The Puzzle of Coccoid Forms of Helicobacter Pylori: Beyond Basic Science
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Viable but Non-Culturable Bacteria: Clinical Practice and Future Perspective
Editorial Article 2017, Vol: 5, Issue: 2, Pages: 1-2 DOI: 10.18869/acadpub.rmm.5.2.1 Research in Molecular Medicine Viable but Non-culturable Bacteria: Clinical Practice and Future Perspective Amin Talebi Bezmin Abadi Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Phone: +98-2182884883, E-mail: [email protected] Received: 20 Feb 2017 Revised: 19 Mar 2017 Accepted: 30 Mar 2017 Please cite this article as: Talebi Bezmin Abadi A. Viable but Non-culturable Bacteria: Clinical Practice and Future Perspective. Res Mol Med. 2017; 5 (2): 1-2 Due to intolerable environmental conditions, bacteria learnt to mechanisms to survive VBNC cells in human and environment are adopt a useful strategy to survive longer which these organisms welcomed. generally is termed "viable but non-culturable". In the case of exposure to certain stresses (e.g., antibiotics, low metabolites, Current debate heavy metal and high pH environment, etc.), bacteria enter into the In clinical practices, we should examine possible molecular VBNC stage. In similar, having the starvation mode of physiology approaches for rapid detection of those crucial infectious agents. can be called VBNC model. In fact, viable but non-culturable cells Recently, the potential of microorganisms to enter into the VBNC (VBNC) are live bacteria, however, they are unable to be phase raised the cautious attention of microbiologists and also cultivated on conventional culture media (1). Because of clinicians to rethink about current strategies in hospitals and complexities in culture, observation of normal colonies on solid environmental hygiene. The raised central scientific issue is about and broth media is almost impossible for VBNCs. -
Rapid Urease Test (RUT) for Evaluation of Urease Activity in Oral Bacteria In
Dahlén et al. BMC Oral Health (2018) 18:89 https://doi.org/10.1186/s12903-018-0541-3 RESEARCH ARTICLE Open Access Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo Gunnar Dahlén*, Haidar Hassan, Susanne Blomqvist and Anette Carlén Abstract Background: Urease is an enzyme produced by plaque bacteria hydrolysing urea from saliva and gingival exudate into ammonia in order to regulate the pH in the dental biofilm. The aim of this study was to assess the urease activity among oral bacterial species by using the rapid urease test (RUT) in a micro-plate format and to examine whether this test could be used for measuring the urease activity in site-specific supragingival dental plaque samples ex vivo. Methods: The RUT test is based on 2% urea in peptone broth solution and with phenol red at pH 6.0. Oral bacterial species were tested for their urease activity using 100 μl of RUT test solution in the well of a micro-plate to which a 1 μl amount of cells collected after growth on blood agar plates or in broth, were added. The color change was determined after 15, 30 min, and 1 and 2 h. The reaction was graded in a 4-graded scale (none, weak, medium, strong). Ex vivo evaluation of dental plaque urease activity was tested in supragingival 1 μl plaque samples collected from 4 interproximal sites of front teeth and molars in 18 adult volunteers. The color reaction was read after 1 h in room temperature and scored as in the in vitro test. -
Bacterial Dormancy: a Subpopulation of Viable but Non-Culturable Cells Demonstrates Better Fitness for Revival
bioRxiv preprint doi: https://doi.org/10.1101/2020.07.23.216283; this version posted July 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Bacterial dormancy: a subpopulation of viable but non-culturable cells demonstrates better fitness for revival. Sariqa Wagley1*, Helen Morcrette1, Andrea Kovacs-Simon1, Zheng R. Yang1, Ann Power2, Richard K. Tennant2, John Love2, Neil Murray3, Richard W. Titball1 and Clive S. Butler1* 1 Biosciences, College of Life and Environmental Sciences, University of Exeter, Exeter, Devon, EX4 4QD, UK 2 BioEconomy Centre, The Henry Wellcome Building for BioCatalysis, Biosciences, Stocker Road, Exeter, Devon, EX4 4QD, UK 3 Lyons Seafoods, Fairfield House, Fairfield Road, Warminster, Wiltshire, BA12 9DA. *Corresponding authors: [email protected]; [email protected] Key words: Viable but non culturable cells, Vibrio parahaemolyticus, FACS, IFC, Proteomics Running title: characterisation of subpopulations of VBNC cells bioRxiv preprint doi: https://doi.org/10.1101/2020.07.23.216283; this version posted July 23, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract: The viable but non culturable (VBNC) state is a condition in which bacterial cells are viable and metabolically active, but resistant to cultivation using a routine growth medium. -
Study of the Effect of Post-Operative Medical Management on Peptic Ulcer in Patients of Perforated Peptic Ulcer Disease
International Surgery Journal Deshmukh SB et al. Int Surg J. 2016 Aug;3(3):1267-1272 http://www.ijsurgery.com pISSN 2349-3305 | eISSN 2349-2902 DOI: http://dx.doi.org/10.18203/2349-2902.isj20161459 Research Article Study of the effect of post-operative medical management on peptic ulcer in patients of perforated peptic ulcer disease Sudhir B. Deshmukh1, Koushal U. Kondawar2, Satish Gireboinwd3, Meghraj J. Chawada4* 1Professor and HOD, 2PG Student, 3Assistant Professor, 4Associate Professor, Department of General Surgery, S. R. T. R. Government Medical College, Ambejogai, Maharashtra, India Received: 11 May 2016 Accepted: 14 May 2016 *Correspondence: Dr. Meghraj J. Chawada E-mail: [email protected] Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Background: Peptic ulcer disease remains one of the most prevalent diseases of the gastrointestinal tract with annual incidence 1 ranging from 0.1% to 0.3% in India. Cases of peptic ulcer perforation are commonly encountered in our institute. The objective was to study the effect of post-operative medical management on peptic ulcer in patients of perforated peptic ulcer disease. Methods: A prospective non randomized study was conducted among all diagnosed cases of peptic ulcer perforation patients admitted through emergency or OPD in surgery ward in our hospital. Patient’s case record was evaluated to collect following data: personal information, past history of peptic ulcer disease, use of non-steroidal anti- inflammatory drugs for heart disease or osteoarthritis was taken. -
Discovery and Control of Culturable and Viable but Non-Culturable Cells
www.nature.com/scientificreports OPEN Discovery and control of culturable and viable but non-culturable cells of a distinctive Lactobacillus Received: 20 February 2018 Accepted: 2 July 2018 harbinensis strain from spoiled beer Published: xx xx xxxx Junyan Liu1,3, Yang Deng4, Lin Li1,2,5, Bing Li1,5, Yanyan Li6, Shishui Zhou7, Mark E. Shirtlif8, Zhenbo Xu 1,4,8 & Brian M. Peters3, Occasional beer spoilage incidents caused by false-negative isolation of lactic acid bacteria (LAB) in the viable but non-culturable (VBNC) state, result in signifcant proft loss and pose a major concern in the brewing industry. In this study, both culturable and VBNC cells of an individual Lactobacillus harbinensis strain BM-LH14723 were identifed in one spoiled beer sample by genome sequencing, with the induction and resuscitation of VBNC state for this strain further investigated. Formation of the VBNC state was triggered by low-temperature storage in beer (175 ± 1.4 days) and beer subculturing (25 ± 0.8 subcultures), respectively, and identifed by both traditional staining method and PMA-PCR. Resuscitated cells from the VBNC state were obtained by addition of catalase rather than temperature upshift, changing medium concentration, and adding other chemicals, and both VBNC and resuscitated cells retained similar beer-spoilage capability as exponentially growing cells. In addition to the frst identifcation of both culturable and VBNC cells of an individual L. harbinensis strain from spoiled beer, this study also for the frst time reported the VBNC induction and resuscitation, as well as verifcation of beer-spoilage capability of VBNC and resuscitated cells for the L. -
The Viable but Nonculturable State and Cellular Resuscitation
To Grow or Not to Grow: Nonculturability Revisited The viable but nonculturable state and cellular resuscitation J.D. Oliver University of North Carolina, Charlotte, NC 28223 USA ABSTRACT Aquatic microbial ecologists have long recognized that portions of bacterial populations seem to disappear from natural water bodies during certain times of the year, only to reappear at other times. In the case of V. vulnificus, numerous studies have documented the difficulty researchers experience in culturing this bacterium during cold months, presumably due to “die-off” of the population. This decrease in culturability is thought to be due to the entrance of the cells into a viable but nonculturable (VBNC) state, reported to occur in at east 30 other bacterial species. It is thought that the VBNC state may represent a survival response of bacteria exposed to stressful environmental conditions and, in the case of V. vulnificus, is induced by temperatures below 10 oC. The ability of any bacterium to resuscitate from this dormant state would appear to be essential if the VBNC state were truly a survival strategy. Whether the culturable cells, which appear following stress removal, are a result of true resuscitation, or from re-growth of a few residual culturable cells, has long been debated. Our research, including dilution studies, time required for resuscitation to occur, and the effects of nutrient on recovery, indicates that, at least for V. vulnificus, true resuscitation does occur. Our studies have further suggested that nutrient is in some way inhibitory to the resuscitation of cells in the VBNC state, and that studies which add nutrient in an attempt to detect resuscitation are only able to detect culturable cells which might be present. -
Spiral and Atypical Bacteria, and Legionella. Answer Questions
Lecture 7: Spiral and atypical bacteria, and Legionella. Answer questions: 1. Name flexible and nonflexible spiral bacteria. 2. What is axial filament (endoflagella)? What are difference in the structure of flexible and nonflexible spiral bacteria? 3. Name virulence factors of flexible spiral bacteria 4. Name Leptospira species pathogenic to humans 5. What is the reservoir of Leptospira? How these bacteria are transmitted to humans? 6. Name diseases produced by Leptospira interrogans 7. Name Borrelia species associated with endemic and epidemic relapsing fever. Indicate their reservoirs and ways of transmission to humans 8. Name Borrelia species causing borreliosis (Lyme disease). What is their reservoir and how they are transmitted to humans? 9. What are vectors transmitting diseases caused by Borrelia species to humans? 10. Name most common clinical symptoms of borreliosis: dermatological, rheumatic, cardiac and neurological 11. Name pathogenic and nonpathogenic species of Treponema 12. What are bejel, yaws and pinta? 13. What is etiologic agent of syphilis? How it is transmitted to humans? What is the reservoir of the disease? 14. Name stages of syphilis and indicate how long they last? 15. Describe main clinical symptoms of each stage of syphilis 16. Why syphilis is considered devastating disease? 17. What are the main clinical syndroms of congenital syphilis? 18. What is the reservoir of Helicobacter pylori? What are virulence factors of the pathogen? How the pathogen is transmitted to humans? 19. Explain patomechanism of H. pylori infection 20. What are virulence factors of H. pylori? 21. Name diseases caused by H. pylori 22. Name Campylobacter species pathogenic to humans. What is the reservoir of these bacteria? How they are transmitted to humans? 23. -
Helicobacter Pylori Infections: Culture from Stomach Biopsy, Rapid Urease Test (Cutest®), and Histologic Examination of Gastric Biopsy
Available online at www.annclinlabsci.org 148 Annals of Clinical & Laboratory Science, vol. 45, no. 2, 2015 An Efficiency Comparison between Three Invasive Methods for the Diagnosis of Helicobacter pylori Infections: Culture from Stomach Biopsy, Rapid Urease Test (CUTest®), and Histologic Examination of Gastric Biopsy Avi Peretz1, Avi On 2, Anna Koifman1, Diana Brodsky1, Natlya Isakovich1, Tatyana Glyatman1, and Maya Paritsky3 1Clinical Microbiology Laboratory, 2Pediatric Gastrointestinal Unit, and 3Gastrointestinal Unit, Baruch Padeh Medical Center, Poria, affiliated to the Faculty of Medicine, Bar Ilan University, Galille, Israel Abstract. Background. Helicobacter pylori is one of the most prevalent pathogenic bacteria in the world, and humans are its principal reservoir. There are several available methods to diagnose H. pylori infection. Disagreement exists as to the best and most efficient method for diagnosis. Methods. In this paper, we report the results of a comparison between three invasive methods for H. pylori diagnosis among 193 pa- tients: culture, biopsy for histologic examination, and rapid urease test (CUTest®). Results. We found that all three methods have a high sensitivity and specificity for the diagnosis of infections caused by H. pylori. However, the culture method, which is not used routinely, also showed high sensitivity, probably due to biopsies’ seeding within 30 minutes, using warm culture media, non-selective media, and longer incuba- tion. Conclusions. Although not a routine test, culture from biopsy can be meaningful in identification of antibiotic-resistant strains of H. pylori and should therefore be considered a useful diagnostic tool. Keywords: Helicobacter pylor, Culture, Urease test, Gastric biopsy. Introduction Helicobacter pylori is one of the most prevalent Recently, a close association was found between H. -
Do Perforated Gastric Ulcers Require Routine
DO PERFORATED GASTRIC ULCERS REQUIRE ROUTINE INTRA-OPERATIVE BIOPSY? Meryl Dache Oyomno A research report submitted to the Faculty of Health Sciences, University of the Witwatersrand, in fulfillment of the requirements for the degree of Master of Medicine (General Surgery). Johannesburg, 2018 i DECLARATION I, Meryl Dache Oyomno, declare that this research report is my own work. It is being submitted for the degree of Master of Medicine (General Surgery) at the University of the Witwatersrand, Johannesburg. It has not been submitted before either whole or in part for any degree or examination at this or in any other university. ………………………………. On the ……. day of………. 2017 ii DEDICATION To my parents, Violet and Gordon Oyomno. iii ACKNOWLEDGEMENTS I owe a great debt of gratitude to Dr. Martin Brand, a remarkable supervisor! He was an enormous help in the formulation of the research question and protocol and read multiple drafts of the written report. I appreciate the quick responses and helpful feedback, the guidance and expert advice. In the same breath, I would like to thank Professor Damon Bizos for not only helping me come up with the research topic but for his constant guidance and support. I would also like thank the Wits postgraduate Hub statistical consultation team and Dr. Petra Gaylard of Data management and statistical analysis DMSA for their assistance in the data analysis and statistics. iv PRESENTATIONS ARISING FROM RESEARCH REPORT Oral Presentation 1. Do perforated gastric ulcers require routine intra-operative biopsy? A study in three Gauteng public hospitals. Bert Myburgh Research Forum 2015 v ABSTRACT Background. -
The Molecular Phylogeny and Ecology of Spiral Bacteria from the Mouse Gastrointestinal Tract
The Molecular Phylogeny and Ecology of Spiral Bacteria from the Mouse Gastrointestinal Tract Bronwyn Ruth Robertson A thesis submitted for the degree of Doctor of Philosophy School of Microbiology and Immunology The University of New South Wales Sydney, Australia May, 1998 'Brief rejfection on test-tu.ies 'Ta~ a piece offire, a piece ofwater, a piece of ra66it or a piece of tree, or any piece ofa liuman 6eing, ~ it, slia~ it, stopper it up, k.._eep it wann, in tlie tfarl<:.i in tlie Bglit, refrigerate/, fet it stantf stifffor a wliife - yourselves far from stiff- 6ut that's tlie realjo~. Jtjter a wliife you wok.._- ~ntf it's growing, a fittfe ocean, a fittle vofcano, a fittfe tree, a fittfe lieart, a fittfe 6rain, so fittfe you don't liear it lamenting as it wants to get out, 6ut that's tlie reafjo~, not liearing it. 'Ift.engo ·antf record it, a[[ tfaslies or a[[ crosses, some witli ~famation-mar/&, a[[ nouglits antf a[[figures, some witli ~famation-marf&, antf that's tlie reafjo~, in effect a test-tu6e is a device for changing nouglits into ~famation mar/&. 'Iliat's tlie reafJo~ wliicli mak.._es you forget for a wliile tliat reaffy you yourself are In tlie test-tu6e Mirosfav !Jfo{u6 Poems 'Before arufJtfter Acknowledgements I extend my grateful thanks to the following people for their assistance and encouragement during my PhD studies. Professor Adrian Lee for giving me the opportunity to carry out my PhD in his laboratory, for his supervision and for his enthusiasm for the "other helicobacters". -
Are the View of Helicobacter Pylori Colonized in the Oral Cavity an Illusion?
OPEN Experimental & Molecular Medicine (2017) 49, e397; doi:10.1038/emm.2017.225 Official journal of the Korean Society for Biochemistry and Molecular Biology www.nature.com/emm REVIEW Are the view of Helicobacter pylori colonized in the oral cavity an illusion? JKC Yee Urea breath test (UBT), as a leading preferred non-invasive diagnostic technology, but may not be able to detect oral H. pylori. With negative results of UBT, the patient may have an oral infection. On the basis of the fact of success, eradication rate may increase by 21% in the 95% Cl range after the elimination of oral H. pylori, the author believes oral H. pylori does exist and the oral cavity is the second colonized site aside its primary site of the stomach. H. pylori migrated out of Africa along with its human host circa 60 000 years ago; they are not lives in stomach only. In this review article, evidence established in recent years studies with use more appropriate technology had been listed and discussed. The author considers the oral cavity is a black hole for H. pylori infection that significant effective on gastroenterology and another medical field. The role of the oral cavity as the source of H. pylori infection is so controvert in past years. It seems like a human being having a second-time face to discover H. pylori in the history. Experimental & Molecular Medicine (2017) 49, e397; doi:10.1038/emm.2017.225; published online 24 November 2017 INTRODUCTION because the majority of physicians and scientists in this field do Most scientists in this field proposed there are no living not consider oral H. -
Lecture 4 Bacteria and Their Structure Introduction of Bacteria Shapes Of
Lecture 4 Bacteria and their Structure Introduction of bacteria Bacteria are single celled prokaryotic unicellular microorganisms, usually a few micrometers in length that normally exist together in millions. The cell wall of bacteria usually contains peptidoglycan and multiplies by binary fission. The cell structure is simpler than that of other organisms as there is no nucleus or membrane bound organelles. Instead their control Centre containing the genetic information is contained in a single loop of DNA. Some bacteria have an extra circle of genetic material called a plasmid. The plasmid often contains genes that give the bacterium some advantage over other bacteria. For example it may contain a gene that makes the bacterium resistant to a certain antibiotic. Shapes of bacteria Most bacteria are 0.2 um in diameter and 2-8 um in length. The three basic bacterial shapes are coccus (spherical), bacillus (rod-shaped), and spiral (vibrio twisted), however pleomorphic bacteria can assume several shapes. Characteristic Groups These bacteria can give themselves higher Level structural organizations such as Cocci Cocci may be oval, elongated, or flattened on one side. Cocci may remain attached after cell division. These group characteristics are often used to help identify certain cocci. 1) Cocci that remain in pairs after dividing are called diplococci. 2) Cocci that remain in chains after dividing are called streptococci. 3) Cocci that divide in two planes and remain in groups of four are called tetrads. 4) Cocci that divide in three planes and remain in groups cube like groups of eight are called sarcinae. 5) Cocci that divide in multiple planes and form grape like clusters or sheets are called staphylococci.