What is the evidence that invasive species are a significant contributor to the decline or loss of threatened species? Philip D. Roberts1* (
[email protected]), Hilda Diaz-Soltero2 (
[email protected]), David J. Hemming1 (
[email protected]), Martin J. Parr1 (
[email protected]), Richard H. Shaw3 (
[email protected]), Nicola Wakefield1 (
[email protected]), Holly J. Wright1 (
[email protected]), and Arne B.R. Witt4 (
[email protected]). Abstract Background: The Convention on Biological Diversity has reported invasive species as 2nd greatest cause of species extinction (COP10). However few efforts have been made to collate the evidence to support or contest the impact of invasive species on the decline and/or extinction of threatened species across large taxonomic or geographical scales. This Systematic Review was commissioned by the United States Department for Agriculture (USDA) Invasives Causing Extinction (ICE) programme to determine if the COP10 statement was based on scientific evidence. The evidence needs to be systematically reviewed and mapped to determine the importance and relevance of any such effects in order to develop national and international policies addressing the loss of threatened species, and to prioritise research and mitigation efforts. Methods/design: The searching of online publication databases, grey literature and other resources, such as recovery plans of endangered species, aims to gather existing evidence on whether invasive species are a significant contributor to the decline and/or extinction of threatened