CURRICULUM VITAE

Robert Joseph Beattie

Personal Information

Email: [email protected] Phone: +43 664 8832 6139 Address: IST Austria, Lab Building East, Am Campus 1, Klosterneuburg 3400, Austria Personal Website: http://beattiescience.com Educational Website: http://scopeseducation.org ORCID: 0000-0002-8483-8753

Education

2014 Ph.D., Biomedical Science, The University of Sheffield, Sheffield, UK 2009 B.Sc., Honors (Co-op), Microbiology, The University of Manitoba, Winnipeg, Canada

Academic Experience

Main Areas of Research Neurobiology, Neurogenetics, Gliogenesis, Developmental Biology

2015 – Present Postdoctoral fellow in the lab of Dr. Simon Hippenmeyer at IST Austria, Klosterneuburg, Austria, Laboratory of Genetic Dissection of Cerebral Cortex Development. Since 03.2017, supported by FWF Lise Meitner Fellowship.

2014 - 2015 (11 months) Postdoctoral researcher in the lab of Dr. Verdon Taylor at the University of Basel, Switzerland, Laboratory of Adult Neurogenesis and Cortical Development.

2009 – 2014 Ph.D. student in the lab of Dr. Verdon Taylor in the Department of Biomedical Science at The University of Sheffield, UK and Department of Biomedicine, University of Basel, Switzerland. Laboratory of Adult Neurogenesis and Cortical Development. Ph.D. thesis: The Role of Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation in the Neurogenic Niche.

2008 - 2009 (12 months) Honours student in the lab of Dr. Steve Whyard in the department of Biological Sciences at the University of Manitoba, Canada. Laboratory of Molecular Basis of Development.

2008 (4 months) Undergraduate internship in the lab of Dr. Verdon Taylor at the Max-Planck Institute of Immunobiology and Epigenetics, Germany, Laboratory of Adult Neurogenesis.

2007 - 2008 (16 months) Undergraduate internship in the lab of Dr. Ute Stroeher in the Special Pathogens Unit at the National Microbiology Lab Public Health Agency of Canada, Canada, Laboratory of Ebola Vaccine Development.

Grants and Fellowships

2017 FWF Lise Meitner Fellowship - Value: 160, 000 EUR (~$250, 000 CAD) 2012 Swiss Society for Neuroscience travel grant 2010 Career Development and Transferable Skills Training Grant 2008 Millennium Student Success Grant 2004 Undergraduate Student Scholarship

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Robert J. Beattie, Ph.D.

Selected Honors, Academic Prizes and Awards

2019 Best Poster Award - Development and Stem Cells Meeting - Vienna, Austria 2017 Best Poster Award - AXON Meeting - Vienna, Austria 2013 Best Poster Award - University of Basel Department of Biomedicine - Basel, Switzerland 2010 International Student Ambassador Award - Max Planck Institute of Immunobiology and Epigenetics 2008 Honorary mention Canadian Co-op Student of the year award 2008 Manitoba Co-op Student of the year award 2004 Grade 12 Valedictorian

Teaching Activities & Extracurricular

2020 Undergraduate Mentoring Program, University of Manitoba, Canada Mentor - Providing career advice for those considering internships and postgraduate studies.

2020 Introduction to Advanced Methods in Neuroscience, Postgraduate level course, IST Austria - 3 credit hours Course developer and lecturer (Fall semester) – I designed this course to introduce students to advanced techniques used in neuroscience. It highlighted the current strengths and limitations of each method, what questions it can be used to address, and the requirements for planning and running such experiments.

2020 Neuroscience Seminar Series Organizing Committee, IST Austria Seminar series organizer – Responsible for inviting and hosting Dr. Beth Stevens (Harvard University); Dr. Oscar Marin (King's College London)

2019 Undergraduate Mentoring Program, University of Manitoba, Canada Mentor - Providing career advice for those considering internships and postgraduate studies.

2019 Neuroscience Seminar Series Organizing Committee, IST Austria Seminar series organizer – Responsible for inviting and hosting Dr. Bosiljka Tasic (Allen Institute), Dr. Rafael Yuste (Columbia University)

2018 Undergraduate Mentoring Program, University of Manitoba, Canada Mentor - Providing career advice for those considering internships and postgraduate studies.

2018 Developmental Neurobiology and Brain Diseases, Postgraduate level course, IST Austria - 3 credit hours Lecturer - In addition to lecturing, this course used a series of research articles to stimulate discussion.

2016 Principles of Neuronal Circuit Assembly, Postgraduate level course, IST Austria - 3 credit hours Teaching Assistant - I led the laboratory portion of the course, which involved one-on-one training of basic neuroscience techniques and protocols in tissue preparation for confocal microscopy.

2016 Animal Handling and Manipulation Course, Postgraduate level course, IST Austria - 3 credit hours Instructor – Introductory course for scientists who handle animals at IST Austria. I taught advanced gene delivery methods such as in utero electroporation and helped students perform mouse surgeries.

2012 Biomedical Sciences Ph.D. Society, The University of Sheffield, Sheffield, UK Ph.D. Representative – Acted as a liaison between students and faculty. Designed workshops and lecture series, invited guest speakers, organized charity fundraisers and compiled final reports for funding bodies. A project I am proud of spearheading was the inaugural Ph.D. retreat, which continues annually to this day.

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Robert J. Beattie, Ph.D.

Supervision of Laboratory Trainees and Graduate Students

I have mentored and supervised nine junior trainees with different stages of experience. Mentoring involved teaching relevant subject matter, experimental techniques and directly supervising projects related to my research.

2020 Oct – Present Ishita Gupta Ph.D. rotation student in the Hippenmeyer lab 2020 Aug - Present Ines Aykara Undergraduate internship student in the Hippenmeyer lab 2020 (Winter) Florian Schmidt Ph.D. rotation student in the Hippenmeyer lab 2018 (Winter) Kasumi Kishi Ph.D. rotation student in the Hippenmeyer lab 2018 (Fall) Lena Schwarz Ph.D. rotation student in the Hippenmeyer lab 2018 (Summer) Jonáš Rybníček Undergraduate internship student in the Hippenmeyer lab 2017 (Fall) Julia Michalska Ph.D. rotation student in the Hippenmeyer lab 2016 (Summer) Laura Burnett Undergraduate internship student in the Hippenmeyer lab 2011-2013 Tanzila Muhktar Master's student in the Taylor lab

Pedagogy and Leadership Courses

2019 (Fall) Teaching Didactics Workshop IST Austria, Klosterneuburg, Austria 2017 (Summer) Science Education Workshop IST Austria, Klosterneuburg, Austria 2017 (Spring) Project Management Workshop IST Austria, Klosterneuburg, Austria 2013 (Winter) Biotechnology Startup Course University of Basel, Basel, Switzerland 2011 (Fall) Biotechnology YES The University of Sheffield, Sheffield, England

Science Outreach Activities

2021 Maker Faire Vienna Vienna, Austria Speaker 2020 Elementary School Visit Winnipeg, Canada Presenter 2020 Pop-up Science IST Austria Vienna, Austria Presenter 2020 MADM Brain Explorer Vienna, Austria Creator (Web App) 2019 Long Night of Museums Vienna, Austria Presenter 2019 Scopes Education Vienna, Austria Co-founder 2019 Maker Faire Vienna Vienna, Austria Presenter 2018 IST Austria Open Campus Klosterneuburg, Austria Instructor 2018 Long Night of Research Klosterneuburg, Austria Instructor 2017 IST Austria Summer Camp Klosterneuburg, Austria Instructor 2017 IST Austria Open Campus Klosterneuburg, Austria Instructor 2011 Elementary School Visit Sheffield, England Instructor

Invited Talks

2021 Maker Faire Vienna – Vienna Austria (Rescheduled from 2020 due to COVID-19) SCOPES: Sparking Curiosity Through Open-Source Platforms in Education and Science.

2020 Glial Cells-Neuron Crosstalk in CNS Health and Disease - Turin, Italy Molecular Mechanisms Regulating Gliogenesis in the Neocortex. Chairperson for Glia-neuron crosstalk in CNS functions and memory session.

2020 IST Austria Life Science Retreat - Retz, Austria Imprinted Cdkn1c Genomic Locus Cell-Autonomously Promotes Cell Survival in Cerebral Cortex Development.

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Robert J. Beattie, Ph.D.

2019 ANA Meeting 2019 - Innsbruck, Austria Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development.

2018 Development and Stem Cells Meeting 2018 - Vienna, Austria Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development.

2017 IST Austria Life Science Retreat - Sopron, Hungary Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.

2017 Cortical Development 2017 - Crete, Greece Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.

2016 Vienna Neuroscience Network Meeting - Vienna, Austria Lgl1 Controls NSC Lineage Progression via Intrinsic and Cell-non-autonomous Mechanisms.

2014 The 8th Notch Meeting - Athens, Greece Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation Towards an Oligodendrocytic Lineage in Cells Originating from the Neurogenic Forebrain Niche.

2014 University of Fribourg - Fribourg, Switzerland Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation Towards an Oligodendrocytic Lineage in Cells Originating from the Neurogenic Forebrain Niche.

Professional Training

2020 Successful Interview Technique Workshop by Sarah Blackford. IST Austria – Klosterneurburg, Austria 2020 Resilience & Well-Being in Academia Workshop. IST Austria - Klosterneuburg, Austria 2020 Research Integrity and Research Ethics Workshop. IST Austria - Klosterneuburg, Austria 2013 RNA-sequencing: From experiment planning to statistical data analysis. Department of Biosystems Science and Engineering, ETH Zürich - Basel, Switzerland. 2013 R Course for Bioinformatics. Friedrich Miescher Institute for Biomedical Research (FMI) - Basel, CH 2013 LTK mouse experimentation course. Federation for Laboratory Animal Science Associations (FELASA) certification - Basel, Switzerland 2011 Home Office Animal License Course. The University of Sheffield - Sheffield, England 2010 EMBO Animal Models for Physiology and Disease. The University of Sheffield - Sheffield, England 2010 Molecular and Cellular Biology Practical Course. International Max Planck Research School - MPI for Immunobiology and Epigenetics, Freiburg, Germany

Memberships in Scientific Societies

2019 – Current Austrian Society for Neuroscience (ANA) 2019 – Current Society for Neuroscience (SfN) 2019 – 2020 Austrian Association of Molecular Life Sciences and Biotechnology (ÖGMBT) 2012 – 2015 Swiss Society of Neuroscience (SSN) 2013 – 2015 International Society for Stem Cell Research (ISSCR) 2012 – 2013 British Neuroscience Association (BNA)

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Robert J. Beattie, Ph.D.

PUBLICATION LIST

Google Scholar: https://scholar.google.at/citations?user=c3UxJdkAAAAJ&hl=en

Forthcoming Publications

Beattie, R., Streicher, C., Pauler, F., Laukoter, S., Farlik, M., Krausgruber, T., Bock, C., Hippenmeyer, S. "Cell-autonomous molecular mechanisms regulating embryonic and postnatal cortical development." (Manuscript in preparation)

Beattie R, Stouffer M, Cheung G, Hippenmeyer S. "Molecular Mechanisms Regulating Glia Lineages in Health and Disease." Invited review for Frontiers in Cellular Neurobiology (Manuscript in preparation)

Pauler, F.M.*, Beattie, R.*, Laukoter, S.*, Streicher, C., Farlik, M., Krausgruber, T., Bock, C., Hippenmeyer, S. "Cell-autonomous and community effects of single gene deletion on the temporal progression of neocortex development revealed by MADM and RNA-sequencing." (Manuscript in preparation) (*equal contribution)

Publications From Postdoc

Zhang, X.*, Mennicke, C.*, Xiao, G.*, Beattie, R., Haider, M., Hippenmeyer, S., Ghashghaei, HT. "Clonal analysis of gliogenesis in the cerebral cortex reveals stochastic expansion of glia versus neurons and cell autonomous responses to Egfr dosage." Cells (*equal contribution)

Laukoter S.*, Pauler, F.M.*, Beattie, R., Amberg, N., Hansen, A.H., Streicher, C., Penz, T., Bock, C., Hippenmeyer, S. "Cell Type Specificity of Genomic Imprinting in Cerebral Cortex" Neuron, 2020. 107(6): 1160-1179.E9. (*equal contribution) Highlighted in a preview: DeVeale et al., 2020 Neuron 107(6):994-996

Beattie, R.*#, Hippenmeyer, S.*, Pauler F.M.*#. "SCOPES: Sparking Curiosity Through Open-Source Platforms in Education and Science." Frontiers in Education, 2020 (*corresponding author, #equal contribution)

Beattie, R., Streicher, C., Amberg, N., Cheung G., Contreras, X., Hansen, A.H., Hippenmeyer, S., "Lineage Tracing and Clonal Analysis in the Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)" JoVE, 2020

Amberg N*, Beattie R*. "To recombine or not to recombine" eNeuro Blog Reader's Pick, 2020 (*equal contribution)

Laukoter, S.*, Beattie, R.*, Pauler, F.M.*, Amberg, N., Nakayama, K.I., Hippenmeyer, S. "Imprinted Cdkn1c genomic locus cell-autonomously promotes cell survival in cerebral cortex development." Nature Communications, 2020 (*equal contribution)

Llorca, A., Ciceri, G., Beattie, R., Wong, F.K., Diana, D., Serafeimidou, E., Fernández-Otero, M., Streicher, C., Arnold, S.J., Meyer, M., Hippenmeyer, S., Maravall, M., Marin, O., "A stochastic framework of neurogenesis underlies the assembly of neocortical cytoarchitecture." eLife, 2019

Beattie, R., M.P. Postiglione, L. Burnett, S. Laukoter, C. Streicher, F. Pauler, G. Xiao, O. Klezovitch, V. Vasioukhin, T. Ghashgaei, S. Hippenmeyer. "Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells." Neuron , 2017. 94(3): 517-533. Highlighted in two previews: Akhtar et al., 2017 Neuron 94(3):417-420; and Sokol, 2017 Dev Cell 41(5):453-454.

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Robert J. Beattie, Ph.D.

Beattie, R. and S. Hippenmeyer, "Mechanisms of Radial Glia Progenitor Lineage Progression." FEBS Letters, 2017 (Peer Reviewed)

Publications From Ph.D.

Beattie, R., C. Rolando, E. Boda, R. Ivanek, A. Buffo, V. Taylor, "Jagged1 regulates a fate switch of neural stem cells to oligodendrocyte precursor cells in vivo." Finalizing manuscript

Pfurr, S., Y. Chu, C. Bohrer, R. Beattie, F. Greulich, K. Mammadzada, M. Hils, S.J. Arnold, N.H. Uhlenhaut, V. Taylor, K. Schachtrup, C. Schachtrup, “The E2A splice variant E47 regulates the differentiation of projection neurons via p57KIP2 during cortical development.” Development, 2017

Rolando, C.*, C. Erni*, A. Grison, R. Beattie, A. Engler, P.J. Gokhale, M. Milo, T. Wegleiter, S. Jessberger, V. Taylor. "Multipotency of Adult Hippocampal NSCs In Vivo Is Restricted by Drosha/NFIB." Cell Stem Cell, 2016. 19(5): 653-662. (*equal contribution)

Rago, L., R. Beattie, V. Taylor and J. Winter (2014). "miR379-410 cluster miRNAs regulate neurogenesis and neuronal migration by fine-tuning N-cadherin." EMBO J 33(8): 906-920.

Beattie, R. (Nov 2014) "The Role of Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation in the Neurogenic Niche." Thesis - The University of Sheffield.

Beattie, R.*, T. Mukhtar*, V. Taylor (2014). "Fundamentals of Neurogenesis and Neural Stem Cell Development." Textbook - Neural Surface Antigens: From Basic Biology Towards Biomedical Applications. Ed. J. Pruszak. Elsevier Science & Technology. (Peer-reviewed) (*equal contribution)

Scientific Communications (Posters)

2019 49th Society for Neuroscience Meeting - Chicago, USA Beattie, R.*, Laukoter, S.*, Pauler, F.M.*, Amberg, N., Nakayama, K.I., Hippenmeyer, S. Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development. Abstr. 276.05. (*equal contribution)

AXON Meeting 2019 – Alicante, Spain Beattie, R.*, Laukoter, S.*, Pauler, F.M.*, Amberg, N., Nakayama, K.I., Hippenmeyer, S. Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development. Abstr. 97. (*equal contribution)

SY-Stem Meeting 2019 - Vienna, Austria Beattie, R.*, Laukoter, S.*, Pauler, F.M.*, Nakayama, K.I., Hippenmeyer, S Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development.

Development and Stem Cells Meeting 2019 – Vienna, Austria Beattie, R.*, Laukoter, S.*, Pauler, F.M.*, Nakayama, K.I., Hippenmeyer, S. Unexpected Role of Imprinted Cdkn1c Genomic Locus in Cortical Development. Abstr. 4. (*equal contribution)

2018 Gordon Research Conference - Neural Development – Newport, Rhode Island Laukoter, S.*, Pauler, F.*, Beattie, R., Amberg, N., Hansen, A.H., Streicher, C., Penz, T., Bock, C., Hippenmeyer, S. Cell-Type Specificity of Genomic Imprinting in Cerebral Cortex. (*equal contribution)

Cold Spring Harbor Laboratory Epigenetics & Chromatin – Cold Spring Harbor, New York Laukoter, S.*, Pauler, F.*, Beattie, R., Amberg, N., Hansen, A.H., Streicher, C., Penz, T., Bock, C., Hippenmeyer, S. Cell-Type Specificity of Genomic Imprinting in Cerebral Cortex. Abstr. 162.

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Robert J. Beattie, Ph.D.

48th Society for Neuroscience Meeting – San Diego, California Llorca, A., Ciceri, G., Beattie, R., Streicher, C., Arnold, S., Maravall, M., Hippenmeyer, S. & Marin, O. Heterogeneous progenitor behaviour orchestrates mammalian cortical development. Abstr. 195.30/A30.

SY-Stem Meeting 2019 - Vienna, Austria Beattie, R., Postiglione, M.P., Burnett, L., Laukoter, S., Streicher, C., Pauler, F., Xiao, G., Klezovitch, O., Vasioukhin, V., Ghashgaei, T., Hippenmeyer, S. Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.

2017 AXON Meeting 2017 - Vienna, Austria Beattie, R., Postiglione, M.P., Burnett, L., Laukoter, S., Streicher, C., Pauler, F., Xiao, G., Klezovitch, O., Vasioukhin, V., Ghashgaei, T., Hippenmeyer, S. Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells. Abstr. P6.

MRC Symposium - The Developing Brain in Health and Disease - London, England Llorca, A., Ciceri, G., Beattie, R., Streicher, C., Arnold, S., Maravall, M., Hippenmeyer, S. & Marin, O. Clonal Analysis of the Development of Pyramidal Cell Lineages in the Mouse Cerebral Cortex.

MRC Symposium - The Developing Brain in Health and Disease - London, England Beattie, R., Postiglione, M.P., Burnett, L., Laukoter, S., Streicher, C., Pauler, F., Xiao, G., Klezovitch, O., Vasioukhin, V., Ghashgaei, T., Hippenmeyer, S. Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells.

2016 47th Society for Neuroscience Meeting Llorca, A., Ciceri, G., Beattie, R., Streicher, C., Arnold, S., Maravall, M., Hippenmeyer, S. & Marin, O. Clonal Analysis of the Development of Pyramidal Cell Lineages in the Mouse Cerebral Cortex. Abstr. 46.

7th Annual Conference on Stem Cell and Regenerative Medicine – Manchester, England Taylor, V., Rolando, V., Erni, A., Beattie, R., Engler, A., Grison, A., Gokhale, P.J., Milo, M., Wegleiter, T., Jessberger, S. Regulation of hippocampal neural stem cell fate.

Gordon Research Conference - Molecular and Cellular Neurobiology - Hong Kong, China Beattie, R., Postiglione, M.P., Burnett, L., Laukoter, S., Streicher, C., Pauler, F., Xiao, G., Klezovitch, O., Vasioukhin, V., Ghashgaei, T., Hippenmeyer, S. Mosaic Analysis with Double Markers Reveals Distinct Sequential Functions of Lgl1 in Neural Stem Cells. Abstr. 2.

2015 The 12th ISSCR Meeting - Stockholm, Sweden Beattie, R., Boda, E., Rolando, C., Buffo, A., Taylor, V. Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation in the Forebrain Neurogenic Niche.

2014 Stem cells in development and disease - Basel, Switzerland Beattie, R., Boda, E., Rolando, C., Buffo, A., Taylor, V. Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation Towards an Oligodendrocytic Lineage in Cells Originating from the Neurogenic Forebrain Niche.

The 9th FENS Forum of Neuroscience - Milan, Italy Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCα Regulate Jagged1 Activation to Control Neurogenesis.

2013 The 11th ISSCR Meeting - Boston, USA Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCα Regulate Jagged1 Activation to Control Neurogenesis.

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Robert J. Beattie, Ph.D.

Annual Meeting of the Basel Stem Cell Network – Basel, Switzerland Beattie, R., Boda, E., Rolando, C., Buffo, A., Taylor, V. Jagged1 as a Pivotal Regulator of Neural Stem Cell Differentiation Towards an Oligodendrocytic Lineage in Cells Originating from the Neurogenic Forebrain Niche.

"From Bench to Bedside" Neuroscience Network Symposium – Basel, Switzerland Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCa Regulate Jagged1 Activation to Control Neurogenesis.

2012 The 8th FENS Forum of Neuroscience - Barcelona, Spain Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCα Regulate Jagged1 to TBLR1 and Histones to Regulate Neurogenesis.

Regulation of Adult Neurogenesis Meeting – Barcelona, Spain Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCα Regulate Jagged1 to TBLR1 and Histones to Regulate Neurogenesis.

2011 Cambridge Neural Stem Cell Symposium - Cambridge, England Beattie, R., Grabosch, A., Rukavina, M., Taylor, V. PICK1 and PKCα Regulate Jagged1 to TBLR1 and Histones to Regulate Neurogenesis.

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