This form should be used for all taxonomic proposals. Please complete all those modules that are applicable (and then delete the unwanted sections). For guidance, see the notes written in blue and the separate document “Help with completing a taxonomic proposal”

Please try to keep related proposals within a single document; you can copy the modules to create more than one genus within a new family, for example.

MODULE 1: TITLE, AUTHORS, etc

(to be completed by ICTV Code assigned: 2016.013aM officers) Short title: One (1) new species in the genus Bornavirus (e.g. 6 new species in the genus Zetavirus) Modules attached 1 2 3 4 5 (modules 1 and 9 are required) 6 7 8 9

Author(s) with e-mail address(es) of the proposer: The ICTV Bornaviridae Study Group:

Rubbenstroth, Dennis Chair Germany [email protected]

Briese, Thomas Member USA [email protected]

Duerrwald, Ralf Member Germany [email protected]

Horie, Masayuki Member Japan [email protected]

Kuhn, Jens H. Member USA [email protected]

Nowotny, Norbert Member Austria [email protected]

Payne, Susan Member USA [email protected]

Schwemmle, Martin Member Germany [email protected]

Tomonaga, Keizo Member Japan tomonaga@virus.kyoto-u.ac.jp and: Yiming Bao, [email protected] Martin Beer, [email protected]

Page 1 of 10 List the ICTV study group(s) that have seen this proposal: A list of study groups and contacts is provided at http://www.ictvonline.org/subcommittees.asp . If in doubt, contact the appropriate subcommittee chair (fungal, invertebrate, plant, prokaryote or vertebrate viruses)

ICTV-EC or Study Group comments and response of the proposer:

Date first submitted to ICTV: July 12, 2016 Date of this revision (if different to above):

Page 2 of 10 MODULE 2: NEW SPECIES creating and naming one or more new species. If more than one, they should be a group of related species belonging to the same genus. All new species must be placed in a higher taxon. This is usually a genus although it is also permissible for species to be “unassigned” within a subfamily or family. Wherever possible, provide sequence accession number(s) for one isolate of each new species proposed. Code 2016.013aM (assigned by ICTV officers) To create 1 new species within: Fill in all that apply. Genus: Bornavirus  If the higher taxon has yet to be created (in a later module, below) write Subfamily: “(new)” after its proposed name. Family: Bornaviridae  If no genus is specified, enter Order: Mononegavirales “unassigned” in the genus box. Name of new species: Representative isolate: GenBank sequence accession number(s) Mammalian 2 bornavirus variegated squirrel bornavirus 1 LN713681 (VSBV-1) isolate “squirrel brain”

Reasons to justify the creation and assignment of the new species:  Explain how the proposed species differ(s) from all existing species. o If species demarcation criteria (see module 3) have previously been defined for the genus, explain how the new species meet these criteria. o If criteria for demarcating species need to be defined (because there will now be more than one species in the genus), please state the proposed criteria.  Further material in support of this proposal may be presented in the Appendix, Module 9 As of 2016, the family Bornaviridae includes one genus (Bornavirus). Until early 2015, this genus included a single species (Borna disease virus) for viruses originating from mammalian hosts. However, since 2008 several distinct bornaviruses have been discovered in avian, reptilian, and mammalian hosts. In the past two years, bornavirus taxonomy was adapted to accommodate this increased variability (see newest taxonomy release by the ICTV). The family remains mono-generic, but the genus now includes seven species: Elapid 1 bornavirus, Mammalian 1 bornavirus (formerly Borna disease virus), Passeriform 1 bornavirus, Passeriform 2 bornavirus, Psittaciform 1 bornavirus, Psittaciform 2 bornavirus, and Waterbird 1 bornavirus (Kuhn et al., 2015; Rubbenstroth et al., 2015). The majority of known bornaviruses are classified in these species, but four additional bornaviruses remain unclassified due to insufficient sequence information (Table 2).

Criteria for bornavirus species demarcation are based on genomic characteristics, including PAirwise Sequence Comparison (PASC) (Bao et al., 2012, 2014), in combination with biological characteristics, such as antigenic relationship (Zimmermann et al., 2014) and natural host range (Kuhn et al., 2015). In agreement with these additional criteria, the range of the species differentiation cut-off for PASC of coding-complete genome sequences was defined as 72–75% (Kuhn et al., 2015; Rubbenstroth et al., 2015).

Within the past year, a new mammalian bornavirus, named “variegated squirrel bornavirus 1 (VSBV-1)”, was identified. The virus was discovered in Germany in healthy captive squirrels

Page 3 of 10 of the family Sciuridae, such as variegated squirrels (Sciurus variegatoides) (Hoffmann et al., 2015). VSBV-1 is a zoonotic virus as accidental transmission to humans exposed to infected squirrels resulted in fatal neurologic disease in at least three cases (Hoffmann et al., 2015). VSVB-1 coding-complete genomes were deposited to GenBank (LN713680, LN713681). The highest nucleotide identity of the VSBV-1 genome (67.5%) is shared with the genome of Borna disease virus 1 (species Mammalian 1 bornavirus). This value is below the previously defined bornavirus species differentiation cut-off of 71%, but within the range of sequence identities found between bornaviruses of distinct species (58.1–71.0%). Based on the known host range and genome sequence identity values, we propose to assign VSBV-1 to a new species “Mammalian 2 bornavirus” within the genus Bornavirus.

Page 4 of 10 MODULE 9: APPENDIX: supporting material

additional material in support of this proposal References:

Bao, Y., Chetvernin, V., Tatusova, T., 2012. PAirwise Sequence Comparison (PASC) and its application in the classification of filoviruses. Viruses 4, 1318-1327. Bao, Y., Chetvernin, V., Tatusova, T., 2014. Improvements to pairwise sequence comparison (PASC): a genome-based web tool for virus classification. Arch Virol 159, 3293-3304. Hoffmann, B., Tappe, D., Hoper, D., Herden, C., Boldt, A., Mawrin, C., Niederstrasser, O., Muller, T., Jenckel, M., van der Grinten, E., Lutter, C., Abendroth, B., Teifke, J.P., Cadar, D., Schmidt-Chanasit, J., Ulrich, R.G., Beer, M., 2015. A Variegated Squirrel Bornavirus Associated with Fatal Human Encephalitis. N Engl J Med 373, 154-162. Kuhn, J.H., Durrwald, R., Bao, Y., Briese, T., Carbone, K., Clawson, A.N., deRisi, J.L., Garten, W., Jahrling, P.B., Kolodziejek, J., Rubbenstroth, D., Schwemmle, M., Stenglein, M., Tomonaga, K., Weissenbock, H., Nowotny, N., 2015. Taxonomic reorganization of the family Bornaviridae. Arch. Virol. 160, 621-632. Rubbenstroth, D., Briese, T., Durrwald, R., Horie, M., Kuhn, J.H., Nowotny, N., Payne, S., Schwemmle, M., Tomonaga, K., Banyai, K., Bao, Y., Farkas, S., Marton, S. 2015. Two new species in the family Bornaviridae. Zimmermann, V., Rinder, M., Kaspers, B., Staeheli, P., Rubbenstroth, D., 2014. Impact of antigenic diversity on laboratory diagnosis of Avian bornavirus infections in birds. J. Vet. Diagn. Invest. 26, 769-777.

Page 5 of 10 Annex: Include as much information as necessary to support the proposal, including diagrams comparing the old and new taxonomic orders. The use of Figures and Tables is strongly recommended but direct pasting of content from publications will require permission from the copyright holder together with appropriate acknowledgement as this proposal will be placed on a public web site. For phylogenetic analysis, try to provide a tree where branch length is related to genetic distance.

Table 1. Sequences used for PASC analysis

Page 6 of 10 Figure 1. Histogram of Bornaviridae PASC analysis. Distribution of pairwise identities among complete sequences of 22 viruses in the family Bornaviridae. The histogram is colored as if the taxonomy proposed here was accepted by the ICTV and then adopted by NCBI. Peaks above 72% identity (green) represent genome pairs belonging to the same species. Peaks below 71% identity (yellow) represent genome pairs belonging to different species but the same genus. X-axis, percentage of identity; y-axis, number of genome pairs.

Page 7 of 10 Table 2. Proposed classification and nomenclature of bornaviruses

Family Genus (name Species Virus (virus abbreviation) (name of of taxon taxon members) members)

Bornaviridae (bornavirids, bornaviruses) Bornavirus (bornaviruses) Mammalian 1 bornavirus Borna disease virus 1 (BoDV-1) Borna disease virus 2 (BoDV-2) Mammalian 2 bornavirus variegated squirrel bornavirus 1 (VSBV-1) Psittaciform 1 bornavirus parrot bornavirus 1 (PaBV-1) parrot bornavirus 2 (PaBV-2) parrot bornavirus 3 (PaBV-3) parrot bornavirus 4 (PaBV-4) parrot bornavirus 7 (PaBV-7) Passeriform 1 bornavirus canary bornavirus 1 (CnBV-1) canary bornavirus 2 (CnBV-2) canary bornavirus 3 (CnBV-3) Waterbird 1 bornavirus aquatic bird bornavirus 1 (ABBV-1) aquatic bird bornavirus 2 (ABBV-2) Passeriform 2 bornavirus estrildid finch bornavirus 1 (EsBV-1) Psittaciform 2 bornavirus parrot bornavirus 5 (PaBV-5) Elapid 1 bornavirus Loveridge´s garter snake virus 1 (LGSV-1) tentative, unclassified bornaviruses Gaboon viper virus 1 (GaVV-1) munia bornavirus 1 (MuBV-1) parrot bornavirus 6 (PaBV-6) parrot bornavirus 8 (PaBV-8) 1Type species is underlined. red new virus, previously unclassified

Page 8 of 10 Figure 2. Phylogenetic tree of a 5,571 nt stretch coding for complete N, X, P, M, and G proteins and partial L proteins of 16 bornaviruses (corresponding to pos. 54 to 5,553 of BoDV-1 [U04608]). Phylogenetic neighbor joining analysis was conducted with Geneious R8. The evolutionary distances were computed using the Jukes-Cantor model. Bootstrap resampling analysis with 1,000 replicates was employed; percentages ≥70% are shown next to the branches.

Page 9 of 10 Figure 3. Phylogenetic tree of selected nucleotide sequences (A) or amino acid sequences (B) of the P protein gene of 18 bornaviruses. Phylogenetic neighbor joining analysis was conducted with Geneious R8. The evolutionary distances were computed using the Jukes-Cantor model. Bootstrap resampling analysis with 1,000 replicates was employed; percentages ≥70% are shown next to the branches. (*) Asterisks indicate bornaviruses not classified due to lack of sufficient sequence information.

(A)

(B)

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