Nitrogen and Phosphorus Fertilization of Sparganium Eurycarpum and Typha Glauca Stands, Eagle Lake, Iowa Robert Kyle Neely Iowa State University

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Nitrogen and Phosphorus Fertilization of Sparganium Eurycarpum and Typha Glauca Stands, Eagle Lake, Iowa Robert Kyle Neely Iowa State University Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1982 Nitrogen and phosphorus fertilization of Sparganium eurycarpum and Typha glauca stands, Eagle Lake, Iowa Robert Kyle Neely Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Botany Commons Recommended Citation Neely, Robert Kyle, "Nitrogen and phosphorus fertilization of Sparganium eurycarpum and Typha glauca stands, Eagle Lake, Iowa " (1982). Retrospective Theses and Dissertations. 7521. https://lib.dr.iastate.edu/rtd/7521 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This reproduction was made from a copy of a document sent to us for microfilming. While the most advanced technology has been used to photograph and reproduce this document, the quality of the reproduction is heavily dependent upon the quality of the material submitted. The following explanation of techniques is provided to help clarify markings or notations which may appear on this reproduction. 1. The sign or "target" for pages apparently lacking from the document photographed is "Missing Page(s)". If it was possible to obtain the missing page(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting through an image and duplicating adjacent pages to assure complete continuity. 2. When an image on the film is obliterated with a round black mark, it is an indication of either blurred copy because of movement during exposure, duplicate copy, or copyrighted materials that should not have been filmed. For blurred pages, a good image of the page can be found in the adjacent frame. If copyrighted materials were deleted, a target note will appear listing the pages in the adjacent frame. 3. When a map, drawing or chart, etc., is part of the material being photographed, a definite method of "sectioning" the material has been followed. It is customary to begin filming at the upper left hand comer of a large sheet and to continue from left to right in equal sections with small overlaps. If necessary, sectioning is continued again—beginning below the first row and continuing on until complete. 4. For illustrations that cannot be satisfactorily reproduced by xerographic means, photographic prints can be purchased at additional cost and inserted into your xerographic copy. These prints are available upon request from the Dissertations Customer Services Department. 5. Some pages in any document may have indistinct print. In all cases the best available copy has been filmed. Universi^ Microrilms Intemationêil 300 N. Zeeb Road Ann Arbor, Ml 48106 8224239 Neely, Robert Kyle NTTROGEN AND PHOSPHORUS FERTILIZATION OF SPARGANIUM EURYCARPUM AND TYPHA GLAUCA STANDS, EAGLE LAKE, IOWA lov/a State University Ph.D. 1982 University Microfilms Intsrnâtionâl m N. Zeeb Road. Ann Arbor. MI 48106 Nitrogen and phosphorus fertilization of Sparganium eurycarpum and Typha glauca stands. Eagle Lake, Iowa •by Robert Kyle Neely A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of the Requirements for the Degree of DOCTOR OF PHILOSOPHY Major: Botany Approved: Signature was redacted for privacy. In Chag^e of Major Work Signature was redacted for privacy. For the Major Departme^ Signature was redacted for privacy. ror the Gradfete College Iowa State University Ames, Iowa 1982 ii TABLE OF CONTENTS Page GENERAL INTRODUCTION 1 Explanation of Thesis Format 2 SECTION I. NITROGEN AND PHOSPHORUS CONCENTRATIONS IN SURFACE AND INTERSTITIAL WATER OF A PRAIRIE MARSH 6 ABSTRACT 7 INTRODUCTION 9 METHODS 11 Study Site 11 Experimental Design 12 Collection and Analysis of Samples 14 Statistical Procedures 16 RESULTS 18 Seasonal Patterns 18 Surface water 18 Nitrogen 18 Phosphorus 20 Interstitial water 23 1979 23 1980 26 Nitrogen 26 Phosphorus 28 Short-Term Patterns 30 Surface water 30 iii 15-cm interstitial water 30 DISCUSSION 33 LITERATURE CITED 38 SECTION II. NUTRIENT LIMITATION OF SPARGANIUM EURYCARPUM AND TYPHA GLAUCA IN A PRAIRIE MARSH 43 ABSTRACT 44 INTRODUCTION 45 METHODS 48 Study Site 48 Construction and Fertilization of Enclosures 49 Collection and Analysis of Samples 49 Sediment and interstitial H^O 49 Vegetation 51 Statistical Analyses 52 RESULTS 54 Interstitial H^O 54 Sediment 56 Above-ground Vegetative Response 58 Nitrogen content 58 Shoot density 60 Biomass and production 60 Below-ground Vegetative Response 66 Nitrogen content 66 Biomass 67 DISCUSSION 69 iv Nutrient Availability 69 Production 70 LITERATURE CITED 74 SECTION III. NITROGEN, PHOSPHORUS, AND THE DECOMPOSITION OF TYPHA GLAUCA AND SPARGANIDM EURYCARPDM IN A PRAIRIE MARSH 80 ABSTRACT 81 INTRODUCTION 83 METHODS 87 Study Site 87 Fertilization Experiments 87 Shoot tissues 88 Root-rhizome tissues 89 Shoot Decomposition and Initial Nutrient Content 89 Statistical Analyses 90 RESULTS 92 Shoot Decomposition 92 Dry weight 92 Nitrogen content 94 Phosphorus content 97 Root-rhizome Decomposition 99 Dry weight 99 Nitrogen content 100 DISCUSSION 102 Dry Weight 102 Shoot litter 102 V Eoot-rhizome litter 105 Nutrient Content 106 Shoot litter 106 Below-ground litter 110 CONCLUSIONS 112 LITERATURE CITED 115 ST3MMARY 120 LITERATURE CITED 123 ACKNOWLEDGEMENTS 125 vi LIST OF FIGURES Page Figure 1. The location of Eagle Lake marsh, Iowa 3 Figure 2. The location of fertilized and unfertilized sampling plots at Eagle Lake marsh 4 Figure 3. The internal arrangement of Eagle Lake sampling plots 5 Figure I-l. The location of fertilized and unfertilized sampling plots at Eagle Lake marsh 13 Figure 1-2. Interstitial water sampling well 15 Figure 1-3. The average concentration of NH^-N, NO^-N, and organic nitrogen in Eagle Lake fertilized and unfertilized surface water during 1979 and 1980 19 Figure 1-4. The average concentration of PO^-P, total dissolved phosphorus (TDP), and particulate phosphorus (PP) in Eagle Lake fertilized and unfertilized surface water in 1979 and 1980 21 Figure 1-5. The average concentration of NH^-N, NO^-N, and organic nitrogen in Eagle Lake fertilized and unfertilized interstitial water at 15-cm and 45-cm depths 27 Figure 1-6. The average concentration of PO.-P and total 4 • phosphorus (TP) in Eagle Lake fertilized and unfertilized interstitial waters at 15-cm and 45-cm depths 29 vii Figure 1-7. The average concentration of NO^-N, and PO^-P in surface water (n=6) and in 15-cm inter­ stitial water (n=2) on successive days after fertilization in July and October Figure II-l. Location of Sparganium and Typha control and fertilized enclosures at each of 3 sites in Eagle Lake marsh. The actual arrangement of plots at each site is not indicated Figure II-2. Average NH^-N, NO^-N, and PO^-P concentrations in interstitial water and sediments of control and fertilized plots during 1979 and 1980 Figure II-3. Average percentage nitrogen in Sparganium and Typha shoot and root-rhizome tissues from con­ trol and fertilized plots during 1979 and 1980 Figure II-4. Average Sparganium and Typha shoot density in control and fertilized plots in 1979 and 1980 Figure II-5. Average Sparganium and Typha total above-ground biomass in control and fertilized plots during 1979 and 1980 Figure II-6. Summary of the 1980 effects of fertilization on Sparganium and Typha stands on NH^-N, NO^-N, and PO^-P concentrations in interstitial waters; NH,-N and PO,-P concentrations in sediments; 4 4 percentage nitrogen in shoots and roots-rhizomes maximum shoot density; net primary production viii (NPP); and autumn below-ground biomass. Signif­ icance at the 0.10 confidence level is desig­ nated by an asterisk. Summer averages are used for all nutrient concentrations 72 Figure III-l. Sources of carbon, nitrogen, and phosphorus for microbial decomposers 84 Figure III-2- The average percentage of original dry weight remaining in submerged Typha and Spar gam'um shoot and root-rhizome litterbags 93 Figure III-3. The average concentration of nitrogen in submerged Typha and Sparganium shoot and root- rhizome litterbags 95 Figure III-4. The average percentage of original nitrogen remaining in submerged Typha and Sparganium shoot and root-rhizome litterbags 96 Figure III-5. The average concentration of phosphorus and the percentage of original phosphorus re­ maining in submerged Typha and Sparganium shoot litterbags 98 Figure 4. The movement and effect of nitrogen and phos­ phorus fertilizer in Eagle Lake marsh. After application, the fertilizer moved through the litter layer into the interstitial water column. As a result, nitrogen and phosphorus concentra­ tions in both compartments were elevated. Emer- ix gent plant uptake and accumulation of nitrogen from interstitial waters in 1979 and 1980 stimu­ lated an increase in shoot density in 1980, an increase in net primary production (NPP) in 1980, and eventually an increase in the rate of decomposition. Percentages denote the 1980 post-fertilization changes in nitrogen and phos­ phorus concentration of interstitial water and live tissues; the change in the absolute quan­ tity of nitrogen and phosphorus in litter; the change in shoot density; the change in NPP; and the change in the rate of decomposition X LIST OF TABLES Page Table I-l. 1979 15-cm interstitial concentrations (mg/1) in Typha glauca and Sparganium eurycarpum experi­ mental plots 24 Table 1-2. 1979 45-cm interstitial concentrations (mg/1) in Typha glauca and Sparganium eurycarpum experi­ mental plots 25 Table II-l. 1979 15-cm interstitial concentrations (ppm) in Typha glauca and Sparganium eurycarpum experi­ mental plots 57 Table II-2. 1979 and 1980 average net annual above-ground 2 production (g/m ), average maximum above-ground 2 biomass (g/m ), average autumn below-ground bio- 2 mass (g/m ), and below-ground:above-ground bio­ mass ratios for Typha glauca and Spar gam'urn eurycarpxmi in control and fertilized plots 64 Table II-3.
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