Bee Viruses: Routes of Infection in Hymenoptera
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Evidence for and Against Deformed Wing Virus Spillover from Honey Bees to Bumble Bees: a Reverse Genetic Analysis Olesya N
www.nature.com/scientificreports OPEN Evidence for and against deformed wing virus spillover from honey bees to bumble bees: a reverse genetic analysis Olesya N. Gusachenko1*, Luke Woodford1, Katharin Balbirnie‑Cumming1, Eugene V. Ryabov2 & David J. Evans1* Deformed wing virus (DWV) is a persistent pathogen of European honey bees and the major contributor to overwintering colony losses. The prevalence of DWV in honey bees has led to signifcant concerns about spillover of the virus to other pollinating species. Bumble bees are both a major group of wild and commercially‑reared pollinators. Several studies have reported pathogen spillover of DWV from honey bees to bumble bees, but evidence of a sustained viral infection characterized by virus replication and accumulation has yet to be demonstrated. Here we investigate the infectivity and transmission of DWV in bumble bees using the buf-tailed bumble bee Bombus terrestris as a model. We apply a reverse genetics approach combined with controlled laboratory conditions to detect and monitor DWV infection. A novel reverse genetics system for three representative DWV variants, including the two master variants of DWV—type A and B—was used. Our results directly confrm DWV replication in bumble bees but also demonstrate striking resistance to infection by certain transmission routes. Bumble bees may support DWV replication but it is not clear how infection could occur under natural environmental conditions. Deformed wing virus (DWV) is a widely established pathogen of the European honey bee, Apis mellifera. In synergistic action with its vector—the parasitic mite Varroa destructor—it has had a devastating impact on the health of honey bee colonies globally1,2. -
An Immunomarking Method to Determine the Foraging Patterns Of
An immunomarking method to determine the foraging patterns of Osmia cornifrons and resulting fruit set in a cherry orchard David Biddinger, Neelendra Joshi, Edwin Rajotte, Noemi Halbrendt, Cassandra Pulig, Kusum Naithani, Mace Vaughan To cite this version: David Biddinger, Neelendra Joshi, Edwin Rajotte, Noemi Halbrendt, Cassandra Pulig, et al.. An immunomarking method to determine the foraging patterns of Osmia cornifrons and resulting fruit set in a cherry orchard. Apidologie, Springer Verlag, 2013, 44 (6), pp.738-749. 10.1007/s13592-013- 0221-x. hal-01201342 HAL Id: hal-01201342 https://hal.archives-ouvertes.fr/hal-01201342 Submitted on 17 Sep 2015 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Apidologie (2013) 44:738–749 Original article * INRA, DIB and Springer-Verlag France, 2013 DOI: 10.1007/s13592-013-0221-x An immunomarking method to determine the foraging patterns of Osmia cornifrons and resulting fruit set in a cherry orchard 1,2 1,2 2 David J. BIDDINGER , Neelendra K. JOSHI , Edwin G. RAJOTTE , 3 1 4 5 Noemi O. HALBRENDT , Cassandra -
Bee Viruses: Routes of Infection in Hymenoptera
fmicb-11-00943 May 27, 2020 Time: 14:39 # 1 REVIEW published: 28 May 2020 doi: 10.3389/fmicb.2020.00943 Bee Viruses: Routes of Infection in Hymenoptera Orlando Yañez1,2*, Niels Piot3, Anne Dalmon4, Joachim R. de Miranda5, Panuwan Chantawannakul6,7, Delphine Panziera8,9, Esmaeil Amiri10,11, Guy Smagghe3, Declan Schroeder12,13 and Nor Chejanovsky14* 1 Institute of Bee Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland, 2 Agroscope, Swiss Bee Research Centre, Bern, Switzerland, 3 Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium, 4 INRAE, Unité de Recherche Abeilles et Environnement, Avignon, France, 5 Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden, 6 Environmental Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand, 7 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand, 8 General Zoology, Institute for Biology, Martin-Luther-University of Halle-Wittenberg, Halle (Saale), Germany, 9 Halle-Jena-Leipzig, German Centre for Integrative Biodiversity Research (iDiv), Leipzig, Germany, 10 Department of Biology, University of North Carolina at Greensboro, Greensboro, NC, United States, 11 Department Edited by: of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States, 12 Department of Veterinary Akio Adachi, Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States, -
Reproduction of the Red Mason Solitary Bee Osmia Rufa (Syn
Eur. J. Entomol. 112(1): 100–105, 2015 doi: 10.14411/eje.2015.005 ISSN 1210-5759 (print), 1802-8829 (online) Reproduction of the red mason solitary bee Osmia rufa (syn. Osmia bicornis) (Hymenoptera: Megachilidae) in various habitats MONIKA FLISZKIEWICZ, ANNA KuśnierczaK and Bożena Szymaś Department of apidology, institute of zoology, Poznań university of Life Sciences, Wojska Polskiego 71c, 60-625 Poznań, Poland; e-mails: [email protected]; [email protected]; [email protected] Key words. Hymenoptera, Megachilidae, Osmia rufa (Osmia bicornis), ecosystem, reproduction, pollination, parasitism Abstract. Osmia rufa L. (Osmia bicornis L.) is a species of a solitary bee, which pollinates many wild and cultivated plants. A total of 900 cocoons containing mature individuals of Osmia rufa L. (450 females and 450 males of a known weight), were placed in each of four habitats (orchard, mixed forest, hay meadow and arboretum of the Dendrology Institute of the Polish Academy of Sciences at Kórnik). These bees were provided with artificial nests made of the stems of common reed. The following parameters were calculated: reproduction dynamics, total number of chambers built by females, mean number of breeding chambers per reed tube and mean num- ber of cocoons per tube. included in the analysis were also the nectar flowers and weather conditions recorded in each of the habitats studied. General linear mixed models indicated that the highest number of chambers was recorded in the hay meadow (6.6 per tube). However, the number of cocoons per tube was similar in the hay meadow, forest and orchard (4.5–4.8 per tube) but was significantly lower in the arboretum (3.0 cocoons per tube on average). -
(Osmia Bicornis, Hymenoptera: Megachilidae) in an Urban
Landscape and Urban Planning 103 (2011) 15–23 Contents lists available at ScienceDirect Landscape and Urban Planning jou rnal homepage: www.elsevier.com/locate/landurbplan Microsite conditions dominate habitat selection of the red mason bee (Osmia bicornis, Hymenoptera: Megachilidae) in an urban environment: A case study from Leipzig, Germany a,b, a,c a,d a Jeroen Everaars ∗, Michael W. Strohbach , Bernd Gruber , Carsten F. Dormann a Helmholtz Centre for Environmental Research – UFZ, Department of Computational Landscape Ecology, Permoserstraße 15, 04318 Leipzig, Germany b Helmholtz Centre for Environmental Research – UFZ, Department of Ecological Modelling, Permoserstraße 15, 04318 Leipzig, Germany c University of Massachusetts Amherst, Department of Environmental Conservation, Holdsworth Hall, Amherst, MA 01003, USA d Institute for Applied Ecology, Faculty of Applied Science, University of Canberra, Canberra, ACT 2601, Australia a r t i c l e i n f o a b s t r a c t Article history: The red mason bee (Osmia bicornis L.) is a common wild bee in urban environments which contributes Received 12 January 2011 to early-season pollination. We know only little about how any species of wild bee in cities responds Received in revised form 20 May 2011 to resource distribution or landscape structure and the urban habitat(s) that they prefer. We employed Accepted 25 May 2011 a citizen science approach to investigate drivers behind the spatial distribution of this solitary bee in the urban region of Leipzig (Germany). Volunteers hung trap nests at different locations and collected information on eight local, microsite conditions (such as sun exposure, attachment position, local flower Keywords: availability). -
Ptinus Sexpunctatus Panzer (Coleoptera: Anobiidae: Ptininae)
Volume 118, Number 1, January and February 2007 73 PTINUS SEXPUNCTATUS PANZER (COLEOPTERA: ANOBIIDAE, PTININAE) NEWLY RECORDED IN NORTH AMERICA1 Christopher G. Majka,2 T. Keith Philips,3 and Cory Sheffield4 ABSTRACT: The Palearctic spider beetle, Ptinus sexpunctatus Panzer (Anobiidae: Ptininae), is newly recorded in North America from collections in Nova Scotia, Canada, and Pennsylvania and Utah in the United States. It is also newly recorded in association with the native blue orchard mason bee, Osmia lignaria Say (Hymenoptera: Megachilidae). Its presence on the continent is briefly dis- cussed in the context of mechanisms of introduction of invertebrates to North America. KEY WORDS: Ptinus, Anobiidae, Megachilidae, introduced species, new records Ptinus sexpunctatus Panzer is a Palearctic spider beetle (Anobiidae: Ptininae) found from southern Europe north to England and southern Fennoscandia and Karelia; it is also known from the Caucasus (Burakowski et al., 1986) and east to Siberia (Borowski 1996). Within its native range it is an associate of cavity- nesting solitary bees in the genera Osmia and Megachile and also with Hoplitus adunca (Panzer) and Chelostoma nigricorne (Nylander) (Linsley 1942). It is also found in oak (Quercus) forests, particularly in galleries of Cerambyx cerdo Linnaeus (Cerambycidae) (Burakowski et al., 1986). Thirty-eight native and non-native species in the genus Ptinus Linnaeus (spi- der beetles) (Philips 2002) and three additional adventive species not yet report- ed in the literature (Philips, unpublished data) are found in North America. A large number of introduced species in the genera Gibbium Scopoli, Mezium Curtis, Niptus Boildieu, Pseudeurostus Heyden, Sphaericus Wollaston, Tipnus Thompson, and Trigonogenius Solier have also been recorded from North America (Philips 2002). -
Conservation and Management of NORTH AMERICAN MASON BEES
Conservation and Management of NORTH AMERICAN MASON BEES Bruce E. Young Dale F. Schweitzer Nicole A. Sears Margaret F. Ormes Arlington, VA www.natureserve.org September 2015 The views and opinions expressed in this report are those of the author(s). This report was produced in partnership with the U.S. Department of Agriculture, Forest Service. Citation: Young, B. E., D. F. Schweitzer, N. A. Sears, and M. F. Ormes. 2015. Conservation and Management of North American Mason Bees. 21 pp. NatureServe, Arlington, Virginia. © NatureServe 2015 Cover photos: Osmia sp. / Rollin Coville Bee block / Matthew Shepherd, The Xerces Society Osmia coloradensis / Rollin Coville NatureServe 4600 N. Fairfax Dr., 7th Floor Arlington, VA 22203 703-908-1800 www.natureserve.org EXECUTIVE SUMMARY This document provides a brief overview of the diversity, natural history, conservation status, and management of North American mason bees. Mason bees are stingless, solitary bees. They are well known for being efficient pollinators, making them increasingly important components of our ecosystems in light of ongoing declines of honey bees and native pollinators. Although some species remain abundant and widespread, 27% of the 139 native species in North America are at risk, including 14 that have not been recorded for several decades. Threats to mason bees include habitat loss and degradation, diseases, pesticides, climate change, and their intrinsic vulnerability to declines caused by a low reproductive rate and, in many species, small range sizes. Management and conservation recommendations center on protecting suitable nesting habitat where bees spend most of the year, as well as spring foraging habitat. Major recommendations are: • Protect nesting habitat, including dead sticks and wood, and rocky and sandy areas. -
Osmia Cornuta Management in Pear Orchards
Bulletin of Insectology 60 (1): 77-82, 2007 ISSN 1721-8861 Osmia cornuta management in pear orchards 1 1 2 1 Bettina MACCAGNANI , Giovanni BURGIO , Ljubiša Ž. STANISAVLJEVIĆ , Stefano MAINI 1Dipartimento di Scienze e Tecnologie Agroambientali - Entomologia, Università di Bologna, Italy 2Institute of Zoology, Faculty of Biology, University of Belgrade, Serbia Abstract Osmia cornuta (Latreille) (Hymenoptera Megachilidae) is a well-known pollinator of pear and several rosaceous plants. If the pollinator cocoons are released in the orchard at the start of pear flowering and the nests are retrieved at petal fall, both its polli- nating and reproductive potential are limited. The two-years’ study (2004-05) carried out in north-eastern Italy aimed to optimise O. cornuta pollinating potential and to enhance its field reproduction. Two aspects have been considered: 1) the use of ecological infrastructures (hedge of Prunus spinosa L. and strips of Brassica sp.) as complementary food sources in order to anticipate the release of pollinator cocoons in the pear orchard; this technique could allow the female to initiate the nesting/foraging activity be- fore the pear bloom starting; 2) the comparisons among various safety measures to be adopted in order to reduce the impact of noxious treatments; this technique could allow females to extend their activity after pear bloom end. Data showed that both the ecological infrastructures can be functional to anticipate the female activity before pear blooming. The complementary food sources sustained the females and allowed them to increase the number of pedotrophic cells with respect to previous studies con- cerning females released at the beginning of pear blooming. -
Coleoptera, Ptinidae)
Orsis, 9: 77-84 (1994) Nuevos datos taxonÓmicos, corológicos y biológicos sobre Ptinus (Gynopterus)pyrenaeus Pic (Coleoptera, Ptinidae) Xavier Bellés' y Jordi Bosch2 1. Centro de Investigación y Desarrollo (CSIC). Jordi Girona, 18.08034 Barcelona 2. Departament Biologia Animal. Facultat de Biologia. Universitat de Barcelona. Diagonal, 645. 08028 Barcelona Key words: Ptinuspyrenaeus, Ptinus martinezi, host records, nests of Hymenoptera, Megachilidae, parasitism, Anogmus hohenheimensis, Pteromalidae. Abstract. New data on the taxonomy, corology, and biology ofPtinus (Gynoptems) pyrenaeus Pic (Coleoptera, Ptinidae). In this study the synonymy of Ptinus martinezi (Escalera) and Ptinus pyre- naeus Pic is proposed. The area of distribution is expanded with new records from the southern slope of the Pyrenees, the iberian Meseta and Andalusia. From the biological point of view, frequent fin- dings indicate a close association of P. pyrenaeus with nests of solitary hymenoptera (especially Osmia bees), where it behaves as a nest destroyer, feeding on pollen and all sorts of debris found in cells. Anogmus hohenheimensis (Ratzeburg) (Pteromalidae) (cited for the first time in the Iberian Peninsula) is recorded as a parasite of P. pyrenaeus. Resum. Des del punt de vista taxonbrnic es proposa el pas de Ptinus martinezi (Escalera) al qua- dre sinonímic de P. pyrenaeus Pic, i des del punt de vista corolbgic es completa la seva distribució al vessant sud dels Pirineus i s'amplia a la Meseta iberica i Andalusia. Pel que fa a la biologia, s'indi- ca una estreta associació de P. pyrenaeus amb nius d'himenbpters solitaris, sobretot abelles del gkne- re Osmia, on actua m6s aviat com a comensal, alimentant-se de pol.len i d'altres restes trobades a les cel.les. -
Virus Prevalence and Genetic Diversity Across a Wild Bumblebee Community
fmicb-12-650747 April 16, 2021 Time: 19:12 # 1 ORIGINAL RESEARCH published: 22 April 2021 doi: 10.3389/fmicb.2021.650747 Virus Prevalence and Genetic Diversity Across a Wild Bumblebee Community David J. Pascall1,2*, Matthew C. Tinsley3, Bethany L. Clark4,5, Darren J. Obbard6 and Lena Wilfert2,7* 1 Institute of Biodiversity, Animal Health and Comparative Medicine, Boyd Orr Centre for Population and Ecosystem Health, University of Glasgow, Glasgow, United Kingdom, 2 Centre for Ecology and Conservation, University of Exeter, Cornwall, United Kingdom, 3 Biological and Environmental Sciences, University of Stirling, Stirling, United Kingdom, 4 BirdLife International, The David Attenborough Building, Cambridge, United Kingdom, 5 Environment and Sustainability Institute, University of Exeter, Cornwall, United Kingdom, 6 Institute of Evolutionary Biology, University of Edinburgh, Edinburgh, United Kingdom, 7 Institute of Evolutionary Ecology and Conservation Genomics, Ulm University, Ulm, Germany Edited by: Akio Adachi, Viruses are key population regulators, but we have limited knowledge of the diversity Kansai Medical University, Japan and ecology of viruses. This is even the case in wild host populations that provide Reviewed by: ecosystem services, where small fitness effects may have major ecological impacts Scott McArt, Cornell University, United States in aggregate. One such group of hosts are the bumblebees, which have a major Michelle Flenniken, role in the pollination of food crops and have suffered population declines and Montana State University, range contractions in recent decades. In this study, we investigate the diversity of United States four recently discovered bumblebee viruses (Mayfield virus 1, Mayfield virus 2, River *Correspondence: David J. Pascall Liunaeg virus, and Loch Morlich virus), and two previously known viruses that infect [email protected] both wild bumblebees and managed honeybees (Acute bee paralysis virus and Slow Lena Wilfert [email protected] bee paralysis virus) from isolates in Scotland. -
The Spread and Evolution of RNA Viruses Among Honey Bees and the Wider Insect Community with Particular Emphasis on Deformed Wing Virus (DWV)
The spread and evolution of RNA viruses among honey bees and the wider insect community with particular emphasis on Deformed wing virus (DWV). Laura Emily BRETTELL Ph.D. Thesis 2017 School of Environment and Life Sciences University of Salford CONTENTS CONTENTS ............................................................................................................................................... 1 LIST OF FIGURES ...................................................................................................................................... 5 General Introduction........................................................................................................................... 5 Chapter 1: A comparison of Deformed wing virus in deformed and asymptomatic honey bees. ..... 5 Chapter 2: Oldest Varroa tolerant honey bee population provides insight into the origins of the global decline of honey bees. ............................................................................................................. 7 Chapter 4: Moku virus; a new Iflavirus found in wasps, honey bees and Varroa. ............................. 8 Chapter 5: Common apiary pests as a potential source of honey bee – associated viruses in a Hawaiian apiary environment. ............................................................................................................ 9 LIST OF TABLES ...................................................................................................................................... 10 Chapter 1: A comparison -
Identification of New Viruses Specific to the Honey Bee Mite Varroa Destructor
bioRxiv preprint doi: https://doi.org/10.1101/610170; this version posted April 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Identification of new viruses specific to the honey bee mite Varroa destructor Salvador Herrero*, Sandra Coll, Rosa M. González-Martínez, Stefano Parenti, Anabel Millán-Leiva, Joel González-Cabrera* ERI BIOTECMED. Department of Genetics. Universitat de València, Valencia, Spain. *Corresponding authors: Email: [email protected], tel: +34 96 354 3006 Email: [email protected], tel: +34 96 354 3122 Keywords: iflavirus, picornavirus, insobevirus, qPCR, +ssRNA virus 1 bioRxiv preprint doi: https://doi.org/10.1101/610170; this version posted April 16, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Large-scale colony losses among managed Western honey bees have become a serious threat to the beekeeping industry in the last decade. There are multiple factors contributing to these losses but the impact of Varroa destructor parasitism is by far the most important, along with the contribution of some pathogenic viruses vectored by the mite. So far, more than 20 viruses have been identified infecting the honey bee, most of them RNA viruses. They may be maintained either as covert infections or causing severe symptomatic infections, compromising the viability of the colony. In silico analysis of available transcriptomic data obtained from mites collected in the USA and Europe as well as additional investigation with new samples collected locally allowed the description of three novel RNA viruses.