In Vitro Cell.Dev.Biol.—Animal DOI 10.1007/s11626-013-9614-4 2013 IN VITRO BIOLOGY MEETING ABSTRACT ISSUE Plant Posters P-2000 time-lapse videos capture. The use of gravity as a driving force for TIB operation has allowed the decoupling of gas and liquid Biosynthesis Enhancement of Stilbenoids in Hairy Root Cul- flow rates, making gas phase supplementation feasible, thus tures of Peanut. L. OGUTU, L. Nopo-Olazabal, and F. taking full advantage of the high mass-transfer coefficients Medina-Bolivar. Arkansas Biosciences Institute and Depart- achieved in these systems. Applications of this system include ment of Biological Sciences, Arkansas State University, supplementing O2 to improve growth rates and supplementing Jonesboro, AR. Email:
[email protected], CO2 in the development of sugar-free photoautotrophic bioreac-
[email protected] tors. Preliminary results growing N. benthamiana hairy root cultures in oxygen supplemented environments have shown that Resveratrol, a stilbenoid found in plants such as peanuts and these cultures are capable of utilizing a greater degree of available grapes, has a wide range of positive biological effects. Of sugars than controls. While experiments have shown comparable particular interest for this study are the hairy root cultures of amounts of tissue, this is believed to be caused by unequal peanut which have the ability to produce resveratrol and its stresses on each treatment due to periodic flow problems during prenylated analogs arachidin-1 and arachidin-3 upon elicitor prototype trouble-shooting. Photoautotrophic growth in the bio- treatment. Arachidin-1 has demonstrated higher antioxidant ac- reactor has also been demonstrated using seedless watermelon tivity and potentially higher metabolic stability than resveratrol tissue in elevated CO2 environments.