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The Role of Human Spliceosomal Component Srm300 in Neurodegenerative Disease

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The Role of Human Spliceosomal Component Srm300 in Neurodegenerative Disease THE ROLE OF HUMAN SPLICEOSOMAL COMPONENT SRM300 IN NEURODEGENERATIVE DISEASE RESPONSE TO SEROTONIN TREATMENT by GABRIELA ANA HORVATH MD, Institute of Medicine and Pharmacology Tirgu Mures, Romania, 1986 A THESIS SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY in THE FACULTY OF GRADUATE AND POSTDOCTORAL STUDIES (Experimental Medicine) THE UNIVERSITY OF BRITISH COLUMBIA (Vancouver) April 2015 © Gabriela Ana Horvath, 2015 Abstract Background Genetic research in neurodegenerative disorders identified candidate genes in many familial cases, providing insight into the pathogenesis. Many of them share common pathologic mechanisms which may result from alterations in gene expression. Single gene disorders can be great models for complex neurodegenerative pathophysiology, however, questions remain, and progress in their treatment is still a challenge. This research project started in the clinic, with a family with an apparently genetic neurodegenerative disorder, including hemiplegic migraines and systemic serotonin deficiency. Serotonin has roles in physiological and behavioural functions, neurodevelopment, as well as neuropsychiatric, gastrointestinal and cardiovascular disorders and migraines. Serotonin metabolism includes synthesis, transport and degradation. Methods Building hypothesis: started with translational research, taking basic science into practical applications. To answer the question of the systemic serotonin deficiency, genes of serotonin synthesis, transport and developmental pathway were sequenced, and platelet serotonin uptake studies were performed. Furthermore, SERT protein, actin and other cytoskeleton proteins were quantified with Western blots and proteomic analysis, in different platelet fractions. Generating hypothesis: continued with Whole Exome Sequencing. For validation, transcriptome analysis was performed. Along with investigations, treatment with serotonin replacement, 5-hydroxytryptophan was started in the patients. Results Sequencing was negative for genes associated with serotonin production, transport, and development. Serotonin uptake was deficient in patient platelets. Western blots showed diminished cytoskeleton proteins in Triton-soluble platelet fractions. WES analysis yielded a candidate gene, SRRM2, a splicing coactivator protein with potentially damaging missense mutation. Transcriptome analysis revealed several differentially expressed genes. Treatment with serotonin replacement significantly improved the lower limb strength. ii Conclusions Cytoskeleton proteins were aggregated trapping membrane proteins inside the cell, explaining the biochemical phenotype. The transcriptome analysis demonstrated significant changes in transcription levels in multiple ontology pathways. Some of these genes are known to cause neurodegenerative diseases. The changes in the transcription levels suggest that a defect at the core of the spliceosome complex is a reasonable hypothesis explaining the basis of this family’s neurodegeneration. The success with serotonin treatment is also new and deserves further research. iii Preface Choosing Experimental Medicine at UBC as my graduate program seemed to be the perfect fit for a research incorporating equally clinical and laboratory work. This choice was done together with my supervisory committee. All laboratory experiments presented in this thesis were designed, performed and interpreted by me, with the supervision of my advisors. A few of the Western blots were performed by the Nashville Lab, but later repeated by myself, and this is stated clearly in the thesis. The MRI’s and pathology slides were performed on clinical basis. The transcriptome analysis was interpreted also by me, based on information from various available webinars. The clinical case description and platelet serotonin uptake studies were published in the journal Cephalalgia (G Horvath, K Selby, K Poskitt, K Hyland, P Waters, M Coulter-Mackie, S Stockler-Ipsiroglu, Hemiplegic migraine, seizures, progressive spastic paraparesis, mood disorder, and coma in siblings with low systemic serotonin. Cephalalgia 31(15) 1580–1586). I wrote the case report and I did perform the platelet serotonin uptake analysis. I also made the graph with the serotonin uptake that has been published and appears in Chapter 3 (Figure 21). Dr. Selby contributed with clinical care, Dr. Poskitt reviewed the MRI’s, Dr. Hyland performed the CSF neurotransmitter analysis, and Dr. Coulter-Mackie, Waters and Stockler-Ipsiroglu are my supervisors. They supervised the experiments and helped with editing the manuscript. All authors read and approved the published paper. There are two manuscripts in preparation, the first is incorporating the results of the cytoskeleton experiments and transcriptome analysis, and it is written in collaboration with the Blakely Lab; the second is presenting the anatomical pathology findings on the autopsy, written in collaboration with Dr. Wayne Moore, Neuropathologist at Vancouver General Hospital and Dr. Peter Schutz, senior Pathology Resident. The results of this research have been presented at the Clinician Investigator and Experimental Medicine Programs Student Research days, and as oral presentations at several National and International meetings and conferences (at the 12th International Congress of Inborn Errors of Metabolism in 2013, at a Movement Disorders in Childhood meeting in Rome in 2013, and at the Canadian College of Medical Geneticists 39th Annual Scientific Conference in 2014). There were several poster presentations at the Child and Family Research Institute Student Research days. iv All experiments were conducted in the Marion Coulter-Mackie and Blakely Labs, with the University of British Columbia Ethics Board’s approval (Certificate # H07-02288). v Table of Contents Abstract .................................................................................................................................................... ii Preface ..................................................................................................................................................... iv Table of Contents ................................................................................................................................. vi List of Tables ......................................................................................................................................... xi List of Figures ..................................................................................................................................... xiii List of Illustrations ............................................................................................................................. xv List of Abbreviations ....................................................................................................................... xvi Acknowledgements ........................................................................................................................ xviii Dedication ............................................................................................................................................. xx Chapter 1: Background ................................................................................................................................. 1 1.1 Neurodegeneration .......................................................................................................................................... 2 1.2 Migraines .............................................................................................................................................................. 4 1.2.1 Familial hemiplegic migraine ................................................................................................................................. 5 1.2.2 Migraine with aura ...................................................................................................................................................... 5 1.2.3 Cortical spreading depression ............................................................................................................................... 6 1.2.4 Migraine treatment ..................................................................................................................................................... 7 1.3 Neuroanatomy ................................................................................................................................................... 8 1.3.1 Dorsal column-medial meniscus system ........................................................................................................... 8 1.3.2 Anterolateral system .................................................................................................................................................. 8 1.3.3 Corticospinal tracts ..................................................................................................................................................... 9 1.4 Neurotransmitters ........................................................................................................................................ 10 1.4.1 Neurotransmitter disorders ................................................................................................................................. 11 1.4.2 Serotonin ......................................................................................................................................................................
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