RAD9A (Phospho Y28) Polyclonal Antibody

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RAD9A (phospho Y28) polyclonal is found to possess 3' to 5' exonuclease activity, which antibody may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with Catalog Number: PAB0589 RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, Regulatory Status: For research use only (RUO) which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA Product Description: Rabbit polyclonal antibody raised sites has been noted for this gene. [provided by RefSeq] against synthetic phosphopeptide of RAD9A. References: Immunogen: Synthetic phosphopeptide (conjugated 1. Accumulation of hRad9 protein in the nuclei of with KLH) corresponding to residues surrounding Y28 of nonsmall cell lung carcinoma cells. Maniwa Y, human RAD9A. Yoshimura M, Bermudez VP, Yuki T, Okada K, Kanomata N, Ohbayashi C, Hayashi Y, Hurwitz J, Okita Host: Rabbit Y. Cancer. 2005 Jan 1;103(1):126-32. 2. The human Rad9-Rad1-Hus1 checkpoint complex Reactivity: Human stimulates flap endonuclease 1. Wang W, Brandt P, Rossi ML, Lindsey-Boltz L, Podust V, Fanning E, Sancar Applications: WB-Ce A, Bambara RA. Proc Natl Acad Sci U S A. 2004 Nov (See our web site product page for detailed applications 30;101(48):16762-7. Epub 2004 Nov 19. information) 3. The human Rad9 checkpoint protein stimulates the Protocols: See our web site at carbamoyl phosphate synthetase activity of the http://www.abnova.com/support/protocols.asp or product multifunctional protein CAD. Lindsey-Boltz LA, Wauson page for detailed protocols EM, Graves LM, Sancar A. Nucleic Acids Res. 2004 Aug 23;32(15):4524-30. Print 2004. Form: Liquid Purification: Protein G purification Recommend Usage: Western Blot (1:1000) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS (0.09% sodium azide) Storage Instruction: Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 5883 Gene Symbol: RAD9A Gene Alias: RAD9 Gene Summary: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein Page 1/1 Powered by TCPDF (www.tcpdf.org).

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A Computational Approach for Defining a Signature of Β-Cell Golgi Stress in Diabetes Mellitus
Page 1 of 781 Diabetes A Computational Approach for Defining a Signature of β-Cell Golgi Stress in Diabetes Mellitus Robert N. Bone1,6,7, Olufunmilola Oyebamiji2, Sayali Talware2, Sharmila Selvaraj2, Preethi Krishnan3,6, Farooq Syed1,6,7, Huanmei Wu2, Carmella Evans-Molina 1,3,4,5,6,7,8* Departments of 1Pediatrics, 3Medicine, 4Anatomy, Cell Biology & Physiology, 5Biochemistry & Molecular Biology, the 6Center for Diabetes & Metabolic Diseases, and the 7Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202; 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN, 46202; 8Roudebush VA Medical Center, Indianapolis, IN 46202. *Corresponding Author(s): Carmella Evans-Molina, MD, PhD ([email protected]) Indiana University School of Medicine, 635 Barnhill Drive, MS 2031A, Indianapolis, IN 46202, Telephone: (317) 274-4145, Fax (317) 274-4107 Running Title: Golgi Stress Response in Diabetes Word Count: 4358 Number of Figures: 6 Keywords: Golgi apparatus stress, Islets, β cell, Type 1 diabetes, Type 2 diabetes 1 Diabetes Publish Ahead of Print, published online August 20, 2020 Diabetes Page 2 of 781 ABSTRACT The Golgi apparatus (GA) is an important site of insulin processing and granule maturation, but whether GA organelle dysfunction and GA stress are present in the diabetic β-cell has not been tested. We utilized an informatics-based approach to develop a transcriptional signature of β-cell GA stress using existing RNA sequencing and microarray datasets generated using human islets from donors with diabetes and islets where type 1(T1D) and type 2 diabetes (T2D) had been modeled ex vivo. To narrow our results to GA-specific genes, we applied a filter set of 1,030 genes accepted as GA associated.
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Complex Interacts with WRN and Is Crucial to Regulate Its Response to Replication Fork Stalling
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