Cold Hardiness Within the Genus Actinidia
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HORTSCIENCE 30(2):329–332. 1995. intermittent mist (Roche, 1983). Cuttings were transplanted and grown in a plastic green- house until May 1991. In winter, all cuttings Cold Hardiness within the Genus were replanted into 5-liter pots containing 3 leaf-mold : 1 peat : 1 sand (by volume) and cut Actinidia back to 20 cm above ground level. In May 1991, vines were transferred to an outdoor site Joëlle Chat for natural cold acclimation until they were Institut National de la Recherche Agronomique, Station de Recherches tested. The pots were watered daily and fertil- ized from May to July 1991 with 15N–15P– Fruitières, B.P. 81, 33883 Villenave D’Ornon Cedex, France 15K for a total application rate of 30 g/pot. Additional index words. Actinidia arguta, Actinidia deliciosa, Actinidia kolomikta, Actinidia Freezing tests. Two tests were carried out polygama, artificial freezing test, freezing tolerance, kiwifruit with a 3-day interval on 19 and 22 Feb. 1992. The first test involved A. deliciosa, the second Abstract. Two-year-old Actinidia vines, grown on their own roots, were subjected to involved the other species. All vines were artificial freezing tests in midwinter to determine their relative hardiness. Plant survival, subjected to the same temperature regime. growth recovery, and stem necrosis were used for estimating freezing injury. Actinidia One clone of a female A. arguta and ‘Hayward’ deliciosa (A. Chev.) C.F. Liang & A.R. Ferguson var. deliciosa vines, which included were used as reference genotypes in each test. ‘Abbott’, ‘Bruno’, ‘Greensill’, ‘Hayward’, and ‘Jones’ kiwifruit, were all severely dam- In addition, two vines per genotype, not sub- aged by exposure to a temperature of –18C for 4 hours. Actinidia arguta (Sieb. et Zucc.) jected to any artificial freezing, were used as Planch. ex Miq., A. kolomikta (Maxim. et Rupr.) Maxim., and A. polygama (Sieb. et Zucc.) controls. A 300 × 345 × 200-cm chamber, Maxim. appeared to be more tolerant to winter cold than A. deliciosa, indicating that capable of precisely reproducing advective potential germplasm exists for improvement of cold hardiness through interspecific freezing conditions, was used. Air tempera- hybridization. ture could be controlled from 10 to –25C. Pots were placed within the chamber in a 280 × 210 Freezing temperatures may cause serious buds in the lower leaf axils (Brundell, 1975). × 25-cm Styrofoam box and kept in darkness yield losses in kiwifruit, particularly in Eu- Young shoots can be damaged by exposure to throughout the experiment. Two 8-cm-thick rope. Previous observations have indicated –0.5C (Pyke et al., 1986) and flower buds can glass wool insulation layers, surrounded by that kiwifruit vines may incur serious freezing be killed at –1.5C (Hewett and Young, 1981). polythene, covered the pots, thus reducing the injury in spring on young shoots and flower In autumn, kiwifruit maturity is reached later incidence and duration of root system expo- buds, in autumn on fruits and leaves, and in than for other deciduous fruit trees. Thus, the sure to freezing injury. All the vines were first winter on trunk, canes, and dormant buds, as fruit may be damaged by early unexpected placed in the controlled-environment cham- observed in many European kiwifruit orchards freezing before harvest (Ferguson et al., 1990). ber at a constant 6C before the freezing test. in Jan. 1985 (Blanchet, 1985). Limited information is available on the The air temperature program included five The threshold level of freezing tolerance of cold hardiness of specific cultivars or species sequential 4-h steps at –10, –2, –18, –2, and kiwifruit vines in winter under field conditions of Actinidia, although some botanical species –10C. Alternating freezing temperatures were varies from –10C to –18C (Dozier et al., 1992; are reported to be more cold hardy than A. selected for the temperature regime since this Kamota et al., 1989; Monet and Bastard, 1985). deliciosa ‘Hayward’, the most commonly simulated best the natural freezing conditions Damage to buds occurs on dormant vines at planted cultivar. Five species of Actinidia are that occurred in France during Jan. 1985. Freez- temperatures below –10C (Hewett and Young, reported by Avery (1991) to be hardier than A. ing and thawing were programmed at a rate of 1981), consisting of an increase in the propor- deliciosa and are as follows: A. arguta, A. either 1 or 2C/h. A temperature sensor, located tion of dead buds and a subsequent decrease in kolomikta, A. melanandra, A. polygama, and in the air-conditioning machinery area of the the proportion of fruit-bearing shoots (Testolin A. purpurea. Chat (1994) has previously con- chamber, measured the temperature of the and Messina, 1987). Exposure to temperatures firmed the superior cold hardiness of three of return air flow. The air temperature measured below –18C can cause more serious damage, these species. The objective of the present in the chamber differed from that of the sensor as evidenced by bark splitting of the trunk research was to determine the degree of freez- by up to +2C, when temporal variation was (Monet and Bastard, 1985; Weet, 1979). The ing tolerance of container-grown Actinidia restricted to 1C. After the temperature had resumption of growth in the orchard in early vines belonging to A. deliciosa, the cultivated returned to 6C, vines were removed from the spring predisposes the kiwifruit vine to the species, as well as wild species, to identify chamber and transferred to the greenhouse. risk of cold damage. Dormant buds, deeply suitable parental genotypes for a breeding Evaluating freezing injury. Vines were embedded in corky tissue during winter, re- program. Exposure to natural freezing condi- forced in the greenhouse for 6 weeks at a mean quire little chilling to overcome rest (Brundell, tions is the oldest and ultimate test of plant 1976) and often burst in March in France cold hardiness, with the major drawback that (Blanchet, 1985). On a mature vine, young experimental results are not reproducible and Table 1. Actinidia species and clones tested for shoots, arising from dormant buds, bear flower therefore difficult to interpret (Li, 1984). The freezing tolerance in 1992. most reliable method for assessing freezing Clone tolerance involves subjecting entire plants or Species Sexz Cultivar code plant parts to artificial cold treatment and A. deliciosa F Abbott W6 evaluating the subsequent recovery and tissue F Bruno W7 damage incurred (Quamme, 1978). F Greensill W8 Received for publication 5 Aug. 1994. Accepted for F Jones W9 F Hayward K5 publication 3 Nov. 1994. I thank A. Escobar for Materials and Methods helpful discussions; F. Dosba, R. Monet, and A. M --- K7 Moing for critical reading of the manuscript; M. M --- K8 Plant material. Seven A. deliciosa clones A. arguta F --- W20 Guye for the English language corrections; and A. and six clones belonging to other Actinidia Bonnet for technical assistance. This research was F --- W16 supported in part by the European Community (Con- species were used in this study (Table 1). K7 M --- W4 tract CT 91010). The cost of publishing this paper and K8 are male clones selected at the Institut A. kolomikta F --- W12 was defrayed in part by the payment of page charges. National de la Recherche Agronomique Fruit M --- W10 Under postal regulations, this paper therefore must Research Station orchard of Bordeaux, France. A. polygama F --- W18 be hereby marked advertisement solely to indicate Semi-hardwood cuttings treated in July 1990 zF and M indicate a female and a male plant, respec- this fact. with indole-3-butyric acid were rooted under tively. HORTSCIENCE, VOL. 30(2), APRIL 1995 329 BREEDING, CULTIVARS, ROOTSTOCKS, & GERMPLASM RESOURCES of 22C. The air ranged from a 35C daytime freezing injury on the terminal portion of the A. arguta (W16) and A. deliciosa ‘Hayward’ maximum to a 15C nighttime minimum. The shoot, and cessation of growth. Terminal stem (K5) showed similar levels of cold hardiness date at which growth resumed was noted for necrosis, due to natural freezing in Fall 1991 in the 19 and 22 Feb. tests. Under our experi- each vine. Each vine was assessed for survival and Winter 1992, ranged from 5 to 32 cm for mental conditions, the 3-day interval between and freezing injury 6 weeks after the freezing A. deliciosa, and from 2 to 34 cm for the other the two freezing tests did not appear to modify test. Vines that showed no regrowth on this species (see controls in Tables 3 and 4). This the freezing tolerance of the Actinidia vines. date were rated as dead. Freezing injury was experiment does not permit determining For necrosis length, an ANOVA for data col- estimated by the damage exhibited by the whether these differences are due to variation lected following the two freezing tests indi- main stem. Undamaged stems remained green in temperature among the pots within the out- cated no significant effect due to the blocks but and firm, while damaged stems first darkened door site or to variation in freezing susceptibil- a highly significant effect due to genotypes. and ultimately dried out. Necrosis length (cen- ity among genotypes. Within A. deliciosa, necrosis length ap- timeters) was expressed as the length of the Damage due to artificial freezing. All the peared to vary greatly depending on the geno- main stem showing necrotic lesions. Necrosis vines subjected to the artificial freezing test type, although it increased in direct ratio to percentage was based on necrosis length rela- exhibited more freezing injury than the con- plant height (Table 3). The A. deliciosa clone tive to plant height (Lu and Rieger, 1990). trols when considering each genotype sepa- K8 exhibited the smallest amount of freezing Statistical analysis. The design was a ran- rately (Tables 3 and 4).