hydroxylatedand deaminated derivatives. liver for later use. IV Methadone acts more like heroin. In most states you must go to a pain clinic The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when or a Methadone maintenance clinic to be prescribed Methadone. Methadone is a long acting pain in urine exceed 1,000 ng/mL. This is the suggested screening cut-off for positive specimens set by reliever producing effects that last from twelve to forty-eight hours. Ideally, Methadone frees the the Substance Abuse and Mental Health Services Administration (SAMHSA, USA). 3 client from the pressures of obtaining illegal heroin, from the dangers of injection, and from the emotional roller coaster that most opiates produce. Methadone, if taken for long periods and at large doses, can lead to a very long withdrawal period. The withdrawals from Methadone are more One Step Drug Test BARBITURATES (BAR) prolonged and troublesome than those provoked by heroin cessation, yet the substitution and Barbiturates are central nervous system depressants. They are used therapeutically as sedatives, phased removal of methadone is an acceptable method of detoxification for patients and hypnotics, and anticonvulsants. Barbiturates are almost always taken orally as capsules or tablets. therapists.4 The effects resemble those of intoxication with alcohol. Chronic use of barbiturates leads to The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Methadone Package Insert for Multi Drug Screen Test Dipcard and Cassette tolerance and physical dependence. Short acting Barbiturates taken at 400 mg/day for 2-3 months in urine exceeds 300 ng/mL. can produce a clinically significant degree of physical dependence. Withdrawal symptoms This Instruction Sheet is for testing of any combination of the following drugs: experienced during periods of drug abstinence can be severe enough to cause death. Only a (mAMP) AMP/BAR/BZO/COC/THC/MTD/mAMP/MDMA/MOR/OPI/OXY/PCP/TCA small amount (less than 5%) of most Barbiturates are excreted unaltered in the urine. Methamphetamine is an addictive drug that strongly activates certain systems in the Including Adulterant Tests (Specimen Validity Tests) for: The approximate detection time limits for Barbiturates are: brain. Methamphetamine is closely related chemically to , but the central nervous Oxidants (OX), Specific Gravity (S.G) and pH. Short acting (e.g. Secobarbital) 100 mg PO (oral) 4.5 days system effects of Methamphetamine are greater. Methamphetamine is made in illegal laboratories Long acting (e.g. Phenobarbital) 400 mg PO (oral) 7 days4 and has a high potential for abuse and dependence. The drug can be taken orally, injected, or A rapid, one step screening test for the simultaneous, qualitative detection of multiple drugs and drug The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Barbiturates inhaled. Acute higher doses lead to enhanced stimulation of the central nervous system and Metabolites in human urine. in urine exceed 300 ng/mL. induce euphoria, alertness, reduced appetite, and a sense of increased energy and power. For Professional and In Vitro Diagnostic Use Only. Cardiovascular responses to Methamphetamine include increased blood pressure and cardiac BENZODIAZEPINES (BZO) arrhythmias. More acute responses produce anxiety, paranoia, hallucinations, psychotic behavior, INTENDED USE Benzodiazepines are medications that are frequently prescribed for the symptomatic treatment of and eventually, depression and exhaustion. The effects of Methamphetamine generally last 2-4 The STAT™ One Step Drug Test Dipcard/Cassette is a lateral flow chromatographic immunoassay anxiety and sleep disorders. They produce their effects via specific receptors involving a hours and the drug has a half-life of 9-24 hours in the body. Methamphetamine is excreted in the for the qualitative detection of multiple drugs and drug metabolites in urine at the following cut-off neurochemical called gamma aminobutyric acid (GABA). Because they are safer and more urine as amphetamine and oxidized and delaminated derivatives. However, 10-20% of concentrations: effective, Benzodiazepines have replaced barbiturates in the treatment of both anxiety and Methamphetamine is excreted unchanged. Thus, the presence of the parent compound in the insomnia. Benzodiazepines are also used as sedatives before some surgical and medical urine indicates Methamphetamine use. Methamphetamine is generally detectable in the urine for Test Calibrator Cut-off procedures, and for the treatment of seizure disorders and alcohol withdrawal. 3-5 days, depending on urine pH level. Amphetamine(AMP) D-Amphetamine 1,000 ng/mL Risk of physical dependence increases if Benzodiazepines are taken regularly (e.g., daily) for The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Barbiturates(BAR) Secobarbital 300 ng/mL more than a few months, especially at higher than normal doses. Stopping abruptly can bring on Methamphetamine in urine exceeds 1,000 ng/mL. Benzodiazepines(BZO) Oxazepam 300 ng/mL such symptoms as trouble sleeping, gastrointestinal upset, feeling unwell, loss of appetite, sweating, trembling, weakness, anxiety and changes in perception. (COC) Benzoylecgonine 300 ng/mL METHYLENEDIOXYMETHAMPHETAMINE (MDMA) Only trace amounts (less than 1%) of most Benzodiazepines are excreted unaltered in the urine; Methylenedioxymethamphetamine (ecstasy) is a designer drug first synthesized in 1914 by a Marijuana(THC) 11-nor-∆9-THC-9 COOH 50 ng/mL most of the concentration in urine is conjugated drug. The detection period for the German drug company for the treatment of obesity.8 Those who take the drug frequently report Methadone(MTD) Methadone 300 ng/mL Benzodiazepines in the urine is 3-7 days. adverse effects, such as increased muscle tension and sweating. MDMA is not clearly a stimulant, Methamphetamine(mAMP) D-Methamphetamine 1,000 ng/mL The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the although it has, in common with amphetamine drugs, a capacity to increase blood pressure and Methylenedioxymethamphetamine(MDMA) D,L-Methylenedioxymethamphetamine 500 ng/mL Benzodiazepines in urine exceed 300 ng/mL. heart rate. MDMA does produce some perceptual changes in the form of increased sensitivity to Opiate 300 (OPI 300,MOP,MOR) Morphine 300 ng/mL light, difficulty in focusing, and blurred vision in some users. Its mechanism of action is thought to Opiate 2000 (OPI 2000) Morphine 2,000 ng/mL COCAINE (COC) be via release of the serotonin. MDMA may also release , although the Oxycodone (OXY) Oxycodone 100 ng/mL Cocaine is a potent central nervous system (CNS) stimulant and a local anesthetic. Initially, it general opinion is that this is a secondary effect of the drug (Nichols and Oberlender, 1990). The brings about extreme energy and restlessness while gradually resulting in tremors, over-sensitivity most pervasive effect of MDMA, occurring in virtually all people who took a reasonable dose of the (PCP) Phencyclidine 25 ng/mL and spasms. In large amounts, cocaine causes fever, unresponsiveness, difficulty in breathing and drug, was to produce a clenching of the jaws. Tricyclic (TCA) Nortriptyline 1,000 ng/mL unconsciousness. The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Cocaine is often self-administered by nasal inhalation, intravenous injection and free-base Methylenedioxymethamphetamine in urine exceeds 500 ng/mL. Configurations of the STAT™ One Step Drug Test Dipcard/Cassette can consist of any combination of smoking. It is excreted in the urine in a short time primarily as Benzoylecgonine.1.2 the above listed drug analytes. This assay provides only a preliminary qualitative test result. Use a more specific Benzoylecgonine, a major metabolite of cocaine, has a longer biological half-life (5-8 hours) than OPIATE (OPI 300,MOP,MOR) alternate quantitative analytical method to obtain a confirmed analytical result. Gas chromatography/mass cocaine (0.5-1.5 hours), and can generally be detected for 24-48 hours after cocaine exposure.2 Opiate refers to any drug that is derived from the opium poppy, including the natural products, spectrometry (GC/MS) is the preferred confirmatory method.1 Apply clinical and professional judgment to any drug The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the cocaine morphine and codeine, and the semi-synthetic drugs such as heroin. Opioid is more general, of abuse test result, particularly when preliminary positive results are obtained. metabolite in urine exceeds 300 ng/mL. This is the suggested screening cut-off for positive referring to any drug that acts on the opioid receptor. specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, Opioid analgesics comprise a large group of substances which control pain by depressing the SUMMARY AND EXPLANATION OF THE TEST USA). 3 central nervous system. Large doses of morphine can produce higher tolerance levels, physiological dependency in users, and may lead to substance abuse. Morphine is excreted The STAT™ One Step Drug Test Dipcard/Cassette is a competitive immunoassay utilizing highly MARIJUANA (THC) unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine is specific reactions between antibodies and antigens for the detection of multiple drugs and drug THC (∆9-tetrahydrocannabinol) is the primary active ingredient in cannabis (marijuana). When detectable in the urine for several days after an opiate dose.4 metabolites in human urine. smoked or orally administered, THC produces euphoric effects. Users have impaired short term The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the The STAT™ One Step Drug Test Dipcard/Cassette is a rapid urine screening test that utilizes memory and slowed learning. They may also experience transient episodes of confusion and concentration of opiate exceeds the 300 ng/mL cut-off level. monoclonal antibodies to selectively detect elevated levels of specific drugs in urine without the use of an anxiety. Long-term, relatively heavy use may be associated with behavioral disorders. The peak instrument. effect of marijuana administered by smoking occurs in 20-30 minutes and the duration is 90-120 OPIATE (OPI 2000) minutes after one cigarette. Elevated levels of urinary metabolites are found within hours of Opiate refers to any drug that is derived from the opium poppy, including the natural products, AMPHETAMINE(AMP) exposure and remain detectable for 3-10 days after smoking. The main metabolite excreted in the morphine and codeine, and the semi-synthetic drugs such as heroin. Opioid is more general, Amphetamine is a Schedule II controlled substance available by prescription (Dexedrine®) and is urine is 11-nor-∆9-tetrahydrocannabinol-9-carboxylic acid ( ∆9-THC-COOH). referring to any drug that acts on the opioid receptor. also available on the illicit market. Amphetamines are a class of potent sympathomimetic agents The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Opioid analgesics comprise a large group of substances which control pain by depressing the with therapeutic applications. They are chemically related to the human body's natural concentration of THC-COOH in urine exceeds 50 ng/mL. This is the suggested screening cut-off central nervous system. Large doses of morphine can produce higher tolerance levels, : epinephrine and . Acute higher doses lead to enhanced for positive specimens set by the Substance Abuse and Mental Health Services Administration physiological dependency in users, and may lead to substance abuse. Morphine is excreted PN: Y0311110902 stimulation of the central nervous system and induce euphoria, alertness, reduced appetite, and a (SAMHSA, USA). 3 unmetabolized, and is also the major metabolic product of codeine and heroin. Morphine is sense of increased energy and power. Cardiovascular responses to Amphetamines include detectable in the urine for several days after an opiate dose.3 increased blood pressure and cardiac arrhythmias. More acute responses produce anxiety, METHADONE (MTD) The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the morphine in paranoia, hallucinations, and psychotic behavior. The effects of Amphetamines generally last 2-4 Methadone is a narcotic analgesic prescribed for the management of moderate to severe pain and urine exceeds 2,000 ng/mL. This is the suggested screening cut-off for positive specimens set by hours following use, and the drug has a half-life of 4-24 hours in the body. About 30% of for the treatment of opiate dependence (heroin, Vicodin, Percocet, Morphine). The pharmacology the Substance Abuse and Mental Health Services Administration (SAMHSA, USA). Amphetamines are excreted in the urine in unchanged form, with the remainder as of Oral Methadone is very different from IV Methadone. Oral Methadone is partially stored in the OXYCODONE (OXY) competitive binding. Drugs which may be present in the urine specimen compete against their DIRECTIONS FOR USE Oxycodone,[4,5-epoxy-14-hydroxy-3-methoxy-17-methyl-morphinan-6-one, respective drug conjugate for binding sites on their specific antibody. dihydrohydroxycodeinone] is a semi-synthetic opioid agonist derived from thebaine, a constituent Allow the test device to come to room temperature [15-30oC (59-86oF)] prior to testing. of opium. Oxycodone is a Schedule II narcotic analgesic and is widely used in clinical medicine. During testing, a urine specimen migrates upward by capillary action. A drug, if present in the urine The pharmacology of oxycodone is similar to that of morphine, in all respects, including its abuse specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. [For Dip Card] and dependence liabilities. Pharmacological effects include analgesia, euphoria, feelings of The antibody will then react with the drug-protein conjugate and a visible colored line will show up relaxation, respiratory depression, constipation, papillary constriction, and cough suppression. in the test line region of the specific drug strip. The presence of drug above the cut-off (1) Remove the test device from its foil wrapper by tearing along the slit (bring the container to the Oxycodone is prescribed for the relief of moderate to high pain under pharmaceutical trade names concentration will saturate all the binding sites of the antibody. Therefore, the colored line will not room temperature before opening to avoid condensation of moisture in container). Label the as OxyContin® (controlled release), OxyIR®, OxyFast®(immediate release formulations), or form in the test line region. device with patient or control identifications. Percodan® (aspirin) and Percocet® (acetaminophen) that are in combination with other (2) Remove the cap from the test device. Label the device with donor or control identifications. nonnarcotic analgesics. Oxycodone's behavioral effects can last up to 5 hours. The A drug-positive urine specimen will not generate a colored line in the specific test line region of the (3) Immerse the absorbent tip into the urine sample about 10-15 seconds until the migration of controlled-release product, OxyContin®, has a longer duration of action (8-12 hours). strip because of drug competition, while a drug-negative urine specimen will generate a line in the urine fluid is observed in the result windows. Urine sample should not touch the plastic device. The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Oxycodone test line region because of the absence of drug competition. (4) Replace the cap over the absorbent tip and lay the device flatly on a non-absorptive clean in urine exceeds 100 ng/mL. surface. Start the timer. To serve as a procedural control, a colored line will always appear at the control line region, (5) Read results at 5 minutes. DO NOT INTERPRET RESULT AFTER 10 MINUTES. PHENCYCLIDINE (PCP) indicating that proper volume of specimen has been added and membrane wicking has occurred. Phencyclidine, also known as PCP or Angel Dust, is a hallucinogen that was first marketed as a surgical anesthetic in the 1950's. It was removed from the market because patients receiving it REAGENTS became delirious and experienced hallucinations. Phencyclidine is used in powder, capsule, and tablet form. The powder is either snorted or smoked The test contains a membrane strip coated with drug-protein conjugates (purified bovine albumin) after mixing it with marijuana or vegetable matter. Phencyclidine is most commonly administered on the test line, a goat polyclonal antibody against gold-protein conjugate at the control line, and a by inhalation but can be used intravenously, intra-nasally, and orally. After low doses, the user dye pad which contains colloidal gold particles coated with mouse monoclonal antibody specific to thinks and acts swiftly and experiences mood swings from euphoria to depression. Self-injurious Amphetamine, Cocaine, Methamphetamine, Methylenedioxymethamphetamine, Morphine, THC, behavior is one of the devastating effects of Phencyclidine. Phencyclidine, Benzodiazepines, Methadone, Barbiturates, Tricyclic Antidepressants or [For Cassette] PCP can be found in urine within 4 to 6 hours after use and will remain in urine for 7 to 14 days, Oxycodone. depending on factors such as metabolic rate, user's age, weight, activity, and diet.5 Phencyclidine (1) Remove the test device from its foil wrapper by tearing along the slit (bring the container to the is excreted in the urine as an unchanged drug (4% to 19%) and conjugated metabolites (25% to ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) REAGENTS room temperature before opening to avoid condensation of moisture in container). Label the 30%).6 device with donor or control identifications. The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the Adulteration Pad Reactive Indicator Buffers and Non-reactive Ingredients (2) Using the specimen dropper, withdraw the urine sample from the specimen collection container phencyclidine level in urine exceeds 25 ng/mL. This is the suggested screening cut-off for positive Oxidants (OX) 0.36% 99.64% and slowly dispense 3~4 drops (approximately 120mL) into the sample wells (S), being careful specimens set by the Substance Abuse and Mental Health Services Administration (SAMHSA, Specific Gravity (S.G.) 0.25% 99.75% not to overfill the absorbent pad. Start the timer. USA). pH 0.06% 99.94% (3) Read results at 5 minutes.DO NOT INTERPRET RESULT AFTER 10 MINUTES.

TRICYCLIC ANTIDEPRESSANTS (TCA) PRECAUTIONS TCA (Tricyclic Antidepressants) are commonly used for the treatment of depressive disorders. TCA overdoses can result in profound central nervous system depression, cardiotoxicity and • For Professional Use Only. anticholinergic effects. TCA overdose is the most common cause of death from prescription drugs. • For In Vitro Diagnostic Use Only. TCAs are taken orally or sometimes by injection. TCAs are metabolized in the liver. Both TCAs • Do not use after the expiration date. and their metabolites are excreted in urine mostly in the form of metabolites for up to ten days. • The test panel should remain in the sealed pouch until use. The STAT™ One Step Drug Test Dipcard/Cassette yields a positive result when the • The test is for single use. concentration of Tricyclic Antidepressants in urine exceeds 1,000 ng/mL. • While urine is not classified by OSHA or the CDC as a biological hazard unless visibly contaminated with blood8,9, the use of gloves is recommended to avoid unnecessary contact ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) SUMMARY with the specimen. • The used test card and urine specimen should be discarded according to federal, state and local C C C The Adulterant Test Strip contains chemically treated reagent pads. Observation of the color regulations. T T T change on the strip compared to the color chart provides a semi-quantitative screen for oxidants, specific gravity and pH in human urine which can help to assess the integrity of the urine STORAGTE AND STABILITY NEGATIVE POSITIVE INVALID specimen. Store as packaged in the sealed pouch at 2-30°C (36-86°F). The test is stable through the ADULTERATION expiration date printed on the sealed pouch. The test device must remain in the sealed pouch until INTERPRETATION OF RESULTS use. DO NOT FREEZE. Do not use beyond the expiration date. Adulteration is the tampering of a urine specimen with the intention of altering the test results. The (Please refer to the previous illustration) use of adulterants in the urine specimen can cause false negative results by either interfering with SPECIMEN COLLECTION AND PREPARATION NEGATIVE: Two lines appear. * One color line should be in the control region (C), and another the test and/or destroying the drugs present in the urine. Dilution may also be used to produce apparent color line adjacent should be in the test region (T). This negative result indicates that the false negative drug test results. To determine certain urinary characteristics such as specific Urine Assay drug concentration is below the detectable level. gravity and pH, and to detect the presence of oxidants in urine are considered to be the best ways The urine specimen should be collected directly into the test cup. Urine collected at any time of the *NOTE: The shade of color in the test line region (T) will vary, but it should be considered negative to test for adulteration or dilution. day may be used. If the urine specimen is collected for later testing, another dry and clean whenever there is even a faint distinguishable color line. • Oxidants (OX): Tests for the presence of oxidizing agents such as bleach and peroxide in the container should be used to collect the specimen. urine. Specimen Storage POSITIVE: One color line appears in the control region (C). No line appears in the test region (T). • Specific Gravity (S.G.): Tests for sample dilution. Normal levels for specific gravity will range Urine specimens may be stored at 2-8°C (36-46°F) for up to 48 hours prior to testing. For This positive result indicates that the drug concentration is above the detectable level. from 1.003 to 1.030. Specific gravity5 levels of less than 1.003 or higher than 1.030 may be an prolonged storage, specimens may be frozen and stored below -20°C. Frozen specimens should indication of adulteration or specimen dilution. be thawed and mixed well before testing. INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural • pH: tests for the presence of acidic or alkaline adulterants in urine. Normal pH levels should be techniques are the most likely reasons for control line failure. Review the procedure and repeat in the range of 4.0 to 9.0. Values below pH 4.0 or above pH 9.0 may indicate the sample has MATERIALS the test using a new test device. If the problem persists, discontinue using the lot immediately and been altered. Materials Provided contact your supplier. • Test device • Desiccants • Dropper (for cassette) • Package insert PRINCIPLE • Color Chart Card for Adulterant Interpretation (when applicable) ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) INTEPRETATION Materials Required But Not Provided The STAT™ One Step Drug Test Dipcard/Cassette is an immunoassay based on the principle of · Specimen collection container · Disposable gloves · Timer (Please refer to the color chart) Semi-quantitative results are obtained by visually comparing the reacted color blocks on the strip to the printed color indicator on the color chart. No instrumentation is required. % Agreement with GC/MS (HPLC for TCA) Cocaine(COC) AMP mAMP OPI 2000 OPI 300 COC PCP THC Benzoylecgonine Total number of Result Precision Positive conc.(ng/mL) Determinations ADULTERANT TESTS (SPECIMEN VALIDITY TESTS) LIMITATIONS 95% 96% >99% 96% 96% 95% 96% Agreement No drug present 40 40 negative >99% Negative >99% >99% 97% >99% >99% >99% >99% 1. The adulterant tests included with the product are meant to aid in the determination of abnormal Agreement 150 40 40 negative >99% Overall 225 40 40 negative >99% specimens, but may not cover all the possible adulterants. Agreement 98% 98% 98% 98% 98% 95% 98% 2. Oxidants: Normal human urine should not contain oxidants. The presence of high level of 375 40 40 positive >99% antioxidants in the specimen, such as ascorbic acid, may result in false negative results for the 450 40 40 positive >99% oxidants pad.. BAR TCA MDMA BZO MTD OXY 3. Specific Gravity: Elevated levels of protein in urine may cause abnormally high specific gravity Positive Agreement 97% 98% 93% 96% 94% 95% values. Negative Agreement 98% >99% >99% >99% 98% >99% Marijuana(THC) QUALITY CONTROL Overall 9 Agreement 98% 99% 96% 98% 96% 98% 11-nor-∆ -THC-9-COOH Total number of Result Precision conc.(ng/mL) Determinations A procedural control is included in the test. A color line appearing in the control region (C) is BAR MDMA BZO MTD OXY TCA THC PCP mAMP OPI300 OPI2000 COC AMP No drug present 40 40 negative >99% considered an internal procedural control. It confirms sufficient specimen volume, adequate Analy te Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg Pos Neg 25 40 40 negative >99% membrane wicking and correct procedural technique. Negativ e Samples 0 4 0 4 0 5 0 3 0 4 0 4 0 0 0 1 0 4 0 3 0 17 0 0 0 0 Near Cut-of f Negativ e 37.5 40 40 negative >99% Samples [between 50% 1 37 0 36 0 28 1 44 0 36 0 36 0 15 0 0 0 10 0 11 1 13 0 13 0 19 LIMITATIONS of cut-of f and cut-of f ] 50 40 40 positive >99% Near Cut-of f Positiv e Samples [between cut- 34 1 33 3 27 2 27 2 34 2 35 1 23 1 7 2 3 1 18 1 3 0 26 1 7 1 75 40 40 positive >99% 1. The STAT™ One Step Drug Test Dipcard/Cassette provides only a qualitative, preliminary of f and 150% of cut- Positiv e Samples 3 0 4 0 18 0 3 0 4 0 4 0 1 0 28 0 22 0 7 0 6 0 0 0 13 0 analytical result. A secondary analytical method must be used to obtain a confirmed result. Gas [>150% of cut-of f ] chromatography/mass spectrometry (GC/MS) is the preferred confirmatory method. 3,4,7 Agreement with GC/MS 97% 98% 93% >99% 96% >99% 94% 98% 95% >99% 98% >99% 96% >99% 95% >99% 96% >99% 96% >99% >99% 97% 96% >99% 95% >99% 2. There is a possibility that technical or procedural errors, as well as other interfering substances Methadone(MTD) in the urine specimen may cause erroneous results. Reproducibility 3. Adulterants, such as bleach and/or alum, in urine specimens may produce erroneous results Reproducibility studies were carried out using commercially available standards. Each standard Methadone Total number of Result Precision regardless of the analytical method used. If adulteration is suspected, the test should be was diluted in normal, drug-free urine to give the appropriate concentration. Each specimen, at conc.(ng/mL) Determinations repeated with another urine specimen and a new test device. each concentration of analyte, was tested four times daily, in duplicate, for five consecutive days. A No drug present 40 40 negative >99% 4. A Positive result does not indicate intoxication of the donor, the concentration of drug in the total of 40 determinations were made at each concentration. The results are given below: 150 40 40 negative >99% urine, or the route of drug administration. 225 40 40 negative >99% 5. A Negative result may not necessarily indicate drug-free urine. Negative results can be 300 40 40 positive >99% obtained when drug is present but below the cut-off level of the test. 6. Test does not distinguish between drugs of abuse and certain medications. Amphetamine(AMP) 450 40 40 positive >99% 7. A positive test result may be obtained from certain foods or food supplements. Amphetamine(AMP) Total number of Result Precision conc.(ng/mL) Determinations PERFORMANCE CHARACTERISTICS No drug present 40 40 negative >99% Methamphetamine(mAMP) Accuracy 500 40 40 negative >99% 750 40 40 negative >99% Methamphetamine Total number of Result Precision conc.(ng/mL) Determinations Testing on accuracy of the test strips was performed on clinical specimens collected for each of 1,000 40 40 positive >99% the following drug types. All clinical specimens were quantified by GC/MS analysis before testing. 1,500 40 40 positive >99% No drug present 40 40 negative >99% The quantity of the following compounds were analyzed by GC/MS and contributed to the total 500 40 40 negative >99% amount of drugs found in the positive specimens tested. 750 40 40 negative >99% Barbiturates(BAR) 1,000 40 40 positive >99% Test Compounds Contributed to the Totals of GC/MS Secobarbital Total number of Result Precision 1,500 40 40 positive >99% AMP Amphetamine conc.(ng/mL) Determinations BAR Secobarbital No drug present 40 40 negative >99% BZO Oxazepam 150 40 40 negative >99% COC Benzoylecgornine 225 40 40 negative >99% Methylenedioxymethamphetamine(MDMA) THC 11-nor-∆9-tetrahydrocannabinol-9-carboxylic acid 300 40 40 positive >99% Methylenedioxymeth- Total number of Result Precision MTD Methadone 450 40 40 positive >99% amphetamine conc.(ng/mL) Determinations mAMP Methamphetamine No drug present 40 40 negative >99% Benzodiazepines(BZO) MDMA D,L-Methylenedioxymethamphetamine, Methylenedioxymethamphetamine 250 40 40 negative >99% OPI Morphine, Codeine Oxazepam Total number of Result Precision 375 40 40 negative >99% conc.(ng/mL) Determinations OXY Oxycodone 500 40 40 positive >99% PCP Phencyclidine No drug present 40 40 negative >99% 750 40 40 positive >99% TCA Nortriptyline 150 40 40 negative >99% 225 40 40 negative >99% The following results are tabulated from these clinical studies: 300 40 40 positive >99% PN: Y0311110902 450 40 40 positive >99% Chlordiazepoxide 1,565 Analytical Sensitivity Opiate 300 (OPI 300,MOP,MOR) Chlordiazepoxide HCl 780 A drug-free urine pool was spiked with drugs at concentrations listed. The results are Clobazam 100 Morphine Total number of Result Precision summarized below conc.(ng/mL) Determinations Clonazepam 785 No drug present 40 40 negative >99% Clorazepate Dipotassium 195 Drug concentration AMP BAR BZO COC Delorazepam 150 40 40 negative >99% n 1,560 Cut-off Range - + - + - + - + Desalkylflurazepam 225 40 40 negative >99% 390 0% Cut-off 10 10 0 10 0 10 0 10 0 Diazepam 300 40 40 positive >99% 195 Estazolam 2,500 375 40 40 positive >99% -50% Cut-off 10 10 0 10 0 10 0 10 0 Flunitrazepam -25% Cut-off 10 10 0 10 0 10 0 10 0 385 (±) Lorazepam 1,560 Cut-off 10 0 10 0 10 0 10 0 10 RS-Lorazepam glucuronide Opiate 2000 (OPI 2000) 160 +25% Cut-off 10 0 0 0 10 0 10 0 10 Midazolam 12,500 Morphine Total number of Result Precision +50% Cut-off 10 0 10 0 10 0 10 0 10 Nitrazepam 95 conc.(ng/mL) Determinations Norchlordiazepoxide 200 No drug present 40 40 negative >99% Nordiazepam 390 Drug concentration THC MTD mAMP MDMA MOP 1,000 40 40 negative >99% n Oxazepam 300 Cut-off Range - + - + - + - + - + 1,500 40 40 negative >99% Temazepam 100 0% Cut-off 10 10 0 10 0 10 0 10 0 10 0 2,000 40 40 positive >99% Triazolam 2,500 3,000 40 40 positive >99% -50% Cut-off 10 10 0 10 0 10 0 10 0 10 0 -25% Cut-off 10 10 0 10 0 10 0 10 0 10 0 COCAINE (COC) Benzoylecogonine 300 Cut-off 10 0 10 0 10 0 10 0 10 0 10 Oxycodone (OXY) Cocaethylene 300 +25% Cut-off 10 0 0 0 10 0 0 0 10 0 10 Cocaine 300 Nortiptyline Total number of Result Precision conc.(ng/mL) Determinations +50% Cut-off 10 0 10 0 10 0 10 0 10 0 0 Metoclopromide 80,000 Procaine 75,000 No drug present 40 40 negative >99%

50 40 40 negative >99% Drug concentration n OPI OXY PCP TCA MARIJUANA (THC) 75 40 40 negative >99% Cut-off Range - + - + - + - + 11-Nor-∆9-Tetrahydrocannabinol 50 100 40 40 positive >99% 0% Cut-off 10 10 0 10 0 10 0 10 0 11-Hydroxy-∆9-Tetrahydrocannabinol 5,000 150 40 40 positive >99% -50% Cut-off 10 10 0 10 0 10 0 10 0 11-Nor-∆8-Tetrahydrocannabinol 50 -25% Cut-off 10 10 0 10 0 10 0 10 0 11-Nor-∆9-Tetrahydrocannabinol-9 Carboxylic Glucuronide 2,500 ∆8-Tetrahydrocannabinol 20,000 Phencyclidine (PCP) Cut-off 10 0 10 0 10 0 10 0 10 ∆9 –Tetrahydrocannabinol +25% Cut-off 10 0 0 0 10 0 10 0 10 20,000 Phencyclidine Total number of Result Precision conc.(ng/mL) Determinations +50% Cut-off 10 0 10 0 10 0 0 0 10 METHADONE (MTD) No drug present 40 40 negative >99% Methadone 300 12.5 40 40 negative >99% Doxylamine 50,000 19 40 40 negative >99% Analytical Specificity The following table lists the concentration of compounds (ng/mL) that were detected positive in 25 40 40 positive >99% METHAMPHETAMINE (mAMP) urine by STAT™ One Step Drug Test Dipcard/Cassette at a read time of 5 minutes 37.5 40 40 positive >99% (+/-) 3,4-Methylenedioxy-n-ethylamphetamine(MDEA) 20,000 Procaine (Novocaine) 60,000 Drug Concentration(ng/ml) 20,000 ng/mL Tricyclic antidepressants (TCA) AMPHETAMINE (AMP) +/-methamphetamine 1,000 1,000 d-amphetamine +methamphetamine 500 Nortiptyline Total number of Result Precision D,l-amphetamine 1,000 conc.(ng/mL) Determinations Ranitidine (Zantac) 50,000 20,000 l-amphetamine (+/-) 3,4-Methylenedioxymethamphetamine (MDMA) 2,500 No drug present 40 40 negative >99% 1,250 500 40 40 negative >99% (+/-)- Methylenedioxyamphetamine (MDA) 1,500 METHYLENEDIOXYMETHAMPHETAMINE (MDMA) 750 40 40 negative >99% D,L-3,4-Methylenedioxymethamphetamine (MDMA) 500 1,000 40 40 positive >99% BARBITURATES (BAR) 3,4-Methylenedioxyamphetamine HCI (MDA) 3,000 1,500 40 40 positive >99% Secobarbital 300 3,4-Methylenedioxyethyl-amphetamine (MDEA) 300 Amobarbital 300 Alphenol 150 OPIATES (OPI 300,MOP,MOR) Aprobarbital 200 6-acetylmorphine 500 Butabarbital 75 Codeine 100 Butalbital 2,500 Eserine (Physosotigmine) 15,000 Butethal 100 Ethylmorphine 100 Cyclopentobarbital 600 Heroin 500 Pentobarbital 300 Hydromorphone 2,000 Phenobarbital 100 Hydrocodone 1,250 Morphine 300 BENZODIAZEPINE (BZO) Morphine-3-glucuronide 75 a-Hydroxyalprazolam 1,260 Oxycodone 75,000 Alprazalam 200 Thebaine 13,000 Bromazepam 1,560 Etodolac Prilocaine Chlorpropamide Drug Concentration(ng/ml) Acetaldehyde Etoposide Hydroxyprogesterone Primaquine Acetaminophen Famotidine Methylene Blue Primidone OPIATES (OPI 2000) Acetamidophenol( N-Acetyl-p-aminophenol ) Chlorthalidone (Ritalin ) Proadifen 6-acetylmorphine 1,000 Acetazolamide Chlorzoxazone Fenoprofen Methyl Salicylate Probenecid Codeine 800 Acetone Cholesterol Fentanyl Meticrane Procainamide Ethylmorphine 400 Acetophenetidin Cimetidine Ferrous Sulfate Metronidazole Acetopromazine Cinchonidine Flufenamic Acid Procyclidine Heroin 10,000 N-Acetyl-L-cysteine Cinoxacin Flunisolide Milrinone Hydromorphone 2,000 N-Acetylprocainamide (Acedainide ) Clemastine Minaprine Hydrocodone 5,000 Acetylsalicylic Acid ( Aspirin Flurandrenolide Nabumetone Propionylpromazine Morphine 2,000 Albumin, standard Clindamycin Flurazepam Protriptyline Allobarbital (Diallybarbituric Acid ) Clobetasone Butyrate Flurbiprofen Morphine-3-glucuronide 1,000 Nafcillin Allopurinol ( 4-Hydroxypyrazole(3,4- pyrimidine ) Formaldehyde Nalbuphine Pyridine-2-Aldoxime Oxycodone 50,000 Furosemide Nalidixic Acid Pyridoxine Thebaine 26,000 (Adamantan-1-amine ) Cloxacillin Gemfibrozil Nalmefene Pyrilamine Amcinonide Gentamicin Sulfate Nalorphine Colchicine Gentisic Acid Quinine OXYCODONE (OXY) Amikacin Naloxone Amiloride Cortisone Glucose Naltrexone Quinolinic Acid Oxycodone 100 p-Aminobenzoic Acid Cortol Glybenclamide - Ranitidine Codeine 50,000 DL-Aminoglutethimide Creatinine Griseofulvin Naphthalene Acetic Acid Rescinnamine Dihydrocodeine 12,500 Amiodarone Cromolyn Guaiacol Glyceryl Ether Naphthol Reserpine Cyclobenzaprine Guanethidine Ethylmorphine 25,000 Amitryptyline Neomycin Sulfate Riboflavin Ammonium Chloride Cyclophosphamide Halcinonide Niacinamide Hydrocodone 1,580 Cyclosporin A Nialamide (Albuterol ) Hydromorphone 12,500 Amphotericin B Hemoglobin (+/-) Nicotine Salicylic Acid Oxymorphone 1,580 Ampicillin Dantrolene Hexachlorocyclohexane Nicotinic Acid Sodium Chloride Deferoxamine Mesylate Thebaine 50,000 Aniline Hexachlorophene Nifedipine Sodium Formate Antipyrine Hexobarbital Nitrofurantoin Sulfamethazine Hippuric Acid Sulfamethoxazole PHENCYCLIDINE (PCP) L-Ascorbic Acid Desmethyldiazepam Histamine Norclomipramine Sulfanilamide Phencyclidine 25 ASP-PHE-Methyl-Ester ( Aspartame) Desoximetasone DL-Homatropine Sulfathiazole 4-Hydroxy PCP 90 D-Aspartic Acid Dexamethasone Hydrastine Norcodeine Sulfisoxazole DL-Aspartic Acid Dextromethorphan Hydrochlorothiazide Sulindac PCP Morpholine 625 Nordoxepin L–Aspartic Acid Diazoxide Hydrocortisone Norethindrone Talbutal Baclofen Dichloromethane Hydrocarbalamine Norfloxacin Tannic Acid TRICYCLIC ANTIDEPRESSANTS (TCA) Barbituric Acid Dichlorphenamide Hydroflumethiazide Normorphine Notriptyline 1,000 Beclomethasone Diclofenac Hydroxyhippuric Acid Noscapine Terfenadine Dicyclomine Tetracycline 1,500 Beclomethasone Dipropionate Nylidrin Bendroflumethiazide Dieldrin Ibuprofen Orphenadrine Theobromine Clomipramine 12,500 Benzidine Diflorasone Diacetate Indapamide Oxalic Acid Desipramine 200 Benzilic Acid diethylaminoethyl ester Diflucortolone pivalate Indomethacin Thiamine Doxepine 2,000 Benzocaine Diflunisal Ipratropium Bromide Digitoxin Iproniazid Tobramycin 400 Benzoic Acid Digoxin Isonicotinic Acid Oxyphenbutazone Tolazamide 2,000 Benzthiazide Dihydroxymandelic Acid Isopropamide Oxypurinol Tolbutamide Nordoxepine 1,000 Benztropine Theophylline Paclitaxel Tolmetin Promazine 1,500 Benzyl alcohol Dimenhydrinate Kanamycin Pancuronium Bromide Toluene Dimercaprol Promethazine 2,500 Benzylamine Papaverine Berberine Dimethylaminoantipyrin Ketoprofen Pargyline Triamcinolone 3,000 Betamethasone Dimethyl Isosorbide Kynurenic Acid Penicillin Triamterene Bilirubin Dimethyl Sulfoxide Pentachlorophenoll Trichlormethiazide Brompheniramine Diphenhydramine Levorphanol Pentoxifylline Trichloroacetic acid Dipyridamole Lidocaine Effect of Urinary Specific Gravity Bumetanide Pentylenetetrazole Dipyrone Lisinopril Fifteen (15) urine samples of normal, high, and low specific gravity ranges (1.005, 1.015, 1.03) were Bupivacaine p-Phenylenediamine Disopyramide Lithium Carbonate Trimethoprim Buprenorphine Phenelzine spiked with drugs at 50% below and 50% above cut-off levels respectively. The STAT™ One Step Loperamide Trimipramine Phenformin Drug Test Dipcard/Cassette was tested in duplicate using ten drug-free urine and spiked urine Lormetazepam Triprolidine Butacaine Pheniramine samples. The results demonstrate that varying ranges of urinary specific gravity do not affect the test Doxycycline Lysergic Acid Diethylamide (LSD) Tropic Acid Butyrophenone Phenol results. Doxylamine Mebendazole Tropine Phenolphthalien Meclizine Tryptamine Camphor Ecgonine Meclofenamic Acid Canrenoic Acid Phenoxymethyl Effect of the Urinary pH Ecgonine Methyl Ester Medazepam Urea ( Carbamide ) The pH of an aliquoted negative urine pool was adjusted to pH ranges of 4.0 ,4.5, 5.0, 6.0 and 9.0,and Captopril Penicillinic acid ( Penicillin V ) Emetine Mefenamic Acid Uric Acid Carbamazepine spiked with drugs at 50% below and 50% above cut-off levels. The spiked, pH-adjusted urine was Melanin Vancomycin Carbamyl-Carboplatin Phenylbutazone tested with the STAT™ One Step Drug Test Dipcard/Cassette . The results demonstrate that Epinephrine Melphalan Vincamine Carisoprodol Phenylethylamine varying ranges of pH do not interfere with the performance of the test. Erythromycin Menthol Cefaclor Eserine Meperidine Cefadroxil Phenyltoloxamine Estradial Mephenesin Zearalenone Cross-Reactivity Cefotaxime Picrotoxin Estriol Zomepirac A study was conducted to determine the cross-reactivity of the test with compounds in either drug-free Cefoxitin Pilocarpine Estron Meprobamate Zopiclone urine or drug positive urine containing Cocaine, Barbiturates, Benzodiazepines, Amphetamine, Ceftriaxone Glucuronide Metaproterenol Methamphetamine, Marijuana, Methadone, MDMA (Ecstasy), Opiates, Oxycodone, Phencyclidine or Cefuroxime Pinacidil Estrone-3-Sulfate Tricyclic Antidepressants. The following compounds show no cross-reactivity when tested with The Cephalexin Ethacrynic Acid Methadone Non Cross-Reacting Cephaloridine Pipecolic Acid PN: Y0311110902 STAT™ One Step Drug Test Dipcard/Cassette at concentrations of 100.µg/mL. Ethambutol Methanol, Absolute Pipedemic Acid Compound of Methadone Cephradine Ethamivan Methaqualone Chloramphenicol Piroxicam *Parent compound only： Ethanol, Standard Methazolamide Potassium Chloride Non Cross-Reacting Compounds Chlorcyclizine Ethopropazine Methotrimeprazine Chloroquine Potassium Iodide Cocaine, Benzodiazepines, Amphetamine, Methamphetamine, Marijuana, Opiates, Ethosuximide Phenylnalonamide Acebutolol Chlorothiazide Prazepam Oxycodone, Phencyclidine, Barbiturates Non Cross-Reacting Compound Ethylene Glycol Naphthalene Acetic Acid Acetaldehyde Chlorotrianisene *Parent compound only： Ethylenediamine Tetraacetic Acid Naproxen Prednisone Acetaminophen Acetazolamide Chlorpheniramine Methoxyamine Hydroxyhippuric Acid Acetone Probenecid 4-Acetamidophenol 3,4-Methylene-dioxyethylamphetamine amphotericin B prednisolone temazepam p-Hydroxyamphetamine Acetophenetidin Procainamide Acetophenetidin (+)3,4-Methylene-dioxymethamphetamine ampicillin prednisone tetracycline Hydroxyzine N-Acetylprocainamide (Acedainide ) Prochlorperazine N-Acetylprocainamide Methylphenidate amtriptyline prochloperazine tetrahydrocortisone Ibuprofen Acetylsalicylic Acid ( Aspirin) Procyclidine Acetylsalicylic acid Morphine-3-β-D-glucuronide apomorphine promethazine theophyline Imipramine Aminopyrine Promazine Aminopyrine Morphine sulfate ascorbic acid propoxyphen thiamine Indapamide Amitryptyline Promethazine Amobarbital Nalidixic acid aspartate thioridazine Indomethacin Ammonium Chloride Propionylpromazine Amoxicillin Naloxone aspirin prozac(fluoxetin) thyroxine Ipratropium Bromide Amobarbital Protriptyline DL-Amphetamine Naltrexone pseudoephedrine tobutamide Iproniazid Amoxicillin Pseudoephedrine Ampicillin Naproxen pyroxidine trazodone Isonicotinic Acid Amphotericin B Pyridine-2-Aldoxime Ascorbic acid Niacinamide beclomethasone quinidine trimethoprim Isopropamide Ampicillin Pyridoxine Apomorphine Nifedipine benzocaine ranitidine tryptophan Isoxsuprine Aniline Pyrilamine Aspartame Norcodein benzoic acid riboflavin Kanamycin Antipyrine Quinidine Atropine Norethindrone bilirubin salicylic acid urea Ketamine DL-Amphetamine sulfate Quinine Benzilic acid D-Norpropoxyphene sidenafil(viagra) uric acid Ketoprofen DL-Aspartic Acid Quinolinic Acid Benzoic acid Noscapine buspirone sodium chloride valproic acid Kynurenic Acid L–Aspartic Acid Oxazepam Benzoylecgonine D,L- caffeine sulfamethoxazole Labetalol Apomorphine Ranitidine Benzphetamine Oxalic acid captopril sulindac Zoloft Levorphanol Aprobarbital Rescinnamine Bilirubin Oxazepam carbamazepine Loperamide Aspartame Reserpine Brompheniramine Oxolinic acid cefaclor Meperidine Atropine Riboflavin Caffeine Oxycodone cemetidine Mephentermine Barbituric Acid Ritodrine Cannabidiol Oxymetazoline chloramphenicol Methoxyphenamine Cannabinol BIBLIOGRAPHY Benzidine Salbutamol (Albuterol ) Papaverine chlordiazepoxide 1. Stewart DJ, Inaba T, Lucassen M, Kalow W. Clin. Pharmacol. Ther. April 1979; 25 ed: 464, 264-8. Hydroxyprogesterone Chloralhydrate Benzilic Acid Benzocaine Salicylic Acid Penicillin-G chloroquine 2. Ambre J. J. Anal. Toxicol. 1985; 9:241. Methylphenidate (Ritalin ) Chloramphenicol Benzoic Acid Secobarbital Pentazocine chlorothiazide 3. Hawks RL, CN Chiang. Urine Testing for Drugs of Abuse. National Institute for Drug Abuse (NIDA), Research Methyl Salicylate Chlordiazepoxide Benzoylecgonine Sodium Chloride Pentobarbital chlorpheniramine Monograph 73, 1986. Nabumetone Chlorothiazide Benzphetamine Sodium Formate chlorpromazine 4. Tietz NW. Textbook of Clinical Chemistry. W.B. Saunders Company. 1986; 1735. Nadolol (±) Chlorpheniramine Benzthiazide Sulfamethazine Phencyclidine chlorpropamide 5. FDA Guidance Document: Guidance for Premarket Submission for Kits for Screening Drugs of Abuse to be Used Nafcillin Chlorpromazine Bilirubin Sulfamethoxazole Phenelzine cholesterol by the Consumer, 1997. Nalidixic Acid Chlorquine Bisacodyl Sulfanilamide Phenobarbital clindamycin 6. Robert DeCresce. Drug Testing in the workplace, 114. Nalmefene Cholesterol Bromazepam Sulfathiazole Phentermine clonidine 7. Baselt RC. Disposition of Toxic Drugs and Chemicals in Man. 2nd Ed. Biomedical Publ., Davis, CA 1982; 487. (+/-) Nicotine Clonidine 2-Bromo-a -ergocryptine Sulfisoxazole Trans-2-Phenyl-cylopropylamine-hydrochloride clozapine 8. OSHA, The Bloodborne Pathogens Standard 29, Code of Federal Regulations 29 CFR 1910.1030. Nicotinic Acid Cocaine hydrochloride Brompheniramine Sulindac β-Phenylethlamine colchicine 9. CDC, Centers for Disease Control (CDC) Guidelines, Morbidity and Mortality Weekly Report, Volume 37, Number Nifedipine Codeine Caffeine Talbutal Phenylpropanolamine cortisone 24, 1988. Nitrazepam Cannabidiol Tamoxifen Cortisone Prednisolone creatinine Noscapine Cannabino Tannic Acid (-) Cotinine Prednisone deoxycorticosterone Oxycodone Chloramphenicol Tenoxicam Creatinine Procaine desipramine Oxymetazoline Chlorcyclizine Terbutaline Deoxycorticosterone Promethazine dextromethorphan Oxyphenbutazone Chlordiazepoxide Terfenadine Dextromethorphan D,L-Propanolol diazepam Distributed by: Oxypurinol Chloroquine Tetracycline Diazepam D-Propoxyphene digoxin Paclitaxel Chlorothiazide Tetraethylthiuram Diclofenac D-Pseudoephedrine diphenhydramine Pancuronium Bromide Micro Distributing Chlorotrianisene Tetrahydrozoline Diflunisal Quinidine dipyridamole Papaverine 620 Kennedy Court Chlorpheniramine Theobromine Digoxin Quinine doxycycline Pargyline Belton, TX 76513 Chlorpromazine Theophylline Diphenhydramine Ranitidine erythromycin Penicillin Dimercaprol Thiamine Doxylamine Salicylic acid estradiol 866-933-0964 Pentachlorophenol Dimethylaminoantipyrin Thioridazine Ecgonine hydrochloride Secobarbital estriol www.micro-distributing.com Pentobarbital Dimethyl Isosorbide Tobramycin Ecgonine methylester Serotonin (5-Hydroxytyramine) estrone Pentoxifylline Dimethyl Sulfoxide Tolazamide (IR,2S)-(-)-Ephedrine Sulfamethazine ethanol Pentylenetetrazole Disopyramide Tolbutamide L-Ephedrine Sulindac ethylene glycol p-Phenylenediamine Dobutamine Tolmetin (-) Y Ephedrine Temazepam epinepherine Phenelzine Doxepin Toluene Erythromycin Tetracycline ferrous sulfate Phenformin Doxycycline Trazodone β-Estradiol Tetrahydrocortisone, 3 furosemide Pheniramine Ecgonine Triamcinolone Estrone-3-sulfate Acetate gentamycin Phenobarbital Ecgonine Methyl Ester Triamterene Ethyl-p-aminobenzoate Tetrahydrocortisone 3 (β-D-glucuronide) glucose Phenol Emetine Triazolam Fenoprofen Tetrahydrozoline haloperidol Phenolphthalien Ephedrine Trichlormethiazide Furosemide Thiamine hemoglobin Phenothiazine Epinephrine Trichloroacetic acid Gentisic Thioridazine hydralazine Phenoxymethyl Erythromycin Trifluoperazine Hemoglobin Tolbutamine hydrocortisone Effective date: 3/10/2010 Penicillinic acid ( Penicillin V ) Estriol Triflupromazine Hydralazine Triamterene hydroxycarbalamine Phentolamine Estrone Trimethobenzamide Hydrochlorothiazide Trifluoperazine hydroxyprogesterone Phenylbutazone Ethyl-p-aminobenzoate Trimethoprim Hydrocodone Trimethoprim hydroxyzine Phenylethylamine Etodolac Trimipramine Hydrocortisone D, L-Tryptophan ibuprofen Phenylpropanolamine Etoposide Triprolidine p-Hydroxyamphetamine Tyramine indomethacin Phenyltoloxamine Famotidine Tropic Acid O-Hydroxyhippuric D, L-Tyrosine lidocaine Picrotoxin Fenfluramine Tropine p-Hydroxy-methamphetamine Uric acid lisinopril Pilocarpine Ferrous Sulfate Tryptamine 3-Hydroxytyramine Verapamil lithium Pimozide Flufenamic Acid Tyramine Ibuprofen Zomepirac loperamide Pinacidil Flunisolide Urea ( Carbamide ) Iproniazid lorazepam Pindolol Formaldehyde Uric Acid (-) Isoproterenol Non Cross-Reacting LSD Pipecolic Acid Furosemide Vancomycin Isoxsuprine Compound of metronidazole Pipedemic Acid Gemfibrozil Vincamine Ketamine Methylenedioxymethamp naproxen Piroxicam Gentamicin Sulfate Xylometazoline Ketoprofen niacinamide Potassium Chloride heamine Gentisic Acid Yohimbine Labetalol nicotine Potassium Iodide *Parent compound only： Glucose Zearalenone Levorphanol nifedipine Prazepam Hemoglobin Zomepirac Loperamide acetaldehyde nitrofurantoin Prazosin Hydralazine Zopiclone Meperidine acetaminophen nortriptyline Prednisone Hydrastine Meprobamate acetazolamide ofloxacin Prilocaine Hydrochlorothiazide Non Cross-Reacting Methadone acetone oxalic acid Primaquine Hydrocodone Compound of Tricyclic D-methamphetamine albumin penicillin G Primidone Hydrocortisone Antidepressants Methoxyphenamine albuterol pentobarbital Proadifen Hydrocarbalamine *Parent compound only： ammonium phenobarbital Hydroflumethiazide