Order: [email protected] Support: [email protected] Web: www.enogene.com E 2200 764 CRK Antibody

Catalog Number: E2200764 Size: 100ug Host: Mouse Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol Sensitivity: This antibody detects endogenous levels of CRK and does not cross-react with related . Entrez summary: This gene encodes a member of an adapter family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. UniPort summary The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming Function: activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. Immunogen: Purified recombinant human CRK protein fragments expressed in E.coli. Antibody Type: Monoclonal antibody Isotype: IgG2b Purified method: Affinity purified Subcellular location: Cytoplasm, Reactivity: H Applications: WB Molecular Weight: 34kDa UniProt number: P46108 GeneBank ID: NM_016823.2 Gene symbol: p38; CRKII Alternate names: Proto-oncogene c-Crk; p38;CRKII;

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Western blot detection of CRK in LNCAP,A431，MCF7&HT1080 cell lysates using CRK antibody (1:1000 diluted). Predicted band size: 34kDa Observed band size: 34kDa.

For Research Use Only