i Protein & Letters, 2016, Vol. 23, No. 12 Graphical Abstracts

Graphical Abstracts

Protein & Peptide Letters; Vol. 23; 2016, 1045 Hemopressin as Modulators of the and their Potential Applications as Therapeutic Tools Giorgia Macedonio, Azzurra Stefanucci, Cristina Maccallini, Sako Mirzaie, Ettore Novellino and Adriano Mollica* Dipartimento di Farmacia, Universita` di Chieti-Pescara ‘‘G. d’Annunzio’’, Chieti, Italy The Endocannabinoid system (ECS) involves the hydrolytic enzymes FAAH and MAGL, which are possible therapeutic α1- chain Arachidonic acid targets for the development of new drugs as indirect Endocannabinoid System . Recently it has been discovered a new family of

endocannabinoid modulators; the lead of this family is the Peptidic Endocannabinoids CB1 Lipidic Endocannabinoids nonapeptide Hemopressin which derives from the α-chain of (e.g. RVD-Hemopressin) (e.g. ) hemoglobin and acts as negative allosteric modulator of CB1.

Protein & Peptide Letters; Vol. 23; 2016, 1052 Fish Hydrolysates: A Regulatory Perspective of Bioactive Peptides Bert Gevaert, Lieselotte Veryser, Frederick Verbeke, Evelien Wynendaele and Bart De Spiegeleer* Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, B-9000 Ghent, Belgium Fish hydrolysates are produced from various types of fish. After isolating the fish material, the mixture is digested under specific conditions after which the obtained peptide mixture is purified. Bioactivities like blood-sugar lowering effects, antihypertensive effects, hunger control and stress relief have been reported.

Protein & Peptide Letters; Vol. 23; 2016, 1061 Proline-rich Antimicrobial Peptides Optimized for Binding to Escherichia coli Chaperone DnaK Daniel Knappe*, Tina Goldbach, Marcus P.D. Hatfield, Nicholas Y. Palermo, Stefanie Weinert, Norbert Sträter, Ralf Hoffmann and Sándor Lovas* Institute für Bioanalytische Chemie, Biotechnologisch-Biomedizinisches Zentrum, Universität Leipzig, Deutscher Platz 5, 04103 Leipzig, Germany

Molecular modeling Fluorescence polarization

-162.5 KJ/mol MD and rational design 120

generated improved peptide [mP] Onc72 Trp-Leu-Trp-Phe ligands for the substrate 80 Asn-Trp-His-Lys binding domain of DnaK.

Enhanced dissociation 40 constants for the corresponding synthetic 0 peptides were proven by

Fluorescence polarization -5 -4 -3 -2 -1 0 1 2 3 fluorescence polarization. 10 10 10 10 10 10 10 10 10 Asn-Trp-His-Lys DnaK protein concentration [mmol/L]

Graphical Abstracts Protein & Peptide Letters, 2016, Vol. 23, No. 12 ii

Protein & Peptide Letters; Vol. 23; 2016, 1072 α-Lactalbumin: Of Camels and Cows Jennifer M. Redington, Leonid Breydo, Hussein A. Almehdar, Elrashdy M. Redwan and Vladimir N. Uversky* Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, 12901 Bruce B. Downs blvd., MDC07, Tampa, Florida 33612, USA Comparative structural analysis of bovine and camel α- lactalbumins revealed that camel protein is more stable towards thermal and pH-mediated denaturation but less stable towards guanidine hydrochloride-mediated unfolding, aggregates faster and is predicted to be more disordered than the bovine protein.

Protein & Peptide Letters; Vol. 23; 2016, 1081 OPMSP: A Computational Method Integrating Protein Interaction and Sequence Information for the Identification of Novel Putative Oncogenes

1000 randomly produced sets Lei Chen*, Baoman Wang, ShaoPeng Wang, Jing Yang, Oncogenes Jerry Hu, ZhiQun Xie, Yuwei Wang, Tao Huang* and … Yu-Dong Cai*

PPI College of Information Engineering, Shanghai Maritime information Randomization test University, People’s Republic of China

A computational method, namely OPMSP, was proposed in this study to identify novel oncogenes. The method yielded thirty-seven novel genes that were extensively analyzed. …

Candidate genes filtered Candidate genes Shortest paths connecting by a randomization test any two oncogenes Linkages with known oncogenes using protein interaction and sequence information

Putative oncogenes

Protein & Peptide Letters; Vol. 23; 2016, 1103 Discovery and Biochemical Characterization of the UDP-Xylose Biosynthesis Pathway in Sphaerobacter thermophilus Bin Gu, Pedro Laborda, Shuang Wei, Xu-Chu Duan, Hui-Bo Song, Li Liu* and Josef Voglmeir* Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology, Nanjing Agricultural University, Nanjing, People’s Republic of China Revealing the UDP-xylose biosynthesis pathway of the biotechnologically relevant microorganism Sphaerobacter thermophiles.

iii Protein & Peptide Letters, 2016, Vol. 23, No. 12 Graphical Abstracts

Protein & Peptide Letters; Vol. 23; 2016, 1111 Acid-Induced Unfolding of Champedak Galactose-Binding Lectin

Nurul Iman A. Kameel, Adawiyah S. Shuib and Saad Tayyab* 4500 4000 Biomolecular Research Group, Biochemistry Programme, 3500 Institute of Biological Sciences, Faculty of Science, University 3000 Fluorescence Intensity at 330 nm (Ex. 280 nm) nm) 280 330 nm (Ex. at 2500 0 2 4 6 8 of Malaya, 50603, Kuala Lumpur, Malaysia pH

Acid-denaturation of CGB lectin, as studied by ANS fluorescence and intrinsic fluorescence (inset) measurements

Protein & Peptide Letters; Vol. 23; 2016, 1118 Biophysical Characterization of Alanine Aminotransferase from Trypanosoma cruzi Stephanie B. de Morais, Tatiana de Arruda Campos Brasil de Souza*, Ana V.P. Weiler and Mario T. Murakami Instituto Carlos Chagas, FIOCRUZ-PR, Rua Algacyr Munhoz Mader, 3775, 81150010, Curitiba, PR, Brazil Alanine aminotransferase from Trypanosoma cruzi (TcALAT) was expressed as a recombinant in Escherichia coli and purified by different chromatographic methods. Its secondary structure content, stability and in solution behavior were investigated in this work.

Protein & Peptide Letters; Vol. 23; 2016, 1123 Manipulation of Intracellular pH in Cancer Cells by NHE1 Inhibitors Francesca Aredia and Anna I. Scovassi* TUMOR Istituto di Genetica Molecolare CNR, Via Abbiategrasso 207, PROGRESSION 27100 Pavia, Italy NHE NHE + + NHEs and cancer. Na /H exchangers (NHEs) affect cancer cell behavior OVERACTIVATION INHIBITION by modulating pH gradient: when overactivated, they stimulate tumor

progression, while when inhibited they promote cancer cell death. CANCER CELL DEATH