Dual Antidepressant Duloxetine Blocks Nicotinic Receptor Currents, Calcium Signals and Exocytosis in Chromaffin Cells Stimulated with Acetylcholine S
Supplemental material to this article can be found at: http://jpet.aspetjournals.org/content/suppl/2018/07/13/jpet.118.250969.DC1 1521-0103/367/1/28–39$35.00 https://doi.org/10.1124/jpet.118.250969 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS J Pharmacol Exp Ther 367:28–39, October 2018 Copyright ª 2018 by The American Society for Pharmacology and Experimental Therapeutics Dual Antidepressant Duloxetine Blocks Nicotinic Receptor Currents, Calcium Signals and Exocytosis in Chromaffin Cells Stimulated with Acetylcholine s Carmen Nanclares, Isabel Gameiro-Ros, Iago Méndez-López, Carmen Martínez-Ramírez, J. Fernando Padín-Nogueira, Inés Colmena, Andrés M. Baraibar, Luis Gandía,1 and Antonio G. García1 Instituto Teófilo Hernando and Departamento de Farmacología, Facultad de Medicina (C.N., I.G.-R., I.M.-L., C.M.-R., J.F.P.-N., I.C., A.M.B., L.G., A.G.G.) and Instituto de Investigación Sanitaria, Hospital Universitario de La Princesa (A.G.G.), Universidad Downloaded from Autónoma de Madrid, Madrid, Spain; and Departamento de Ciencias Médicas, Facultad de Medicina, Universidad Castilla La Mancha (UCLM), Ciudad Real, Spain (J.F.P.-N.) Received May 28, 2018; accepted July 12, 2018 ABSTRACT jpet.aspetjournals.org The inhibition of nicotinic acetylcholine receptors (nAChRs) partially use dependent. The ACh-elicited membrane depolar- 21 has been proposed as a potential strategy to develop new antide- ization, the elevation of cytosolic calcium ([Ca ]c), and cate- pressant drugs. This is based on the observation that antide- cholamine release in BCCs were also blocked by duloxetine. This pressants that selectively block noradrenaline (NA) or serotonin blockade developed slowly, and the recovery of secretion was (5-HT) reuptake also inhibit nAChRs.
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