Investigating Root-Knot and Soybean Cyst Nematode Parasitic Interactions Through Transcriptomic Analyses of the Host and Parasite
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Engineered Soybean Cyst Nematode Resistance
Chapter 6 Engineered Soybean Cyst Nematode Resistance Vincent P. Klink, Prachi D. Matsye, Katheryn S. Lawrence and Gary W. Lawrence Additional information is available at the end of the chapter http://dx.doi.org/10.5772/54514 1. Introduction A variety of plant parasitic nematodes (PPNs), including the soybean cyst nematode (SCN), elicit the initiation, development and maintenance of a specialized nurse cell from which they derive their nutriment (Figure 1). Remarkably, during parasitism by the PPN, the nurse cell survives the apparently significant resource drain on the root cell that would be expect‐ ed to detrimentally impact normal physiological processes of the cell. This outcome indi‐ cates that the nematode has developed a well tuned apparatus to ensure that the root cell does not collapse and die during parasitism. In contrast, in the soybean-SCN pathosystem, the nurse cell and sometimes the surrounding cells are the sites of the defense response to the parasite (Ross, 1958; Endo, 1965). Therefore, plants have in place a mechanism to over‐ come the influence of the activities of the nematode. Identifying the factor(s) is of utmost im‐ portance in developing resistance to PPNs. 1.2. History Documented accounts reveal that soybean has been in cultivation for thousands of years (Hymowitz et al. 1970), beginning in Asia perhaps as early as 3,500 B.C. (Liu et al. 1997). While the natural range of soybean is East Asia, after thousands of years of cultivation a true understanding of its native range is complicated at best. However, the extensive range of wild soybean and obvious differences in its growth habit indicates that while environmental cues may be responsible for changes in soybean and plant growth habit in general (Garner Allard 1930; Chapin III et al. -
Allele-Specific Expression of Ribosomal Protein Genes in Interspecific Hybrid Catfish
Allele-specific Expression of Ribosomal Protein Genes in Interspecific Hybrid Catfish by Ailu Chen A dissertation submitted to the Graduate Faculty of Auburn University in partial fulfillment of the requirements for the Degree of Doctor of Philosophy Auburn, Alabama August 1, 2015 Keywords: catfish, interspecific hybrids, allele-specific expression, ribosomal protein Copyright 2015 by Ailu Chen Approved by Zhanjiang Liu, Chair, Professor, School of Fisheries, Aquaculture and Aquatic Sciences Nannan Liu, Professor, Entomology and Plant Pathology Eric Peatman, Associate Professor, School of Fisheries, Aquaculture and Aquatic Sciences Aaron M. Rashotte, Associate Professor, Biological Sciences Abstract Interspecific hybridization results in a vast reservoir of allelic variations, which may potentially contribute to phenotypical enhancement in the hybrids. Whether the allelic variations are related to the downstream phenotypic differences of interspecific hybrid is still an open question. The recently developed genome-wide allele-specific approaches that harness high- throughput sequencing technology allow direct quantification of allelic variations and gene expression patterns. In this work, I investigated allele-specific expression (ASE) pattern using RNA-Seq datasets generated from interspecific catfish hybrids. The objective of the study is to determine the ASE genes and pathways in which they are involved. Specifically, my study investigated ASE-SNPs, ASE-genes, parent-of-origins of ASE allele and how ASE would possibly contribute to heterosis. My data showed that ASE was operating in the interspecific catfish system. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5,420 (8.2%) and 13,390 (7.5%) SNPs were identified as significant ASE-SNPs, respectively. -
Heterodera Glycines Ichinohe) in Dry Bean (Phaseolus Vulgaris L.
Evaluating Seed Treatments for the Management of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Dry Bean (Phaseolus vulgaris L.) by Trust T. Katsande A Thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Master of Science in Plant Agriculture Guelph, Ontario, Canada © Trust T. Katsande, September, 2019 ABSTRACT Evaluating Seed Treatments for the Management of Soybean Cyst Nematode (Heterodera glycines Ichinohe) in Dry Bean (Phaseolus vulgaris L.) Trust T. Katsande Advisor: University of Guelph, 2019 Chris L. Gillard Soybean cyst nematode (Heterodera glycines; SCN) infestation is a major cause of yield loss in soybean (Glycine max), and dry bean (Phaseolus vulgaris) is an alternative host. In soybean, genetic resistance and seed treatments are mainly used for SCN management however these options are not available in dry bean. Seven seed treatments were assessed for effects on SCN populations in black (cv. Zorro) and kidney (cv. Dynasty; Red Hawk) bean. Two field studies were conducted in 2018 on naturally infested soils near Highgate and Rodney, Ontario. In addition, two different controlled environment studies were completed. There was little treatment response in field studies. In the first controlled environment study, Bacillus amyloliquefaciens and Bacillus firmus reduced cysts in black and kidney bean while fluopyram reduced cysts in Red Hawk only. In second study, fluopyram reduced cysts by 50% and 88% in Dynasty and Red Hawk, respectively while other treatments were inconsistent. iii DEDICATION This dissertation is dedicated to my caring and loving parents Michael and Sarudzai Katsande, my brothers Austin and Omega for their endless support. -
Investigation of the Development of Root Lesion Nematodes, Pratylenchus Spp
Türk. entomol. derg., 2021, 45 (1): 23-31 ISSN 1010-6960 DOI: http://dx.doi.org/10.16970/entoted.753614 E-ISSN 2536-491X Original article (Orijinal araştırma) Investigation of the development of root lesion nematodes, Pratylenchus spp. (Tylenchida: Pratylenchidae) in three chickpea cultivars Kök lezyon nematodlarının, Pratylenchus spp. (Tylenchida: Pratylenchidae) üç nohut çeşidinde gelişmesinin incelenmesi İrem AYAZ1 Ece B. KASAPOĞLU ULUDAMAR1* Tohid BEHMAND1 İbrahim Halil ELEKCİOĞLU1 Abstract In this study, penetration, population changes and reproduction rates of root lesion nematodes, Pratylenchus neglectus (Rensch, 1924), Pratylenchus penetrans (Cobb, 1917) and Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae), at 3, 7, 14, 21, 28, 35, 42, 49 and 56 d after inoculation in chickpea Bari 2, Bari 3 (Cicer reticulatum Ladiz) and Cermi [Cicer echinospermum P.H.Davis (Fabales: Fabaceae)] were assessed in a controlled environment room in 2018-2019. No juveniles were observed in the roots in the first 3 d after inoculation. Although, population density of P. thornei reached the highest in Cermi (21 d), Bari 3 (42 d) and the lowest observed on Bari 2. Pratylenchus neglectus reached the highest population density in Bari 3 and Cermi on day 28. The population density of P. neglectus was the lowest in Bari 2. Also, population density of P. penetrans reached the highest in Bari 3 cultivar within 49 d, similar to P. thornei, whereas Bari 2 and Cermi had low population densities during the entire experimental period. Keywords: -
1 Reproductive Efficiency of Entomopathogenic Nematodes As Scavengers. Are They Able to 1 Fight for Insect's Cadavers?
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Sapientia 1 Reproductive efficiency of entomopathogenic nematodes as scavengers. Are they able to 2 fight for insect’s cadavers? 3 4 Rubén Blanco-Péreza,b, Francisco Bueno-Palleroa,b, Luis Netob, Raquel Campos-Herreraa,b,* 5 6 a MeditBio, Centre for Mediterranean Bioresources and Food, Universidade do Algarve, Campus 7 de Gambelas, 8005, Faro (Portugal) 8 b Universidade do Algarve, Campus de Gambelas, 8005, Faro (Portugal) 9 10 *Corresponding author 11 Email: [email protected] 12 1 13 Abstract 14 15 Entomopathogenic nematodes (EPNs) and their bacterial partners are well-studied insect 16 pathogens, and their persistence in soils is one of the key parameters for successful use as 17 biological control agents in agroecosystems. Free-living bacteriophagous nematodes (FLBNs) in 18 the genus Oscheius, often found in soils, can interfere in EPN reproduction when exposed to live 19 insect larvae. Both groups of nematodes can act as facultative scavengers as a survival strategy. 20 Our hypothesis was that EPNs will reproduce in insect cadavers under FLBN presence, but their 21 reproductive capacity will be severely limited when competing with other scavengers for the same 22 niche. We explored the outcome of EPN - Oscheius interaction by using freeze-killed larvae of 23 Galleria mellonella. The differential reproduction ability of two EPN species (Steinernema 24 kraussei and Heterorhabditis megidis), single applied or combined with two FLBNs (Oscheius 25 onirici or Oscheius tipulae), was evaluated under two different infective juvenile (IJ) pressure: 26 low (3 IJs/host) and high (20 IJs/host). -
Summary Paratrichodorus Minor Is a Highly Polyphagous Plant Pest, Generally Found in Tropical Or Subtropical Soils
CSL Pest Risk Analysis for Paratrichodorus minor copyright CSL, 2008 CSL PEST RISK ANALYSIS FOR Paratrichodorus minor Abstract/ Summary Paratrichodorus minor is a highly polyphagous plant pest, generally found in tropical or subtropical soils. It has entered the UK in growing media associated with palm trees and is most likely to establish on ornamental plants grown under protection. There is a moderate likelihood of the pest establishing outdoors in the UK through the planting of imported plants in gardens or amenity areas. However there is a low likelihood of the nematode spreading from such areas to commercial food crops, to which it presents a small risk of economic impact. P. minor is known to vector the Tobacco rattle virus (TRV), which affects potatoes, possibly strains that are not already present in the UK, but the risk of the nematode entering in association with seed potatoes is low. Overall the risk of P. minor to the UK is rated as low. STAGE 1: PRA INITIATION 1. What is the name of the pest? Paratrichodorus minor (Colbran, 1956) Siddiqi, 1974 Nematode: Trichodoridae Synonyms: Paratrichodorus christiei (Allen, 1957) Siddiqi, 1974 Paratrichodorus (Nanidorus) christiei (Allen, 1957) Siddiqi, 1974 Paratrichodorus (Nanidorus) minor (Colbran, 1956) Siddiqi, 1974 Trichodorus minor Colbran, 1956 Trichodorus christiei Allen, 1957 Nanidorus minor (Colbran, 1956) Siddiqi, 1974 Nanidorus christiei (Allen, 1957) Siddiqi, 1974 Trichodorus obesus Razjivin & Penton, 1975 Paratrichodorus obesus (Razjivin & Penton, 1975) Rodriguez-M. & Bell, 1978. Paratrichodorus (Nanidorus) obesus (Razjivin & Penton, 1975) Rodriguez-M. & Bell, 1978. Common names: English: a stubby-root nematode. References: Decraemer, 1995 In Europe there has been some confusion between P. -
Management Strategies for Control of Soybean Cyst Nematode and Their Effect on Nematode Community
Management Strategies for Control of Soybean Cyst Nematode and Their Effect on Nematode Community A Thesis SUBMITTED TO THE FACULTY OF UNIVERSITY OF MINNESOTA BY Zane Grabau IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF SCIENCE Dr. Senyu Chen June 2013 © Zane Grabau 2013 Acknowledgements I would like to acknowledge my committee members John Lamb, Robert Blanchette, and advisor Senyu Chen for their helpful feedback and input on my research and thesis. Additionally, I would like to thank my advisor Senyu Chen for giving me the opportunity to conduct research on nematodes and, in many ways, for making the research possible. Additionally, technicians Cathy Johnson and Wayne Gottschalk at the Southern Research and Outreach Center (SROC) at Waseca deserve much credit for the hours of technical work they devoted to these experiments without which they would not be possible. I thank Yong Bao for his patient in initially helping to train me to identify free-living nematodes and his assistance during the first year of the field project. Similarly, I thank Eyob Kidane, who, along with Senyu Chen, trained me in the methods for identification of fungal parasites of nematodes. Jeff Vetsch from SROC deserves credit for helping set up the field project and advising on all things dealing with fertilizers and soil nutrients. I want to acknowledge a number of people for helping acquire the amendments for the greenhouse study: Russ Gesch of ARS in Morris, MN; SROC swine unit; and Don Wyse of the University of Minnesota. Thanks to the University of Minnesota Plant Disease Clinic for contributing information for the literature review. -
First Report of Stubby Root Nematode, Paratrichodorus Teres (Nematoda: Trichodoridae) from Iran
Australasian Plant Dis. Notes (2014) 9:131 DOI 10.1007/s13314-014-0131-4 First report of stubby root nematode, Paratrichodorus teres (Nematoda: Trichodoridae) from Iran R. Heydari & Z. Tanha Maafi & F. Omati & W. Decraemer Received: 8 October 2013 /Accepted: 20 March 2014 /Published online: 4 April 2014 # Australasian Plant Pathology Society Inc. 2014 Abstract During a survey of plant-parasitic nematodes in fruit Trichodorus, Nanidorus and Paratrichodorus are natural tree nurseries in Iran, a species of the genus Paratrichodorus vectors of the plant Tobraviruses occurring worldwide from the family Trichodoridae was found in the rhizosphere of (Taylor and Brown 1997; Decraemer and Geraert 2006). apricot seedlings in Shahrood, central Iran, then subsequently in Eight species of the Trichodoridae family have so far been Karaj orchards. Morphological and morphometric characters of reported from Iran: Trichodorus orientalis (De Waele and the specimens were in agreement with P. teres.TheD2/D3 Hashim 1983), T. persicus (De Waele and Sturhan 1987), expansion fragment of the large subunit (LSU) of rRNA gene T. gilanensis, T. primitivus, Paratrichodorus porosus, P. of the nematode was also sequenced. P. teres is considered an tunisiensis (Maafi and Decraemer 2002), T. arasbaranensis economically important species in agricultural crop, worldwide. (Zahedi et al. 2009)andP. mi no r (now Nanidorus minor) This is the first report of the occurrence of P. teres in Iran. (Pourjam et al. 2011). Several species were detected in a survey conducted on Keywords Apricot . Fruit tree nursery . Iran . plant-parasitic nematodes in fruit tree nurseries. Among Paratrichodorus teres them, a nematode population belonging to Trichodoridae was observed in the rhizosphere of apricot seedlings in Shahroood, Semnan province, central Iran, that was sub- Trichodorid nematodes are root ectoparasites, usually sequently identified as P. -
A Synopsis of the Genera and Species in the Tylenchorhynchinae (Tylenchoidea, Nematoda)1
OF WASHINGTON, VOLUME 40, NUMBER 1, JANUARY 1973 123 Speer, C. A., and D. M. Hammond. 1970. tured bovine cells. J. Protozool. 18 (Suppl.): Development of Eimeria larimerensis from the 11. Uinta ground squirrel in cell cultures. Ztschr. Vetterling, J. M., P. A. Madden, and N. S. Parasitenk. 35: 105-118. Dittemore. 1971. Scanning electron mi- , L. R. Davis, and D. M. Hammond. croscopy of poultry coccidia after in vitro 1971. Cinemicrographic observations on the excystation and penetration of cultured cells. development of Eimeria larimerensis in cul- Ztschr. Parasitenk. 37: 136-147. A Synopsis of the Genera and Species in the Tylenchorhynchinae (Tylenchoidea, Nematoda)1 A. C. TARJAN2 ABSTRACT: The genera Uliginotylenchus Siddiqi, 1971, Quinisulcius Siddiqi, 1971, Merlinius Siddiqi, 1970, Ttjlenchorhynchus Cobb, 1913, Tetylenchus Filipjev, 1936, Nagelus Thome and Malek, 1968, and Geocenamus Thorne and Malek, 1968 are discussed. Keys and diagnostic data are presented. The following new combinations are made: Tetylenchus aduncus (de Guiran, 1967), Merlinius al- boranensis (Tobar-Jimenez, 1970), Geocenamus arcticus (Mulvey, 1969), Merlinius brachycephalus (Litvinova, 1946), Merlinius gaudialis (Izatullaeva, 1967), Geocenamus longus (Wu, 1969), Merlinius parobscurus ( Mulvey, 1969), Merlinius polonicus (Szczygiel, 1970), Merlinius sobolevi (Mukhina, 1970), and Merlinius tatrensis (Sabova, 1967). Tylenchorhynchus galeatus Litvinova, 1946 is with- drawn from the genus Merlinius. The following synonymies are made: Merlinius berberidis (Sethi and Swarup, 1968) is synonymized to M. hexagrammus (Sturhan, 1966); Ttjlenchorhynchus chonai Sethi and Swarup, 1968 is synonymized to T. triglyphus Seinhorst, 1963; Quinisulcius nilgiriensis (Seshadri et al., 1967) is synonymized to Q. acti (Hopper, 1959); and Tylenchorhynchus tener Erzhanova, 1964 is regarded a synonym of T. -
Towards Universal Forward Genetics: Using a Draft Genome Sequence of the Nematode Oscheius Tipulae to Identify Mutations Affecting Vulva Development
Genetics: Early Online, published on June 19, 2017 as 10.1534/genetics.117.203521 Towards universal forward genetics: using a draft genome sequence of the nematode Oscheius tipulae to identify mutations affecting vulva development 5 Fabrice Besnard*1,2,3, Georgios Koutsovoulos†1,4, Sana Dieudonné*, Mark Blaxter†,5 and Marie-Anne Félix*2,5 10 * Ecole Normale Supérieure, PSL Research University, CNRS, Inserm, Institut de Biologie de l'Ecole Normale Supérieure, 75005 Paris, France † Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, UK 1 Co-first authors. 2 Co-corresponding authors: [email protected], [email protected] 3 Current Address: Laboratoire de Reproduction de développement des plantes, Lyon, France; 15 4 Current Address: INRA, Université Côte d’Azur, CNRS, ISA, France 5 Co-last authors Besnard F., Koutsovoulos G. et al. Oscheius Mapping-by-sequencing 1/48 Copyright 2017. Running title: Oscheius Mapping-by-sequencing Key words : Oscheius tipulae, genome assembly, mapping-by sequencing, vulva development, mig-13 Co-corresponding authors: Fabrice Besnard Address: Laboratoire Reproduction et Développement des Plantes (RDP) 20 Ecole Normale Supérieure de Lyon (ENS-Lyon) 46, allée d'Italie, 69364 LYON Cedex 07. Tel: +33-4-72-72-86-05 mail: [email protected] Marie-Anne Félix Address: Institute of Biology of the Ecole Normale Supérieure (IBENS) 25 46 rue d'Ulm, 75230 Paris cedex 05, France Tel: +33-1-44-32-39-44 mail: [email protected] Besnard F., Koutsovoulos G. et al. Oscheius Mapping-by-sequencing 2/48 Abstract Mapping-by-sequencing has become a standard method to map and identify phenotype-causing mutations in model species. -
JOURNAL of NEMATOLOGY Morphological And
JOURNAL OF NEMATOLOGY Article | DOI: 10.21307/jofnem-2020-098 e2020-98 | Vol. 52 Morphological and molecular characterization of Heterodera dunensis n. sp. (Nematoda: Heteroderidae) from Gran Canaria, Canary Islands Phougeishangbam Rolish Singh1,2,*, Gerrit Karssen1, 2, Marjolein Couvreur1 and Wim Bert1 Abstract 1Nematology Research Unit, Heterodera dunensis n. sp. from the coastal dunes of Gran Canaria, Department of Biology, Ghent Canary Islands, is described. This new species belongs to the University, K.L. Ledeganckstraat Schachtii group of Heterodera with ambifenestrate fenestration, 35, 9000, Ghent, Belgium. presence of prominent bullae, and a strong underbridge of cysts. It is characterized by vermiform second-stage juveniles having a slightly 2National Plant Protection offset, dome-shaped labial region with three annuli, four lateral lines, Organization, Wageningen a relatively long stylet (27-31 µm), short tail (35-45 µm), and 46 to 51% Nematode Collection, P.O. Box of tail as hyaline portion. Males were not found in the type population. 9102, 6700, HC, Wageningen, Phylogenetic trees inferred from D2-D3 of 28S, partial ITS, and 18S The Netherlands. of ribosomal DNA and COI of mitochondrial DNA sequences indicate *E-mail: PhougeishangbamRolish. a position in the ‘Schachtii clade’. [email protected] This paper was edited by Keywords Zafar Ahmad Handoo. 18S, 28S, Canary Islands, COI, Cyst nematode, ITS, Gran Canaria, Heterodera dunensis, Plant-parasitic nematodes, Schachtii, Received for publication Systematics, Taxonomy. September -
Rapid Pest Risk Analysis (PRA) For: Stage 1: Initiation
Rapid Pest Risk Analysis (PRA) for: Globodera tabacum s.I. November 2014 Stage 1: Initiation 1. What is the name of the pest? Preferred scientific name: Globodera tabacum s.l. (Lownsbery & Lownsbery, 1954) Skarbilovich, 1959 Other scientific names: Globodera tabacum solanacearum (Miller & Gray, 1972) Behrens, 1975 syn. Heterodera solanacearum Miller & Gray, 1972 Heterodera tabacum solanacearum Miller & Gray, 1972 (Stone, 1983) Globodera Solanacearum (Miller & Gray, 1972) Behrens, 1975 Globodera Solanacearum (Miller & Gray, 1972) Mulvey & Stone, 1976 Globodera tabacum tabacum (Lownsbery & Lownsbery, 1954) Skarbilovich, 1959 syn. Heterodera tabacum Lownsbery & Lownsbery, 1954 Globodera tabacum (Lownsbery & Lownsbery, 1954) Behrens, 1975 Globodera tabacum (Lownsbery & Lownsbery, 1954) Mulvey & Stone, 1976 Globodera tabacum virginiae (Miller & Gray, 1968) Stone, 1983 syn. Heterodera virginiae Miller & Gray, 1968 Heterodera tabacum virginiae Miller & Gray, 1968 (Stone, 1983) Globodera virginiae (Miller & Gray, 1968) Stone, 1983 Globodera virginiae (Miller & Gray, 1968) Behrens, 1975 Globodera virginiae (Miller & Gray, 1968) Mulvey & Stone, 1976 Preferred common name: tobacco cyst nematode 1 This PRA has been undertaken on G. tabacum s.l. because of the difficulties in separating the subspecies. Further detail is given below. After the description of H. tabacum, two other similar cyst nematodes, colloquially referred to as horsenettle cyst nematode and Osbourne's cyst nematode, were later designated by Miller et al. (1962) from Virginia, USA. These cyst nematodes were fully described and named as H. virginiae and H. solanacearum by Miller & Gray (1972), respectively. The type host for these species was Solanum carolinense L.; other hosts included different species of Nicotiana, Physalis and Solanum, as well as Atropa belladonna L., Hycoscyamus niger L., but not S.