SAN TA C RUZ BI OTEC HNOL OG Y, INC . OR2AJ1 (P-13): sc-104521

BACKGROUND APPLICATIONS Olfactory receptors are G -coupled receptors that localize to the cilia OR2AJ1 (P-13) is recommended for detection of OR2AJ1 of human origin of olfactory sensory neurons where they display affinity for and bind to a by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000), variety of odor molecules. The encoding olfactory receptors comprise immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and the largest family in the . The binding of solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000); may to odor molecules triggers a signal transduction that propagates cross-react with OR2T27. nerve impulses throughout the body, ultimately leading to transmission of the OR2AJ1 (P-13) is also recommended for detection of OR2AJ1 in additional signal to the brain and the subsequent perception of smell. OR2AJ1 (olfac - species, including equine, canine, bovine and porcine. tory receptor 2AJ1) is a 328 amino acid protein. The encoding OR2AJ1 maps to human 1. RECOMMENDED SECONDARY REAGENTS REFERENCES To ensure optimal results, the following support (secondary) reagents are recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 1. Malnic, B., Hirono, J., Sato, T. and Buck, L.B. 1999. Combinatorial receptor (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey codes for odors. Cell 96: 713-723. anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ 2. Glusman, G., Bahar, A., Sharon, D., Pilpel, Y., White, J. and Lancet, D. Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: 2000. The olfactory receptor gene superfamily: data mining, classification, sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- and nomenclature. Mamm. Genome 11: 1016-1023. rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) 3. Gaillard, I., Rouquier, S. and Giorgi, D. 2004. Olfactory receptors. Cell. with UltraCruz™ Mounting Medium: sc-24941. Mol. Life Sci. 61: 456-469. 4. Buck, L.B. 2004. Olfactory receptors and odor coding in mammals. Nutr. RESEARCH USE Rev. 62: S184-S188; discussion S224-S241. For research use only, not for use in diagnostic procedures. 5. Malnic, B., Godfrey, P.A. and Buck, L.B. 2004. The human olfactory receptor gene family. Proc. Natl. Acad. Sci. USA 101: 2584-2589. 6. Khafizov, K., Anselmi, C., Menini, A. and Carloni, P. 2007. Ligand specificity of odorant receptors. J. Mol. Model. 13: 401-409. 7. Rinaldi, A. 2007. The scent of life. The exquisite complexity of the sense of smell in animals and humans. EMBO Rep. 8: 629-633.

CHROMOSOMAL LOCATION Genetic locus: OR2AJ1 (human) mapping to 1p36.33.

SOURCE OR2AJ1 (P-13) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an extracellular domain of OR2AJ1 of human origin.

PRODUCT Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. Blocking peptide available for competition studies, sc-104521 P, (100 µg peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA).

STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.

PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and support products.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com