bioRxiv preprint doi: https://doi.org/10.1101/391516; this version posted May 2, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Transcriptomic response in symptomless roots of clubroot infected 2 kohlrabi (Brassica oleracea var. gongylodes) mirrors resistant plants 3 4 Stefan Ciaghi1,$, Arne Schwelm1,2,$, Sigrid Neuhauser1,* 5 6 1 University of Innsbruck, Institute of Microbiology, Technikerstraße 25, 6020 7 Innsbruck, Austria 8 2 Swedish University of Agricultural Sciences, Department of Plant Biology, Uppsala 9 BioCenter, Linnean Centre for Plant Biology, P.O. Box 7080, SE-75007 Uppsala, 10 Sweden 11 12 * Correspondence:
[email protected] 13 $ Contributed equally to this work 14 15 Abstract 16 Background: Clubroot disease caused by Plasmodiophora brassicae (Phytomyxea, 17 Rhizaria) is one of the economically most important diseases of Brassica crops. The 18 formation of hypertrophied roots accompanied by altered metabolism and hormone 19 homeostasis is typical for infected plants. Not all roots of infected plants show the same 20 phenotypic changes. While some roots remain uninfected, others develop galls of 21 diverse size. The aim of this study was to analyse and compare the intra-plant 22 heterogeneity of P. brassicae root galls and symptomless roots of the same host plants 23 (Brassica oleracea var. gongylodes) collected from a commercial field in Austria using 24 transcriptome analyses. 25 Results: Transcriptomes were markedly different between symptomless roots and gall 26 tissue.