to the policy formulation process, the Palawan Council for Sustainable
Development - Scientific Advisory Panel (PCSD- SAP) has been reactivated to provide expert’s advice on certain issues for resolution of the Council. We endeavor to regularly convene the Panel and be able to seek their inputs on matters that require scientific expertise or opinion. This is our commitment to a science-based decision making by the Council.
The 4th edition of Our Palawan highlights some of the endemic species of Palawan as included in the research on extracts of selected plants in Puerto Princesa City. We also publish the ethno-food study and protein analysis of beans common among the locals called “lumabeng”. In doing so, we attempt to educate our readers of the beneficial components of some of the plants that maybe found just around Puerto Princesa City and the province of Palawan. On the same context, we packaged the information materials and reports presented in the international fora and conferences participated by the Palawan Council for Sustainable Development representing Palawan Biosphere Reserve under UNESCO’s Man and Biosphere Program.
We encourage the students and researchers to continue exploring and then publish the results of their studies. Keep in mind that a single drop of idea today may create a ripple of brilliant thoughts for something big tomorrow!
NELSON P. DEVANADERA
Executive Director
iv
© 2017 Palawan Council for Sustainable Development
Table of Contents
- Editor’s Note
- i
- Executive Director’s Message
- iv
Research Papers
- 1
- Hemagglutination and Antimicrobial Potential of Leaf Crude Extracts of Selected Plants
in Puerto Princesa City, Palawan, Philippines Jhonamie Mabuhay-Omar, Shellajean M. Omar, Julius N. Burgos, Ciara Patricia Dicar, Dan Clark S. Bacani, and Edziel P. Hubo
- 12
- Ethno-Food Study and Protein Analysis of Lumabeng Beans (Vigna umbellata (Thunb.) Ohwi
& H. Ohashi) from Agutaya Island, Palawan, Philippines Ronald S. Brillantes
Feature Articles
19 28 33
Expert Advice from the Scientific Advisory Panel of PCSDS: Output of the 3rd Meeting on 20 June 2017
Facing the Odds: Palawan Battles against Forest Destruction (An Update from the Palawan Biosphere Reserve Periodic Report 2015)
Managing Palawan Biosphere Reserve’s Wildlife and their Habitats (An Update from the Palawan Biosphere Reserve Periodic Report 2015)
Forum/ Conference Papers
47 52
A Review of Palawan’s Coral Reefs Carlos Alfredo A. Castillo
The Zero Carbon Resorts for Sustainable Tourism Project: Promoting Biodiversity Friendly Enterprise
56 60
Research bits About the Authors
v
© 2017 Palawan Council for Sustainable Development
Tubbataha Reefs and Natural Park
declared as Particularly Sensitive Sea Area (PSSN)
Photo Credits: PCSDS
e Tubbataha Refs and Natural Park was approved in principle as a Particularly Sensitive Sea Area (PSSN) by the International Maritime Organisation - Marine Environment Protection Committee (MEPC). e application was approved during MEPC’s 69th session held last 18-22 April 2016 in London. is will serve as an additional protection to the park from shipping vessels that cross its borders which may aground or damage reefs.
vi
© 2017 Palawan Council for Sustainable Development
Our Palawan
The Scientific Journal of the Palawan Council for Sustainable Development
Research Paper
This article is also available on-line at www.pkp.pcsd.gov.ph
Hemagglutination and Antibacterial Potential of Leaf Crude Extracts of Selected Plants in Puerto Princesa City, Palawan, Philippines
Jhonamie Mabuhay-Omar1, Shellajean M. Omar2, Julius N. Burgos1,
Ciara Patricia Dicar1, Dan Clark S. Bacani1, and Edziel P. Hubo1
ABSTRACT
This study aimed to screen the presence of phytohemagglutinin for ABO bloodgroupsfrom 20 plantspeciesfoundinPuertoPrincesaCity,Palawan. The presence of antibacterial potential was also screened from those which agglutinated one, two, three and four blood types. The agglutination of blood types A, B, AB and O after application with each of the leaf crude extracts was examined macroscopically and microscopically. There were
Keywords:
two trials conducted per treatment per leaf extract. Among the 20 plant species tested, 14 (70%) showed agglutination with at least one blood type while 6 (30%) species did not exhibit agglutination. Two species, the
Palawan Pitcher Plant Nepenthes palawanensis and Cashew Anacardium
occidentale L. agglutinated with all blood types. Four species were found to have the capability of determining a specific blood type: Atsuete (Bixa
orellana L.) and Ilang-ilang (Cananga odorata Lam.) for blood type
O; Balayong (Cassia nodosa Roxb.) for blood type A; and Makabuhay (Tinospora reticulata Miers.) for blood type AB. Cashew, Palawan gabi (Colocasia sp. Schott), Atsuete and Makabuhay leaf extracts were tested
for antibacterial potential against Escherichia coli, Staphylococcus aureus, Bacillus cereus, Bacillus megaterium, Bacillus subtilis and
Pseudomonas aeruginosa. Among the four plants tested, Makabuhay showed antibacterial effects to five bacterial species (E. coli, S. aureus,
B. cereus, B. megaterium and B. subtilis), followed by Atsuete to four bacterial species (S. aureus, B. cereus, B. subtilis and B. megaterium).
Cashew showed only inhibitory effects toward two bacteria (S. aureus and B. megaterium) while Palawan gabi did not inhibit any of the six bacterial species. None of the four extracts inhibited P . a eruginosa.
Hemagglutination Antibacterial Leaf crude extract Blood type Treatment
1College of Fisheries and Aquatic Sciences, Western Philippines University, WPU-Puerto Princesa Campus 2Palawan State University-Brooke’s Point Campus, Brooke’s Point, Palawan
© 2017 Palawan Council for Sustainable Development
1
INTRODUCTION
Agglutination is a process applied such as alkaloids, steroids, tannins, and phenol compounds, flavonoids, steroids, resins, fatty acids and gums which are capable of producing definite physiological action on the body (Joshi and Edington 1990). in immunologic reactions including blood testing and other medical applications (Segen and Stauffer 2004 as cited by Ongpoy 2016). This process involves mixing an antibody with its corresponding antigen such as animal cell, bacteria or red blood cells that forms visible clumps. Blood grouping such as ABO blood groups can be done by observing the clumping of red blood cells in the presence of specific antibody. Agglutination for different blood groups upon application of plant extract is due to the lectin present in plants (George et al. 2011) called phytoagglutinin.
In past decades, higher plants are well recognized for their ability to produce a wide spectrum of natural products with interesting bioactivities (Liu et al. 2007) such as antibacterial potential. Although hundreds of plants have been tested, their antimicrobial properties, many of them are not yet explored (Balandrin et al. 1985).
Palawan, dubbed as the Philippines’
“Last Ecological Frontier”, has various plant species that are either endemic or introduced to theprovinceandarebeingprotectedbytheefforts of the government due to deforestation and over-harvesting. Due to Palawan’s uniqueness, the United Nations Educational Scientific and Cultural Organization (UNESCO), declared it as a “Man and Biosphere Reserve” owing to its vast land area and topography divided by tall mountains ranges (https://pnni.wordpress.com/ palawan-biodiversity-facts-and-figures). With the resources found within the reach, the flora of Palawan must be explored scientifically so they can be used in various applications such as medicine and allied health fields.
Plant lectins are a class of proteins, nonimmune in origin, which show a very specific interaction with carbohydrates (George et al. 2011). Lectins have great significance in the diet because of their involvement in gastrointestinal difficulties and erythrocyte agglutination (Zubcevic et al. 2016). Some lectins consumed by the body act as messengers that has the ability to bind to the sugars of cells in the gut and the blood cells, initiating an inflammatory response. Lectin is abundant on the seed and vegetative parts of a plant including roots, leaves, rhizomes and stems (Chrispeels and Raikhel 1991). It also serves as an important antigenic determinant of blood groups for blood type identification.
This study sought to determine hemagglutination and antibacterial potential of selected plant extracts found in Puerto Princesa, Palawan. Specifically, this study aimed to determine the agglutination of ABO (A, B, AB, and O) blood groups with different plant crude extracts and to determine the antibacterial potential of plant extracts which agglutinated all four blood types, only three blood types, only two blood types and only one blood type.
Hemagglutination activity has been tested in wheat germ agglutinin, peanuts and dry cereal (Brady et al. 1978), tomato lectin (Vasconcelos and Oliveira 2004), navy bean lectin (Pusztai et al. 1979), 20 plant species found in human diet (Zubcevic et al. 2016), in tubers of Eranthis hyemalis (winter aconite) (George et al. 2011) and wild grasses (Ongpoy 2016). There are still many plants unexplored as to their potential in hemagglutination such as those found in Palawan.
MATERIALS AND METHODS Locale of the Study and Collection of Plant Samples
Plantextractshaveenormoustherapeutic potential. They are effective in the treatment of infectious diseases while simultaneously extenuating many of the side effects that are often associated with synthetic antibiotics. The beneficial effects of plant materials typically result from the combinations of secondary products present in the plant. In plants, these compounds are mostly secondary metabolites
This study was conducted at the laboratory of College of Fisheries and Aquatic Sciences (CFAS), Western Philippines University-Puerto Princesa Campus, Bgy. Sta. Monica, Puerto Princesa City.
Twenty plant species were used for this study, collected from different sites in Puerto Princesa City, Palawan. These plants were
© 2017 Palawan Council for Sustainable Development
2
Table 1. List of plants used in this study (Binomial names are based on Merrill ED, 1912, 1923;
Quisumbing 1978; Pancho & Gruezo 2006, 2009; McPherson et al. 2010)
Local Name
Acacia
Scientific Name
Samanea saman (Jacq.) Mer r . Senna alata L.
Acapulco Atsuete
Bixa orellana L.
Bakawan lalake Balayong Balete
Rhizophora apiculata Blume Cassia nodosa Roxb. Ficus indica L.
Calamansi Cashew
Citrus microcarpa Bunge Anacardium occidentale L. Cananga odorata (Lam.) Hook.f. & Thomson Gliricidia sepium (Jacq.) Kunth ex W a lp. Vitex negundo L.
Ilang-ilang Kakawate Lagundi Langka
Artocarpus heterophyllus Lam. Tinospora reticulata Miers. Lantana camara L.
Makabuhay Muyo-muyo Palawaan Pitcher Plant Palawan Gabi Paper Tree Sambong
Nepenthes palawanensis (McPherson et al. 2010) Colocasia sp. Schott Gmelina arborea Roxb. Blumea balsamifera L.
Seresas
Muntingia calabura L.
Tuboy
Ficus sp. L.
chosen randomly based on their availability nearby and based on usage and frequency of encounter by the local people. Some of these plants were endemic to Palawan while others were introduced species from other parts of the Philippines or other countries. The leaves were the plant parts used for this study. The plants were identified in terms of their local and scientific names (Table 1). both sexes who have blood types A, B, AB and O (n=16) (Zubcevic et al., 2016). The blood samples were treated with synthetic anti-sera to confirm blood types. After confirmation, a drop of blood of a specific blood type on a glass slide was mixed with a drop of extract using sterile toothpick and then observed using a microscope (100x magnification). The process was done promptly to avoid blood drying.
Preparation of plant samples and leaf extraction
Antibacterial Assay
Cashew, Atsuete, Palawan gabi and
The leaves were washed with tap water and rinsed with distilled water after collection. The samples were blot dried and air dried for 2 hrs (Figure 1). Extraction was done by pounding through the use of sterile mortar and pestle, followed by squeezing through sterile cheese cloth and filtering with the use of Whatman filter paper.
Makabuhay were chosen to be tested for antibacterial potential as they agglutinated all four blood types, only three blood types, only two blood types and only one blood type, respectively. The leaf crude extracts of these four selected plant species were screened for antibacterial potential using filter paper disc diffusion method against Escherichia
coli, Staphylococcus aureus, Bacillus cereus, Bacillus megaterium, Bacillus subtilis and Pseudomonas aeruginosa which were obtained
from Mindanao State University, Marawi City.
Blood agglutination by different plant leaf
extracts
The blood samples for this study were obtained from healthy willing individuals of
© 2017 Palawan Council for Sustainable Development
3
B
A
Figure 1. a) washing of samples, b) air drying of plant leaves after washing.
The nutrient agar medium was prepared by dissolving 23 grams of nutrient agar in a flask with distilled water to a volume of 1000ml. The melted nutrient was then transferred to 24 test tubes and covered with lids. The test tubes were labeled and were sterilized by autoclaving at 15 psi for 30 minutes. presence or absence of agglutination are presented in Table 2. Among the 20 plant species tested (first and second trial), 14 (70%) agglutinated with at least one blood type while 6 plant species (30%) did not exhibit agglutination (Figure 2). These results of agglutination were higher than the results obtained by Zubcevic et al. (2016) where they found agglutination to only 11 out of 20 plant species. Results of this study also showed that from the 14 plant species where extracts were capable of agglutination, 35.7% agglutinated blood type A, 50% agglutinated blood type B, 85.7% agglutinated blood type AB and 50% agglutinated blood type O (Figure 3). Blood type AB has the highest percentage agglutination which shows its sensitivity to coagulants. This result is similar to the result of Zubcevic et al. (2016) where blood type AB and O showed the highest percentage agglutination when applied with plant extracts found the human diet. There is no study yet that proved differences in sensitivity of blood types A, B, AB and O to different agglutinins and this area is worth doing a study in the future. Seven species showed inconclusive results due to discrepancy in the first and second trials and another trial must be conducted again for verification.
After sterilization, the test tubes with the culture medium were allowed to cool down to about 45 degrees Celsius. The nutrient agar was then inoculated aseptically with a loopful of test bacteria and rotated gently between hands to ensure uniform distribution and then poured aseptically into its respective pre-labelled Petri plates and were allowed to solidify. The prepared filter paper discs (6mm diameter) from no.1 Whatman filter paper that were soaked from the prepared leaf crude extracts were placed aseptically at designated sectors inside the Petri plates. Amoxicillin (500 mg) was used as the positive control and distilled water as the negative control. The Petri plates were then incubated at 35˚ Celsius for 12-24 hours. After incubation period, the plates were examined for zones of inhibition. The zones of inhibition were measured using standardized transparent ruler in millimeter scale.